Sigh maneuver protects healthy lungs during mechanical ventilation in adult Wistar rats
Mechanical ventilation (MV) is a tool used for the treatment of patients with acute or chronic respiratory failure. However, MV is a non-physiological resource, and it can cause metabolic disorders such as release of pro-inflammatory cytokines and production of reactive oxygen species. In clinical s...
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creator | da Silva, Andréa Cristiane Lopes de Matos, Natália Alves de Souza, Ana Beatriz Farias Castro, Thalles de Freitas Cândido, Leandro da Silva Oliveira, Michel Angelo das Graças Silva Costa, Guilherme de Paula Talvani, André Cangussú, Sílvia Dantas Bezerra, Frank Silva |
description | Mechanical ventilation (MV) is a tool used for the treatment of patients with acute or chronic respiratory failure. However, MV is a non-physiological resource, and it can cause metabolic disorders such as release of pro-inflammatory cytokines and production of reactive oxygen species. In clinical setting, maneuvers such as sigh, are used to protect the lungs. Thus, this study aimed to evaluate the effects of sigh on oxidative stress and lung inflammation in healthy adult Wistar rats submitted to MV. Male Wistar rats were divided into four groups: control (CG), mechanical ventilation (MVG), MV set at 20 sighs/h (MVG20), and MV set at 40 sighs/h (MVG40). The MVG, MVG20, and MVG40 were submitted to MV for 1 h. After the protocol, all animals were euthanized and the blood, bronchoalveolar lavage fluid, and lungs were collected for subsequent analysis. In the arterial blood, MVG40 presented higher partial pressure of oxygen and lower partial pressure of carbon dioxide compared to control. The levels of bicarbonate in MVG20 were lower compared to CG. The neutrophil influx in bronchoalveolar lavage fluid was higher in the MVG compared to CG and MVG40. In the lung parenchyma, the lipid peroxidation was higher in MVG compared to CG, MVG20, and MVG40. Superoxide dismutase and catalase activity were higher in MVG compared to CG, MVG20, and MVG40. The levels of IL-1, IL-6, and TNF in the lung homogenate were higher in MVG compared to CG, MVG20, and MVG40. The use of sigh plays a protective role as it reduced redox imbalance and pulmonary inflammation caused by MV. |
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However, MV is a non-physiological resource, and it can cause metabolic disorders such as release of pro-inflammatory cytokines and production of reactive oxygen species. In clinical setting, maneuvers such as sigh, are used to protect the lungs. Thus, this study aimed to evaluate the effects of sigh on oxidative stress and lung inflammation in healthy adult Wistar rats submitted to MV. Male Wistar rats were divided into four groups: control (CG), mechanical ventilation (MVG), MV set at 20 sighs/h (MVG20), and MV set at 40 sighs/h (MVG40). The MVG, MVG20, and MVG40 were submitted to MV for 1 h. After the protocol, all animals were euthanized and the blood, bronchoalveolar lavage fluid, and lungs were collected for subsequent analysis. In the arterial blood, MVG40 presented higher partial pressure of oxygen and lower partial pressure of carbon dioxide compared to control. The levels of bicarbonate in MVG20 were lower compared to CG. The neutrophil influx in bronchoalveolar lavage fluid was higher in the MVG compared to CG and MVG40. In the lung parenchyma, the lipid peroxidation was higher in MVG compared to CG, MVG20, and MVG40. Superoxide dismutase and catalase activity were higher in MVG compared to CG, MVG20, and MVG40. The levels of IL-1, IL-6, and TNF in the lung homogenate were higher in MVG compared to CG, MVG20, and MVG40. The use of sigh plays a protective role as it reduced redox imbalance and pulmonary inflammation caused by MV.</description><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>DOI: 10.1177/1535370220940995</identifier><identifier>PMID: 32640895</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Aging - pathology ; Animals ; Biomarkers - metabolism ; Blood Gas Analysis ; Bronchoalveolar Lavage Fluid - cytology ; Hemodynamics ; Inflammation Mediators - metabolism ; Lung - pathology ; Lung - physiopathology ; Male ; Original Research ; Oxidative Stress ; Rats, Wistar ; Respiration, Artificial ; Respiratory Function Tests</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 2020-09, Vol.245 (15), p.1404-1413</ispartof><rights>2020 by the Society for Experimental Biology and Medicine</rights><rights>2020 by the Society for Experimental Biology and Medicine 2020 The Society for Experimental Biology and Medicine</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-514e61fc385a83282642358065c0664741a34d5f752a1a1919eeef7b513aca783</citedby><cites>FETCH-LOGICAL-c434t-514e61fc385a83282642358065c0664741a34d5f752a1a1919eeef7b513aca783</cites><orcidid>0000-0002-0804-5196 ; 0000-0002-1061-6445</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7441342/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7441342/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,21817,27922,27923,43619,43620,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32640895$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>da Silva, Andréa Cristiane Lopes</creatorcontrib><creatorcontrib>de Matos, Natália Alves</creatorcontrib><creatorcontrib>de Souza, Ana Beatriz Farias</creatorcontrib><creatorcontrib>Castro, Thalles de Freitas</creatorcontrib><creatorcontrib>Cândido, Leandro da Silva</creatorcontrib><creatorcontrib>Oliveira, Michel Angelo das Graças Silva</creatorcontrib><creatorcontrib>Costa, Guilherme de Paula</creatorcontrib><creatorcontrib>Talvani, André</creatorcontrib><creatorcontrib>Cangussú, Sílvia Dantas</creatorcontrib><creatorcontrib>Bezerra, Frank Silva</creatorcontrib><title>Sigh maneuver protects healthy lungs during mechanical ventilation in adult Wistar rats</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Exp Biol Med (Maywood)</addtitle><description>Mechanical ventilation (MV) is a tool used for the treatment of patients with acute or chronic respiratory failure. However, MV is a non-physiological resource, and it can cause metabolic disorders such as release of pro-inflammatory cytokines and production of reactive oxygen species. In clinical setting, maneuvers such as sigh, are used to protect the lungs. Thus, this study aimed to evaluate the effects of sigh on oxidative stress and lung inflammation in healthy adult Wistar rats submitted to MV. Male Wistar rats were divided into four groups: control (CG), mechanical ventilation (MVG), MV set at 20 sighs/h (MVG20), and MV set at 40 sighs/h (MVG40). The MVG, MVG20, and MVG40 were submitted to MV for 1 h. After the protocol, all animals were euthanized and the blood, bronchoalveolar lavage fluid, and lungs were collected for subsequent analysis. In the arterial blood, MVG40 presented higher partial pressure of oxygen and lower partial pressure of carbon dioxide compared to control. The levels of bicarbonate in MVG20 were lower compared to CG. The neutrophil influx in bronchoalveolar lavage fluid was higher in the MVG compared to CG and MVG40. In the lung parenchyma, the lipid peroxidation was higher in MVG compared to CG, MVG20, and MVG40. Superoxide dismutase and catalase activity were higher in MVG compared to CG, MVG20, and MVG40. The levels of IL-1, IL-6, and TNF in the lung homogenate were higher in MVG compared to CG, MVG20, and MVG40. The use of sigh plays a protective role as it reduced redox imbalance and pulmonary inflammation caused by MV.</description><subject>Aging - pathology</subject><subject>Animals</subject><subject>Biomarkers - metabolism</subject><subject>Blood Gas Analysis</subject><subject>Bronchoalveolar Lavage Fluid - cytology</subject><subject>Hemodynamics</subject><subject>Inflammation Mediators - metabolism</subject><subject>Lung - pathology</subject><subject>Lung - physiopathology</subject><subject>Male</subject><subject>Original Research</subject><subject>Oxidative Stress</subject><subject>Rats, Wistar</subject><subject>Respiration, Artificial</subject><subject>Respiratory Function Tests</subject><issn>1535-3702</issn><issn>1535-3699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UcFKJDEQDYuy6rj3PUmOXlqTTtLpXBYWcVUQPKh4DGUm3R1Jp8ckPTB_v5FRUcFTFVWvXj3eQ-g3JSeUSnlKBRNMkromihOlxA-0_zKqWKPUzltf9nvoIKUnQqiQdfMT7bG64aRVYh893Lp-wCMEO69txKs4ZWtywoMFn4cN9nPoE17O0YUej9YMEJwBj9c2ZOchuylgFzAsZ5_xg0sZIo6Q0yHa7cAn--u1LtD9v_O7s8vq-ubi6uzvdWU447kSlNuGdoa1AlpWt0VXzURLGmFI03DJKTC-FJ0UNVCgiiprbScfBWVgQLZsgf5seVfz42iXpsiK4PUquhHiRk_g9OdNcIPup7WWnFNWni3Q8StBnJ5nm7IeXTLW-2LJNCdd8-IuUS2lBUq2UBOnlKLt3t9Qol_y0F_zKCdHH-W9H7wFUADVFpCgt_ppmmModn1P-B9sL5Np</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>da Silva, Andréa Cristiane Lopes</creator><creator>de Matos, Natália Alves</creator><creator>de Souza, Ana Beatriz Farias</creator><creator>Castro, Thalles de Freitas</creator><creator>Cândido, Leandro da Silva</creator><creator>Oliveira, Michel Angelo das Graças Silva</creator><creator>Costa, Guilherme de Paula</creator><creator>Talvani, André</creator><creator>Cangussú, Sílvia Dantas</creator><creator>Bezerra, Frank Silva</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0804-5196</orcidid><orcidid>https://orcid.org/0000-0002-1061-6445</orcidid></search><sort><creationdate>20200901</creationdate><title>Sigh maneuver protects healthy lungs during mechanical ventilation in adult Wistar rats</title><author>da Silva, Andréa Cristiane Lopes ; de Matos, Natália Alves ; de Souza, Ana Beatriz Farias ; Castro, Thalles de Freitas ; Cândido, Leandro da Silva ; Oliveira, Michel Angelo das Graças Silva ; Costa, Guilherme de Paula ; Talvani, André ; Cangussú, Sílvia Dantas ; Bezerra, Frank Silva</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-514e61fc385a83282642358065c0664741a34d5f752a1a1919eeef7b513aca783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aging - pathology</topic><topic>Animals</topic><topic>Biomarkers - metabolism</topic><topic>Blood Gas Analysis</topic><topic>Bronchoalveolar Lavage Fluid - cytology</topic><topic>Hemodynamics</topic><topic>Inflammation Mediators - metabolism</topic><topic>Lung - pathology</topic><topic>Lung - physiopathology</topic><topic>Male</topic><topic>Original Research</topic><topic>Oxidative Stress</topic><topic>Rats, Wistar</topic><topic>Respiration, Artificial</topic><topic>Respiratory Function Tests</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>da Silva, Andréa Cristiane Lopes</creatorcontrib><creatorcontrib>de Matos, Natália Alves</creatorcontrib><creatorcontrib>de Souza, Ana Beatriz Farias</creatorcontrib><creatorcontrib>Castro, Thalles de Freitas</creatorcontrib><creatorcontrib>Cândido, Leandro da Silva</creatorcontrib><creatorcontrib>Oliveira, Michel Angelo das Graças Silva</creatorcontrib><creatorcontrib>Costa, Guilherme de Paula</creatorcontrib><creatorcontrib>Talvani, André</creatorcontrib><creatorcontrib>Cangussú, Sílvia Dantas</creatorcontrib><creatorcontrib>Bezerra, Frank Silva</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>da Silva, Andréa Cristiane Lopes</au><au>de Matos, Natália Alves</au><au>de Souza, Ana Beatriz Farias</au><au>Castro, Thalles de Freitas</au><au>Cândido, Leandro da Silva</au><au>Oliveira, Michel Angelo das Graças Silva</au><au>Costa, Guilherme de Paula</au><au>Talvani, André</au><au>Cangussú, Sílvia Dantas</au><au>Bezerra, Frank Silva</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sigh maneuver protects healthy lungs during mechanical ventilation in adult Wistar rats</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Exp Biol Med (Maywood)</addtitle><date>2020-09-01</date><risdate>2020</risdate><volume>245</volume><issue>15</issue><spage>1404</spage><epage>1413</epage><pages>1404-1413</pages><issn>1535-3702</issn><eissn>1535-3699</eissn><abstract>Mechanical ventilation (MV) is a tool used for the treatment of patients with acute or chronic respiratory failure. However, MV is a non-physiological resource, and it can cause metabolic disorders such as release of pro-inflammatory cytokines and production of reactive oxygen species. In clinical setting, maneuvers such as sigh, are used to protect the lungs. Thus, this study aimed to evaluate the effects of sigh on oxidative stress and lung inflammation in healthy adult Wistar rats submitted to MV. Male Wistar rats were divided into four groups: control (CG), mechanical ventilation (MVG), MV set at 20 sighs/h (MVG20), and MV set at 40 sighs/h (MVG40). The MVG, MVG20, and MVG40 were submitted to MV for 1 h. After the protocol, all animals were euthanized and the blood, bronchoalveolar lavage fluid, and lungs were collected for subsequent analysis. In the arterial blood, MVG40 presented higher partial pressure of oxygen and lower partial pressure of carbon dioxide compared to control. The levels of bicarbonate in MVG20 were lower compared to CG. The neutrophil influx in bronchoalveolar lavage fluid was higher in the MVG compared to CG and MVG40. In the lung parenchyma, the lipid peroxidation was higher in MVG compared to CG, MVG20, and MVG40. Superoxide dismutase and catalase activity were higher in MVG compared to CG, MVG20, and MVG40. The levels of IL-1, IL-6, and TNF in the lung homogenate were higher in MVG compared to CG, MVG20, and MVG40. The use of sigh plays a protective role as it reduced redox imbalance and pulmonary inflammation caused by MV.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>32640895</pmid><doi>10.1177/1535370220940995</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-0804-5196</orcidid><orcidid>https://orcid.org/0000-0002-1061-6445</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Aging - pathology Animals Biomarkers - metabolism Blood Gas Analysis Bronchoalveolar Lavage Fluid - cytology Hemodynamics Inflammation Mediators - metabolism Lung - pathology Lung - physiopathology Male Original Research Oxidative Stress Rats, Wistar Respiration, Artificial Respiratory Function Tests |
title | Sigh maneuver protects healthy lungs during mechanical ventilation in adult Wistar rats |
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