Enhanced oxidative stress and damage in glycated erythrocytes
Diabetes is associated with a dramatic mortality rate due to its vascular complications. Chronic hyperglycemia in diabetes leads to enhanced glycation of erythrocytes and oxidative stress. Even though erythrocytes play a determining role in vascular complications, very little is known about how eryt...
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creator | Turpin, Chloe Catan, Aurelie Guerin-Dubourg, Alexis Debussche, Xavier Bravo, Susana B. Alvarez, Ezequiel Van den Elsen, Jean Meilhac, Olivier Rondeau, Philippe Bourdon, Emmanuel |
description | Diabetes is associated with a dramatic mortality rate due to its vascular complications. Chronic hyperglycemia in diabetes leads to enhanced glycation of erythrocytes and oxidative stress. Even though erythrocytes play a determining role in vascular complications, very little is known about how erythrocyte structure and functionality can be affected by glycation. Our objective was to decipher the impact of glycation on erythrocyte structure, oxidative stress parameters and capacity to interact with cultured human endothelial cells.In vitroglycated erythrocytes were prepared following incubation in the presence of different concentrations of glucose. To get insight into thein vivorelevance of our results, we compared these data to those obtained using red blood cells purified from diabetics or non-diabetics. We measured erythrocyte deformability, susceptibility to hemolysis, reactive oxygen species production and oxidative damage accumulation. Altered structures, redox status and oxidative modifications were increased in glycated erythrocytes. These modifications were associated with reduced antioxidant defence mediated by enzymatic activity. Enhanced erythrocyte phagocytosis by endothelial cells was observed when cultured with glycated erythrocytes, which was associated with increased levels of phosphatidylserine-likely as a result of an eryptosis phenomenon triggered by the hyperglycemic treatment. Most types of oxidative damage identified inin vitroglycated erythrocytes were also observed in red blood cells isolated from diabetics. These results bring new insights into the impact of glycation on erythrocyte structure, oxidative damage and their capacity to interact with endothelial cells, with a possible relevance to diabetes. |
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Chronic hyperglycemia in diabetes leads to enhanced glycation of erythrocytes and oxidative stress. Even though erythrocytes play a determining role in vascular complications, very little is known about how erythrocyte structure and functionality can be affected by glycation. Our objective was to decipher the impact of glycation on erythrocyte structure, oxidative stress parameters and capacity to interact with cultured human endothelial cells.In vitroglycated erythrocytes were prepared following incubation in the presence of different concentrations of glucose. To get insight into thein vivorelevance of our results, we compared these data to those obtained using red blood cells purified from diabetics or non-diabetics. We measured erythrocyte deformability, susceptibility to hemolysis, reactive oxygen species production and oxidative damage accumulation. Altered structures, redox status and oxidative modifications were increased in glycated erythrocytes. These modifications were associated with reduced antioxidant defence mediated by enzymatic activity. Enhanced erythrocyte phagocytosis by endothelial cells was observed when cultured with glycated erythrocytes, which was associated with increased levels of phosphatidylserine-likely as a result of an eryptosis phenomenon triggered by the hyperglycemic treatment. Most types of oxidative damage identified inin vitroglycated erythrocytes were also observed in red blood cells isolated from diabetics. These results bring new insights into the impact of glycation on erythrocyte structure, oxidative damage and their capacity to interact with endothelial cells, with a possible relevance to diabetes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0235335</identifier><identifier>PMID: 32628695</identifier><language>eng</language><publisher>SAN FRANCISCO: Public Library Science</publisher><subject>Antioxidants ; Biology and Life Sciences ; Blood ; Blood cells ; Cardiology and cardiovascular system ; Cardiovascular disease ; Cellular Biology ; Complications ; Damage accumulation ; Damage detection ; Deformability ; Diabetes ; Diabetes mellitus ; Diabetes research ; Endothelial cells ; Enzymatic activity ; Erythrocytes ; Formability ; Glucose ; Glycosylation ; Health aspects ; Hematology ; Hemoglobin ; Human health and pathology ; Hyperglycemia ; Impact damage ; Incubation ; Life Sciences ; Medicine and Health Sciences ; Mortality ; Multidisciplinary Sciences ; Oxidative stress ; Oxygen ; Phagocytosis ; Phosphatidylserine ; Physical Sciences ; Proteins ; Reactive oxygen species ; Red blood cells ; Science & Technology ; Science & Technology - Other Topics ; Structure</subject><ispartof>PloS one, 2020-07, Vol.15 (7), p.e0235335-e0235335, Article 0235335</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Turpin et al. 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Chronic hyperglycemia in diabetes leads to enhanced glycation of erythrocytes and oxidative stress. Even though erythrocytes play a determining role in vascular complications, very little is known about how erythrocyte structure and functionality can be affected by glycation. Our objective was to decipher the impact of glycation on erythrocyte structure, oxidative stress parameters and capacity to interact with cultured human endothelial cells.In vitroglycated erythrocytes were prepared following incubation in the presence of different concentrations of glucose. To get insight into thein vivorelevance of our results, we compared these data to those obtained using red blood cells purified from diabetics or non-diabetics. We measured erythrocyte deformability, susceptibility to hemolysis, reactive oxygen species production and oxidative damage accumulation. Altered structures, redox status and oxidative modifications were increased in glycated erythrocytes. These modifications were associated with reduced antioxidant defence mediated by enzymatic activity. Enhanced erythrocyte phagocytosis by endothelial cells was observed when cultured with glycated erythrocytes, which was associated with increased levels of phosphatidylserine-likely as a result of an eryptosis phenomenon triggered by the hyperglycemic treatment. Most types of oxidative damage identified inin vitroglycated erythrocytes were also observed in red blood cells isolated from diabetics. These results bring new insights into the impact of glycation on erythrocyte structure, oxidative damage and their capacity to interact with endothelial cells, with a possible relevance to diabetes.</description><subject>Antioxidants</subject><subject>Biology and Life Sciences</subject><subject>Blood</subject><subject>Blood cells</subject><subject>Cardiology and cardiovascular system</subject><subject>Cardiovascular disease</subject><subject>Cellular Biology</subject><subject>Complications</subject><subject>Damage accumulation</subject><subject>Damage detection</subject><subject>Deformability</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes research</subject><subject>Endothelial cells</subject><subject>Enzymatic activity</subject><subject>Erythrocytes</subject><subject>Formability</subject><subject>Glucose</subject><subject>Glycosylation</subject><subject>Health aspects</subject><subject>Hematology</subject><subject>Hemoglobin</subject><subject>Human health and pathology</subject><subject>Hyperglycemia</subject><subject>Impact damage</subject><subject>Incubation</subject><subject>Life Sciences</subject><subject>Medicine and Health Sciences</subject><subject>Mortality</subject><subject>Multidisciplinary Sciences</subject><subject>Oxidative stress</subject><subject>Oxygen</subject><subject>Phagocytosis</subject><subject>Phosphatidylserine</subject><subject>Physical Sciences</subject><subject>Proteins</subject><subject>Reactive oxygen species</subject><subject>Red blood cells</subject><subject>Science & Technology</subject><subject>Science & Technology - Other 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oxidative stress and damage in glycated erythrocytes</title><author>Turpin, Chloe ; Catan, Aurelie ; Guerin-Dubourg, Alexis ; Debussche, Xavier ; Bravo, Susana B. ; Alvarez, Ezequiel ; Van den Elsen, Jean ; Meilhac, Olivier ; Rondeau, Philippe ; Bourdon, Emmanuel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c706t-300d9be0b747145e2d04fab2cf289a3a1eea2a4270ce866a0a2cd72e242c95cb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antioxidants</topic><topic>Biology and Life Sciences</topic><topic>Blood</topic><topic>Blood cells</topic><topic>Cardiology and cardiovascular system</topic><topic>Cardiovascular disease</topic><topic>Cellular Biology</topic><topic>Complications</topic><topic>Damage accumulation</topic><topic>Damage detection</topic><topic>Deformability</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Diabetes research</topic><topic>Endothelial 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ONE</stitle><date>2020-07-06</date><risdate>2020</risdate><volume>15</volume><issue>7</issue><spage>e0235335</spage><epage>e0235335</epage><pages>e0235335-e0235335</pages><artnum>0235335</artnum><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Diabetes is associated with a dramatic mortality rate due to its vascular complications. Chronic hyperglycemia in diabetes leads to enhanced glycation of erythrocytes and oxidative stress. Even though erythrocytes play a determining role in vascular complications, very little is known about how erythrocyte structure and functionality can be affected by glycation. Our objective was to decipher the impact of glycation on erythrocyte structure, oxidative stress parameters and capacity to interact with cultured human endothelial cells.In vitroglycated erythrocytes were prepared following incubation in the presence of different concentrations of glucose. To get insight into thein vivorelevance of our results, we compared these data to those obtained using red blood cells purified from diabetics or non-diabetics. We measured erythrocyte deformability, susceptibility to hemolysis, reactive oxygen species production and oxidative damage accumulation. Altered structures, redox status and oxidative modifications were increased in glycated erythrocytes. These modifications were associated with reduced antioxidant defence mediated by enzymatic activity. Enhanced erythrocyte phagocytosis by endothelial cells was observed when cultured with glycated erythrocytes, which was associated with increased levels of phosphatidylserine-likely as a result of an eryptosis phenomenon triggered by the hyperglycemic treatment. Most types of oxidative damage identified inin vitroglycated erythrocytes were also observed in red blood cells isolated from diabetics. These results bring new insights into the impact of glycation on erythrocyte structure, oxidative damage and their capacity to interact with endothelial cells, with a possible relevance to diabetes.</abstract><cop>SAN FRANCISCO</cop><pub>Public Library Science</pub><pmid>32628695</pmid><doi>10.1371/journal.pone.0235335</doi><tpages>19</tpages><orcidid>https://orcid.org/0000-0002-2381-8425</orcidid><orcidid>https://orcid.org/0000-0003-3087-4297</orcidid><orcidid>https://orcid.org/0000-0003-3731-150X</orcidid><orcidid>https://orcid.org/0000-0002-7864-1081</orcidid><orcidid>https://orcid.org/0000-0002-3740-7539</orcidid><orcidid>https://orcid.org/0000-0002-4652-1376</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Antioxidants Biology and Life Sciences Blood Blood cells Cardiology and cardiovascular system Cardiovascular disease Cellular Biology Complications Damage accumulation Damage detection Deformability Diabetes Diabetes mellitus Diabetes research Endothelial cells Enzymatic activity Erythrocytes Formability Glucose Glycosylation Health aspects Hematology Hemoglobin Human health and pathology Hyperglycemia Impact damage Incubation Life Sciences Medicine and Health Sciences Mortality Multidisciplinary Sciences Oxidative stress Oxygen Phagocytosis Phosphatidylserine Physical Sciences Proteins Reactive oxygen species Red blood cells Science & Technology Science & Technology - Other Topics Structure |
title | Enhanced oxidative stress and damage in glycated erythrocytes |
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