Comparative transcriptomic and physiological analyses of contrasting hybrid cultivars ND476 and ZX978 identify important differentially expressed genes and pathways regulating drought stress tolerance in maize
Background Drought is the major abiotic stress factor that negatively influences growth and yield in cereal grain crops such as maize ( Zea mays L.). A multitude of genes and pathways tightly modulate plant growth, development and responses to environmental stresses including drought. Therefore, cro...
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| Veröffentlicht in: | Genes & genomics 2020-08, Vol.42 (8), p.937-955 |
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| container_title | Genes & genomics |
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| creator | Liu, Guo Zenda, Tinashe Liu, Songtao Wang, Xuan Jin, Hongyu Dong, Anyi Yang, Yatong Duan, Huijun |
| description | Background
Drought is the major abiotic stress factor that negatively influences growth and yield in cereal grain crops such as maize (
Zea mays
L.). A multitude of genes and pathways tightly modulate plant growth, development and responses to environmental stresses including drought. Therefore, crop breeding efforts for enhanced drought resistance require improved knowledge of plant drought responses.
Objective
Here, we sought to elucidate the molecular and physiological mechanisms underpinning maize drought stress tolerance.
Methods
We therefore applied a 12-day water-deficit stress treatment to maize plants of two contrasting (drought tolerant ND476 and drought sensitive ZX978) hybrid cultivars at the late vegetative (V12) growth stage and performed a large-scale RNA sequencing (RNA-seq) transcriptome analysis of the leaf tissues.
Results
A comparative analysis of the two genotypes leaf transcriptomes and physiological parameters revealed the key differentially expressed genes (DEGs) and metabolic pathways that respond to drought in a genotype-specific manner. A total of 3114 DEGs were identified, with 21 DEGs being specifically expressed in tolerant genotype ND476 in response to drought stress. Of these, genes involved in secondary metabolites biosynthesis, transcription factor regulation, detoxification and stress defense were highly expressed in ND476. Physiological analysis results substantiated our RNA-seq data, with ND476 exhibiting better cell water retention, higher soluble protein content and guaiacol peroxidase activity, along with low lipid peroxidation extent than the sensitive cultivar ZX978 under drought conditions.
Conclusion
Our findings enrich the maize genetic resources and enhance our further understanding of the molecular mechanisms regulating drought stress tolerance in maize. Additionally, the DEGs screened in this study may provide a foundational basis for our future targeted cloning studies. |
| doi_str_mv | 10.1007/s13258-020-00962-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2420645280</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2425895709</sourcerecordid><originalsourceid>FETCH-LOGICAL-c352t-6d76a2249cc5cd7f77d1ec2ce5b00a9debcadd013dd3c5938ebae63a30375e973</originalsourceid><addsrcrecordid>eNp9kU-r1DAUxYsoODzfF3AVcOOmmj9t0yxl_AuP50ZB3JQ7yW0njzSpSeqzfku_kZkZQXDh3Vy4_M45IaeqnjL6glEqXyYmeNvXlNOaUtXxunlQ7ThVvFZciYfVjinZ1aqV7HF1ndIdLSNY0zVsV_3ah3mBCNl-R5Ij-KSjXXKYrSbgDVmOW7LBhclqcOUCbkuYSBiJDr7wKVs_keN2iNYQvbriAzGR29eN7M4GX78o2RNr0Gc7bsTOS4gZfCbGjiPG0xmc2wj-WCKmhIZM6EvCORzy8R62RCJOq4NzlIlhnY6ZpHzCSQ4Oy6s1EuvJDPYnPqkejeASXv_ZV9Xnt28-7d_XNx_ffdi_uqm1aHmuOyM74LxRWrfayFFKw1Bzje2BUlAGDxqMoUwYI3SrRI8HwE6AoEK2qKS4qp5ffJcYvq2Y8jDbpNE58BjWNPCG065peU8L-uwf9C6ssfzlmWr7Ug1VheIXSseQUsRxWKKdIW4Do8Op6OFS9FCKHs5FD00RiYsoFdhPGP9a_0f1Gyoesgc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2425895709</pqid></control><display><type>article</type><title>Comparative transcriptomic and physiological analyses of contrasting hybrid cultivars ND476 and ZX978 identify important differentially expressed genes and pathways regulating drought stress tolerance in maize</title><source>Springer Nature - Complete Springer Journals</source><creator>Liu, Guo ; Zenda, Tinashe ; Liu, Songtao ; Wang, Xuan ; Jin, Hongyu ; Dong, Anyi ; Yang, Yatong ; Duan, Huijun</creator><creatorcontrib>Liu, Guo ; Zenda, Tinashe ; Liu, Songtao ; Wang, Xuan ; Jin, Hongyu ; Dong, Anyi ; Yang, Yatong ; Duan, Huijun</creatorcontrib><description>Background
Drought is the major abiotic stress factor that negatively influences growth and yield in cereal grain crops such as maize (
Zea mays
L.). A multitude of genes and pathways tightly modulate plant growth, development and responses to environmental stresses including drought. Therefore, crop breeding efforts for enhanced drought resistance require improved knowledge of plant drought responses.
Objective
Here, we sought to elucidate the molecular and physiological mechanisms underpinning maize drought stress tolerance.
Methods
We therefore applied a 12-day water-deficit stress treatment to maize plants of two contrasting (drought tolerant ND476 and drought sensitive ZX978) hybrid cultivars at the late vegetative (V12) growth stage and performed a large-scale RNA sequencing (RNA-seq) transcriptome analysis of the leaf tissues.
Results
A comparative analysis of the two genotypes leaf transcriptomes and physiological parameters revealed the key differentially expressed genes (DEGs) and metabolic pathways that respond to drought in a genotype-specific manner. A total of 3114 DEGs were identified, with 21 DEGs being specifically expressed in tolerant genotype ND476 in response to drought stress. Of these, genes involved in secondary metabolites biosynthesis, transcription factor regulation, detoxification and stress defense were highly expressed in ND476. Physiological analysis results substantiated our RNA-seq data, with ND476 exhibiting better cell water retention, higher soluble protein content and guaiacol peroxidase activity, along with low lipid peroxidation extent than the sensitive cultivar ZX978 under drought conditions.
Conclusion
Our findings enrich the maize genetic resources and enhance our further understanding of the molecular mechanisms regulating drought stress tolerance in maize. Additionally, the DEGs screened in this study may provide a foundational basis for our future targeted cloning studies.</description><identifier>ISSN: 1976-9571</identifier><identifier>EISSN: 2092-9293</identifier><identifier>DOI: 10.1007/s13258-020-00962-4</identifier><language>eng</language><publisher>Singapore: Springer Singapore</publisher><subject>Abiotic factors ; Animal Genetics and Genomics ; Biomedical and Life Sciences ; Comparative analysis ; Cultivars ; Detoxification ; Drought ; Drought resistance ; Gene expression ; Gene regulation ; Genetic resources ; Genotypes ; Growth stage ; Guaiacol ; Human Genetics ; Hydration ; Leaves ; Life Sciences ; Lipid peroxidation ; Metabolic pathways ; Microbial Genetics and Genomics ; Molecular modelling ; Physiology ; Plant breeding ; Plant Genetics and Genomics ; Research Article ; Ribonucleic acid ; RNA ; Secondary metabolites ; Zea mays</subject><ispartof>Genes & genomics, 2020-08, Vol.42 (8), p.937-955</ispartof><rights>The Genetics Society of Korea 2020</rights><rights>The Genetics Society of Korea 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-6d76a2249cc5cd7f77d1ec2ce5b00a9debcadd013dd3c5938ebae63a30375e973</citedby><cites>FETCH-LOGICAL-c352t-6d76a2249cc5cd7f77d1ec2ce5b00a9debcadd013dd3c5938ebae63a30375e973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13258-020-00962-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13258-020-00962-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Liu, Guo</creatorcontrib><creatorcontrib>Zenda, Tinashe</creatorcontrib><creatorcontrib>Liu, Songtao</creatorcontrib><creatorcontrib>Wang, Xuan</creatorcontrib><creatorcontrib>Jin, Hongyu</creatorcontrib><creatorcontrib>Dong, Anyi</creatorcontrib><creatorcontrib>Yang, Yatong</creatorcontrib><creatorcontrib>Duan, Huijun</creatorcontrib><title>Comparative transcriptomic and physiological analyses of contrasting hybrid cultivars ND476 and ZX978 identify important differentially expressed genes and pathways regulating drought stress tolerance in maize</title><title>Genes & genomics</title><addtitle>Genes Genom</addtitle><description>Background
Drought is the major abiotic stress factor that negatively influences growth and yield in cereal grain crops such as maize (
Zea mays
L.). A multitude of genes and pathways tightly modulate plant growth, development and responses to environmental stresses including drought. Therefore, crop breeding efforts for enhanced drought resistance require improved knowledge of plant drought responses.
Objective
Here, we sought to elucidate the molecular and physiological mechanisms underpinning maize drought stress tolerance.
Methods
We therefore applied a 12-day water-deficit stress treatment to maize plants of two contrasting (drought tolerant ND476 and drought sensitive ZX978) hybrid cultivars at the late vegetative (V12) growth stage and performed a large-scale RNA sequencing (RNA-seq) transcriptome analysis of the leaf tissues.
Results
A comparative analysis of the two genotypes leaf transcriptomes and physiological parameters revealed the key differentially expressed genes (DEGs) and metabolic pathways that respond to drought in a genotype-specific manner. A total of 3114 DEGs were identified, with 21 DEGs being specifically expressed in tolerant genotype ND476 in response to drought stress. Of these, genes involved in secondary metabolites biosynthesis, transcription factor regulation, detoxification and stress defense were highly expressed in ND476. Physiological analysis results substantiated our RNA-seq data, with ND476 exhibiting better cell water retention, higher soluble protein content and guaiacol peroxidase activity, along with low lipid peroxidation extent than the sensitive cultivar ZX978 under drought conditions.
Conclusion
Our findings enrich the maize genetic resources and enhance our further understanding of the molecular mechanisms regulating drought stress tolerance in maize. Additionally, the DEGs screened in this study may provide a foundational basis for our future targeted cloning studies.</description><subject>Abiotic factors</subject><subject>Animal Genetics and Genomics</subject><subject>Biomedical and Life Sciences</subject><subject>Comparative analysis</subject><subject>Cultivars</subject><subject>Detoxification</subject><subject>Drought</subject><subject>Drought resistance</subject><subject>Gene expression</subject><subject>Gene regulation</subject><subject>Genetic resources</subject><subject>Genotypes</subject><subject>Growth stage</subject><subject>Guaiacol</subject><subject>Human Genetics</subject><subject>Hydration</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Lipid peroxidation</subject><subject>Metabolic pathways</subject><subject>Microbial Genetics and Genomics</subject><subject>Molecular modelling</subject><subject>Physiology</subject><subject>Plant breeding</subject><subject>Plant Genetics and Genomics</subject><subject>Research Article</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Secondary metabolites</subject><subject>Zea mays</subject><issn>1976-9571</issn><issn>2092-9293</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kU-r1DAUxYsoODzfF3AVcOOmmj9t0yxl_AuP50ZB3JQ7yW0njzSpSeqzfku_kZkZQXDh3Vy4_M45IaeqnjL6glEqXyYmeNvXlNOaUtXxunlQ7ThVvFZciYfVjinZ1aqV7HF1ndIdLSNY0zVsV_3ah3mBCNl-R5Ij-KSjXXKYrSbgDVmOW7LBhclqcOUCbkuYSBiJDr7wKVs_keN2iNYQvbriAzGR29eN7M4GX78o2RNr0Gc7bsTOS4gZfCbGjiPG0xmc2wj-WCKmhIZM6EvCORzy8R62RCJOq4NzlIlhnY6ZpHzCSQ4Oy6s1EuvJDPYnPqkejeASXv_ZV9Xnt28-7d_XNx_ffdi_uqm1aHmuOyM74LxRWrfayFFKw1Bzje2BUlAGDxqMoUwYI3SrRI8HwE6AoEK2qKS4qp5ffJcYvq2Y8jDbpNE58BjWNPCG065peU8L-uwf9C6ssfzlmWr7Ug1VheIXSseQUsRxWKKdIW4Do8Op6OFS9FCKHs5FD00RiYsoFdhPGP9a_0f1Gyoesgc</recordid><startdate>20200801</startdate><enddate>20200801</enddate><creator>Liu, Guo</creator><creator>Zenda, Tinashe</creator><creator>Liu, Songtao</creator><creator>Wang, Xuan</creator><creator>Jin, Hongyu</creator><creator>Dong, Anyi</creator><creator>Yang, Yatong</creator><creator>Duan, Huijun</creator><general>Springer Singapore</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20200801</creationdate><title>Comparative transcriptomic and physiological analyses of contrasting hybrid cultivars ND476 and ZX978 identify important differentially expressed genes and pathways regulating drought stress tolerance in maize</title><author>Liu, Guo ; Zenda, Tinashe ; Liu, Songtao ; Wang, Xuan ; Jin, Hongyu ; Dong, Anyi ; Yang, Yatong ; Duan, Huijun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-6d76a2249cc5cd7f77d1ec2ce5b00a9debcadd013dd3c5938ebae63a30375e973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Abiotic factors</topic><topic>Animal Genetics and Genomics</topic><topic>Biomedical and Life Sciences</topic><topic>Comparative analysis</topic><topic>Cultivars</topic><topic>Detoxification</topic><topic>Drought</topic><topic>Drought resistance</topic><topic>Gene expression</topic><topic>Gene regulation</topic><topic>Genetic resources</topic><topic>Genotypes</topic><topic>Growth stage</topic><topic>Guaiacol</topic><topic>Human Genetics</topic><topic>Hydration</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Lipid peroxidation</topic><topic>Metabolic pathways</topic><topic>Microbial Genetics and Genomics</topic><topic>Molecular modelling</topic><topic>Physiology</topic><topic>Plant breeding</topic><topic>Plant Genetics and Genomics</topic><topic>Research Article</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Secondary metabolites</topic><topic>Zea mays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Guo</creatorcontrib><creatorcontrib>Zenda, Tinashe</creatorcontrib><creatorcontrib>Liu, Songtao</creatorcontrib><creatorcontrib>Wang, Xuan</creatorcontrib><creatorcontrib>Jin, Hongyu</creatorcontrib><creatorcontrib>Dong, Anyi</creatorcontrib><creatorcontrib>Yang, Yatong</creatorcontrib><creatorcontrib>Duan, Huijun</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Genes & genomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Guo</au><au>Zenda, Tinashe</au><au>Liu, Songtao</au><au>Wang, Xuan</au><au>Jin, Hongyu</au><au>Dong, Anyi</au><au>Yang, Yatong</au><au>Duan, Huijun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative transcriptomic and physiological analyses of contrasting hybrid cultivars ND476 and ZX978 identify important differentially expressed genes and pathways regulating drought stress tolerance in maize</atitle><jtitle>Genes & genomics</jtitle><stitle>Genes Genom</stitle><date>2020-08-01</date><risdate>2020</risdate><volume>42</volume><issue>8</issue><spage>937</spage><epage>955</epage><pages>937-955</pages><issn>1976-9571</issn><eissn>2092-9293</eissn><abstract>Background
Drought is the major abiotic stress factor that negatively influences growth and yield in cereal grain crops such as maize (
Zea mays
L.). A multitude of genes and pathways tightly modulate plant growth, development and responses to environmental stresses including drought. Therefore, crop breeding efforts for enhanced drought resistance require improved knowledge of plant drought responses.
Objective
Here, we sought to elucidate the molecular and physiological mechanisms underpinning maize drought stress tolerance.
Methods
We therefore applied a 12-day water-deficit stress treatment to maize plants of two contrasting (drought tolerant ND476 and drought sensitive ZX978) hybrid cultivars at the late vegetative (V12) growth stage and performed a large-scale RNA sequencing (RNA-seq) transcriptome analysis of the leaf tissues.
Results
A comparative analysis of the two genotypes leaf transcriptomes and physiological parameters revealed the key differentially expressed genes (DEGs) and metabolic pathways that respond to drought in a genotype-specific manner. A total of 3114 DEGs were identified, with 21 DEGs being specifically expressed in tolerant genotype ND476 in response to drought stress. Of these, genes involved in secondary metabolites biosynthesis, transcription factor regulation, detoxification and stress defense were highly expressed in ND476. Physiological analysis results substantiated our RNA-seq data, with ND476 exhibiting better cell water retention, higher soluble protein content and guaiacol peroxidase activity, along with low lipid peroxidation extent than the sensitive cultivar ZX978 under drought conditions.
Conclusion
Our findings enrich the maize genetic resources and enhance our further understanding of the molecular mechanisms regulating drought stress tolerance in maize. Additionally, the DEGs screened in this study may provide a foundational basis for our future targeted cloning studies.</abstract><cop>Singapore</cop><pub>Springer Singapore</pub><doi>10.1007/s13258-020-00962-4</doi><tpages>19</tpages></addata></record> |
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| ispartof | Genes & genomics, 2020-08, Vol.42 (8), p.937-955 |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Abiotic factors Animal Genetics and Genomics Biomedical and Life Sciences Comparative analysis Cultivars Detoxification Drought Drought resistance Gene expression Gene regulation Genetic resources Genotypes Growth stage Guaiacol Human Genetics Hydration Leaves Life Sciences Lipid peroxidation Metabolic pathways Microbial Genetics and Genomics Molecular modelling Physiology Plant breeding Plant Genetics and Genomics Research Article Ribonucleic acid RNA Secondary metabolites Zea mays |
| title | Comparative transcriptomic and physiological analyses of contrasting hybrid cultivars ND476 and ZX978 identify important differentially expressed genes and pathways regulating drought stress tolerance in maize |
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