Sigh maneuver protects healthy lungs during mechanical ventilation in adult Wistar rats

Mechanical ventilation (MV) is a tool used for the treatment of patients with acute or chronic respiratory failure. However, MV is a non-physiological resource, and it can cause metabolic disorders such as release of pro-inflammatory cytokines and production of reactive oxygen species. In clinical s...

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Veröffentlicht in:Experimental biology and medicine (Maywood, N.J.) N.J.), 2020-09, Vol.245 (15), p.1404-1413
Hauptverfasser: da Silva, Andréa Cristiane Lopes, de Matos, Natália Alves, de Souza, Ana Beatriz Farias, Castro, Thalles de Freitas, Cândido, Leandro da Silva, Oliveira, Michel Angelo das Graças Silva, Costa, Guilherme de Paula, Talvani, André, Cangussú, Sílvia Dantas, Bezerra, Frank Silva
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container_end_page 1413
container_issue 15
container_start_page 1404
container_title Experimental biology and medicine (Maywood, N.J.)
container_volume 245
creator da Silva, Andréa Cristiane Lopes
de Matos, Natália Alves
de Souza, Ana Beatriz Farias
Castro, Thalles de Freitas
Cândido, Leandro da Silva
Oliveira, Michel Angelo das Graças Silva
Costa, Guilherme de Paula
Talvani, André
Cangussú, Sílvia Dantas
Bezerra, Frank Silva
description Mechanical ventilation (MV) is a tool used for the treatment of patients with acute or chronic respiratory failure. However, MV is a non-physiological resource, and it can cause metabolic disorders such as release of pro-inflammatory cytokines and production of reactive oxygen species. In clinical setting, maneuvers such as sigh, are used to protect the lungs. Thus, this study aimed to evaluate the effects of sigh on oxidative stress and lung inflammation in healthy adult Wistar rats submitted to MV. Male Wistar rats were divided into four groups: control (CG), mechanical ventilation (MVG), MV set at 20 sighs/h (MVG20), and MV set at 40 sighs/h (MVG40). The MVG, MVG20, and MVG40 were submitted to MV for 1 h. After the protocol, all animals were euthanized and the blood, bronchoalveolar lavage fluid, and lungs were collected for subsequent analysis. In the arterial blood, MVG40 presented higher partial pressure of oxygen and lower partial pressure of carbon dioxide compared to control. The levels of bicarbonate in MVG20 were lower compared to CG. The neutrophil influx in bronchoalveolar lavage fluid was higher in the MVG compared to CG and MVG40. In the lung parenchyma, the lipid peroxidation was higher in MVG compared to CG, MVG20, and MVG40. Superoxide dismutase and catalase activity were higher in MVG compared to CG, MVG20, and MVG40. The levels of IL-1, IL-6, and TNF in the lung homogenate were higher in MVG compared to CG, MVG20, and MVG40. The use of sigh plays a protective role as it reduced redox imbalance and pulmonary inflammation caused by MV.
doi_str_mv 10.1177/1535370220940995
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However, MV is a non-physiological resource, and it can cause metabolic disorders such as release of pro-inflammatory cytokines and production of reactive oxygen species. In clinical setting, maneuvers such as sigh, are used to protect the lungs. Thus, this study aimed to evaluate the effects of sigh on oxidative stress and lung inflammation in healthy adult Wistar rats submitted to MV. Male Wistar rats were divided into four groups: control (CG), mechanical ventilation (MVG), MV set at 20 sighs/h (MVG20), and MV set at 40 sighs/h (MVG40). The MVG, MVG20, and MVG40 were submitted to MV for 1 h. After the protocol, all animals were euthanized and the blood, bronchoalveolar lavage fluid, and lungs were collected for subsequent analysis. In the arterial blood, MVG40 presented higher partial pressure of oxygen and lower partial pressure of carbon dioxide compared to control. The levels of bicarbonate in MVG20 were lower compared to CG. The neutrophil influx in bronchoalveolar lavage fluid was higher in the MVG compared to CG and MVG40. In the lung parenchyma, the lipid peroxidation was higher in MVG compared to CG, MVG20, and MVG40. Superoxide dismutase and catalase activity were higher in MVG compared to CG, MVG20, and MVG40. The levels of IL-1, IL-6, and TNF in the lung homogenate were higher in MVG compared to CG, MVG20, and MVG40. The use of sigh plays a protective role as it reduced redox imbalance and pulmonary inflammation caused by MV.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>32640895</pmid><doi>10.1177/1535370220940995</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-0804-5196</orcidid><orcidid>https://orcid.org/0000-0002-1061-6445</orcidid><oa>free_for_read</oa></addata></record>
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subjects Aging - pathology
Animals
Biomarkers - metabolism
Blood Gas Analysis
Bronchoalveolar Lavage Fluid - cytology
Hemodynamics
Inflammation Mediators - metabolism
Lung - pathology
Lung - physiopathology
Male
Original Research
Oxidative Stress
Rats, Wistar
Respiration, Artificial
Respiratory Function Tests
title Sigh maneuver protects healthy lungs during mechanical ventilation in adult Wistar rats
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