ATR-IR and EPR spectroscopy for following the membrane restoration of isolated cortical synaptosomes in aluminium-induced Alzheimer’s disease – Like rat model
[Display omitted] •ATR-IR biophysical changes in isolated rat cortex synaptic membranes.•The potential therapeutic effect of Lepedium sativum (LS) in treating AD.•AlCl3 experienced oxidative damage that direct affects synaptic membrane lipids.•Al induces imbalance of Ca2+ homeostasis, brain atrophy...
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creator | Ahmed, Gehan A.-R. Khalil, Safaa K.H. Hotaby, W. El Abbas, Lamyaa Farrag, Abdel Razik H. Aal, Wafaa E. Abdel Sherif, Hadeer H.A. Abdel-Rahman, Engy A. Saber, Saber H. Hassan, Mahmoud Hassan, Mohamed H. Balgoon, Maha Qusti, Safaa Kotb, Mamdooh Ali, Sameh S. |
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•ATR-IR biophysical changes in isolated rat cortex synaptic membranes.•The potential therapeutic effect of Lepedium sativum (LS) in treating AD.•AlCl3 experienced oxidative damage that direct affects synaptic membrane lipids.•Al induces imbalance of Ca2+ homeostasis, brain atrophy (MRI) & animal behavior.•NADPH oxidase activation leads to OS & alterations in membrane physical properties.
Synaptosomal membrane peroxidation and alteration in its biophysical properties are associated with Aluminium (Al) toxicity that may lead to cognitive dysfunction and Alzheimer’s disease (AD) like pathogenesis. Here we investigated the therapeutic potential of Lepedium sativum (LS) as a natural anti-inflammatory, antioxidant and as acetyl cholinesterase inhibitor in treating Al induced AD-like in rat model. We utilized ATR-IR spectroscopy to follow the restoration in the damaged membrane structure of isolated rat cortical synaptosomes and its biophysical properties, electron paramagnetic resonance (EPR) spin trapping to follow NADPH oxidase activity (NOX), and EPR spin labelling in response to LS treatment after Al intoxication. We measured the concentration of Ca2+ ions in rat cortical tissue by inductively coupled plasma (ICP), the brain atrophy/curing and hydrocephalus by magnetic resonance imaging (MRI) besides light microscope histopathology. Our results revealed significant increase in synaptosomal membrane rgidification, order, lipid packing, reactive oxygen species (ROS) production and Ca2+ ion concentration as a result of Al intoxication. The dramatic increase in Ca2+ ion concentration detected in AD group associated with the increase in synaptic membrane polarity and EPR-detected order S-parameter suggest that release of synaptic vesicles into synaptic cleft might be hindered. LS treatment reversed these changes in synaptic membranes, and rescued an observed deficit in the exploratory behaviour of AD group. Our results also strongly suggest that the synaptosomal membrane phospholipids that underwent free radical attacks mediated by AlCl3, due to greater NOX activity, was prevented in the LS group. The results of ATR-IR and EPR spectroscopic techniques recommend LS as a promising therapeutic agent against synaptic membrane alterations opening a new window for AD drug developers. |
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•ATR-IR biophysical changes in isolated rat cortex synaptic membranes.•The potential therapeutic effect of Lepedium sativum (LS) in treating AD.•AlCl3 experienced oxidative damage that direct affects synaptic membrane lipids.•Al induces imbalance of Ca2+ homeostasis, brain atrophy (MRI) & animal behavior.•NADPH oxidase activation leads to OS & alterations in membrane physical properties.
Synaptosomal membrane peroxidation and alteration in its biophysical properties are associated with Aluminium (Al) toxicity that may lead to cognitive dysfunction and Alzheimer’s disease (AD) like pathogenesis. Here we investigated the therapeutic potential of Lepedium sativum (LS) as a natural anti-inflammatory, antioxidant and as acetyl cholinesterase inhibitor in treating Al induced AD-like in rat model. We utilized ATR-IR spectroscopy to follow the restoration in the damaged membrane structure of isolated rat cortical synaptosomes and its biophysical properties, electron paramagnetic resonance (EPR) spin trapping to follow NADPH oxidase activity (NOX), and EPR spin labelling in response to LS treatment after Al intoxication. We measured the concentration of Ca2+ ions in rat cortical tissue by inductively coupled plasma (ICP), the brain atrophy/curing and hydrocephalus by magnetic resonance imaging (MRI) besides light microscope histopathology. Our results revealed significant increase in synaptosomal membrane rgidification, order, lipid packing, reactive oxygen species (ROS) production and Ca2+ ion concentration as a result of Al intoxication. The dramatic increase in Ca2+ ion concentration detected in AD group associated with the increase in synaptic membrane polarity and EPR-detected order S-parameter suggest that release of synaptic vesicles into synaptic cleft might be hindered. LS treatment reversed these changes in synaptic membranes, and rescued an observed deficit in the exploratory behaviour of AD group. Our results also strongly suggest that the synaptosomal membrane phospholipids that underwent free radical attacks mediated by AlCl3, due to greater NOX activity, was prevented in the LS group. The results of ATR-IR and EPR spectroscopic techniques recommend LS as a promising therapeutic agent against synaptic membrane alterations opening a new window for AD drug developers.</description><identifier>ISSN: 0009-3084</identifier><identifier>EISSN: 1873-2941</identifier><identifier>DOI: 10.1016/j.chemphyslip.2020.104931</identifier><identifier>PMID: 32619464</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Aluminum Chloride - administration & dosage ; Alzheimer Disease - chemically induced ; Alzheimer Disease - metabolism ; Alzheimer Disease - pathology ; Alzheimer's disease treatment ; Animals ; ATR-IR ; Cerebral Cortex - metabolism ; Cortex synaptosomes ; Disease Models, Animal ; Electron Spin Resonance Spectroscopy ; EPR ; Injections, Intraperitoneal ; Lepidium sativum ; Lipid peroxidation ; Male ; Membrane biophysical structure ; Rats ; Rats, Wistar ; Spectrophotometry, Infrared ; Synaptosomes - metabolism</subject><ispartof>Chemistry and physics of lipids, 2020-09, Vol.231, p.104931-104931, Article 104931</ispartof><rights>2020 Elsevier B.V.</rights><rights>Copyright © 2020 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-ef21bf0620a87474fff3f9b3344a410ad8db1b358cbee2b572c64aba98ce838c3</citedby><cites>FETCH-LOGICAL-c377t-ef21bf0620a87474fff3f9b3344a410ad8db1b358cbee2b572c64aba98ce838c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.chemphyslip.2020.104931$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32619464$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ahmed, Gehan A.-R.</creatorcontrib><creatorcontrib>Khalil, Safaa K.H.</creatorcontrib><creatorcontrib>Hotaby, W. El</creatorcontrib><creatorcontrib>Abbas, Lamyaa</creatorcontrib><creatorcontrib>Farrag, Abdel Razik H.</creatorcontrib><creatorcontrib>Aal, Wafaa E. Abdel</creatorcontrib><creatorcontrib>Sherif, Hadeer H.A.</creatorcontrib><creatorcontrib>Abdel-Rahman, Engy A.</creatorcontrib><creatorcontrib>Saber, Saber H.</creatorcontrib><creatorcontrib>Hassan, Mahmoud</creatorcontrib><creatorcontrib>Hassan, Mohamed H.</creatorcontrib><creatorcontrib>Balgoon, Maha</creatorcontrib><creatorcontrib>Qusti, Safaa</creatorcontrib><creatorcontrib>Kotb, Mamdooh</creatorcontrib><creatorcontrib>Ali, Sameh S.</creatorcontrib><title>ATR-IR and EPR spectroscopy for following the membrane restoration of isolated cortical synaptosomes in aluminium-induced Alzheimer’s disease – Like rat model</title><title>Chemistry and physics of lipids</title><addtitle>Chem Phys Lipids</addtitle><description>[Display omitted]
•ATR-IR biophysical changes in isolated rat cortex synaptic membranes.•The potential therapeutic effect of Lepedium sativum (LS) in treating AD.•AlCl3 experienced oxidative damage that direct affects synaptic membrane lipids.•Al induces imbalance of Ca2+ homeostasis, brain atrophy (MRI) & animal behavior.•NADPH oxidase activation leads to OS & alterations in membrane physical properties.
Synaptosomal membrane peroxidation and alteration in its biophysical properties are associated with Aluminium (Al) toxicity that may lead to cognitive dysfunction and Alzheimer’s disease (AD) like pathogenesis. Here we investigated the therapeutic potential of Lepedium sativum (LS) as a natural anti-inflammatory, antioxidant and as acetyl cholinesterase inhibitor in treating Al induced AD-like in rat model. We utilized ATR-IR spectroscopy to follow the restoration in the damaged membrane structure of isolated rat cortical synaptosomes and its biophysical properties, electron paramagnetic resonance (EPR) spin trapping to follow NADPH oxidase activity (NOX), and EPR spin labelling in response to LS treatment after Al intoxication. We measured the concentration of Ca2+ ions in rat cortical tissue by inductively coupled plasma (ICP), the brain atrophy/curing and hydrocephalus by magnetic resonance imaging (MRI) besides light microscope histopathology. Our results revealed significant increase in synaptosomal membrane rgidification, order, lipid packing, reactive oxygen species (ROS) production and Ca2+ ion concentration as a result of Al intoxication. The dramatic increase in Ca2+ ion concentration detected in AD group associated with the increase in synaptic membrane polarity and EPR-detected order S-parameter suggest that release of synaptic vesicles into synaptic cleft might be hindered. LS treatment reversed these changes in synaptic membranes, and rescued an observed deficit in the exploratory behaviour of AD group. Our results also strongly suggest that the synaptosomal membrane phospholipids that underwent free radical attacks mediated by AlCl3, due to greater NOX activity, was prevented in the LS group. The results of ATR-IR and EPR spectroscopic techniques recommend LS as a promising therapeutic agent against synaptic membrane alterations opening a new window for AD drug developers.</description><subject>Aluminum Chloride - administration & dosage</subject><subject>Alzheimer Disease - chemically induced</subject><subject>Alzheimer Disease - metabolism</subject><subject>Alzheimer Disease - pathology</subject><subject>Alzheimer's disease treatment</subject><subject>Animals</subject><subject>ATR-IR</subject><subject>Cerebral Cortex - metabolism</subject><subject>Cortex synaptosomes</subject><subject>Disease Models, Animal</subject><subject>Electron Spin Resonance Spectroscopy</subject><subject>EPR</subject><subject>Injections, Intraperitoneal</subject><subject>Lepidium sativum</subject><subject>Lipid peroxidation</subject><subject>Male</subject><subject>Membrane biophysical structure</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Spectrophotometry, Infrared</subject><subject>Synaptosomes - metabolism</subject><issn>0009-3084</issn><issn>1873-2941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcuOEzEQRS0EYsLALyCzY9PBr_RjGUUDjBQJFA1ry-0uEwc_Grt7UFjNP7Biy6fNl-AoA2LJwiq5dKuubh2EXlGypITWbw5LvQc_7o_Z2XHJCDv1RcfpI7SgbcMr1gn6GC0IIV3FSSsu0LOcD-VLViv6FF1wVtNO1GKBfq1vdtX1Dqsw4KuPO5xH0FOKWcfxiE1M5TkXv9nwGU97wB58n1QAnCBPManJxoCjwTZHpyYYsI5pslo5nI9BjVPM0UPGNmDlZm-DnX1lwzDrIl2773uwHtL93c-MB5tBZcD3dz_w1n4pDmrCPg7gnqMnRrkMLx7qJfr09upm877afnh3vVlvK82bZqrAMNobUjOi2kY0whjDTddzLoQSlKihHXra81WrewDWrxqma6F61bUaWt5qfolen_eOKX6dSz7pbdbgXMkb5yyZYIQK2lFSpN1ZqsupcgIjx2S9SkdJiTwhkgf5DyJ5QiTPiMrsywebufcw_J38w6QINmcBlLC3FpLM2kIoJ7OpwJFDtP9h8xvMQ65N</recordid><startdate>202009</startdate><enddate>202009</enddate><creator>Ahmed, Gehan A.-R.</creator><creator>Khalil, Safaa K.H.</creator><creator>Hotaby, W. El</creator><creator>Abbas, Lamyaa</creator><creator>Farrag, Abdel Razik H.</creator><creator>Aal, Wafaa E. Abdel</creator><creator>Sherif, Hadeer H.A.</creator><creator>Abdel-Rahman, Engy A.</creator><creator>Saber, Saber H.</creator><creator>Hassan, Mahmoud</creator><creator>Hassan, Mohamed H.</creator><creator>Balgoon, Maha</creator><creator>Qusti, Safaa</creator><creator>Kotb, Mamdooh</creator><creator>Ali, Sameh S.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202009</creationdate><title>ATR-IR and EPR spectroscopy for following the membrane restoration of isolated cortical synaptosomes in aluminium-induced Alzheimer’s disease – Like rat model</title><author>Ahmed, Gehan A.-R. ; Khalil, Safaa K.H. ; Hotaby, W. El ; Abbas, Lamyaa ; Farrag, Abdel Razik H. ; Aal, Wafaa E. Abdel ; Sherif, Hadeer H.A. ; Abdel-Rahman, Engy A. ; Saber, Saber H. ; Hassan, Mahmoud ; Hassan, Mohamed H. ; Balgoon, Maha ; Qusti, Safaa ; Kotb, Mamdooh ; Ali, Sameh S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-ef21bf0620a87474fff3f9b3344a410ad8db1b358cbee2b572c64aba98ce838c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aluminum Chloride - administration & dosage</topic><topic>Alzheimer Disease - chemically induced</topic><topic>Alzheimer Disease - metabolism</topic><topic>Alzheimer Disease - pathology</topic><topic>Alzheimer's disease treatment</topic><topic>Animals</topic><topic>ATR-IR</topic><topic>Cerebral Cortex - metabolism</topic><topic>Cortex synaptosomes</topic><topic>Disease Models, Animal</topic><topic>Electron Spin Resonance Spectroscopy</topic><topic>EPR</topic><topic>Injections, Intraperitoneal</topic><topic>Lepidium sativum</topic><topic>Lipid peroxidation</topic><topic>Male</topic><topic>Membrane biophysical structure</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Spectrophotometry, Infrared</topic><topic>Synaptosomes - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ahmed, Gehan A.-R.</creatorcontrib><creatorcontrib>Khalil, Safaa K.H.</creatorcontrib><creatorcontrib>Hotaby, W. El</creatorcontrib><creatorcontrib>Abbas, Lamyaa</creatorcontrib><creatorcontrib>Farrag, Abdel Razik H.</creatorcontrib><creatorcontrib>Aal, Wafaa E. Abdel</creatorcontrib><creatorcontrib>Sherif, Hadeer H.A.</creatorcontrib><creatorcontrib>Abdel-Rahman, Engy A.</creatorcontrib><creatorcontrib>Saber, Saber H.</creatorcontrib><creatorcontrib>Hassan, Mahmoud</creatorcontrib><creatorcontrib>Hassan, Mohamed H.</creatorcontrib><creatorcontrib>Balgoon, Maha</creatorcontrib><creatorcontrib>Qusti, Safaa</creatorcontrib><creatorcontrib>Kotb, Mamdooh</creatorcontrib><creatorcontrib>Ali, Sameh S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chemistry and physics of lipids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ahmed, Gehan A.-R.</au><au>Khalil, Safaa K.H.</au><au>Hotaby, W. El</au><au>Abbas, Lamyaa</au><au>Farrag, Abdel Razik H.</au><au>Aal, Wafaa E. Abdel</au><au>Sherif, Hadeer H.A.</au><au>Abdel-Rahman, Engy A.</au><au>Saber, Saber H.</au><au>Hassan, Mahmoud</au><au>Hassan, Mohamed H.</au><au>Balgoon, Maha</au><au>Qusti, Safaa</au><au>Kotb, Mamdooh</au><au>Ali, Sameh S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ATR-IR and EPR spectroscopy for following the membrane restoration of isolated cortical synaptosomes in aluminium-induced Alzheimer’s disease – Like rat model</atitle><jtitle>Chemistry and physics of lipids</jtitle><addtitle>Chem Phys Lipids</addtitle><date>2020-09</date><risdate>2020</risdate><volume>231</volume><spage>104931</spage><epage>104931</epage><pages>104931-104931</pages><artnum>104931</artnum><issn>0009-3084</issn><eissn>1873-2941</eissn><abstract>[Display omitted]
•ATR-IR biophysical changes in isolated rat cortex synaptic membranes.•The potential therapeutic effect of Lepedium sativum (LS) in treating AD.•AlCl3 experienced oxidative damage that direct affects synaptic membrane lipids.•Al induces imbalance of Ca2+ homeostasis, brain atrophy (MRI) & animal behavior.•NADPH oxidase activation leads to OS & alterations in membrane physical properties.
Synaptosomal membrane peroxidation and alteration in its biophysical properties are associated with Aluminium (Al) toxicity that may lead to cognitive dysfunction and Alzheimer’s disease (AD) like pathogenesis. Here we investigated the therapeutic potential of Lepedium sativum (LS) as a natural anti-inflammatory, antioxidant and as acetyl cholinesterase inhibitor in treating Al induced AD-like in rat model. We utilized ATR-IR spectroscopy to follow the restoration in the damaged membrane structure of isolated rat cortical synaptosomes and its biophysical properties, electron paramagnetic resonance (EPR) spin trapping to follow NADPH oxidase activity (NOX), and EPR spin labelling in response to LS treatment after Al intoxication. We measured the concentration of Ca2+ ions in rat cortical tissue by inductively coupled plasma (ICP), the brain atrophy/curing and hydrocephalus by magnetic resonance imaging (MRI) besides light microscope histopathology. Our results revealed significant increase in synaptosomal membrane rgidification, order, lipid packing, reactive oxygen species (ROS) production and Ca2+ ion concentration as a result of Al intoxication. The dramatic increase in Ca2+ ion concentration detected in AD group associated with the increase in synaptic membrane polarity and EPR-detected order S-parameter suggest that release of synaptic vesicles into synaptic cleft might be hindered. LS treatment reversed these changes in synaptic membranes, and rescued an observed deficit in the exploratory behaviour of AD group. Our results also strongly suggest that the synaptosomal membrane phospholipids that underwent free radical attacks mediated by AlCl3, due to greater NOX activity, was prevented in the LS group. The results of ATR-IR and EPR spectroscopic techniques recommend LS as a promising therapeutic agent against synaptic membrane alterations opening a new window for AD drug developers.</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>32619464</pmid><doi>10.1016/j.chemphyslip.2020.104931</doi><tpages>1</tpages></addata></record> |
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subjects | Aluminum Chloride - administration & dosage Alzheimer Disease - chemically induced Alzheimer Disease - metabolism Alzheimer Disease - pathology Alzheimer's disease treatment Animals ATR-IR Cerebral Cortex - metabolism Cortex synaptosomes Disease Models, Animal Electron Spin Resonance Spectroscopy EPR Injections, Intraperitoneal Lepidium sativum Lipid peroxidation Male Membrane biophysical structure Rats Rats, Wistar Spectrophotometry, Infrared Synaptosomes - metabolism |
title | ATR-IR and EPR spectroscopy for following the membrane restoration of isolated cortical synaptosomes in aluminium-induced Alzheimer’s disease – Like rat model |
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