Piscidin 4: Genetic expression and comparative immunolocalization in Nile tilapia (Oreochromis niloticus) following challenge using different local bacterial strains
The antimicrobial activity of tilapia piscidin 4 (TP4) was determined in vitro against four bacterial strains, Aeromonas hydrophilla, Pseudomonas fluorescens, Streptococcus iniae and Vibrio anguillarum. Nile tilapia were infected with low and high doses of the tested pathogens; after 3, 6, 24 h and...
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| Veröffentlicht in: | Developmental and comparative immunology 2020-11, Vol.112, p.103777-103777, Article 103777 |
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| container_title | Developmental and comparative immunology |
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| creator | Mahrous, Karima F. Aboelenin, Mohamad M. Abd El-Kader, Heba A.M. Mabrouk, Dalia M. Gaafar, Alkhateib Y. Younes, Abdelgayed M. Mahmoud, Mahmoud A. Khalil, Wagdy K.B. Hassanane, Mohamed S. |
| description | The antimicrobial activity of tilapia piscidin 4 (TP4) was determined in vitro against four bacterial strains, Aeromonas hydrophilla, Pseudomonas fluorescens, Streptococcus iniae and Vibrio anguillarum. Nile tilapia were infected with low and high doses of the tested pathogens; after 3, 6, 24 h and 7 days of the specific TP4 gene expression, tissue immunolocalization was also performed. Histopathological examination revealed septicaemia and necrosis of hemopoietic tissue for all of the tested bacteria. Immunolocalization showed abundance in S. iniae-infected fish tissues. Quantitative RT-PCR analysis revealed that high doses raised mRNA expression levels compared to low doses and expression levels increased in the infected fish, particularly after 24 h, indicating that TP4 exerts potent bactericidal activity against some fish pathogens and plays an essential role in fish immunity.
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•Tilapia Piscidin 4 (TP4) could exert potent bactericidal activity against Streptococcus iniae.•TP4 was expressed by injection of A. hydrophilla, P. fluorescens, and V. anguillarum.•Immunostaining was detected in E.G. and goblet cells 6 h S. iniae and V. anguillarum post-infection.•Immunostaining was seen in gills and spleen 24 h P. fluorescens and A. hydrophyla post-infection.•Organs with immunostaining showed mild pathological lesions in comparison to others. |
| doi_str_mv | 10.1016/j.dci.2020.103777 |
| format | Article |
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[Display omitted]
•Tilapia Piscidin 4 (TP4) could exert potent bactericidal activity against Streptococcus iniae.•TP4 was expressed by injection of A. hydrophilla, P. fluorescens, and V. anguillarum.•Immunostaining was detected in E.G. and goblet cells 6 h S. iniae and V. anguillarum post-infection.•Immunostaining was seen in gills and spleen 24 h P. fluorescens and A. hydrophyla post-infection.•Organs with immunostaining showed mild pathological lesions in comparison to others.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/j.dci.2020.103777</identifier><identifier>PMID: 32634526</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Aeromonas hydrophilla ; Animal tissues ; Antiinfectives and antibacterials ; Antimicrobial activity ; Bacteria ; Bactericidal activity ; Fish ; Fish immunity ; Gene expression ; Necrosis ; Oreochromis niloticus ; Pathogens ; Polymerase chain reaction ; Ponds ; Pseudomonas fluorescens ; Strains (organisms) ; Streptococcus iniae ; Tilapia ; Tilapia piscidin 4 ; Tissue analysis ; Vibrio anguillarum ; Waterborne diseases</subject><ispartof>Developmental and comparative immunology, 2020-11, Vol.112, p.103777-103777, Article 103777</ispartof><rights>2020 Elsevier Ltd</rights><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. Nov 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-15895b3d252fdf5f57082a098c58e04d79aac514ec2cac842154e06569800063</citedby><cites>FETCH-LOGICAL-c381t-15895b3d252fdf5f57082a098c58e04d79aac514ec2cac842154e06569800063</cites><orcidid>0000-0003-1092-9908 ; 0000-0003-1010-4551 ; 0000-0003-3026-2581 ; 0000-0002-0598-7217 ; 0000-0002-8535-9359 ; 0000-0002-1662-1034 ; 0000-0002-9385-2549 ; 0000-0002-7744-4190 ; 0000-0003-2033-9653</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.dci.2020.103777$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32634526$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mahrous, Karima F.</creatorcontrib><creatorcontrib>Aboelenin, Mohamad M.</creatorcontrib><creatorcontrib>Abd El-Kader, Heba A.M.</creatorcontrib><creatorcontrib>Mabrouk, Dalia M.</creatorcontrib><creatorcontrib>Gaafar, Alkhateib Y.</creatorcontrib><creatorcontrib>Younes, Abdelgayed M.</creatorcontrib><creatorcontrib>Mahmoud, Mahmoud A.</creatorcontrib><creatorcontrib>Khalil, Wagdy K.B.</creatorcontrib><creatorcontrib>Hassanane, Mohamed S.</creatorcontrib><title>Piscidin 4: Genetic expression and comparative immunolocalization in Nile tilapia (Oreochromis niloticus) following challenge using different local bacterial strains</title><title>Developmental and comparative immunology</title><addtitle>Dev Comp Immunol</addtitle><description>The antimicrobial activity of tilapia piscidin 4 (TP4) was determined in vitro against four bacterial strains, Aeromonas hydrophilla, Pseudomonas fluorescens, Streptococcus iniae and Vibrio anguillarum. Nile tilapia were infected with low and high doses of the tested pathogens; after 3, 6, 24 h and 7 days of the specific TP4 gene expression, tissue immunolocalization was also performed. Histopathological examination revealed septicaemia and necrosis of hemopoietic tissue for all of the tested bacteria. Immunolocalization showed abundance in S. iniae-infected fish tissues. Quantitative RT-PCR analysis revealed that high doses raised mRNA expression levels compared to low doses and expression levels increased in the infected fish, particularly after 24 h, indicating that TP4 exerts potent bactericidal activity against some fish pathogens and plays an essential role in fish immunity.
[Display omitted]
•Tilapia Piscidin 4 (TP4) could exert potent bactericidal activity against Streptococcus iniae.•TP4 was expressed by injection of A. hydrophilla, P. fluorescens, and V. anguillarum.•Immunostaining was detected in E.G. and goblet cells 6 h S. iniae and V. anguillarum post-infection.•Immunostaining was seen in gills and spleen 24 h P. fluorescens and A. hydrophyla post-infection.•Organs with immunostaining showed mild pathological lesions in comparison to others.</description><subject>Aeromonas hydrophilla</subject><subject>Animal tissues</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial activity</subject><subject>Bacteria</subject><subject>Bactericidal activity</subject><subject>Fish</subject><subject>Fish immunity</subject><subject>Gene expression</subject><subject>Necrosis</subject><subject>Oreochromis niloticus</subject><subject>Pathogens</subject><subject>Polymerase chain reaction</subject><subject>Ponds</subject><subject>Pseudomonas fluorescens</subject><subject>Strains (organisms)</subject><subject>Streptococcus iniae</subject><subject>Tilapia</subject><subject>Tilapia piscidin 4</subject><subject>Tissue analysis</subject><subject>Vibrio anguillarum</subject><subject>Waterborne diseases</subject><issn>0145-305X</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kc1u1TAQhS0EopfCA7BBltiURS62EycOrFAFBamiLLpgZ_nak3auHDvYSfl5n75nHW5hwQJv7LG-czQzh5DnnG054-3r_dZZ3Aom1rruuu4B2XDV9RVjqn9INow3sqqZ_HpEnuS8Z-Uozh6To1q0dSNFuyG3XzBbdBho84aeQYAZLYUfU4KcMQZqgqM2jpNJZsYboDiOS4g-WuPxV_kqSNF-Rg90Rm8mNPTkIkG01ymOmGlAH4vlkl_RIXofv2O4ovbaeA_hCuiS19rhMECCMNPfxnRn7AwJyyvPyWDIT8mjwfgMz-7vY3L54f3l6cfq_OLs0-m788rWis8Vl6qXu9oJKQY3yEF2TAnDemWlAta4rjfGSt6AFdZY1QguG2CtbHtVVtPWx-TkYDul-G2BPOsyggXvTYC4ZC2KopFdy3hBX_6D7uOSQmmuUE0nu5q1qyE_UDbFnBMMeko4mvRTc6bXCPVelwj1GqE-RFg0L-6dl90I7q_iT2YFeHsAoGziBiHpkiAECw4T2Fm7iP-xvwNGra5O</recordid><startdate>202011</startdate><enddate>202011</enddate><creator>Mahrous, Karima F.</creator><creator>Aboelenin, Mohamad M.</creator><creator>Abd El-Kader, Heba A.M.</creator><creator>Mabrouk, Dalia M.</creator><creator>Gaafar, Alkhateib Y.</creator><creator>Younes, Abdelgayed M.</creator><creator>Mahmoud, Mahmoud A.</creator><creator>Khalil, Wagdy K.B.</creator><creator>Hassanane, Mohamed S.</creator><general>Elsevier Ltd</general><general>Elsevier Science Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1092-9908</orcidid><orcidid>https://orcid.org/0000-0003-1010-4551</orcidid><orcidid>https://orcid.org/0000-0003-3026-2581</orcidid><orcidid>https://orcid.org/0000-0002-0598-7217</orcidid><orcidid>https://orcid.org/0000-0002-8535-9359</orcidid><orcidid>https://orcid.org/0000-0002-1662-1034</orcidid><orcidid>https://orcid.org/0000-0002-9385-2549</orcidid><orcidid>https://orcid.org/0000-0002-7744-4190</orcidid><orcidid>https://orcid.org/0000-0003-2033-9653</orcidid></search><sort><creationdate>202011</creationdate><title>Piscidin 4: Genetic expression and comparative immunolocalization in Nile tilapia (Oreochromis niloticus) following challenge using different local bacterial strains</title><author>Mahrous, Karima F. ; Aboelenin, Mohamad M. ; Abd El-Kader, Heba A.M. ; Mabrouk, Dalia M. ; Gaafar, Alkhateib Y. ; Younes, Abdelgayed M. ; Mahmoud, Mahmoud A. ; Khalil, Wagdy K.B. ; Hassanane, Mohamed S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-15895b3d252fdf5f57082a098c58e04d79aac514ec2cac842154e06569800063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aeromonas hydrophilla</topic><topic>Animal tissues</topic><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial activity</topic><topic>Bacteria</topic><topic>Bactericidal activity</topic><topic>Fish</topic><topic>Fish immunity</topic><topic>Gene expression</topic><topic>Necrosis</topic><topic>Oreochromis niloticus</topic><topic>Pathogens</topic><topic>Polymerase chain reaction</topic><topic>Ponds</topic><topic>Pseudomonas fluorescens</topic><topic>Strains (organisms)</topic><topic>Streptococcus iniae</topic><topic>Tilapia</topic><topic>Tilapia piscidin 4</topic><topic>Tissue analysis</topic><topic>Vibrio anguillarum</topic><topic>Waterborne diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mahrous, Karima F.</creatorcontrib><creatorcontrib>Aboelenin, Mohamad M.</creatorcontrib><creatorcontrib>Abd El-Kader, Heba A.M.</creatorcontrib><creatorcontrib>Mabrouk, Dalia M.</creatorcontrib><creatorcontrib>Gaafar, Alkhateib Y.</creatorcontrib><creatorcontrib>Younes, Abdelgayed M.</creatorcontrib><creatorcontrib>Mahmoud, Mahmoud A.</creatorcontrib><creatorcontrib>Khalil, Wagdy K.B.</creatorcontrib><creatorcontrib>Hassanane, Mohamed S.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mahrous, Karima F.</au><au>Aboelenin, Mohamad M.</au><au>Abd El-Kader, Heba A.M.</au><au>Mabrouk, Dalia M.</au><au>Gaafar, Alkhateib Y.</au><au>Younes, Abdelgayed M.</au><au>Mahmoud, Mahmoud A.</au><au>Khalil, Wagdy K.B.</au><au>Hassanane, Mohamed S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Piscidin 4: Genetic expression and comparative immunolocalization in Nile tilapia (Oreochromis niloticus) following challenge using different local bacterial strains</atitle><jtitle>Developmental and comparative immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>2020-11</date><risdate>2020</risdate><volume>112</volume><spage>103777</spage><epage>103777</epage><pages>103777-103777</pages><artnum>103777</artnum><issn>0145-305X</issn><eissn>1879-0089</eissn><abstract>The antimicrobial activity of tilapia piscidin 4 (TP4) was determined in vitro against four bacterial strains, Aeromonas hydrophilla, Pseudomonas fluorescens, Streptococcus iniae and Vibrio anguillarum. Nile tilapia were infected with low and high doses of the tested pathogens; after 3, 6, 24 h and 7 days of the specific TP4 gene expression, tissue immunolocalization was also performed. Histopathological examination revealed septicaemia and necrosis of hemopoietic tissue for all of the tested bacteria. Immunolocalization showed abundance in S. iniae-infected fish tissues. Quantitative RT-PCR analysis revealed that high doses raised mRNA expression levels compared to low doses and expression levels increased in the infected fish, particularly after 24 h, indicating that TP4 exerts potent bactericidal activity against some fish pathogens and plays an essential role in fish immunity.
[Display omitted]
•Tilapia Piscidin 4 (TP4) could exert potent bactericidal activity against Streptococcus iniae.•TP4 was expressed by injection of A. hydrophilla, P. fluorescens, and V. anguillarum.•Immunostaining was detected in E.G. and goblet cells 6 h S. iniae and V. anguillarum post-infection.•Immunostaining was seen in gills and spleen 24 h P. fluorescens and A. hydrophyla post-infection.•Organs with immunostaining showed mild pathological lesions in comparison to others.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>32634526</pmid><doi>10.1016/j.dci.2020.103777</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-1092-9908</orcidid><orcidid>https://orcid.org/0000-0003-1010-4551</orcidid><orcidid>https://orcid.org/0000-0003-3026-2581</orcidid><orcidid>https://orcid.org/0000-0002-0598-7217</orcidid><orcidid>https://orcid.org/0000-0002-8535-9359</orcidid><orcidid>https://orcid.org/0000-0002-1662-1034</orcidid><orcidid>https://orcid.org/0000-0002-9385-2549</orcidid><orcidid>https://orcid.org/0000-0002-7744-4190</orcidid><orcidid>https://orcid.org/0000-0003-2033-9653</orcidid></addata></record> |
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| ispartof | Developmental and comparative immunology, 2020-11, Vol.112, p.103777-103777, Article 103777 |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Aeromonas hydrophilla Animal tissues Antiinfectives and antibacterials Antimicrobial activity Bacteria Bactericidal activity Fish Fish immunity Gene expression Necrosis Oreochromis niloticus Pathogens Polymerase chain reaction Ponds Pseudomonas fluorescens Strains (organisms) Streptococcus iniae Tilapia Tilapia piscidin 4 Tissue analysis Vibrio anguillarum Waterborne diseases |
| title | Piscidin 4: Genetic expression and comparative immunolocalization in Nile tilapia (Oreochromis niloticus) following challenge using different local bacterial strains |
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