Picoliter enzyme reactor on a nanofluidic device exceeding the bulk reaction rate

Single-cell analyses have recently become important to understand cell heterogeneity, the mechanism of cell function, and diseases. In contrast to single-cell analyses that target nucleic acids, single-cell protein analyses still pose challenges. We have proposed a general concept of integration and...

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Veröffentlicht in:	Analyst (London) 2020-08, Vol.145 (17), p.581-587
Hauptverfasser:	Yamamoto, Koki, Morikawa, Kyojiro, Imanaka, Hiroyuki, Imamura, Koreyoshi, Kitamori, Takehiko
Format:	Artikel
Sprache:	eng
Schlagworte:	Bioreactors Digestion Enzymes Enzymes, Immobilized Fluidics Heterogeneity Immobilization Nanochannels Nanofluids Nucleic acids Proteins Proteomics Reaction time Shotguns Trypsin
Online-Zugang:	Volltext
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