Exposure to Moderate Glycosuria Induces Virulence of Group B Streptococcus
Abstract To explore whether glycosuria induces virulence of uropathogens, in turn facilitating urinary tract infection (UTI), we exposed group B Streptococcus (GBS) strain 10/84 to human urine plain or with 300 mg/dL glucose (mimicking moderate glycosuria). Exposure to moderate glycosuria significan...
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| Veröffentlicht in: | The Journal of infectious diseases 2021-03, Vol.223 (5), p.843-847 |
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| container_issue | 5 |
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| container_title | The Journal of infectious diseases |
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| creator | John, Preeti P Baker, Brady C Paudel, Santosh Nassour, Lauren Cagle, Hayden Kulkarni, Ritwij |
| description | Abstract
To explore whether glycosuria induces virulence of uropathogens, in turn facilitating urinary tract infection (UTI), we exposed group B Streptococcus (GBS) strain 10/84 to human urine plain or with 300 mg/dL glucose (mimicking moderate glycosuria). Exposure to moderate glycosuria significantly augmented bacterial growth, kidney bacterial burden in a mouse model of ascending UTI, and virulence characteristics and expression of corresponding genes. Exposure to glycosuria increased GBS adherence to human bladder epithelial cell line and expression of corresponding PI2a fimbrial gene, antimicrobial peptide LL-37 resistance and bacterial surface charge modulating dltA, and GBS hemolytic ability and expression of genes encoding pore-forming toxins. |
| doi_str_mv | 10.1093/infdis/jiaa443 |
| format | Article |
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To explore whether glycosuria induces virulence of uropathogens, in turn facilitating urinary tract infection (UTI), we exposed group B Streptococcus (GBS) strain 10/84 to human urine plain or with 300 mg/dL glucose (mimicking moderate glycosuria). Exposure to moderate glycosuria significantly augmented bacterial growth, kidney bacterial burden in a mouse model of ascending UTI, and virulence characteristics and expression of corresponding genes. Exposure to glycosuria increased GBS adherence to human bladder epithelial cell line and expression of corresponding PI2a fimbrial gene, antimicrobial peptide LL-37 resistance and bacterial surface charge modulating dltA, and GBS hemolytic ability and expression of genes encoding pore-forming toxins.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/jiaa443</identifier><identifier>PMID: 32702082</identifier><language>eng</language><publisher>US: Oxford University Press</publisher><subject>Animals ; Antimicrobial Peptides ; Bacteria ; Bacterial Adhesion ; Cell Line ; Diabetes ; Disease transmission ; Epithelial cells ; Glycosuria - complications ; Humans ; Kidneys ; Mice ; Mimicry ; Pathogens ; Streptococcal Infections - microbiology ; Streptococcus ; Streptococcus agalactiae - genetics ; Streptococcus agalactiae - pathogenicity ; Streptococcus infections ; Surface charge ; Urinary tract ; Urinary tract infections ; Urinary Tract Infections - microbiology ; Virulence</subject><ispartof>The Journal of infectious diseases, 2021-03, Vol.223 (5), p.843-847</ispartof><rights>The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com. 2020</rights><rights>The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-e06d8afa2fb3372b7c8fcc8e9a31f63285de11a0fac49e943204ab4a210ff0ad3</citedby><cites>FETCH-LOGICAL-c357t-e06d8afa2fb3372b7c8fcc8e9a31f63285de11a0fac49e943204ab4a210ff0ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32702082$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>John, Preeti P</creatorcontrib><creatorcontrib>Baker, Brady C</creatorcontrib><creatorcontrib>Paudel, Santosh</creatorcontrib><creatorcontrib>Nassour, Lauren</creatorcontrib><creatorcontrib>Cagle, Hayden</creatorcontrib><creatorcontrib>Kulkarni, Ritwij</creatorcontrib><title>Exposure to Moderate Glycosuria Induces Virulence of Group B Streptococcus</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>Abstract
To explore whether glycosuria induces virulence of uropathogens, in turn facilitating urinary tract infection (UTI), we exposed group B Streptococcus (GBS) strain 10/84 to human urine plain or with 300 mg/dL glucose (mimicking moderate glycosuria). Exposure to moderate glycosuria significantly augmented bacterial growth, kidney bacterial burden in a mouse model of ascending UTI, and virulence characteristics and expression of corresponding genes. 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Baker, Brady C ; Paudel, Santosh ; Nassour, Lauren ; Cagle, Hayden ; Kulkarni, Ritwij</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-e06d8afa2fb3372b7c8fcc8e9a31f63285de11a0fac49e943204ab4a210ff0ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Antimicrobial Peptides</topic><topic>Bacteria</topic><topic>Bacterial Adhesion</topic><topic>Cell Line</topic><topic>Diabetes</topic><topic>Disease transmission</topic><topic>Epithelial cells</topic><topic>Glycosuria - complications</topic><topic>Humans</topic><topic>Kidneys</topic><topic>Mice</topic><topic>Mimicry</topic><topic>Pathogens</topic><topic>Streptococcal Infections - microbiology</topic><topic>Streptococcus</topic><topic>Streptococcus agalactiae - genetics</topic><topic>Streptococcus agalactiae - pathogenicity</topic><topic>Streptococcus infections</topic><topic>Surface charge</topic><topic>Urinary tract</topic><topic>Urinary tract infections</topic><topic>Urinary Tract Infections - microbiology</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>John, Preeti P</creatorcontrib><creatorcontrib>Baker, Brady C</creatorcontrib><creatorcontrib>Paudel, Santosh</creatorcontrib><creatorcontrib>Nassour, Lauren</creatorcontrib><creatorcontrib>Cagle, Hayden</creatorcontrib><creatorcontrib>Kulkarni, Ritwij</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>John, Preeti P</au><au>Baker, Brady C</au><au>Paudel, Santosh</au><au>Nassour, Lauren</au><au>Cagle, Hayden</au><au>Kulkarni, Ritwij</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exposure to Moderate Glycosuria Induces Virulence of Group B Streptococcus</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>2021-03-03</date><risdate>2021</risdate><volume>223</volume><issue>5</issue><spage>843</spage><epage>847</epage><pages>843-847</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><abstract>Abstract
To explore whether glycosuria induces virulence of uropathogens, in turn facilitating urinary tract infection (UTI), we exposed group B Streptococcus (GBS) strain 10/84 to human urine plain or with 300 mg/dL glucose (mimicking moderate glycosuria). Exposure to moderate glycosuria significantly augmented bacterial growth, kidney bacterial burden in a mouse model of ascending UTI, and virulence characteristics and expression of corresponding genes. Exposure to glycosuria increased GBS adherence to human bladder epithelial cell line and expression of corresponding PI2a fimbrial gene, antimicrobial peptide LL-37 resistance and bacterial surface charge modulating dltA, and GBS hemolytic ability and expression of genes encoding pore-forming toxins.</abstract><cop>US</cop><pub>Oxford University Press</pub><pmid>32702082</pmid><doi>10.1093/infdis/jiaa443</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Animals Antimicrobial Peptides Bacteria Bacterial Adhesion Cell Line Diabetes Disease transmission Epithelial cells Glycosuria - complications Humans Kidneys Mice Mimicry Pathogens Streptococcal Infections - microbiology Streptococcus Streptococcus agalactiae - genetics Streptococcus agalactiae - pathogenicity Streptococcus infections Surface charge Urinary tract Urinary tract infections Urinary Tract Infections - microbiology Virulence |
| title | Exposure to Moderate Glycosuria Induces Virulence of Group B Streptococcus |
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