Screening and characterization of an α-L-fucosidase from Bacteroides fragilis NCTC9343 for synthesis of fucosyl-N-acetylglucosamine disaccharides

Fucosyl- N -acetylglucosamine disaccharides are present in many biologically important oligosaccharides, such as human milk oligosaccharides, Lewis carbohydrate antigens, and glycans on cell-surface glycoconjugate receptors, and thus have vast potential for infant formulas, prebiotics, and pharmaceu...

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Veröffentlicht in:	Applied microbiology and biotechnology 2020-09, Vol.104 (18), p.7827-7840
Hauptverfasser:	Liu, Peng, Zhang, Huaqin, Wang, Yuying, Chen, Xiaodi, Jin, Lan, Xu, Li, Xiao, Min
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylglucosamine alpha-L-Fucosidase - genetics alpha-L-Fucosidase - metabolism Antigens Bacteroides fragilis Bacteroides fragilis - genetics Bacteroides fragilis - metabolism Binding sites Biocatalysts Biomedical and Life Sciences Biotechnologically Relevant Enzymes and Proteins Biotechnology Breast milk Carbohydrates Cell surface Computer simulation Disaccharides Enzymatic synthesis Enzymes Glycoproteins Glycosidases Humans Infant formulas Laboratories Life Sciences Microbial Genetics and Genomics Microbiology Milk Molecular dynamics N-Acetylglucosamine Oligosaccharides pH effects Phylogenetics Phylogeny Polysaccharides Prebiotics Reaction time Sequence analysis Simulation analysis Substrate Specificity Substrates Synthesis Thermal simulation Three dimensional models
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