Unexpected Anomeric Acceptor Preference Observed Using dDNP NMR for Transglycosylation Studies of β‑Galactosidases

The transglycosylation abilities of β-galactosidases were investigated using hyperpolarized [U–13C,U–2H]­glucose as an acceptor and o-nitrophenyl β-galactopyranoside as a donor. Several products were readily observable, and at least in the case when O3 acted as an acceptor, the enzymes showed a clea...

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Veröffentlicht in:	Biochemistry (Easton) 2020-08, Vol.59 (31), p.2903-2908
Hauptverfasser:	Kjeldsen, Christian, Ardenkjær-Larsen, Jan H, Duus, Jens. Ø
Format:	Artikel
Sprache:	eng
Schlagworte:	beta-Galactosidase - metabolism Galactose - chemistry Galactose - metabolism Glucose - chemistry Glucose - metabolism Glycosylation Kinetics Nuclear Magnetic Resonance, Biomolecular Stereoisomerism Substrate Specificity
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