Genome-wide identification and characterization of nonspecific lipid transfer protein (nsLTP) genes in Arachis duranensis

Nonspecific lipid transfer proteins (nsLTPs) play vital roles in lipid metabolism, cell apoptosis and biotic and abiotic stresses in plants. However, the distribution of nsLTPs in Arachis duranensis has not been fully characterized. In this study, we identified 64 nsLTP genes in A. duranensis (designated AdLTPs), which were classified into six subfamilies and randomly distributed along nine chromosomes. Tandem and segmental duplication events were detected in the evolution of AdLTPs. The Ks and ω values differed significantly between Types 1 and D subfamilies, and eight AdLTPs were under positive selection. The expression levels of AdLTPs were changed after salinity, PEG, low-temperature and ABA treatments. Three AdLTPs were associated with resistance to nematode infection, and DOF and WRI1 transcription factors may regulate the AdLTP response to nematode infection. Our results may provide valuable genomic information for the breeding of peanut cultivars that are resistant to biotic and abiotic stresses. •AdLTPs were classified into 6 subfamilies and were randomly distributed along 9 chromosomes.•The tandem and segmental duplication events were detected in the evolution of AdLTPs.•The Ks and ω values were significantly different between Type 1 and D subfamilies.•The expression level of AdLTPs was changed after salinity, PEG, low temperature and ABA treatments.•DOF and WRI1 transcription factors may regulate the AdLTP response to nematode infection.