Improved immobilization of lipase from Thermomyces lanuginosus on a new chitosan-based heterofunctional support: Mixed ion exchange plus hydrophobic interactions

In this study, a new mixed heterofunctional support (Chit–GA–Gly) has been prepared by sequential activation of chitosan hydrogel (Chit) with glutaraldehyde (GA) and further functionalization with glycine (Gly). The immobilization of the lipase from Thermomyces lanuginosus (TLL) on this support was...

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Veröffentlicht in:	International journal of biological macromolecules 2020-11, Vol.163, p.550-561
Hauptverfasser:	Okura, Nicole S., Sabi, Guilherme J., Crivellenti, Marcela C., Gomes, Raphael A.B., Fernandez-Lafuente, Roberto, Mendes, Adriano A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Biocatalysis Catalytic properties Chemical Phenomena Chitosan - chemistry Chitosan-based hydrogels Enzymes, Immobilized Eurotiales - enzymology Heterofunctional supports Hydrogen-Ion Concentration Hydrolysis Hydrophobic and Hydrophilic Interactions Lipase Lipase - chemistry Solvents Spectroscopy, Fourier Transform Infrared Thermogravimetry
Online-Zugang:	Volltext
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