Effect of Glycyrrhizic Acid and Arabinogalactan on the Membrane Potential of Rat Thymocytes Studied by Potential-Sensitive Fluorescent Probe
The effect of the natural saponin glycyrrhizic acid (GA) and polysaccharide arabinogalactan (AG) on the transmembrane potential of rat thymocytes was investigated using the potential-sensitive fluorescent probe 4-(p-dimethylaminostyryl)-1-methylpyridinium (DSM). Incubation of cells with GA in micell...
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| Veröffentlicht in: | The Journal of membrane biology 2020-08, Vol.253 (4), p.343-356 |
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| creator | Glazachev, Yuri I. Schlotgauer, Anna A. Timoshnikov, Viktor A. Kononova, Polina A. Selyutina, Olga Yu Shelepova, Ekaterina A. Zelikman, Maxim V. Khvostov, Mikhail V. Polyakov, Nikolay E. |
| description | The effect of the natural saponin glycyrrhizic acid (GA) and polysaccharide arabinogalactan (AG) on the transmembrane potential of rat thymocytes was investigated using the potential-sensitive fluorescent probe 4-(p-dimethylaminostyryl)-1-methylpyridinium (DSM). Incubation of cells with GA in micellar form resulted in a decrease of the amplitude of observed fluorescence kinetics that points out to a decrease of the transmembrane potential. The proposed mechanism is an increase of membrane ion permeability (passive ion transport) of the plasma cell membrane due to GA incorporation. The incorporation of GA molecules into the cell membrane is extremely sensitive to the degree of GA dissociation. The neutral form of glycyrrhizic acid enters the lipid bilayer in contrast to the deprotonated anionic form. The incubation of rat thymocytes with anionic form of GA, namely with its disodium salt, has no effect on the fluorescence kinetics. The possible reasons of this phenomenon are discussed in the light of the nuclear magnetic resonance (NMR) and molecular dynamics (MD) data. The treatment of thymocytes with AG affects only the initial rate of the probe incorporation. The proposed mechanism is that AG covers the surface of the cell membrane and forms a barrier for the probe. Additionally, our experiments demonstrated that both polysaccharide AG and GA in the neutral form (but not Na
2
GA) effectively capture the cationic probe in an aqueous solution and then deliver it to the cell membrane.
Graphic Abstract |
| doi_str_mv | 10.1007/s00232-020-00132-3 |
| format | Article |
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2
GA) effectively capture the cationic probe in an aqueous solution and then deliver it to the cell membrane.
Graphic Abstract</description><identifier>ISSN: 0022-2631</identifier><identifier>EISSN: 1432-1424</identifier><identifier>DOI: 10.1007/s00232-020-00132-3</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Aqueous solutions ; Arabinogalactan ; Biochemistry ; Biomedical and Life Sciences ; Cell membranes ; Cell surface ; Fluorescent indicators ; Human Physiology ; Incorporation ; Ion transport ; Kinetics ; Life Sciences ; Lipid bilayers ; Lipids ; Magnetic permeability ; Membrane permeability ; Membrane potential ; Molecular dynamics ; NMR ; Nuclear magnetic resonance ; Polysaccharides ; Saponins ; Thymocytes</subject><ispartof>The Journal of membrane biology, 2020-08, Vol.253 (4), p.343-356</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-c434b5e44c5818c413cc53f8702967b40a8f0abce086f9558b17923bca0ceb7d3</citedby><cites>FETCH-LOGICAL-c352t-c434b5e44c5818c413cc53f8702967b40a8f0abce086f9558b17923bca0ceb7d3</cites><orcidid>0000-0003-3686-7274</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00232-020-00132-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00232-020-00132-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Glazachev, Yuri I.</creatorcontrib><creatorcontrib>Schlotgauer, Anna A.</creatorcontrib><creatorcontrib>Timoshnikov, Viktor A.</creatorcontrib><creatorcontrib>Kononova, Polina A.</creatorcontrib><creatorcontrib>Selyutina, Olga Yu</creatorcontrib><creatorcontrib>Shelepova, Ekaterina A.</creatorcontrib><creatorcontrib>Zelikman, Maxim V.</creatorcontrib><creatorcontrib>Khvostov, Mikhail V.</creatorcontrib><creatorcontrib>Polyakov, Nikolay E.</creatorcontrib><title>Effect of Glycyrrhizic Acid and Arabinogalactan on the Membrane Potential of Rat Thymocytes Studied by Potential-Sensitive Fluorescent Probe</title><title>The Journal of membrane biology</title><addtitle>J Membrane Biol</addtitle><description>The effect of the natural saponin glycyrrhizic acid (GA) and polysaccharide arabinogalactan (AG) on the transmembrane potential of rat thymocytes was investigated using the potential-sensitive fluorescent probe 4-(p-dimethylaminostyryl)-1-methylpyridinium (DSM). Incubation of cells with GA in micellar form resulted in a decrease of the amplitude of observed fluorescence kinetics that points out to a decrease of the transmembrane potential. The proposed mechanism is an increase of membrane ion permeability (passive ion transport) of the plasma cell membrane due to GA incorporation. The incorporation of GA molecules into the cell membrane is extremely sensitive to the degree of GA dissociation. The neutral form of glycyrrhizic acid enters the lipid bilayer in contrast to the deprotonated anionic form. The incubation of rat thymocytes with anionic form of GA, namely with its disodium salt, has no effect on the fluorescence kinetics. The possible reasons of this phenomenon are discussed in the light of the nuclear magnetic resonance (NMR) and molecular dynamics (MD) data. The treatment of thymocytes with AG affects only the initial rate of the probe incorporation. The proposed mechanism is that AG covers the surface of the cell membrane and forms a barrier for the probe. Additionally, our experiments demonstrated that both polysaccharide AG and GA in the neutral form (but not Na
2
GA) effectively capture the cationic probe in an aqueous solution and then deliver it to the cell membrane.
Graphic Abstract</description><subject>Aqueous solutions</subject><subject>Arabinogalactan</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cell membranes</subject><subject>Cell surface</subject><subject>Fluorescent indicators</subject><subject>Human Physiology</subject><subject>Incorporation</subject><subject>Ion transport</subject><subject>Kinetics</subject><subject>Life Sciences</subject><subject>Lipid bilayers</subject><subject>Lipids</subject><subject>Magnetic permeability</subject><subject>Membrane permeability</subject><subject>Membrane potential</subject><subject>Molecular dynamics</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Polysaccharides</subject><subject>Saponins</subject><subject>Thymocytes</subject><issn>0022-2631</issn><issn>1432-1424</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1qHDEQhEVIIBs7L5CTIJdcxmn9zM7scTG2E3CIsZ2zkHp6vDKzkiNpDJNnyENbzgYMOeTUBf1V0U0x9kHAiQDoPmcAqWQDEhoAUZV6xVZCVyG01K_Zqu5lI9dKvGXvcr6vUNet9Yr9PhtHwsLjyC-mBZeUdv6XR75FP3AbBr5N1vkQ7-xksdjAY-BlR_wb7V2ygfhVLBSKt9NzxLUt_Ha37CMuhTK_KfPgaeBuecGaGwrZF_9I_HyaY6KMdcGvUnR0zN6Mdsr0_u88Yj_Oz25PvzSX3y--nm4vG1StLA1qpV1LWmPbix61UIitGvsO5GbdOQ22H8E6JOjX46Zteye6jVQOLSC5blBH7NMh9yHFnzPlYva-njFN9aE4ZyO17LXotdYV_fgPeh_nFOp1lVLQVhA2lZIHClPMOdFoHpLf27QYAea5IHMoyNSCzJ-CjKomdTDlCoc7Si_R_3E9AdfmlEc</recordid><startdate>20200801</startdate><enddate>20200801</enddate><creator>Glazachev, 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Glycyrrhizic Acid and Arabinogalactan on the Membrane Potential of Rat Thymocytes Studied by Potential-Sensitive Fluorescent Probe</title><author>Glazachev, Yuri I. ; Schlotgauer, Anna A. ; Timoshnikov, Viktor A. ; Kononova, Polina A. ; Selyutina, Olga Yu ; Shelepova, Ekaterina A. ; Zelikman, Maxim V. ; Khvostov, Mikhail V. ; Polyakov, Nikolay E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-c434b5e44c5818c413cc53f8702967b40a8f0abce086f9558b17923bca0ceb7d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aqueous solutions</topic><topic>Arabinogalactan</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cell membranes</topic><topic>Cell surface</topic><topic>Fluorescent indicators</topic><topic>Human Physiology</topic><topic>Incorporation</topic><topic>Ion transport</topic><topic>Kinetics</topic><topic>Life Sciences</topic><topic>Lipid bilayers</topic><topic>Lipids</topic><topic>Magnetic permeability</topic><topic>Membrane permeability</topic><topic>Membrane potential</topic><topic>Molecular dynamics</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Polysaccharides</topic><topic>Saponins</topic><topic>Thymocytes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Glazachev, Yuri I.</creatorcontrib><creatorcontrib>Schlotgauer, Anna A.</creatorcontrib><creatorcontrib>Timoshnikov, Viktor A.</creatorcontrib><creatorcontrib>Kononova, Polina A.</creatorcontrib><creatorcontrib>Selyutina, Olga Yu</creatorcontrib><creatorcontrib>Shelepova, Ekaterina A.</creatorcontrib><creatorcontrib>Zelikman, Maxim V.</creatorcontrib><creatorcontrib>Khvostov, Mikhail V.</creatorcontrib><creatorcontrib>Polyakov, Nikolay E.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & 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Probe</atitle><jtitle>The Journal of membrane biology</jtitle><stitle>J Membrane Biol</stitle><date>2020-08-01</date><risdate>2020</risdate><volume>253</volume><issue>4</issue><spage>343</spage><epage>356</epage><pages>343-356</pages><issn>0022-2631</issn><eissn>1432-1424</eissn><abstract>The effect of the natural saponin glycyrrhizic acid (GA) and polysaccharide arabinogalactan (AG) on the transmembrane potential of rat thymocytes was investigated using the potential-sensitive fluorescent probe 4-(p-dimethylaminostyryl)-1-methylpyridinium (DSM). Incubation of cells with GA in micellar form resulted in a decrease of the amplitude of observed fluorescence kinetics that points out to a decrease of the transmembrane potential. The proposed mechanism is an increase of membrane ion permeability (passive ion transport) of the plasma cell membrane due to GA incorporation. The incorporation of GA molecules into the cell membrane is extremely sensitive to the degree of GA dissociation. The neutral form of glycyrrhizic acid enters the lipid bilayer in contrast to the deprotonated anionic form. The incubation of rat thymocytes with anionic form of GA, namely with its disodium salt, has no effect on the fluorescence kinetics. The possible reasons of this phenomenon are discussed in the light of the nuclear magnetic resonance (NMR) and molecular dynamics (MD) data. The treatment of thymocytes with AG affects only the initial rate of the probe incorporation. The proposed mechanism is that AG covers the surface of the cell membrane and forms a barrier for the probe. Additionally, our experiments demonstrated that both polysaccharide AG and GA in the neutral form (but not Na
2
GA) effectively capture the cationic probe in an aqueous solution and then deliver it to the cell membrane.
Graphic Abstract</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s00232-020-00132-3</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0003-3686-7274</orcidid></addata></record> |
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| subjects | Aqueous solutions Arabinogalactan Biochemistry Biomedical and Life Sciences Cell membranes Cell surface Fluorescent indicators Human Physiology Incorporation Ion transport Kinetics Life Sciences Lipid bilayers Lipids Magnetic permeability Membrane permeability Membrane potential Molecular dynamics NMR Nuclear magnetic resonance Polysaccharides Saponins Thymocytes |
| title | Effect of Glycyrrhizic Acid and Arabinogalactan on the Membrane Potential of Rat Thymocytes Studied by Potential-Sensitive Fluorescent Probe |
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