Enhanced oxidative stress and damage in glycated erythrocytes

Diabetes is associated with a dramatic mortality rate due to its vascular complications. Chronic hyperglycemia in diabetes leads to enhanced glycation of erythrocytes and oxidative stress. Even though erythrocytes play a determining role in vascular complications, very little is known about how eryt...

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Veröffentlicht in:PloS one 2020-07, Vol.15 (7), p.e0235335-e0235335, Article 0235335
Hauptverfasser: Turpin, Chloe, Catan, Aurelie, Guerin-Dubourg, Alexis, Debussche, Xavier, Bravo, Susana B., Alvarez, Ezequiel, Van den Elsen, Jean, Meilhac, Olivier, Rondeau, Philippe, Bourdon, Emmanuel
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creator Turpin, Chloe
Catan, Aurelie
Guerin-Dubourg, Alexis
Debussche, Xavier
Bravo, Susana B.
Alvarez, Ezequiel
Van den Elsen, Jean
Meilhac, Olivier
Rondeau, Philippe
Bourdon, Emmanuel
description Diabetes is associated with a dramatic mortality rate due to its vascular complications. Chronic hyperglycemia in diabetes leads to enhanced glycation of erythrocytes and oxidative stress. Even though erythrocytes play a determining role in vascular complications, very little is known about how erythrocyte structure and functionality can be affected by glycation. Our objective was to decipher the impact of glycation on erythrocyte structure, oxidative stress parameters and capacity to interact with cultured human endothelial cells.In vitroglycated erythrocytes were prepared following incubation in the presence of different concentrations of glucose. To get insight into thein vivorelevance of our results, we compared these data to those obtained using red blood cells purified from diabetics or non-diabetics. We measured erythrocyte deformability, susceptibility to hemolysis, reactive oxygen species production and oxidative damage accumulation. Altered structures, redox status and oxidative modifications were increased in glycated erythrocytes. These modifications were associated with reduced antioxidant defence mediated by enzymatic activity. Enhanced erythrocyte phagocytosis by endothelial cells was observed when cultured with glycated erythrocytes, which was associated with increased levels of phosphatidylserine-likely as a result of an eryptosis phenomenon triggered by the hyperglycemic treatment. Most types of oxidative damage identified inin vitroglycated erythrocytes were also observed in red blood cells isolated from diabetics. These results bring new insights into the impact of glycation on erythrocyte structure, oxidative damage and their capacity to interact with endothelial cells, with a possible relevance to diabetes.
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Chronic hyperglycemia in diabetes leads to enhanced glycation of erythrocytes and oxidative stress. Even though erythrocytes play a determining role in vascular complications, very little is known about how erythrocyte structure and functionality can be affected by glycation. Our objective was to decipher the impact of glycation on erythrocyte structure, oxidative stress parameters and capacity to interact with cultured human endothelial cells.In vitroglycated erythrocytes were prepared following incubation in the presence of different concentrations of glucose. To get insight into thein vivorelevance of our results, we compared these data to those obtained using red blood cells purified from diabetics or non-diabetics. We measured erythrocyte deformability, susceptibility to hemolysis, reactive oxygen species production and oxidative damage accumulation. Altered structures, redox status and oxidative modifications were increased in glycated erythrocytes. 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Chronic hyperglycemia in diabetes leads to enhanced glycation of erythrocytes and oxidative stress. Even though erythrocytes play a determining role in vascular complications, very little is known about how erythrocyte structure and functionality can be affected by glycation. Our objective was to decipher the impact of glycation on erythrocyte structure, oxidative stress parameters and capacity to interact with cultured human endothelial cells.In vitroglycated erythrocytes were prepared following incubation in the presence of different concentrations of glucose. To get insight into thein vivorelevance of our results, we compared these data to those obtained using red blood cells purified from diabetics or non-diabetics. We measured erythrocyte deformability, susceptibility to hemolysis, reactive oxygen species production and oxidative damage accumulation. Altered structures, redox status and oxidative modifications were increased in glycated erythrocytes. These modifications were associated with reduced antioxidant defence mediated by enzymatic activity. Enhanced erythrocyte phagocytosis by endothelial cells was observed when cultured with glycated erythrocytes, which was associated with increased levels of phosphatidylserine-likely as a result of an eryptosis phenomenon triggered by the hyperglycemic treatment. Most types of oxidative damage identified inin vitroglycated erythrocytes were also observed in red blood cells isolated from diabetics. 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source DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); Web of Science - Science Citation Index Expanded - 2020<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; PubMed Central; Free Full-Text Journals in Chemistry
subjects Antioxidants
Biology and Life Sciences
Blood
Blood cells
Cardiology and cardiovascular system
Cardiovascular disease
Cellular Biology
Complications
Damage accumulation
Damage detection
Deformability
Diabetes
Diabetes mellitus
Diabetes research
Endothelial cells
Enzymatic activity
Erythrocytes
Formability
Glucose
Glycosylation
Health aspects
Hematology
Hemoglobin
Human health and pathology
Hyperglycemia
Impact damage
Incubation
Life Sciences
Medicine and Health Sciences
Mortality
Multidisciplinary Sciences
Oxidative stress
Oxygen
Phagocytosis
Phosphatidylserine
Physical Sciences
Proteins
Reactive oxygen species
Red blood cells
Science & Technology
Science & Technology - Other Topics
Structure
title Enhanced oxidative stress and damage in glycated erythrocytes
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