Efficient allele conversion in mouse zygotes and primary cells based on electroporation of Cre protein

•Cre-loxP recombination can be mediated via Cre protein electroporation.•Electroporation of Cre protein is efficient in mouse zygotes and primary cells.•Cre protein electroporation can be combined with zygotes obtained from IVF.•Cre protein electroporation has no toxic effects on electroporated zygo...

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Veröffentlicht in:	Methods (San Diego, Calif.) Calif.), 2021-07, Vol.191, p.87-94
Hauptverfasser:	Jenickova, Irena, Kasparek, Petr, Petrezselyova, Silvia, Elias, Jan, Prochazka, Jan, Kopkanova, Jana, Navratil, Michal, Barinka, Cyril, Sedlacek, Radislav
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Animals Conditional Delivery Electroporation Integrases - genetics loxP MEF Mice Recombination Transgenesis Zygote
Online-Zugang:	Volltext
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