Enhanced oxidative stress and damage in glycated erythrocytes
Diabetes is associated with a dramatic mortality rate due to its vascular complications. Chronic hyperglycemia in diabetes leads to enhanced glycation of erythrocytes and oxidative stress. Even though erythrocytes play a determining role in vascular complications, very little is known about how eryt...
Gespeichert in:
Veröffentlicht in: | PloS one 2020-07, Vol.15 (7), p.e0235335-e0235335, Article 0235335 |
---|---|
Hauptverfasser: | , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | e0235335 |
---|---|
container_issue | 7 |
container_start_page | e0235335 |
container_title | PloS one |
container_volume | 15 |
creator | Turpin, Chloe Catan, Aurelie Guerin-Dubourg, Alexis Debussche, Xavier Bravo, Susana B. Alvarez, Ezequiel Van den Elsen, Jean Meilhac, Olivier Rondeau, Philippe Bourdon, Emmanuel |
description | Diabetes is associated with a dramatic mortality rate due to its vascular complications. Chronic hyperglycemia in diabetes leads to enhanced glycation of erythrocytes and oxidative stress. Even though erythrocytes play a determining role in vascular complications, very little is known about how erythrocyte structure and functionality can be affected by glycation. Our objective was to decipher the impact of glycation on erythrocyte structure, oxidative stress parameters and capacity to interact with cultured human endothelial cells.In vitroglycated erythrocytes were prepared following incubation in the presence of different concentrations of glucose. To get insight into thein vivorelevance of our results, we compared these data to those obtained using red blood cells purified from diabetics or non-diabetics. We measured erythrocyte deformability, susceptibility to hemolysis, reactive oxygen species production and oxidative damage accumulation. Altered structures, redox status and oxidative modifications were increased in glycated erythrocytes. These modifications were associated with reduced antioxidant defence mediated by enzymatic activity. Enhanced erythrocyte phagocytosis by endothelial cells was observed when cultured with glycated erythrocytes, which was associated with increased levels of phosphatidylserine-likely as a result of an eryptosis phenomenon triggered by the hyperglycemic treatment. Most types of oxidative damage identified inin vitroglycated erythrocytes were also observed in red blood cells isolated from diabetics. These results bring new insights into the impact of glycation on erythrocyte structure, oxidative damage and their capacity to interact with endothelial cells, with a possible relevance to diabetes. |
doi_str_mv | 10.1371/journal.pone.0235335 |
format | Article |
fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2421122768</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A628712528</galeid><doaj_id>oai_doaj_org_article_929c6c7e370b414ab4a6d762d4f7612c</doaj_id><sourcerecordid>A628712528</sourcerecordid><originalsourceid>FETCH-LOGICAL-c706t-300d9be0b747145e2d04fab2cf289a3a1eea2a4270ce866a0a2cd72e242c95cb3</originalsourceid><addsrcrecordid>eNqNk11r2zAUhs3YWLts_2CwwGBsbMn0YVv2xQYhdGshUNjXrTiWj20FR0olOWv-_ZQmK03pRbGNhPS8r8451kmS15RMKRf089IOzkA_XVuDU8J4xnn2JDmlJWeTnBH-9M78JHnh_ZKQjBd5_jw54SxnRV5mp8mXM9OBUViP7bWuIegNjn1w6P0YTD2uYQUtjrUZt_1WQYgcum3onFXbgP5l8qyB3uOrwzhKfn87-zU_nywuv1_MZ4uJEiQPE05IXVZIKpEKmmbIapI2UDHVsKIEDhQRGKRMEIUxQCDAVC0YspSpMlMVHyVv9r7r3np5yNzLuE-ykmfxGyUXe6K2sJRrp1fgttKCljcL1rUSXNCqR1myUuVKIBekSmkKVQp5LXJWp43IKVPR6-vhtKFaYa3QBAf9kenxjtGdbO1GCs7jy6PBp71Bd092PltIbTy6lSSspBkp-IZG_P3hPGevBvRBrrRX2Pdg0A43aVLKmMiLiL69hz5cjAPVQsxXm8bGMNXOVM7ijxeUZWznNX2Aik-NK63irWp0XD8SfDgSRCbgdWhh8F5e_PzxePbyzzH77g7bIfSh87YfgrbGH4PpHlTOeu-wuS0uJXLXFP-rIXdNIQ9NEWUf97K_WNnGK43xwt9KSWyLjJC8EHHGWKSLx9NzHWAX5twOJvB__5IcLw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2420593559</pqid></control><display><type>article</type><title>Enhanced oxidative stress and damage in glycated erythrocytes</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>Web of Science - Science Citation Index Expanded - 2020<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /></source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Turpin, Chloe ; Catan, Aurelie ; Guerin-Dubourg, Alexis ; Debussche, Xavier ; Bravo, Susana B. ; Alvarez, Ezequiel ; Van den Elsen, Jean ; Meilhac, Olivier ; Rondeau, Philippe ; Bourdon, Emmanuel</creator><contributor>Song, Ping</contributor><creatorcontrib>Turpin, Chloe ; Catan, Aurelie ; Guerin-Dubourg, Alexis ; Debussche, Xavier ; Bravo, Susana B. ; Alvarez, Ezequiel ; Van den Elsen, Jean ; Meilhac, Olivier ; Rondeau, Philippe ; Bourdon, Emmanuel ; Song, Ping</creatorcontrib><description>Diabetes is associated with a dramatic mortality rate due to its vascular complications. Chronic hyperglycemia in diabetes leads to enhanced glycation of erythrocytes and oxidative stress. Even though erythrocytes play a determining role in vascular complications, very little is known about how erythrocyte structure and functionality can be affected by glycation. Our objective was to decipher the impact of glycation on erythrocyte structure, oxidative stress parameters and capacity to interact with cultured human endothelial cells.In vitroglycated erythrocytes were prepared following incubation in the presence of different concentrations of glucose. To get insight into thein vivorelevance of our results, we compared these data to those obtained using red blood cells purified from diabetics or non-diabetics. We measured erythrocyte deformability, susceptibility to hemolysis, reactive oxygen species production and oxidative damage accumulation. Altered structures, redox status and oxidative modifications were increased in glycated erythrocytes. These modifications were associated with reduced antioxidant defence mediated by enzymatic activity. Enhanced erythrocyte phagocytosis by endothelial cells was observed when cultured with glycated erythrocytes, which was associated with increased levels of phosphatidylserine-likely as a result of an eryptosis phenomenon triggered by the hyperglycemic treatment. Most types of oxidative damage identified inin vitroglycated erythrocytes were also observed in red blood cells isolated from diabetics. These results bring new insights into the impact of glycation on erythrocyte structure, oxidative damage and their capacity to interact with endothelial cells, with a possible relevance to diabetes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0235335</identifier><identifier>PMID: 32628695</identifier><language>eng</language><publisher>SAN FRANCISCO: Public Library Science</publisher><subject>Antioxidants ; Biology and Life Sciences ; Blood ; Blood cells ; Cardiology and cardiovascular system ; Cardiovascular disease ; Cellular Biology ; Complications ; Damage accumulation ; Damage detection ; Deformability ; Diabetes ; Diabetes mellitus ; Diabetes research ; Endothelial cells ; Enzymatic activity ; Erythrocytes ; Formability ; Glucose ; Glycosylation ; Health aspects ; Hematology ; Hemoglobin ; Human health and pathology ; Hyperglycemia ; Impact damage ; Incubation ; Life Sciences ; Medicine and Health Sciences ; Mortality ; Multidisciplinary Sciences ; Oxidative stress ; Oxygen ; Phagocytosis ; Phosphatidylserine ; Physical Sciences ; Proteins ; Reactive oxygen species ; Red blood cells ; Science & Technology ; Science & Technology - Other Topics ; Structure</subject><ispartof>PloS one, 2020-07, Vol.15 (7), p.e0235335-e0235335, Article 0235335</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Turpin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>2020 Turpin et al 2020 Turpin et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>40</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000550068700022</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c706t-300d9be0b747145e2d04fab2cf289a3a1eea2a4270ce866a0a2cd72e242c95cb3</citedby><cites>FETCH-LOGICAL-c706t-300d9be0b747145e2d04fab2cf289a3a1eea2a4270ce866a0a2cd72e242c95cb3</cites><orcidid>0000-0002-2381-8425 ; 0000-0003-3087-4297 ; 0000-0003-3731-150X ; 0000-0002-7864-1081 ; 0000-0002-3740-7539 ; 0000-0002-4652-1376</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7337333/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7337333/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2104,2116,2930,23873,27931,27932,28255,53798,53800</link.rule.ids><backlink>$$Uhttps://inserm.hal.science/inserm-02915083$$DView record in HAL$$Hfree_for_read</backlink></links><search><contributor>Song, Ping</contributor><creatorcontrib>Turpin, Chloe</creatorcontrib><creatorcontrib>Catan, Aurelie</creatorcontrib><creatorcontrib>Guerin-Dubourg, Alexis</creatorcontrib><creatorcontrib>Debussche, Xavier</creatorcontrib><creatorcontrib>Bravo, Susana B.</creatorcontrib><creatorcontrib>Alvarez, Ezequiel</creatorcontrib><creatorcontrib>Van den Elsen, Jean</creatorcontrib><creatorcontrib>Meilhac, Olivier</creatorcontrib><creatorcontrib>Rondeau, Philippe</creatorcontrib><creatorcontrib>Bourdon, Emmanuel</creatorcontrib><title>Enhanced oxidative stress and damage in glycated erythrocytes</title><title>PloS one</title><addtitle>PLOS ONE</addtitle><description>Diabetes is associated with a dramatic mortality rate due to its vascular complications. Chronic hyperglycemia in diabetes leads to enhanced glycation of erythrocytes and oxidative stress. Even though erythrocytes play a determining role in vascular complications, very little is known about how erythrocyte structure and functionality can be affected by glycation. Our objective was to decipher the impact of glycation on erythrocyte structure, oxidative stress parameters and capacity to interact with cultured human endothelial cells.In vitroglycated erythrocytes were prepared following incubation in the presence of different concentrations of glucose. To get insight into thein vivorelevance of our results, we compared these data to those obtained using red blood cells purified from diabetics or non-diabetics. We measured erythrocyte deformability, susceptibility to hemolysis, reactive oxygen species production and oxidative damage accumulation. Altered structures, redox status and oxidative modifications were increased in glycated erythrocytes. These modifications were associated with reduced antioxidant defence mediated by enzymatic activity. Enhanced erythrocyte phagocytosis by endothelial cells was observed when cultured with glycated erythrocytes, which was associated with increased levels of phosphatidylserine-likely as a result of an eryptosis phenomenon triggered by the hyperglycemic treatment. Most types of oxidative damage identified inin vitroglycated erythrocytes were also observed in red blood cells isolated from diabetics. These results bring new insights into the impact of glycation on erythrocyte structure, oxidative damage and their capacity to interact with endothelial cells, with a possible relevance to diabetes.</description><subject>Antioxidants</subject><subject>Biology and Life Sciences</subject><subject>Blood</subject><subject>Blood cells</subject><subject>Cardiology and cardiovascular system</subject><subject>Cardiovascular disease</subject><subject>Cellular Biology</subject><subject>Complications</subject><subject>Damage accumulation</subject><subject>Damage detection</subject><subject>Deformability</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes research</subject><subject>Endothelial cells</subject><subject>Enzymatic activity</subject><subject>Erythrocytes</subject><subject>Formability</subject><subject>Glucose</subject><subject>Glycosylation</subject><subject>Health aspects</subject><subject>Hematology</subject><subject>Hemoglobin</subject><subject>Human health and pathology</subject><subject>Hyperglycemia</subject><subject>Impact damage</subject><subject>Incubation</subject><subject>Life Sciences</subject><subject>Medicine and Health Sciences</subject><subject>Mortality</subject><subject>Multidisciplinary Sciences</subject><subject>Oxidative stress</subject><subject>Oxygen</subject><subject>Phagocytosis</subject><subject>Phosphatidylserine</subject><subject>Physical Sciences</subject><subject>Proteins</subject><subject>Reactive oxygen species</subject><subject>Red blood cells</subject><subject>Science & Technology</subject><subject>Science & Technology - Other Topics</subject><subject>Structure</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AOWDO</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11r2zAUhs3YWLts_2CwwGBsbMn0YVv2xQYhdGshUNjXrTiWj20FR0olOWv-_ZQmK03pRbGNhPS8r8451kmS15RMKRf089IOzkA_XVuDU8J4xnn2JDmlJWeTnBH-9M78JHnh_ZKQjBd5_jw54SxnRV5mp8mXM9OBUViP7bWuIegNjn1w6P0YTD2uYQUtjrUZt_1WQYgcum3onFXbgP5l8qyB3uOrwzhKfn87-zU_nywuv1_MZ4uJEiQPE05IXVZIKpEKmmbIapI2UDHVsKIEDhQRGKRMEIUxQCDAVC0YspSpMlMVHyVv9r7r3np5yNzLuE-ykmfxGyUXe6K2sJRrp1fgttKCljcL1rUSXNCqR1myUuVKIBekSmkKVQp5LXJWp43IKVPR6-vhtKFaYa3QBAf9kenxjtGdbO1GCs7jy6PBp71Bd092PltIbTy6lSSspBkp-IZG_P3hPGevBvRBrrRX2Pdg0A43aVLKmMiLiL69hz5cjAPVQsxXm8bGMNXOVM7ijxeUZWznNX2Aik-NK63irWp0XD8SfDgSRCbgdWhh8F5e_PzxePbyzzH77g7bIfSh87YfgrbGH4PpHlTOeu-wuS0uJXLXFP-rIXdNIQ9NEWUf97K_WNnGK43xwt9KSWyLjJC8EHHGWKSLx9NzHWAX5twOJvB__5IcLw</recordid><startdate>20200706</startdate><enddate>20200706</enddate><creator>Turpin, Chloe</creator><creator>Catan, Aurelie</creator><creator>Guerin-Dubourg, Alexis</creator><creator>Debussche, Xavier</creator><creator>Bravo, Susana B.</creator><creator>Alvarez, Ezequiel</creator><creator>Van den Elsen, Jean</creator><creator>Meilhac, Olivier</creator><creator>Rondeau, Philippe</creator><creator>Bourdon, Emmanuel</creator><general>Public Library Science</general><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>AOWDO</scope><scope>BLEPL</scope><scope>DTL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-2381-8425</orcidid><orcidid>https://orcid.org/0000-0003-3087-4297</orcidid><orcidid>https://orcid.org/0000-0003-3731-150X</orcidid><orcidid>https://orcid.org/0000-0002-7864-1081</orcidid><orcidid>https://orcid.org/0000-0002-3740-7539</orcidid><orcidid>https://orcid.org/0000-0002-4652-1376</orcidid></search><sort><creationdate>20200706</creationdate><title>Enhanced oxidative stress and damage in glycated erythrocytes</title><author>Turpin, Chloe ; Catan, Aurelie ; Guerin-Dubourg, Alexis ; Debussche, Xavier ; Bravo, Susana B. ; Alvarez, Ezequiel ; Van den Elsen, Jean ; Meilhac, Olivier ; Rondeau, Philippe ; Bourdon, Emmanuel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c706t-300d9be0b747145e2d04fab2cf289a3a1eea2a4270ce866a0a2cd72e242c95cb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antioxidants</topic><topic>Biology and Life Sciences</topic><topic>Blood</topic><topic>Blood cells</topic><topic>Cardiology and cardiovascular system</topic><topic>Cardiovascular disease</topic><topic>Cellular Biology</topic><topic>Complications</topic><topic>Damage accumulation</topic><topic>Damage detection</topic><topic>Deformability</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Diabetes research</topic><topic>Endothelial cells</topic><topic>Enzymatic activity</topic><topic>Erythrocytes</topic><topic>Formability</topic><topic>Glucose</topic><topic>Glycosylation</topic><topic>Health aspects</topic><topic>Hematology</topic><topic>Hemoglobin</topic><topic>Human health and pathology</topic><topic>Hyperglycemia</topic><topic>Impact damage</topic><topic>Incubation</topic><topic>Life Sciences</topic><topic>Medicine and Health Sciences</topic><topic>Mortality</topic><topic>Multidisciplinary Sciences</topic><topic>Oxidative stress</topic><topic>Oxygen</topic><topic>Phagocytosis</topic><topic>Phosphatidylserine</topic><topic>Physical Sciences</topic><topic>Proteins</topic><topic>Reactive oxygen species</topic><topic>Red blood cells</topic><topic>Science & Technology</topic><topic>Science & Technology - Other Topics</topic><topic>Structure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Turpin, Chloe</creatorcontrib><creatorcontrib>Catan, Aurelie</creatorcontrib><creatorcontrib>Guerin-Dubourg, Alexis</creatorcontrib><creatorcontrib>Debussche, Xavier</creatorcontrib><creatorcontrib>Bravo, Susana B.</creatorcontrib><creatorcontrib>Alvarez, Ezequiel</creatorcontrib><creatorcontrib>Van den Elsen, Jean</creatorcontrib><creatorcontrib>Meilhac, Olivier</creatorcontrib><creatorcontrib>Rondeau, Philippe</creatorcontrib><creatorcontrib>Bourdon, Emmanuel</creatorcontrib><collection>Web of Science - Science Citation Index Expanded - 2020</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Turpin, Chloe</au><au>Catan, Aurelie</au><au>Guerin-Dubourg, Alexis</au><au>Debussche, Xavier</au><au>Bravo, Susana B.</au><au>Alvarez, Ezequiel</au><au>Van den Elsen, Jean</au><au>Meilhac, Olivier</au><au>Rondeau, Philippe</au><au>Bourdon, Emmanuel</au><au>Song, Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced oxidative stress and damage in glycated erythrocytes</atitle><jtitle>PloS one</jtitle><stitle>PLOS ONE</stitle><date>2020-07-06</date><risdate>2020</risdate><volume>15</volume><issue>7</issue><spage>e0235335</spage><epage>e0235335</epage><pages>e0235335-e0235335</pages><artnum>0235335</artnum><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Diabetes is associated with a dramatic mortality rate due to its vascular complications. Chronic hyperglycemia in diabetes leads to enhanced glycation of erythrocytes and oxidative stress. Even though erythrocytes play a determining role in vascular complications, very little is known about how erythrocyte structure and functionality can be affected by glycation. Our objective was to decipher the impact of glycation on erythrocyte structure, oxidative stress parameters and capacity to interact with cultured human endothelial cells.In vitroglycated erythrocytes were prepared following incubation in the presence of different concentrations of glucose. To get insight into thein vivorelevance of our results, we compared these data to those obtained using red blood cells purified from diabetics or non-diabetics. We measured erythrocyte deformability, susceptibility to hemolysis, reactive oxygen species production and oxidative damage accumulation. Altered structures, redox status and oxidative modifications were increased in glycated erythrocytes. These modifications were associated with reduced antioxidant defence mediated by enzymatic activity. Enhanced erythrocyte phagocytosis by endothelial cells was observed when cultured with glycated erythrocytes, which was associated with increased levels of phosphatidylserine-likely as a result of an eryptosis phenomenon triggered by the hyperglycemic treatment. Most types of oxidative damage identified inin vitroglycated erythrocytes were also observed in red blood cells isolated from diabetics. These results bring new insights into the impact of glycation on erythrocyte structure, oxidative damage and their capacity to interact with endothelial cells, with a possible relevance to diabetes.</abstract><cop>SAN FRANCISCO</cop><pub>Public Library Science</pub><pmid>32628695</pmid><doi>10.1371/journal.pone.0235335</doi><tpages>19</tpages><orcidid>https://orcid.org/0000-0002-2381-8425</orcidid><orcidid>https://orcid.org/0000-0003-3087-4297</orcidid><orcidid>https://orcid.org/0000-0003-3731-150X</orcidid><orcidid>https://orcid.org/0000-0002-7864-1081</orcidid><orcidid>https://orcid.org/0000-0002-3740-7539</orcidid><orcidid>https://orcid.org/0000-0002-4652-1376</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1932-6203 |
ispartof | PloS one, 2020-07, Vol.15 (7), p.e0235335-e0235335, Article 0235335 |
issn | 1932-6203 1932-6203 |
language | eng |
recordid | cdi_proquest_miscellaneous_2421122768 |
source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; Web of Science - Science Citation Index Expanded - 2020<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; PubMed Central; Free Full-Text Journals in Chemistry |
subjects | Antioxidants Biology and Life Sciences Blood Blood cells Cardiology and cardiovascular system Cardiovascular disease Cellular Biology Complications Damage accumulation Damage detection Deformability Diabetes Diabetes mellitus Diabetes research Endothelial cells Enzymatic activity Erythrocytes Formability Glucose Glycosylation Health aspects Hematology Hemoglobin Human health and pathology Hyperglycemia Impact damage Incubation Life Sciences Medicine and Health Sciences Mortality Multidisciplinary Sciences Oxidative stress Oxygen Phagocytosis Phosphatidylserine Physical Sciences Proteins Reactive oxygen species Red blood cells Science & Technology Science & Technology - Other Topics Structure |
title | Enhanced oxidative stress and damage in glycated erythrocytes |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-05T22%3A42%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enhanced%20oxidative%20stress%20and%20damage%20in%20glycated%20erythrocytes&rft.jtitle=PloS%20one&rft.au=Turpin,%20Chloe&rft.date=2020-07-06&rft.volume=15&rft.issue=7&rft.spage=e0235335&rft.epage=e0235335&rft.pages=e0235335-e0235335&rft.artnum=0235335&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0235335&rft_dat=%3Cgale_proqu%3EA628712528%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2420593559&rft_id=info:pmid/32628695&rft_galeid=A628712528&rft_doaj_id=oai_doaj_org_article_929c6c7e370b414ab4a6d762d4f7612c&rfr_iscdi=true |