Antioxidant Activity of Flavonoid Rich Fraction of Ligaria cuneifolia (Loranthaceae)
Ligaria cuneifolia (Ruiz & Pav.) Tiegh. (Loranthaceae), the ‘Argentine mistletoe’, is a hemiparasite species largely used in folk medicine. The aim of this study was to evaluate the antioxidant activity using in vitro, ex vivo, and in vivo methods. A screening of phenolics was performed by UV sp...
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| Veröffentlicht in: | Chemistry & biodiversity 2020-10, Vol.17 (10), p.e2000302-n/a |
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| creator | Dobrecky, Cecilia Marchini, Timoteo Ricco, Rafael Garcés, Mariana Gadano, Andrea Carballo, Marta Wagner, Marcelo Lucangioli, Silvia Evelson, Pablo |
| description | Ligaria cuneifolia (Ruiz & Pav.) Tiegh. (Loranthaceae), the ‘Argentine mistletoe’, is a hemiparasite species largely used in folk medicine. The aim of this study was to evaluate the antioxidant activity using in vitro, ex vivo, and in vivo methods. A screening of phenolics was performed by UV spectroscopy on different fractions. The antioxidant capacity was evaluated in vitro by the 1,1‐diphenyl‐2‐picrylhydrazyl radical (DPPH.) assay on a crude extract (CE), ethyl acetate fraction (EAF), and aqueous fraction (AF). The results suggest that EAF concentrates the antioxidant capacity and was selected for further analysis. Capillary electrophoresis was employed to monitor the individual antioxidant capacity and the potential contributors to this effect. Ex vivo assays showed an efficient inhibition of tert‐butyl hydroperoxide‐induced rat liver phospholipid oxidation, as well as rat brain autoxidation, and H2O2‐induced DNA damage in blood monocytes. In vivo, the topical application of EAF significantly decreased skin chemiluminescence in a mice model. |
| doi_str_mv | 10.1002/cbdv.202000302 |
| format | Article |
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Tiegh. (Loranthaceae), the ‘Argentine mistletoe’, is a hemiparasite species largely used in folk medicine. The aim of this study was to evaluate the antioxidant activity using in vitro, ex vivo, and in vivo methods. A screening of phenolics was performed by UV spectroscopy on different fractions. The antioxidant capacity was evaluated in vitro by the 1,1‐diphenyl‐2‐picrylhydrazyl radical (DPPH.) assay on a crude extract (CE), ethyl acetate fraction (EAF), and aqueous fraction (AF). The results suggest that EAF concentrates the antioxidant capacity and was selected for further analysis. Capillary electrophoresis was employed to monitor the individual antioxidant capacity and the potential contributors to this effect. Ex vivo assays showed an efficient inhibition of tert‐butyl hydroperoxide‐induced rat liver phospholipid oxidation, as well as rat brain autoxidation, and H2O2‐induced DNA damage in blood monocytes. In vivo, the topical application of EAF significantly decreased skin chemiluminescence in a mice model.</description><identifier>ISSN: 1612-1872</identifier><identifier>EISSN: 1612-1880</identifier><identifier>DOI: 10.1002/cbdv.202000302</identifier><identifier>PMID: 32725761</identifier><language>eng</language><publisher>Switzerland: Wiley Subscription Services, Inc</publisher><subject><![CDATA[Acetic acid ; Animals ; antioxidant activity ; Antioxidants ; Antioxidants - chemistry ; Antioxidants - isolation & purification ; Antioxidants - pharmacology ; Argentina ; Autoxidation ; Biphenyl Compounds - antagonists & inhibitors ; Brain damage ; Butyl hydroperoxide ; Capillary electrophoresis ; Chemiluminescence ; DNA Damage ; Electrophoresis ; Ethyl acetate ; Female ; flavonoid ; Flavonoids ; Flavonoids - chemistry ; Flavonoids - isolation & purification ; Flavonoids - pharmacology ; Hydrogen peroxide ; In vivo methods and tests ; Liver - drug effects ; Liver - metabolism ; Loranthaceae ; Loranthaceae - chemistry ; Mice ; Monocytes ; Oxidation ; Oxidation-Reduction ; Oxidative stress ; Parasitic plants ; Phenols ; Phospholipids ; Phospholipids - antagonists & inhibitors ; Phospholipids - metabolism ; Picrates - antagonists & inhibitors ; Plant Extracts - chemistry ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; quercetin ; Rats ; Rats, Sprague-Dawley ; Spectroscopy ; tert-Butylhydroperoxide - antagonists & inhibitors ; tert-Butylhydroperoxide - pharmacology ; Topical application]]></subject><ispartof>Chemistry & biodiversity, 2020-10, Vol.17 (10), p.e2000302-n/a</ispartof><rights>2020 Wiley‐VHCA AG, Zurich, Switzerland</rights><rights>2020 Wiley-VHCA AG, Zurich, Switzerland.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3282-dea28e9aeda4f7e35a5931d2f0f699735f3149027528ebe3744a48f7c4c59a4d3</citedby><cites>FETCH-LOGICAL-c3282-dea28e9aeda4f7e35a5931d2f0f699735f3149027528ebe3744a48f7c4c59a4d3</cites><orcidid>0000-0002-6575-0246</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbdv.202000302$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbdv.202000302$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27926,27927,45576,45577</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32725761$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dobrecky, Cecilia</creatorcontrib><creatorcontrib>Marchini, Timoteo</creatorcontrib><creatorcontrib>Ricco, Rafael</creatorcontrib><creatorcontrib>Garcés, Mariana</creatorcontrib><creatorcontrib>Gadano, Andrea</creatorcontrib><creatorcontrib>Carballo, Marta</creatorcontrib><creatorcontrib>Wagner, Marcelo</creatorcontrib><creatorcontrib>Lucangioli, Silvia</creatorcontrib><creatorcontrib>Evelson, Pablo</creatorcontrib><title>Antioxidant Activity of Flavonoid Rich Fraction of Ligaria cuneifolia (Loranthaceae)</title><title>Chemistry & biodiversity</title><addtitle>Chem Biodivers</addtitle><description>Ligaria cuneifolia (Ruiz & Pav.) Tiegh. (Loranthaceae), the ‘Argentine mistletoe’, is a hemiparasite species largely used in folk medicine. The aim of this study was to evaluate the antioxidant activity using in vitro, ex vivo, and in vivo methods. A screening of phenolics was performed by UV spectroscopy on different fractions. The antioxidant capacity was evaluated in vitro by the 1,1‐diphenyl‐2‐picrylhydrazyl radical (DPPH.) assay on a crude extract (CE), ethyl acetate fraction (EAF), and aqueous fraction (AF). The results suggest that EAF concentrates the antioxidant capacity and was selected for further analysis. Capillary electrophoresis was employed to monitor the individual antioxidant capacity and the potential contributors to this effect. Ex vivo assays showed an efficient inhibition of tert‐butyl hydroperoxide‐induced rat liver phospholipid oxidation, as well as rat brain autoxidation, and H2O2‐induced DNA damage in blood monocytes. In vivo, the topical application of EAF significantly decreased skin chemiluminescence in a mice model.</description><subject>Acetic acid</subject><subject>Animals</subject><subject>antioxidant activity</subject><subject>Antioxidants</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - isolation & purification</subject><subject>Antioxidants - pharmacology</subject><subject>Argentina</subject><subject>Autoxidation</subject><subject>Biphenyl Compounds - antagonists & inhibitors</subject><subject>Brain damage</subject><subject>Butyl hydroperoxide</subject><subject>Capillary electrophoresis</subject><subject>Chemiluminescence</subject><subject>DNA Damage</subject><subject>Electrophoresis</subject><subject>Ethyl acetate</subject><subject>Female</subject><subject>flavonoid</subject><subject>Flavonoids</subject><subject>Flavonoids - chemistry</subject><subject>Flavonoids - isolation & purification</subject><subject>Flavonoids - pharmacology</subject><subject>Hydrogen peroxide</subject><subject>In vivo methods and tests</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Loranthaceae</subject><subject>Loranthaceae - chemistry</subject><subject>Mice</subject><subject>Monocytes</subject><subject>Oxidation</subject><subject>Oxidation-Reduction</subject><subject>Oxidative stress</subject><subject>Parasitic plants</subject><subject>Phenols</subject><subject>Phospholipids</subject><subject>Phospholipids - antagonists & inhibitors</subject><subject>Phospholipids - metabolism</subject><subject>Picrates - antagonists & inhibitors</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>quercetin</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Spectroscopy</subject><subject>tert-Butylhydroperoxide - antagonists & inhibitors</subject><subject>tert-Butylhydroperoxide - pharmacology</subject><subject>Topical application</subject><issn>1612-1872</issn><issn>1612-1880</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0M1LwzAYBvAgipsfV49S8DIPnflqkxzndCoMBJleQ5YmLqNrZtpO99-bsTnBi6e85P3lITwAXCDYRxDiGz0tVn0MMYSQQHwAuihHOEWcw8P9zHAHnNT1PPp4z49Bh2CGM5ajLpgMqsb5L1eoqkkGunEr16wTb5NRqVa-8q5IXpyeJaOg4tJXm9XYvavgVKLbyjjryzj2xj7EhJnSRpnrM3BkVVmb8915Cl5H95PhYzp-fngaDsapJpjjtDAKcyOUKRS1zJBMZYKgAltocyEYySxBVEDMssimhjBKFeWWaaozoWhBTkFvm7sM_qM1dSMXrtamLFVlfFtLTDGniEOBIr36Q-e-DVX8XVQZzQkVhEXV3yodfF0HY-UyuIUKa4mg3PQtN33Lfd_xweUutp0uTLHnPwVHILbg05Vm_U-cHN7evf2GfwNkPori</recordid><startdate>202010</startdate><enddate>202010</enddate><creator>Dobrecky, Cecilia</creator><creator>Marchini, Timoteo</creator><creator>Ricco, Rafael</creator><creator>Garcés, Mariana</creator><creator>Gadano, Andrea</creator><creator>Carballo, Marta</creator><creator>Wagner, Marcelo</creator><creator>Lucangioli, Silvia</creator><creator>Evelson, Pablo</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6575-0246</orcidid></search><sort><creationdate>202010</creationdate><title>Antioxidant Activity of Flavonoid Rich Fraction of Ligaria cuneifolia (Loranthaceae)</title><author>Dobrecky, Cecilia ; Marchini, Timoteo ; Ricco, Rafael ; Garcés, Mariana ; Gadano, Andrea ; Carballo, Marta ; Wagner, Marcelo ; Lucangioli, Silvia ; Evelson, Pablo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3282-dea28e9aeda4f7e35a5931d2f0f699735f3149027528ebe3744a48f7c4c59a4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acetic acid</topic><topic>Animals</topic><topic>antioxidant activity</topic><topic>Antioxidants</topic><topic>Antioxidants - chemistry</topic><topic>Antioxidants - isolation & purification</topic><topic>Antioxidants - pharmacology</topic><topic>Argentina</topic><topic>Autoxidation</topic><topic>Biphenyl Compounds - antagonists & inhibitors</topic><topic>Brain damage</topic><topic>Butyl hydroperoxide</topic><topic>Capillary electrophoresis</topic><topic>Chemiluminescence</topic><topic>DNA Damage</topic><topic>Electrophoresis</topic><topic>Ethyl acetate</topic><topic>Female</topic><topic>flavonoid</topic><topic>Flavonoids</topic><topic>Flavonoids - chemistry</topic><topic>Flavonoids - isolation & purification</topic><topic>Flavonoids - pharmacology</topic><topic>Hydrogen peroxide</topic><topic>In vivo methods and tests</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Loranthaceae</topic><topic>Loranthaceae - chemistry</topic><topic>Mice</topic><topic>Monocytes</topic><topic>Oxidation</topic><topic>Oxidation-Reduction</topic><topic>Oxidative stress</topic><topic>Parasitic plants</topic><topic>Phenols</topic><topic>Phospholipids</topic><topic>Phospholipids - antagonists & inhibitors</topic><topic>Phospholipids - metabolism</topic><topic>Picrates - antagonists & inhibitors</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>quercetin</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Spectroscopy</topic><topic>tert-Butylhydroperoxide - antagonists & inhibitors</topic><topic>tert-Butylhydroperoxide - pharmacology</topic><topic>Topical application</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dobrecky, Cecilia</creatorcontrib><creatorcontrib>Marchini, Timoteo</creatorcontrib><creatorcontrib>Ricco, Rafael</creatorcontrib><creatorcontrib>Garcés, Mariana</creatorcontrib><creatorcontrib>Gadano, Andrea</creatorcontrib><creatorcontrib>Carballo, Marta</creatorcontrib><creatorcontrib>Wagner, Marcelo</creatorcontrib><creatorcontrib>Lucangioli, Silvia</creatorcontrib><creatorcontrib>Evelson, Pablo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chemistry & biodiversity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dobrecky, Cecilia</au><au>Marchini, Timoteo</au><au>Ricco, Rafael</au><au>Garcés, Mariana</au><au>Gadano, Andrea</au><au>Carballo, Marta</au><au>Wagner, Marcelo</au><au>Lucangioli, Silvia</au><au>Evelson, Pablo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antioxidant Activity of Flavonoid Rich Fraction of Ligaria cuneifolia (Loranthaceae)</atitle><jtitle>Chemistry & biodiversity</jtitle><addtitle>Chem Biodivers</addtitle><date>2020-10</date><risdate>2020</risdate><volume>17</volume><issue>10</issue><spage>e2000302</spage><epage>n/a</epage><pages>e2000302-n/a</pages><issn>1612-1872</issn><eissn>1612-1880</eissn><abstract>Ligaria cuneifolia (Ruiz & Pav.) Tiegh. (Loranthaceae), the ‘Argentine mistletoe’, is a hemiparasite species largely used in folk medicine. The aim of this study was to evaluate the antioxidant activity using in vitro, ex vivo, and in vivo methods. A screening of phenolics was performed by UV spectroscopy on different fractions. The antioxidant capacity was evaluated in vitro by the 1,1‐diphenyl‐2‐picrylhydrazyl radical (DPPH.) assay on a crude extract (CE), ethyl acetate fraction (EAF), and aqueous fraction (AF). The results suggest that EAF concentrates the antioxidant capacity and was selected for further analysis. Capillary electrophoresis was employed to monitor the individual antioxidant capacity and the potential contributors to this effect. Ex vivo assays showed an efficient inhibition of tert‐butyl hydroperoxide‐induced rat liver phospholipid oxidation, as well as rat brain autoxidation, and H2O2‐induced DNA damage in blood monocytes. In vivo, the topical application of EAF significantly decreased skin chemiluminescence in a mice model.</abstract><cop>Switzerland</cop><pub>Wiley Subscription Services, Inc</pub><pmid>32725761</pmid><doi>10.1002/cbdv.202000302</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-6575-0246</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acetic acid Animals antioxidant activity Antioxidants Antioxidants - chemistry Antioxidants - isolation & purification Antioxidants - pharmacology Argentina Autoxidation Biphenyl Compounds - antagonists & inhibitors Brain damage Butyl hydroperoxide Capillary electrophoresis Chemiluminescence DNA Damage Electrophoresis Ethyl acetate Female flavonoid Flavonoids Flavonoids - chemistry Flavonoids - isolation & purification Flavonoids - pharmacology Hydrogen peroxide In vivo methods and tests Liver - drug effects Liver - metabolism Loranthaceae Loranthaceae - chemistry Mice Monocytes Oxidation Oxidation-Reduction Oxidative stress Parasitic plants Phenols Phospholipids Phospholipids - antagonists & inhibitors Phospholipids - metabolism Picrates - antagonists & inhibitors Plant Extracts - chemistry Plant Extracts - isolation & purification Plant Extracts - pharmacology quercetin Rats Rats, Sprague-Dawley Spectroscopy tert-Butylhydroperoxide - antagonists & inhibitors tert-Butylhydroperoxide - pharmacology Topical application |
| title | Antioxidant Activity of Flavonoid Rich Fraction of Ligaria cuneifolia (Loranthaceae) |
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