Description of Maribellus sediminis sp. nov., a marine nitrogen-fixing bacterium isolated from sediment of cordgrass and mangrove
Two marine bacterial strains designated Y2-1-60T and GM1-28 were isolated from sediments of cordgrass and mangrove along the Luoyang estuary in Quanzhou Bay, China, respectively. Both strains were Gram-staining-negative, straight rod-shaped, non-flagellum, facultatively anaerobic, nitrogen-fixing, a...
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| Veröffentlicht in: | Systematic and applied microbiology 2020-07, Vol.43 (4), p.126099-126099, Article 126099 |
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| description | Two marine bacterial strains designated Y2-1-60T and GM1-28 were isolated from sediments of cordgrass and mangrove along the Luoyang estuary in Quanzhou Bay, China, respectively. Both strains were Gram-staining-negative, straight rod-shaped, non-flagellum, facultatively anaerobic, nitrogen-fixing, and did not contain carotenoid pigment. Catalase activities were found to be weak positive and oxidase activities negative. The 16S rRNA gene sequences of the two strains were identical and had maximum similarity of 98.0% with Maribellus luteus XSD2T, and of 10%) consisted of iso-C15:0, and iso-C17:0 3-OH. The polar lipids consisted of phosphatidylethanolamine and several unidentified lipids. The genomic G+C contents were 41.9–42.0mol%. Gene annotation revealed that strains Y2-1-60T and GM1-28 contained a set of nif gene cluster (nifHDKENB) responsible for nitrogen fixation. Based on the above characteristics, strains Y2-1-60T and GM1-28 represent a novel species within the genus Maribellus. Thus, Maribellus sediminis sp. nov. is proposed with type strain Y2-1-60T (=MCCC 1K04285T=KCTC 72884T), isolated from cordgrass sediment and strain GM1-28 (=MCCC 1K04384=KCTC 72880), isolated from mangrove sediment. |
| doi_str_mv | 10.1016/j.syapm.2020.126099 |
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Both strains were Gram-staining-negative, straight rod-shaped, non-flagellum, facultatively anaerobic, nitrogen-fixing, and did not contain carotenoid pigment. Catalase activities were found to be weak positive and oxidase activities negative. The 16S rRNA gene sequences of the two strains were identical and had maximum similarity of 98.0% with Maribellus luteus XSD2T, and of <94.5% with other species. ANI value (96.9%) and DDH estimate (71.5%) between the two strains supported that they belonged to the same species. ANI value and DDH estimate between the two strains and M. luteus XSD2T was 74.3% and 19.4%, respectively, indicating that they represent a novel species. Phylogenetic analysis based on 16S rRNA gene and phylogenomic analysis indicated that strains Y2-1-60T and GM1-28 formed a monophyletic branch within the genus Maribellus. The respiratory quinone was menaquinone MK-7. The major fatty acid (>10%) consisted of iso-C15:0, and iso-C17:0 3-OH. The polar lipids consisted of phosphatidylethanolamine and several unidentified lipids. The genomic G+C contents were 41.9–42.0mol%. Gene annotation revealed that strains Y2-1-60T and GM1-28 contained a set of nif gene cluster (nifHDKENB) responsible for nitrogen fixation. Based on the above characteristics, strains Y2-1-60T and GM1-28 represent a novel species within the genus Maribellus. Thus, Maribellus sediminis sp. nov. is proposed with type strain Y2-1-60T (=MCCC 1K04285T=KCTC 72884T), isolated from cordgrass sediment and strain GM1-28 (=MCCC 1K04384=KCTC 72880), isolated from mangrove sediment.</description><identifier>ISSN: 0723-2020</identifier><identifier>EISSN: 1618-0984</identifier><identifier>DOI: 10.1016/j.syapm.2020.126099</identifier><identifier>PMID: 32690193</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Bacteroidetes - chemistry ; Bacteroidetes - classification ; Bacteroidetes - cytology ; Bacteroidetes - physiology ; China ; Coastal sediment ; Cordgrass ; DNA, Bacterial - genetics ; Estuaries ; Fatty Acids - analysis ; Genes, Bacterial - genetics ; Genome, Bacterial - genetics ; Geologic Sediments - microbiology ; Mangrove ; Maribellus ; Nitrogen - metabolism ; Nucleic Acid Hybridization ; Phospholipids - analysis ; Phylogeny ; Poaceae - microbiology ; Polyphasic taxonomy ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Vitamin K 2 - analogs & derivatives ; Vitamin K 2 - analysis</subject><ispartof>Systematic and applied microbiology, 2020-07, Vol.43 (4), p.126099-126099, Article 126099</ispartof><rights>2020 Elsevier GmbH</rights><rights>Copyright © 2020 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-add8482d01fc0d04d6983f537dc8b5d6e6f45f25303bf71d14669242e8e1205f3</citedby><cites>FETCH-LOGICAL-c359t-add8482d01fc0d04d6983f537dc8b5d6e6f45f25303bf71d14669242e8e1205f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.syapm.2020.126099$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32690193$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Zhaobin</creatorcontrib><creatorcontrib>Hu, Yuzhong</creatorcontrib><creatorcontrib>Lai, Qiliang</creatorcontrib><creatorcontrib>Guo, Yu</creatorcontrib><title>Description of Maribellus sediminis sp. nov., a marine nitrogen-fixing bacterium isolated from sediment of cordgrass and mangrove</title><title>Systematic and applied microbiology</title><addtitle>Syst Appl Microbiol</addtitle><description>Two marine bacterial strains designated Y2-1-60T and GM1-28 were isolated from sediments of cordgrass and mangrove along the Luoyang estuary in Quanzhou Bay, China, respectively. Both strains were Gram-staining-negative, straight rod-shaped, non-flagellum, facultatively anaerobic, nitrogen-fixing, and did not contain carotenoid pigment. Catalase activities were found to be weak positive and oxidase activities negative. The 16S rRNA gene sequences of the two strains were identical and had maximum similarity of 98.0% with Maribellus luteus XSD2T, and of <94.5% with other species. ANI value (96.9%) and DDH estimate (71.5%) between the two strains supported that they belonged to the same species. ANI value and DDH estimate between the two strains and M. luteus XSD2T was 74.3% and 19.4%, respectively, indicating that they represent a novel species. Phylogenetic analysis based on 16S rRNA gene and phylogenomic analysis indicated that strains Y2-1-60T and GM1-28 formed a monophyletic branch within the genus Maribellus. The respiratory quinone was menaquinone MK-7. The major fatty acid (>10%) consisted of iso-C15:0, and iso-C17:0 3-OH. The polar lipids consisted of phosphatidylethanolamine and several unidentified lipids. The genomic G+C contents were 41.9–42.0mol%. Gene annotation revealed that strains Y2-1-60T and GM1-28 contained a set of nif gene cluster (nifHDKENB) responsible for nitrogen fixation. Based on the above characteristics, strains Y2-1-60T and GM1-28 represent a novel species within the genus Maribellus. Thus, Maribellus sediminis sp. nov. is proposed with type strain Y2-1-60T (=MCCC 1K04285T=KCTC 72884T), isolated from cordgrass sediment and strain GM1-28 (=MCCC 1K04384=KCTC 72880), isolated from mangrove sediment.</description><subject>Bacteroidetes - chemistry</subject><subject>Bacteroidetes - classification</subject><subject>Bacteroidetes - cytology</subject><subject>Bacteroidetes - physiology</subject><subject>China</subject><subject>Coastal sediment</subject><subject>Cordgrass</subject><subject>DNA, Bacterial - genetics</subject><subject>Estuaries</subject><subject>Fatty Acids - analysis</subject><subject>Genes, Bacterial - genetics</subject><subject>Genome, Bacterial - genetics</subject><subject>Geologic Sediments - microbiology</subject><subject>Mangrove</subject><subject>Maribellus</subject><subject>Nitrogen - metabolism</subject><subject>Nucleic Acid Hybridization</subject><subject>Phospholipids - analysis</subject><subject>Phylogeny</subject><subject>Poaceae - microbiology</subject><subject>Polyphasic taxonomy</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Vitamin K 2 - analogs & derivatives</subject><subject>Vitamin K 2 - analysis</subject><issn>0723-2020</issn><issn>1618-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1u3CAUhVGVqJmkfYJKFcssYocfmzGLLqr8VkrVTbtGGC4jRmNwwR4ly7x5mDjNsisQfPdcnQ-hL5TUlFBxua3zkx6HmhFWXpggUn5AKypoVxHZNUdoRdaMV4fvE3Sa85YQ2khBP6ITzoQkVPIVer6GbJIfJx8Djg7_1Mn3sNvNGWewfvDBl9tY4xD39QXWeChAABz8lOIGQuX8ow8b3GszQfLzgH2OOz2BxS7FYQmBMB2yTUx2k3TOWAdbgsImxT18QsdO7zJ8fjvP0J_bm99X99XDr7sfV98fKsNbOVXa2q7pmCXUGWJJY4XsuGv52pqub60A4ZrWsZYT3rs1tbQRQrKGQQeUkdbxM3S-5I4p_p0hT2rw2ZSqOkCcsypsW6RwygrKF9SkmHMCp8bkS_EnRYk6uFdb9epeHeSqxX2Z-vq2YO4HsO8z_2QX4NsCQKm595BUNh6CKYoSmEnZ6P-74AUe55fw</recordid><startdate>202007</startdate><enddate>202007</enddate><creator>Huang, Zhaobin</creator><creator>Hu, Yuzhong</creator><creator>Lai, Qiliang</creator><creator>Guo, Yu</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202007</creationdate><title>Description of Maribellus sediminis sp. nov., a marine nitrogen-fixing bacterium isolated from sediment of cordgrass and mangrove</title><author>Huang, Zhaobin ; Hu, Yuzhong ; Lai, Qiliang ; Guo, Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-add8482d01fc0d04d6983f537dc8b5d6e6f45f25303bf71d14669242e8e1205f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Bacteroidetes - chemistry</topic><topic>Bacteroidetes - classification</topic><topic>Bacteroidetes - cytology</topic><topic>Bacteroidetes - physiology</topic><topic>China</topic><topic>Coastal sediment</topic><topic>Cordgrass</topic><topic>DNA, Bacterial - genetics</topic><topic>Estuaries</topic><topic>Fatty Acids - analysis</topic><topic>Genes, Bacterial - genetics</topic><topic>Genome, Bacterial - genetics</topic><topic>Geologic Sediments - microbiology</topic><topic>Mangrove</topic><topic>Maribellus</topic><topic>Nitrogen - metabolism</topic><topic>Nucleic Acid Hybridization</topic><topic>Phospholipids - analysis</topic><topic>Phylogeny</topic><topic>Poaceae - microbiology</topic><topic>Polyphasic taxonomy</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Vitamin K 2 - analogs & derivatives</topic><topic>Vitamin K 2 - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Zhaobin</creatorcontrib><creatorcontrib>Hu, Yuzhong</creatorcontrib><creatorcontrib>Lai, Qiliang</creatorcontrib><creatorcontrib>Guo, Yu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Systematic and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Zhaobin</au><au>Hu, Yuzhong</au><au>Lai, Qiliang</au><au>Guo, Yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Description of Maribellus sediminis sp. nov., a marine nitrogen-fixing bacterium isolated from sediment of cordgrass and mangrove</atitle><jtitle>Systematic and applied microbiology</jtitle><addtitle>Syst Appl Microbiol</addtitle><date>2020-07</date><risdate>2020</risdate><volume>43</volume><issue>4</issue><spage>126099</spage><epage>126099</epage><pages>126099-126099</pages><artnum>126099</artnum><issn>0723-2020</issn><eissn>1618-0984</eissn><abstract>Two marine bacterial strains designated Y2-1-60T and GM1-28 were isolated from sediments of cordgrass and mangrove along the Luoyang estuary in Quanzhou Bay, China, respectively. Both strains were Gram-staining-negative, straight rod-shaped, non-flagellum, facultatively anaerobic, nitrogen-fixing, and did not contain carotenoid pigment. Catalase activities were found to be weak positive and oxidase activities negative. The 16S rRNA gene sequences of the two strains were identical and had maximum similarity of 98.0% with Maribellus luteus XSD2T, and of <94.5% with other species. ANI value (96.9%) and DDH estimate (71.5%) between the two strains supported that they belonged to the same species. ANI value and DDH estimate between the two strains and M. luteus XSD2T was 74.3% and 19.4%, respectively, indicating that they represent a novel species. Phylogenetic analysis based on 16S rRNA gene and phylogenomic analysis indicated that strains Y2-1-60T and GM1-28 formed a monophyletic branch within the genus Maribellus. The respiratory quinone was menaquinone MK-7. The major fatty acid (>10%) consisted of iso-C15:0, and iso-C17:0 3-OH. The polar lipids consisted of phosphatidylethanolamine and several unidentified lipids. The genomic G+C contents were 41.9–42.0mol%. Gene annotation revealed that strains Y2-1-60T and GM1-28 contained a set of nif gene cluster (nifHDKENB) responsible for nitrogen fixation. Based on the above characteristics, strains Y2-1-60T and GM1-28 represent a novel species within the genus Maribellus. Thus, Maribellus sediminis sp. nov. is proposed with type strain Y2-1-60T (=MCCC 1K04285T=KCTC 72884T), isolated from cordgrass sediment and strain GM1-28 (=MCCC 1K04384=KCTC 72880), isolated from mangrove sediment.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>32690193</pmid><doi>10.1016/j.syapm.2020.126099</doi><tpages>1</tpages></addata></record> |
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| ispartof | Systematic and applied microbiology, 2020-07, Vol.43 (4), p.126099-126099, Article 126099 |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Bacteroidetes - chemistry Bacteroidetes - classification Bacteroidetes - cytology Bacteroidetes - physiology China Coastal sediment Cordgrass DNA, Bacterial - genetics Estuaries Fatty Acids - analysis Genes, Bacterial - genetics Genome, Bacterial - genetics Geologic Sediments - microbiology Mangrove Maribellus Nitrogen - metabolism Nucleic Acid Hybridization Phospholipids - analysis Phylogeny Poaceae - microbiology Polyphasic taxonomy RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Vitamin K 2 - analogs & derivatives Vitamin K 2 - analysis |
| title | Description of Maribellus sediminis sp. nov., a marine nitrogen-fixing bacterium isolated from sediment of cordgrass and mangrove |
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