Actinokineospora pegani sp. nov., an endophytic actinomycete isolated from the surface-sterilized root of Peganum harmala L
A novel Gram-positive, aerobic and motile endophytic actinomycete, designated TRM 65233 , was isolated from the root of L. collected from Xinjiang Uygur Autonomous Region of China. The isolate had white aerial mycelium and brown substrate mycelium on Gause's synthetic agar. Growth occurred at 1...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2020-07, Vol.70 (7), p.4358-4363 |
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| creator | Lei, Yan-Juan Xia, Zhan-Feng Luo, Xiao-Xia Zhang, Li-Li |
| description | A novel Gram-positive, aerobic and motile endophytic actinomycete, designated TRM 65233
, was isolated from the root of
L. collected from Xinjiang Uygur Autonomous Region of China. The isolate had white aerial mycelium and brown substrate mycelium on Gause's synthetic agar. Growth occurred at 10-40 °C, pH 6-9 with NaCl concentration of 0-6 % (w/v). Strain TRM 65233
contained
-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell hydrolysates included glucose and galactose as the major whole-cell sugars. The menaquinones were MK-9 (H
) and MK-7. The major cellular fatty acids were iso-C
, iso-C
and anteiso-C
. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phospholipids, phosphatidylinositol and one unidentified lipid. Strain TRM 65233
showed the highest 16S rRNA gene sequence similarity to
BTCC B-558
(98.13 %),
IFO 16518
(98.06 %),
EG49
(97.99 %),
ID03-0561
(97.97 %),
PAL84
(97.95 %) and
44EHW
(97.06 %). The isolate was distinguished from these phylogenetically related strains by digital DNA-DNA hybridization and average nucleotide identity analyses and by a range of physiological and biochemical characteristics. The G+C content of the genomic DNA was 72.6 mol%. On the basis of polyphasic taxonomic data, strain TRM 65233
represents a novel species of the genus
, for which the name
sp. nov. is proposed. The type strain is TRM 65233
(KCTC 49342=CCTCC AA 2019050). |
| doi_str_mv | 10.1099/ijsem.0.004299 |
| format | Article |
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, was isolated from the root of
L. collected from Xinjiang Uygur Autonomous Region of China. The isolate had white aerial mycelium and brown substrate mycelium on Gause's synthetic agar. Growth occurred at 10-40 °C, pH 6-9 with NaCl concentration of 0-6 % (w/v). Strain TRM 65233
contained
-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell hydrolysates included glucose and galactose as the major whole-cell sugars. The menaquinones were MK-9 (H
) and MK-7. The major cellular fatty acids were iso-C
, iso-C
and anteiso-C
. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phospholipids, phosphatidylinositol and one unidentified lipid. Strain TRM 65233
showed the highest 16S rRNA gene sequence similarity to
BTCC B-558
(98.13 %),
IFO 16518
(98.06 %),
EG49
(97.99 %),
ID03-0561
(97.97 %),
PAL84
(97.95 %) and
44EHW
(97.06 %). The isolate was distinguished from these phylogenetically related strains by digital DNA-DNA hybridization and average nucleotide identity analyses and by a range of physiological and biochemical characteristics. The G+C content of the genomic DNA was 72.6 mol%. On the basis of polyphasic taxonomic data, strain TRM 65233
represents a novel species of the genus
, for which the name
sp. nov. is proposed. The type strain is TRM 65233
(KCTC 49342=CCTCC AA 2019050).</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijsem.0.004299</identifier><identifier>PMID: 32618556</identifier><language>eng</language><publisher>England</publisher><subject>Actinobacteria - classification ; Actinobacteria - isolation & purification ; Bacterial Typing Techniques ; Base Composition ; China ; Diaminopimelic Acid - chemistry ; DNA, Bacterial - genetics ; Fatty Acids - chemistry ; Peganum - microbiology ; Phospholipids - chemistry ; Phylogeny ; Plant Roots - microbiology ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Vitamin K 2 - analogs & derivatives ; Vitamin K 2 - chemistry</subject><ispartof>International journal of systematic and evolutionary microbiology, 2020-07, Vol.70 (7), p.4358-4363</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c335t-6d6bf1ef47eb3aaad0ff94924e39f7f0bac81a9b0b70b8f71898077fc4833c643</citedby><cites>FETCH-LOGICAL-c335t-6d6bf1ef47eb3aaad0ff94924e39f7f0bac81a9b0b70b8f71898077fc4833c643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32618556$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lei, Yan-Juan</creatorcontrib><creatorcontrib>Xia, Zhan-Feng</creatorcontrib><creatorcontrib>Luo, Xiao-Xia</creatorcontrib><creatorcontrib>Zhang, Li-Li</creatorcontrib><title>Actinokineospora pegani sp. nov., an endophytic actinomycete isolated from the surface-sterilized root of Peganum harmala L</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>A novel Gram-positive, aerobic and motile endophytic actinomycete, designated TRM 65233
, was isolated from the root of
L. collected from Xinjiang Uygur Autonomous Region of China. The isolate had white aerial mycelium and brown substrate mycelium on Gause's synthetic agar. Growth occurred at 10-40 °C, pH 6-9 with NaCl concentration of 0-6 % (w/v). Strain TRM 65233
contained
-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell hydrolysates included glucose and galactose as the major whole-cell sugars. The menaquinones were MK-9 (H
) and MK-7. The major cellular fatty acids were iso-C
, iso-C
and anteiso-C
. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phospholipids, phosphatidylinositol and one unidentified lipid. Strain TRM 65233
showed the highest 16S rRNA gene sequence similarity to
BTCC B-558
(98.13 %),
IFO 16518
(98.06 %),
EG49
(97.99 %),
ID03-0561
(97.97 %),
PAL84
(97.95 %) and
44EHW
(97.06 %). The isolate was distinguished from these phylogenetically related strains by digital DNA-DNA hybridization and average nucleotide identity analyses and by a range of physiological and biochemical characteristics. The G+C content of the genomic DNA was 72.6 mol%. On the basis of polyphasic taxonomic data, strain TRM 65233
represents a novel species of the genus
, for which the name
sp. nov. is proposed. The type strain is TRM 65233
(KCTC 49342=CCTCC AA 2019050).</description><subject>Actinobacteria - classification</subject><subject>Actinobacteria - isolation & purification</subject><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>China</subject><subject>Diaminopimelic Acid - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>Fatty Acids - chemistry</subject><subject>Peganum - microbiology</subject><subject>Phospholipids - chemistry</subject><subject>Phylogeny</subject><subject>Plant Roots - microbiology</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Vitamin K 2 - analogs & derivatives</subject><subject>Vitamin K 2 - chemistry</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtv1TAQRi0Eoi-2LJGXLEg6jh0nXlZVeUhXggVdRxNnzHVJ4mA7lS78-aa9pasZzZz5pDmMvRdQCjDm0t8lmkooAVRlzCt2KpTWRQ1SvX7pK33CzlK6A9gGAG_Ziay0aOtan7J_Vzb7Ofz2M4W0hIh8oV84e56Wks_hvvzEceY0D2HZH7K3HJ_46WApE_cpjJhp4C6Giec98bRGh5aKlCn60f_ddjGEzIPjPx6D14nvMU44It9dsDcOx0Tvnus5u_188_P6a7H7_uXb9dWusFLWudCD7p0gpxrqJSIO4JxRplIkjWsc9GhbgaaHvoG-dY1oTQtN46xqpbRayXP28Zi7xPBnpZS7ySdL44jb02vqKlWBkHUFekPLI2pjSCmS65boJ4yHTkD3KLx7Et5BdxS-HXx4zl77iYYX_L9h-QAlTH8w</recordid><startdate>202007</startdate><enddate>202007</enddate><creator>Lei, Yan-Juan</creator><creator>Xia, Zhan-Feng</creator><creator>Luo, Xiao-Xia</creator><creator>Zhang, Li-Li</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202007</creationdate><title>Actinokineospora pegani sp. nov., an endophytic actinomycete isolated from the surface-sterilized root of Peganum harmala L</title><author>Lei, Yan-Juan ; Xia, Zhan-Feng ; Luo, Xiao-Xia ; Zhang, Li-Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c335t-6d6bf1ef47eb3aaad0ff94924e39f7f0bac81a9b0b70b8f71898077fc4833c643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Actinobacteria - classification</topic><topic>Actinobacteria - isolation & purification</topic><topic>Bacterial Typing Techniques</topic><topic>Base Composition</topic><topic>China</topic><topic>Diaminopimelic Acid - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>Fatty Acids - chemistry</topic><topic>Peganum - microbiology</topic><topic>Phospholipids - chemistry</topic><topic>Phylogeny</topic><topic>Plant Roots - microbiology</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Vitamin K 2 - analogs & derivatives</topic><topic>Vitamin K 2 - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lei, Yan-Juan</creatorcontrib><creatorcontrib>Xia, Zhan-Feng</creatorcontrib><creatorcontrib>Luo, Xiao-Xia</creatorcontrib><creatorcontrib>Zhang, Li-Li</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lei, Yan-Juan</au><au>Xia, Zhan-Feng</au><au>Luo, Xiao-Xia</au><au>Zhang, Li-Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Actinokineospora pegani sp. nov., an endophytic actinomycete isolated from the surface-sterilized root of Peganum harmala L</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2020-07</date><risdate>2020</risdate><volume>70</volume><issue>7</issue><spage>4358</spage><epage>4363</epage><pages>4358-4363</pages><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>A novel Gram-positive, aerobic and motile endophytic actinomycete, designated TRM 65233
, was isolated from the root of
L. collected from Xinjiang Uygur Autonomous Region of China. The isolate had white aerial mycelium and brown substrate mycelium on Gause's synthetic agar. Growth occurred at 10-40 °C, pH 6-9 with NaCl concentration of 0-6 % (w/v). Strain TRM 65233
contained
-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell hydrolysates included glucose and galactose as the major whole-cell sugars. The menaquinones were MK-9 (H
) and MK-7. The major cellular fatty acids were iso-C
, iso-C
and anteiso-C
. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phospholipids, phosphatidylinositol and one unidentified lipid. Strain TRM 65233
showed the highest 16S rRNA gene sequence similarity to
BTCC B-558
(98.13 %),
IFO 16518
(98.06 %),
EG49
(97.99 %),
ID03-0561
(97.97 %),
PAL84
(97.95 %) and
44EHW
(97.06 %). The isolate was distinguished from these phylogenetically related strains by digital DNA-DNA hybridization and average nucleotide identity analyses and by a range of physiological and biochemical characteristics. The G+C content of the genomic DNA was 72.6 mol%. On the basis of polyphasic taxonomic data, strain TRM 65233
represents a novel species of the genus
, for which the name
sp. nov. is proposed. The type strain is TRM 65233
(KCTC 49342=CCTCC AA 2019050).</abstract><cop>England</cop><pmid>32618556</pmid><doi>10.1099/ijsem.0.004299</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Actinobacteria - classification Actinobacteria - isolation & purification Bacterial Typing Techniques Base Composition China Diaminopimelic Acid - chemistry DNA, Bacterial - genetics Fatty Acids - chemistry Peganum - microbiology Phospholipids - chemistry Phylogeny Plant Roots - microbiology RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Vitamin K 2 - analogs & derivatives Vitamin K 2 - chemistry |
| title | Actinokineospora pegani sp. nov., an endophytic actinomycete isolated from the surface-sterilized root of Peganum harmala L |
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