Multiscale high-speed photoacoustic microscopy based on free-space light transmission and a MEMS scanning mirror
The conventional photoacoustic microscopy (PAM) system allows trade-offs between lateral resolution and imaging depth, limiting its applications in biological imaging in vivo . Here we present an integrated optical-resolution (OR) and acoustic-resolution (AR) multiscale PAM based on free-space light...
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| Veröffentlicht in: | Optics letters 2020-08, Vol.45 (15), p.4312-4315 |
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| container_title | Optics letters |
| container_volume | 45 |
| creator | Zhang, Chen Zhao, Huangxuan Xu, Song Chen, Ningbo Li, Ke Jiang, Xinkuan Liu, Liangjian Liu, Zhicheng Wang, Lidai Wong, Kenneth K. Y. Zou, Jun Liu, Chengbo Song, Liang |
| description | The conventional photoacoustic microscopy (PAM) system allows trade-offs between lateral resolution and imaging depth, limiting its applications in biological imaging
in vivo
. Here we present an integrated optical-resolution (OR) and acoustic-resolution (AR) multiscale PAM based on free-space light transmission and fast microelectromechanical systems (MEMS) scanning. The lateral resolution for OR is 4.9 µm, and the lateral resolution for AR is 114.5 µm. The maximum imaging depth for OR is 0.7 mm, and the maximum imaging depth for AR is 4.1 mm. The imaging speed can reach 50 k Alines per second. The high signal-to-noise ratios and wavelength throughput are achieved by delivering light via free-space, and the high speed is achieved by a MEMS scanning mirror. The blood vasculature from superficial skin to the deep tissue of a mouse leg was imaged
in vivo
using two different resolutions to demonstrate the multiscale imaging capability. |
| doi_str_mv | 10.1364/OL.397733 |
| format | Article |
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in vivo
. Here we present an integrated optical-resolution (OR) and acoustic-resolution (AR) multiscale PAM based on free-space light transmission and fast microelectromechanical systems (MEMS) scanning. The lateral resolution for OR is 4.9 µm, and the lateral resolution for AR is 114.5 µm. The maximum imaging depth for OR is 0.7 mm, and the maximum imaging depth for AR is 4.1 mm. The imaging speed can reach 50 k Alines per second. The high signal-to-noise ratios and wavelength throughput are achieved by delivering light via free-space, and the high speed is achieved by a MEMS scanning mirror. The blood vasculature from superficial skin to the deep tissue of a mouse leg was imaged
in vivo
using two different resolutions to demonstrate the multiscale imaging capability.</description><identifier>ISSN: 0146-9592</identifier><identifier>EISSN: 1539-4794</identifier><identifier>DOI: 10.1364/OL.397733</identifier><language>eng</language><publisher>Washington: Optical Society of America</publisher><subject>Acoustic noise ; Imaging ; Light transmission ; Microelectromechanical systems ; Microscopy ; Photoacoustic microscopy ; Scanning</subject><ispartof>Optics letters, 2020-08, Vol.45 (15), p.4312-4315</ispartof><rights>Copyright Optical Society of America Aug 1, 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c290t-efc8ce5c9a9feb6859be373559ae4bb22724bc37af099a590e7b0405f34d23813</citedby><cites>FETCH-LOGICAL-c290t-efc8ce5c9a9feb6859be373559ae4bb22724bc37af099a590e7b0405f34d23813</cites><orcidid>0000-0003-3668-3539 ; 0000-0001-7565-2044</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3245,27901,27902</link.rule.ids></links><search><creatorcontrib>Zhang, Chen</creatorcontrib><creatorcontrib>Zhao, Huangxuan</creatorcontrib><creatorcontrib>Xu, Song</creatorcontrib><creatorcontrib>Chen, Ningbo</creatorcontrib><creatorcontrib>Li, Ke</creatorcontrib><creatorcontrib>Jiang, Xinkuan</creatorcontrib><creatorcontrib>Liu, Liangjian</creatorcontrib><creatorcontrib>Liu, Zhicheng</creatorcontrib><creatorcontrib>Wang, Lidai</creatorcontrib><creatorcontrib>Wong, Kenneth K. Y.</creatorcontrib><creatorcontrib>Zou, Jun</creatorcontrib><creatorcontrib>Liu, Chengbo</creatorcontrib><creatorcontrib>Song, Liang</creatorcontrib><title>Multiscale high-speed photoacoustic microscopy based on free-space light transmission and a MEMS scanning mirror</title><title>Optics letters</title><description>The conventional photoacoustic microscopy (PAM) system allows trade-offs between lateral resolution and imaging depth, limiting its applications in biological imaging
in vivo
. Here we present an integrated optical-resolution (OR) and acoustic-resolution (AR) multiscale PAM based on free-space light transmission and fast microelectromechanical systems (MEMS) scanning. The lateral resolution for OR is 4.9 µm, and the lateral resolution for AR is 114.5 µm. The maximum imaging depth for OR is 0.7 mm, and the maximum imaging depth for AR is 4.1 mm. The imaging speed can reach 50 k Alines per second. The high signal-to-noise ratios and wavelength throughput are achieved by delivering light via free-space, and the high speed is achieved by a MEMS scanning mirror. The blood vasculature from superficial skin to the deep tissue of a mouse leg was imaged
in vivo
using two different resolutions to demonstrate the multiscale imaging capability.</description><subject>Acoustic noise</subject><subject>Imaging</subject><subject>Light transmission</subject><subject>Microelectromechanical systems</subject><subject>Microscopy</subject><subject>Photoacoustic microscopy</subject><subject>Scanning</subject><issn>0146-9592</issn><issn>1539-4794</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpdkDtPwzAUhS0EEqUw8A8sscCQ4rfjEVXlIaXqAMyR4960rtI42OnQf4-rMjHd4Xzn6NyD0D0lM8qVeF5VM2605vwCTajkphDaiEs0IVSowkjDrtFNSjtCiMrQBA3LQzf65GwHeOs32yINAGs8bMMYrAuHNHqH997FkFwYjrixKcuhx20EyLB1gLvsG_EYbZ_2PiWfVduvscXLxfIT5-y-9_0mp8QY4i26am2X4O7vTtH36-Jr_l5Uq7eP-UtVOGbIWEDrSgfSGWtaaFQpTQNccymNBdE0jGkmGse1bYkxVhoCuiGCyJaLNeMl5VP0eM4dYvg5QBrr3M1B19ke8ls1E8zoUileZvThH7oLh9jndpkShFKqmMrU05k6bZEitPUQ_d7GY01Jfdq-XlX1eXv-C3myd1Y</recordid><startdate>20200801</startdate><enddate>20200801</enddate><creator>Zhang, Chen</creator><creator>Zhao, Huangxuan</creator><creator>Xu, Song</creator><creator>Chen, Ningbo</creator><creator>Li, Ke</creator><creator>Jiang, Xinkuan</creator><creator>Liu, Liangjian</creator><creator>Liu, Zhicheng</creator><creator>Wang, Lidai</creator><creator>Wong, Kenneth K. Y.</creator><creator>Zou, Jun</creator><creator>Liu, Chengbo</creator><creator>Song, Liang</creator><general>Optical Society of America</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>H8D</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3668-3539</orcidid><orcidid>https://orcid.org/0000-0001-7565-2044</orcidid></search><sort><creationdate>20200801</creationdate><title>Multiscale high-speed photoacoustic microscopy based on free-space light transmission and a MEMS scanning mirror</title><author>Zhang, Chen ; Zhao, Huangxuan ; Xu, Song ; Chen, Ningbo ; Li, Ke ; Jiang, Xinkuan ; Liu, Liangjian ; Liu, Zhicheng ; Wang, Lidai ; Wong, Kenneth K. Y. ; Zou, Jun ; Liu, Chengbo ; Song, Liang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c290t-efc8ce5c9a9feb6859be373559ae4bb22724bc37af099a590e7b0405f34d23813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acoustic noise</topic><topic>Imaging</topic><topic>Light transmission</topic><topic>Microelectromechanical systems</topic><topic>Microscopy</topic><topic>Photoacoustic microscopy</topic><topic>Scanning</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Chen</creatorcontrib><creatorcontrib>Zhao, Huangxuan</creatorcontrib><creatorcontrib>Xu, Song</creatorcontrib><creatorcontrib>Chen, Ningbo</creatorcontrib><creatorcontrib>Li, Ke</creatorcontrib><creatorcontrib>Jiang, Xinkuan</creatorcontrib><creatorcontrib>Liu, Liangjian</creatorcontrib><creatorcontrib>Liu, Zhicheng</creatorcontrib><creatorcontrib>Wang, Lidai</creatorcontrib><creatorcontrib>Wong, Kenneth K. Y.</creatorcontrib><creatorcontrib>Zou, Jun</creatorcontrib><creatorcontrib>Liu, Chengbo</creatorcontrib><creatorcontrib>Song, Liang</creatorcontrib><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Aerospace Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Optics letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Chen</au><au>Zhao, Huangxuan</au><au>Xu, Song</au><au>Chen, Ningbo</au><au>Li, Ke</au><au>Jiang, Xinkuan</au><au>Liu, Liangjian</au><au>Liu, Zhicheng</au><au>Wang, Lidai</au><au>Wong, Kenneth K. Y.</au><au>Zou, Jun</au><au>Liu, Chengbo</au><au>Song, Liang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiscale high-speed photoacoustic microscopy based on free-space light transmission and a MEMS scanning mirror</atitle><jtitle>Optics letters</jtitle><date>2020-08-01</date><risdate>2020</risdate><volume>45</volume><issue>15</issue><spage>4312</spage><epage>4315</epage><pages>4312-4315</pages><issn>0146-9592</issn><eissn>1539-4794</eissn><abstract>The conventional photoacoustic microscopy (PAM) system allows trade-offs between lateral resolution and imaging depth, limiting its applications in biological imaging
in vivo
. Here we present an integrated optical-resolution (OR) and acoustic-resolution (AR) multiscale PAM based on free-space light transmission and fast microelectromechanical systems (MEMS) scanning. The lateral resolution for OR is 4.9 µm, and the lateral resolution for AR is 114.5 µm. The maximum imaging depth for OR is 0.7 mm, and the maximum imaging depth for AR is 4.1 mm. The imaging speed can reach 50 k Alines per second. The high signal-to-noise ratios and wavelength throughput are achieved by delivering light via free-space, and the high speed is achieved by a MEMS scanning mirror. The blood vasculature from superficial skin to the deep tissue of a mouse leg was imaged
in vivo
using two different resolutions to demonstrate the multiscale imaging capability.</abstract><cop>Washington</cop><pub>Optical Society of America</pub><doi>10.1364/OL.397733</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0003-3668-3539</orcidid><orcidid>https://orcid.org/0000-0001-7565-2044</orcidid></addata></record> |
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| ispartof | Optics letters, 2020-08, Vol.45 (15), p.4312-4315 |
| issn | 0146-9592 1539-4794 |
| language | eng |
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| source | Optica Publishing Group Journals |
| subjects | Acoustic noise Imaging Light transmission Microelectromechanical systems Microscopy Photoacoustic microscopy Scanning |
| title | Multiscale high-speed photoacoustic microscopy based on free-space light transmission and a MEMS scanning mirror |
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