Enhanced exopolysaccharide production in submerged fermentation of Ganoderma lucidum by Tween 80 supplementation
Bioactive polysaccharides extracted from Ganoderma lucidum ( G. lucidum ) have been widely applied in food and medicine for their multiple functions. In this study, G. lucidum exopolysaccharide (EPS) production in submerged fermentation was stimulated by Tween 80. The addition of 0.25% Tween 80 on d...
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| Veröffentlicht in: | Bioprocess and biosystems engineering 2021, Vol.44 (1), p.47-56 |
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| creator | Yang, Xiaobing Yang, Yingyin Zhang, Yifan He, Jiahao Xie, Yizhen |
| description | Bioactive polysaccharides extracted from
Ganoderma lucidum
(
G. lucidum
) have been widely applied in food and medicine for their multiple functions. In this study,
G. lucidum
exopolysaccharide (EPS) production in submerged fermentation was stimulated by Tween 80. The addition of 0.25% Tween 80 on day 3 gave a maximum production of mycelial biomass and EPS, with an increase of 19.76 and 137.50%, respectively. Analysis of fermentation kinetics showed that glucose was consumed faster after adding Tween 80, while the expression of EPS biosynthesis-related genes and ATP generation were greatly improved. Moreover, Tween 80 resulted in the significant accumulation of reactive oxygen species and increased cell membrane and cell wall permeability. The EPS from Tween 80-containing medium had higher contents of carbohydrate and uronic acid, lower molecular weight, and higher antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals than those of EPS produced in the absence of Tween 80. This study provides further evidence to clarify the stimulatory effects of Tween 80 in fermentation and provides a guide for the production of bioactive
G. lucidum
EPS. |
| doi_str_mv | 10.1007/s00449-020-02418-1 |
| format | Article |
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Ganoderma lucidum
(
G. lucidum
) have been widely applied in food and medicine for their multiple functions. In this study,
G. lucidum
exopolysaccharide (EPS) production in submerged fermentation was stimulated by Tween 80. The addition of 0.25% Tween 80 on day 3 gave a maximum production of mycelial biomass and EPS, with an increase of 19.76 and 137.50%, respectively. Analysis of fermentation kinetics showed that glucose was consumed faster after adding Tween 80, while the expression of EPS biosynthesis-related genes and ATP generation were greatly improved. Moreover, Tween 80 resulted in the significant accumulation of reactive oxygen species and increased cell membrane and cell wall permeability. The EPS from Tween 80-containing medium had higher contents of carbohydrate and uronic acid, lower molecular weight, and higher antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals than those of EPS produced in the absence of Tween 80. This study provides further evidence to clarify the stimulatory effects of Tween 80 in fermentation and provides a guide for the production of bioactive
G. lucidum
EPS.</description><identifier>ISSN: 1615-7591</identifier><identifier>EISSN: 1615-7605</identifier><identifier>DOI: 10.1007/s00449-020-02418-1</identifier><identifier>PMID: 32743719</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Antioxidants ; Biological activity ; Biosynthesis ; Biotechnology ; Carbohydrates ; Cell membranes ; Cell walls ; Chemistry ; Chemistry and Materials Science ; Dietary supplements ; Environmental Engineering/Biotechnology ; Exopolysaccharides ; Fermentation ; Food Science ; Ganoderma lucidum ; Gene expression ; Industrial and Production Engineering ; Industrial Chemistry/Chemical Engineering ; Membrane permeability ; Molecular weight ; Mushrooms ; Mycelia ; Polysaccharides ; Reactive oxygen species ; Research Paper ; Saccharides ; Uronic acid</subject><ispartof>Bioprocess and biosystems engineering, 2021, Vol.44 (1), p.47-56</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3271-76acf3b76e865a162d21c3439bbefe27a37aa085e9237541233e291fff875bbb3</citedby><cites>FETCH-LOGICAL-c3271-76acf3b76e865a162d21c3439bbefe27a37aa085e9237541233e291fff875bbb3</cites><orcidid>0000-0002-9753-0010</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00449-020-02418-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00449-020-02418-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32743719$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Xiaobing</creatorcontrib><creatorcontrib>Yang, Yingyin</creatorcontrib><creatorcontrib>Zhang, Yifan</creatorcontrib><creatorcontrib>He, Jiahao</creatorcontrib><creatorcontrib>Xie, Yizhen</creatorcontrib><title>Enhanced exopolysaccharide production in submerged fermentation of Ganoderma lucidum by Tween 80 supplementation</title><title>Bioprocess and biosystems engineering</title><addtitle>Bioprocess Biosyst Eng</addtitle><addtitle>Bioprocess Biosyst Eng</addtitle><description>Bioactive polysaccharides extracted from
Ganoderma lucidum
(
G. lucidum
) have been widely applied in food and medicine for their multiple functions. In this study,
G. lucidum
exopolysaccharide (EPS) production in submerged fermentation was stimulated by Tween 80. The addition of 0.25% Tween 80 on day 3 gave a maximum production of mycelial biomass and EPS, with an increase of 19.76 and 137.50%, respectively. Analysis of fermentation kinetics showed that glucose was consumed faster after adding Tween 80, while the expression of EPS biosynthesis-related genes and ATP generation were greatly improved. Moreover, Tween 80 resulted in the significant accumulation of reactive oxygen species and increased cell membrane and cell wall permeability. The EPS from Tween 80-containing medium had higher contents of carbohydrate and uronic acid, lower molecular weight, and higher antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals than those of EPS produced in the absence of Tween 80. This study provides further evidence to clarify the stimulatory effects of Tween 80 in fermentation and provides a guide for the production of bioactive
G. lucidum
EPS.</description><subject>Antioxidants</subject><subject>Biological activity</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Carbohydrates</subject><subject>Cell membranes</subject><subject>Cell walls</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Dietary supplements</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Exopolysaccharides</subject><subject>Fermentation</subject><subject>Food Science</subject><subject>Ganoderma lucidum</subject><subject>Gene expression</subject><subject>Industrial and Production Engineering</subject><subject>Industrial Chemistry/Chemical Engineering</subject><subject>Membrane permeability</subject><subject>Molecular weight</subject><subject>Mushrooms</subject><subject>Mycelia</subject><subject>Polysaccharides</subject><subject>Reactive oxygen species</subject><subject>Research Paper</subject><subject>Saccharides</subject><subject>Uronic acid</subject><issn>1615-7591</issn><issn>1615-7605</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kc1O3TAQha2qqPy0L8ACWeqmm9AZO4njJUKUIiF1A2vLccYQlNjBvlF7376GC1TqgoVly_Od4xkfxo4RThFAfc8Ada0rEFBWjV2FH9gBtthUqoXm4-u50bjPDnN-AMCmE_CJ7UuhaqlQH7DlItzb4Gjg9Ccucdpm69y9TeNAfElxWN1mjIGPgee1nyndFdJTmils7HMlen5pQxzKneXT6sZhnXm_5Te_iQLvoOiWZaI3wWe25-2U6cvLfsRuf1zcnP-srn9dXp2fXVeuNIdlAuu87FVLXdtYbMUg0Mla6r4nT0JZqayFriEtpGpqFFKS0Oi971TT9708Yt92vmWKx5XyxsxjdjRNNlBcsxG1BNAShS7o1__Qh7imULorlNICsK1VocSOcinmnMibJY2zTVuDYJ7yMLs8TMnDPOdhsIhOXqyffm94k7wGUAC5A3IphTtK_95-x_YvV2CWhQ</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Yang, Xiaobing</creator><creator>Yang, Yingyin</creator><creator>Zhang, Yifan</creator><creator>He, Jiahao</creator><creator>Xie, Yizhen</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9753-0010</orcidid></search><sort><creationdate>2021</creationdate><title>Enhanced exopolysaccharide production in submerged fermentation of Ganoderma lucidum by Tween 80 supplementation</title><author>Yang, Xiaobing ; Yang, Yingyin ; Zhang, Yifan ; He, Jiahao ; Xie, Yizhen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3271-76acf3b76e865a162d21c3439bbefe27a37aa085e9237541233e291fff875bbb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antioxidants</topic><topic>Biological activity</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Carbohydrates</topic><topic>Cell membranes</topic><topic>Cell walls</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Dietary supplements</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Exopolysaccharides</topic><topic>Fermentation</topic><topic>Food Science</topic><topic>Ganoderma lucidum</topic><topic>Gene expression</topic><topic>Industrial and Production Engineering</topic><topic>Industrial Chemistry/Chemical Engineering</topic><topic>Membrane permeability</topic><topic>Molecular weight</topic><topic>Mushrooms</topic><topic>Mycelia</topic><topic>Polysaccharides</topic><topic>Reactive oxygen species</topic><topic>Research Paper</topic><topic>Saccharides</topic><topic>Uronic acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Xiaobing</creatorcontrib><creatorcontrib>Yang, Yingyin</creatorcontrib><creatorcontrib>Zhang, Yifan</creatorcontrib><creatorcontrib>He, Jiahao</creatorcontrib><creatorcontrib>Xie, Yizhen</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Bioprocess and biosystems engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Xiaobing</au><au>Yang, Yingyin</au><au>Zhang, Yifan</au><au>He, Jiahao</au><au>Xie, Yizhen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced exopolysaccharide production in submerged fermentation of Ganoderma lucidum by Tween 80 supplementation</atitle><jtitle>Bioprocess and biosystems engineering</jtitle><stitle>Bioprocess Biosyst Eng</stitle><addtitle>Bioprocess Biosyst Eng</addtitle><date>2021</date><risdate>2021</risdate><volume>44</volume><issue>1</issue><spage>47</spage><epage>56</epage><pages>47-56</pages><issn>1615-7591</issn><eissn>1615-7605</eissn><abstract>Bioactive polysaccharides extracted from
Ganoderma lucidum
(
G. lucidum
) have been widely applied in food and medicine for their multiple functions. In this study,
G. lucidum
exopolysaccharide (EPS) production in submerged fermentation was stimulated by Tween 80. The addition of 0.25% Tween 80 on day 3 gave a maximum production of mycelial biomass and EPS, with an increase of 19.76 and 137.50%, respectively. Analysis of fermentation kinetics showed that glucose was consumed faster after adding Tween 80, while the expression of EPS biosynthesis-related genes and ATP generation were greatly improved. Moreover, Tween 80 resulted in the significant accumulation of reactive oxygen species and increased cell membrane and cell wall permeability. The EPS from Tween 80-containing medium had higher contents of carbohydrate and uronic acid, lower molecular weight, and higher antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals than those of EPS produced in the absence of Tween 80. This study provides further evidence to clarify the stimulatory effects of Tween 80 in fermentation and provides a guide for the production of bioactive
G. lucidum
EPS.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32743719</pmid><doi>10.1007/s00449-020-02418-1</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-9753-0010</orcidid></addata></record> |
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| subjects | Antioxidants Biological activity Biosynthesis Biotechnology Carbohydrates Cell membranes Cell walls Chemistry Chemistry and Materials Science Dietary supplements Environmental Engineering/Biotechnology Exopolysaccharides Fermentation Food Science Ganoderma lucidum Gene expression Industrial and Production Engineering Industrial Chemistry/Chemical Engineering Membrane permeability Molecular weight Mushrooms Mycelia Polysaccharides Reactive oxygen species Research Paper Saccharides Uronic acid |
| title | Enhanced exopolysaccharide production in submerged fermentation of Ganoderma lucidum by Tween 80 supplementation |
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