Molecular networking based LC/MS reveals novel biotransformation products of green coffee by ex vivo cultures of the human gut microbiome
Introduction Unroasted green coffee bean is an increasingly popular beverage and weight loss supplement that contains higher levels of chlorogenic acid derivatives and lower alkaloid levels than roasted beans. Nonetheless, how the gut microbiome metabolizes green coffee constituents has not been stu...
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| Veröffentlicht in: | Metabolomics 2020-08, Vol.16 (8), p.86-86, Article 86 |
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| creator | Farag, Mohamed A. Hegazi, Nesrine M. Donia, Mohamed S. |
| description | Introduction
Unroasted green coffee bean is an increasingly popular beverage and weight loss supplement that contains higher levels of chlorogenic acid derivatives and lower alkaloid levels than roasted beans. Nonetheless, how the gut microbiome metabolizes green coffee constituents has not been studied.
Objectives
To identify possible biotransformation products of green coffee extract by the human gut microbiome, and the potential implications of this process on its biological effects or fate inside the body.
Methods
Molecular networking via the GNPS platform was employed for the visualization of green coffee metabolite profiles acquired using LC-tandem mass spectrometry post-incubation with an ex vivo culture of the human gut microbiome.
Results
36 Metabolites were annotated including four unreported alkyl cinnamate esters in green coffee along with six novel biotransformation products.
Conclusion
Our finding reveals new biotransformation products of cinnamate esters by the gut microbiome mediated via oxidative reactions such as dehydrogenation and hydroxylation, along with methylation, decarboxylation, and deglycosylation. These findings reveal potential interactions between the gut microbiome and green coffee constituents, and paves the way towards studying the effects of these interactions on both microbiome and the human host.
Graphic abstract |
| doi_str_mv | 10.1007/s11306-020-01704-z |
| format | Article |
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Unroasted green coffee bean is an increasingly popular beverage and weight loss supplement that contains higher levels of chlorogenic acid derivatives and lower alkaloid levels than roasted beans. Nonetheless, how the gut microbiome metabolizes green coffee constituents has not been studied.
Objectives
To identify possible biotransformation products of green coffee extract by the human gut microbiome, and the potential implications of this process on its biological effects or fate inside the body.
Methods
Molecular networking via the GNPS platform was employed for the visualization of green coffee metabolite profiles acquired using LC-tandem mass spectrometry post-incubation with an ex vivo culture of the human gut microbiome.
Results
36 Metabolites were annotated including four unreported alkyl cinnamate esters in green coffee along with six novel biotransformation products.
Conclusion
Our finding reveals new biotransformation products of cinnamate esters by the gut microbiome mediated via oxidative reactions such as dehydrogenation and hydroxylation, along with methylation, decarboxylation, and deglycosylation. These findings reveal potential interactions between the gut microbiome and green coffee constituents, and paves the way towards studying the effects of these interactions on both microbiome and the human host.
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Unroasted green coffee bean is an increasingly popular beverage and weight loss supplement that contains higher levels of chlorogenic acid derivatives and lower alkaloid levels than roasted beans. Nonetheless, how the gut microbiome metabolizes green coffee constituents has not been studied.
Objectives
To identify possible biotransformation products of green coffee extract by the human gut microbiome, and the potential implications of this process on its biological effects or fate inside the body.
Methods
Molecular networking via the GNPS platform was employed for the visualization of green coffee metabolite profiles acquired using LC-tandem mass spectrometry post-incubation with an ex vivo culture of the human gut microbiome.
Results
36 Metabolites were annotated including four unreported alkyl cinnamate esters in green coffee along with six novel biotransformation products.
Conclusion
Our finding reveals new biotransformation products of cinnamate esters by the gut microbiome mediated via oxidative reactions such as dehydrogenation and hydroxylation, along with methylation, decarboxylation, and deglycosylation. These findings reveal potential interactions between the gut microbiome and green coffee constituents, and paves the way towards studying the effects of these interactions on both microbiome and the human host.
Graphic abstract</description><subject>Beans</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biotransformation</subject><subject>Body weight loss</subject><subject>Cell Biology</subject><subject>Chlorogenic acid</subject><subject>Coffee</subject><subject>Decarboxylation</subject><subject>Deglycosylation</subject><subject>Dehydrogenation</subject><subject>Developmental Biology</subject><subject>Digestive system</subject><subject>Esters</subject><subject>Hydroxylation</subject><subject>Intestinal microflora</subject><subject>Life Sciences</subject><subject>Lobbying</subject><subject>Mass spectroscopy</subject><subject>Metabolites</subject><subject>Methylation</subject><subject>Microbiomes</subject><subject>Microbiota</subject><subject>Molecular Medicine</subject><subject>Original Article</subject><issn>1573-3882</issn><issn>1573-3890</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kc1uFDEQhEcIJEKSF8ipJS5chrTt8fwc0Yo_aaMcCGfL9rQ3E2bsYHsWkjfgrXGyKEgcOHUfvqrqVlXVGcO3DLE7T4wJbGvkWCPrsKnvn1VHTHaiFv2Az5_2nr-sXqV0g9g0Q4dH1a-LMJNdZx3BU_4R4rfJ78DoRCNsN-cXXyDSnvScwIc9zWCmkKP2yYW46DwFD7cxjKvNCYKDXSTyYINzRGDugH7CftoHKAF5jfTI5GuC63XRHnZrhmWyMRTThU6qF67k0OmfeVx9_fD-avOp3l5-_Lx5t62tkDzXLY3a8h4HidZIZzvSLWd6cE3LG2fQ9Ea2TPZOyqEZpe6MI8l1a4QbjWSdOK7eHHzL4d9XSlktU7I0z9pTWJPijUDR9m3fFPT1P-hNWKMv1xWKDwNnA3-g-IEqn6QUyanbOC063imG6qEddWhHlXbUYzvqvojEQZQK7HcU_1r_R_UbTNeVcw</recordid><startdate>20200801</startdate><enddate>20200801</enddate><creator>Farag, Mohamed A.</creator><creator>Hegazi, Nesrine M.</creator><creator>Donia, Mohamed S.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PKEHL</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5139-1863</orcidid></search><sort><creationdate>20200801</creationdate><title>Molecular networking based LC/MS reveals novel biotransformation products of green coffee by ex vivo cultures of the human gut microbiome</title><author>Farag, Mohamed A. ; Hegazi, Nesrine M. ; Donia, Mohamed S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-6edac280950cb5fc7ea621a9f4624fb0b8b56158f5594d5a7bfe52a6b3fdb5173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Beans</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biotransformation</topic><topic>Body weight loss</topic><topic>Cell Biology</topic><topic>Chlorogenic acid</topic><topic>Coffee</topic><topic>Decarboxylation</topic><topic>Deglycosylation</topic><topic>Dehydrogenation</topic><topic>Developmental Biology</topic><topic>Digestive system</topic><topic>Esters</topic><topic>Hydroxylation</topic><topic>Intestinal microflora</topic><topic>Life Sciences</topic><topic>Lobbying</topic><topic>Mass spectroscopy</topic><topic>Metabolites</topic><topic>Methylation</topic><topic>Microbiomes</topic><topic>Microbiota</topic><topic>Molecular Medicine</topic><topic>Original Article</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Farag, Mohamed A.</creatorcontrib><creatorcontrib>Hegazi, Nesrine M.</creatorcontrib><creatorcontrib>Donia, Mohamed S.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Metabolomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Farag, Mohamed A.</au><au>Hegazi, Nesrine M.</au><au>Donia, Mohamed S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular networking based LC/MS reveals novel biotransformation products of green coffee by ex vivo cultures of the human gut microbiome</atitle><jtitle>Metabolomics</jtitle><stitle>Metabolomics</stitle><date>2020-08-01</date><risdate>2020</risdate><volume>16</volume><issue>8</issue><spage>86</spage><epage>86</epage><pages>86-86</pages><artnum>86</artnum><issn>1573-3882</issn><eissn>1573-3890</eissn><abstract>Introduction
Unroasted green coffee bean is an increasingly popular beverage and weight loss supplement that contains higher levels of chlorogenic acid derivatives and lower alkaloid levels than roasted beans. Nonetheless, how the gut microbiome metabolizes green coffee constituents has not been studied.
Objectives
To identify possible biotransformation products of green coffee extract by the human gut microbiome, and the potential implications of this process on its biological effects or fate inside the body.
Methods
Molecular networking via the GNPS platform was employed for the visualization of green coffee metabolite profiles acquired using LC-tandem mass spectrometry post-incubation with an ex vivo culture of the human gut microbiome.
Results
36 Metabolites were annotated including four unreported alkyl cinnamate esters in green coffee along with six novel biotransformation products.
Conclusion
Our finding reveals new biotransformation products of cinnamate esters by the gut microbiome mediated via oxidative reactions such as dehydrogenation and hydroxylation, along with methylation, decarboxylation, and deglycosylation. These findings reveal potential interactions between the gut microbiome and green coffee constituents, and paves the way towards studying the effects of these interactions on both microbiome and the human host.
Graphic abstract</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s11306-020-01704-z</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-5139-1863</orcidid></addata></record> |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Beans Biochemistry Biomedical and Life Sciences Biomedicine Biotransformation Body weight loss Cell Biology Chlorogenic acid Coffee Decarboxylation Deglycosylation Dehydrogenation Developmental Biology Digestive system Esters Hydroxylation Intestinal microflora Life Sciences Lobbying Mass spectroscopy Metabolites Methylation Microbiomes Microbiota Molecular Medicine Original Article |
| title | Molecular networking based LC/MS reveals novel biotransformation products of green coffee by ex vivo cultures of the human gut microbiome |
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