Gas‐phase hydration of the lysozyme ion produced by infrared‐laser ablation of a droplet beam studied by photodissociation and fluorescence spectroscopy
Biomolecules function in an aqueous environment. Elucidation of the hydration structures of biomolecules is hence important to understand their functions. Here, we investigated the hydration structure of lysozyme (Lys) in the gas phase by photodissociation and fluorescence spectroscopy in combinatio...
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| Veröffentlicht in: | Journal of mass spectrometry. 2021-04, Vol.56 (4), p.e4620-n/a |
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| container_title | Journal of mass spectrometry. |
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| creator | Asami, Hiroya Kawauchi, Norishi Kohno, Jun‐ya |
| description | Biomolecules function in an aqueous environment. Elucidation of the hydration structures of biomolecules is hence important to understand their functions. Here, we investigated the hydration structure of lysozyme (Lys) in the gas phase by photodissociation and fluorescence spectroscopy in combination with droplet‐beam laser ablation mass spectrometry. We found that water molecules are held inside and on the surface of the Lys molecule, and the hydration structure around the tryptophan residue changes by photoexcitation. This study provides a novel method to observe the hydration structures of large biomolecules at the molecular level. |
| doi_str_mv | 10.1002/jms.4620 |
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Elucidation of the hydration structures of biomolecules is hence important to understand their functions. Here, we investigated the hydration structure of lysozyme (Lys) in the gas phase by photodissociation and fluorescence spectroscopy in combination with droplet‐beam laser ablation mass spectrometry. We found that water molecules are held inside and on the surface of the Lys molecule, and the hydration structure around the tryptophan residue changes by photoexcitation. This study provides a novel method to observe the hydration structures of large biomolecules at the molecular level.</description><identifier>ISSN: 1076-5174</identifier><identifier>EISSN: 1096-9888</identifier><identifier>DOI: 10.1002/jms.4620</identifier><identifier>PMID: 32721078</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Ablation ; Analytical methods ; Aqueous environments ; Biomolecules ; droplet beam ; Droplets ; Fluorescence ; Fluorescence spectroscopy ; Hydration ; Infrared lasers ; IR‐laser ablation ; Laser ablation ; Laser beams ; Lasers ; Lysozyme ; Mass spectrometry ; Mass spectroscopy ; Molecular structure ; Photodissociation ; Photoexcitation ; Spectroscopy ; spectroscopyfluorescence spectroscopy ; Tryptophan ; Vapor phases ; Water chemistry</subject><ispartof>Journal of mass spectrometry., 2021-04, Vol.56 (4), p.e4620-n/a</ispartof><rights>2020 John Wiley & Sons, Ltd.</rights><rights>2021 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4150-24c3e4796479a95c04e079c041d4ccd512465b398a4e7edbbfe7edb3727321643</citedby><cites>FETCH-LOGICAL-c4150-24c3e4796479a95c04e079c041d4ccd512465b398a4e7edbbfe7edb3727321643</cites><orcidid>0000-0001-6239-9289</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjms.4620$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjms.4620$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32721078$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Asami, Hiroya</creatorcontrib><creatorcontrib>Kawauchi, Norishi</creatorcontrib><creatorcontrib>Kohno, Jun‐ya</creatorcontrib><title>Gas‐phase hydration of the lysozyme ion produced by infrared‐laser ablation of a droplet beam studied by photodissociation and fluorescence spectroscopy</title><title>Journal of mass spectrometry.</title><addtitle>J Mass Spectrom</addtitle><description>Biomolecules function in an aqueous environment. Elucidation of the hydration structures of biomolecules is hence important to understand their functions. Here, we investigated the hydration structure of lysozyme (Lys) in the gas phase by photodissociation and fluorescence spectroscopy in combination with droplet‐beam laser ablation mass spectrometry. We found that water molecules are held inside and on the surface of the Lys molecule, and the hydration structure around the tryptophan residue changes by photoexcitation. This study provides a novel method to observe the hydration structures of large biomolecules at the molecular level.</description><subject>Ablation</subject><subject>Analytical methods</subject><subject>Aqueous environments</subject><subject>Biomolecules</subject><subject>droplet beam</subject><subject>Droplets</subject><subject>Fluorescence</subject><subject>Fluorescence spectroscopy</subject><subject>Hydration</subject><subject>Infrared lasers</subject><subject>IR‐laser ablation</subject><subject>Laser ablation</subject><subject>Laser beams</subject><subject>Lasers</subject><subject>Lysozyme</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Molecular structure</subject><subject>Photodissociation</subject><subject>Photoexcitation</subject><subject>Spectroscopy</subject><subject>spectroscopyfluorescence spectroscopy</subject><subject>Tryptophan</subject><subject>Vapor phases</subject><subject>Water chemistry</subject><issn>1076-5174</issn><issn>1096-9888</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kc1K7TAUhYMo_oNPIAEnd1JN0jRthyJ6VBQH6jikyS6nh7SpSYvUkY9wH-A-3X0Sczz-gOAg7LDzrcXeWQgdUHJMCWEnizYcc8HIGtqmpBRJWRTF-vKeiySjOd9COyEsCCFlycUm2kpZzuJjsY3-zVT4__q3n6sAeD4Zr4bGddjVeJgDtlNwL1MLeNnrvTOjBoOrCTdd7ZUHE6U2Kj1Wlf1SKmy86y0MuALV4jCMplnJ-rkbnGlCcLpZ4aozuLaj8xA0dBpw6EEP3gXt-mkPbdTKBtj_qLvo8eL84ewyubmbXZ2d3iSa04wkjOsUeF6KeFSZacKB5GUs1HCtTUYZF1mVloXikIOpqvq9pDnLU0YFT3fRn5VvXPFphDDItonjWKs6cGOQjLOCCEJFEdGjH-jCjb6L00mWUR7_nWbZt6GOmwQPtex90yo_SUrkMjEZE5PLxCJ6-GE4Vi2YL_AzoggkK-C5sTD9aiSvb-_fDd8AZmOkCw</recordid><startdate>202104</startdate><enddate>202104</enddate><creator>Asami, Hiroya</creator><creator>Kawauchi, Norishi</creator><creator>Kohno, Jun‐ya</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H97</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L.G</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6239-9289</orcidid></search><sort><creationdate>202104</creationdate><title>Gas‐phase hydration of the lysozyme ion produced by infrared‐laser ablation of a droplet beam studied by photodissociation and fluorescence spectroscopy</title><author>Asami, Hiroya ; Kawauchi, Norishi ; Kohno, Jun‐ya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4150-24c3e4796479a95c04e079c041d4ccd512465b398a4e7edbbfe7edb3727321643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Ablation</topic><topic>Analytical methods</topic><topic>Aqueous environments</topic><topic>Biomolecules</topic><topic>droplet beam</topic><topic>Droplets</topic><topic>Fluorescence</topic><topic>Fluorescence spectroscopy</topic><topic>Hydration</topic><topic>Infrared lasers</topic><topic>IR‐laser ablation</topic><topic>Laser ablation</topic><topic>Laser beams</topic><topic>Lasers</topic><topic>Lysozyme</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Molecular structure</topic><topic>Photodissociation</topic><topic>Photoexcitation</topic><topic>Spectroscopy</topic><topic>spectroscopyfluorescence spectroscopy</topic><topic>Tryptophan</topic><topic>Vapor phases</topic><topic>Water chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Asami, Hiroya</creatorcontrib><creatorcontrib>Kawauchi, Norishi</creatorcontrib><creatorcontrib>Kohno, Jun‐ya</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of mass spectrometry.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Asami, Hiroya</au><au>Kawauchi, Norishi</au><au>Kohno, Jun‐ya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gas‐phase hydration of the lysozyme ion produced by infrared‐laser ablation of a droplet beam studied by photodissociation and fluorescence spectroscopy</atitle><jtitle>Journal of mass spectrometry.</jtitle><addtitle>J Mass Spectrom</addtitle><date>2021-04</date><risdate>2021</risdate><volume>56</volume><issue>4</issue><spage>e4620</spage><epage>n/a</epage><pages>e4620-n/a</pages><issn>1076-5174</issn><eissn>1096-9888</eissn><abstract>Biomolecules function in an aqueous environment. Elucidation of the hydration structures of biomolecules is hence important to understand their functions. Here, we investigated the hydration structure of lysozyme (Lys) in the gas phase by photodissociation and fluorescence spectroscopy in combination with droplet‐beam laser ablation mass spectrometry. We found that water molecules are held inside and on the surface of the Lys molecule, and the hydration structure around the tryptophan residue changes by photoexcitation. This study provides a novel method to observe the hydration structures of large biomolecules at the molecular level.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>32721078</pmid><doi>10.1002/jms.4620</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-6239-9289</orcidid></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Ablation Analytical methods Aqueous environments Biomolecules droplet beam Droplets Fluorescence Fluorescence spectroscopy Hydration Infrared lasers IR‐laser ablation Laser ablation Laser beams Lasers Lysozyme Mass spectrometry Mass spectroscopy Molecular structure Photodissociation Photoexcitation Spectroscopy spectroscopyfluorescence spectroscopy Tryptophan Vapor phases Water chemistry |
| title | Gas‐phase hydration of the lysozyme ion produced by infrared‐laser ablation of a droplet beam studied by photodissociation and fluorescence spectroscopy |
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