Global microRNA profiling identified miR‐10b‐5p as a regulator of neurofibromatosis 1 (NF1)‐glioma migration
Aims Neurofibromatosis 1 (NF1) is an autosomal‐dominant cancer predisposition syndrome caused by loss of function alterations involving the NF1 locus on chromosome 17. The most common brain tumours encountered in affected patients are low‐grade gliomas (pilocytic astrocytomas), although high‐grade g...
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| Veröffentlicht in: | Neuropathology and applied neurobiology 2021-02, Vol.47 (1), p.96-107 |
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| creator | Nix, J. S. Yuan, M. Imada, E. L. Ames, H. Marchionni, L. Gutmann, D. H. Rodriguez, F. J. |
| description | Aims
Neurofibromatosis 1 (NF1) is an autosomal‐dominant cancer predisposition syndrome caused by loss of function alterations involving the NF1 locus on chromosome 17. The most common brain tumours encountered in affected patients are low‐grade gliomas (pilocytic astrocytomas), although high‐grade gliomas are also observed at increased frequency. While bi‐allelic NF1 loss characterizes these tumours, previous studies have suggested noncoding RNA molecules (microRNA, miR) may have important roles in dictating glioma biology.
Methods
To explore the contributions of miRs in NF1‐associated gliomas, we analysed five high‐grade gliomas (NF1‐HGG) and five PAs (NF1‐PA) using global microRNA profiling with NanoString‐based microarrays followed by functional experiments with glioma cell lines.
Results
miR‐10b‐5p, miR‐135b‐5p, miR‐196a‐5p, miR‐196b‐5p, miR‐1247‐5p and miR‐320a (adjusted P 3‐fold in NF1‐HGG relative to NF1‐PA tumours. In addition, miR‐378b and miR‐1305 were decreased 6.8‐ and 6‐fold, respectively, whereas miR‐451a was increased 2.7‐fold (adjusted P |
| doi_str_mv | 10.1111/nan.12641 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2419411274</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2482317366</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3531-f1f0c5b98927a0b2e37b050d948bbbab344ed630fe04591c5aa1367ebd6c8ec43</originalsourceid><addsrcrecordid>eNp1kc1KxDAQx4Moun4cfAEJeNFDdzP5antcxC-QFUTPJWnTJZI2a7JF9uYj-Iw-iVlXPQjOIQOT3_yZmT9Cx0DGkGLSq34MVHLYQiNgUmS0LMk2GhFGRAYFl3toP8ZnQojIZbmL9hiVhAlBRyhcO6-Vw52tg3-YTfEi-NY628-xbUy_tK01Tfp9-Hh7B6LTKxZYRaxwMPPBqaUP2Le4N8O6TwffpVK0EQM-m13BeWqYO5uqSWMe1NL6_hDttMpFc_SdD9DT1eXjxU12d399ezG9y2omGGQttKQWuixKmiuiqWG5JoI0JS-01kozzk0jGWkN4aKEWiiVds-NbmRdmJqzA3S20U0rvQwmLqvOxto4p3rjh1hRDiUHoPkaPf2DPvsh9Gm6RBWUQc6kTNT5hkqnijGYtloE26mwqoBUayOqZET1ZURiT74VB92Z5pf8uXwCJhvg1Tqz-l-pmk1nG8lPeOOTvA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2482317366</pqid></control><display><type>article</type><title>Global microRNA profiling identified miR‐10b‐5p as a regulator of neurofibromatosis 1 (NF1)‐glioma migration</title><source>Wiley Journals</source><creator>Nix, J. S. ; Yuan, M. ; Imada, E. L. ; Ames, H. ; Marchionni, L. ; Gutmann, D. H. ; Rodriguez, F. J.</creator><creatorcontrib>Nix, J. S. ; Yuan, M. ; Imada, E. L. ; Ames, H. ; Marchionni, L. ; Gutmann, D. H. ; Rodriguez, F. J.</creatorcontrib><description>Aims
Neurofibromatosis 1 (NF1) is an autosomal‐dominant cancer predisposition syndrome caused by loss of function alterations involving the NF1 locus on chromosome 17. The most common brain tumours encountered in affected patients are low‐grade gliomas (pilocytic astrocytomas), although high‐grade gliomas are also observed at increased frequency. While bi‐allelic NF1 loss characterizes these tumours, previous studies have suggested noncoding RNA molecules (microRNA, miR) may have important roles in dictating glioma biology.
Methods
To explore the contributions of miRs in NF1‐associated gliomas, we analysed five high‐grade gliomas (NF1‐HGG) and five PAs (NF1‐PA) using global microRNA profiling with NanoString‐based microarrays followed by functional experiments with glioma cell lines.
Results
miR‐10b‐5p, miR‐135b‐5p, miR‐196a‐5p, miR‐196b‐5p, miR‐1247‐5p and miR‐320a (adjusted P < 0.05) were increased> 3‐fold in NF1‐HGG relative to NF1‐PA tumours. In addition, miR‐378b and miR‐1305 were decreased 6.8‐ and 6‐fold, respectively, whereas miR‐451a was increased 2.7‐fold (adjusted P < 0.05) in NF1‐PAs compared to non‐neoplastic NF1 patient brain specimens (n = 2). As miR‐10b‐5p was the microRNA overexpressed the most in NF1‐high‐grade glioma compared to NF1‐low‐grade glioma (5.76 fold), we examined its levels in glioma cell lines. miR‐10b‐5p levels were highest in adult glioma cell lines and lowest in paediatric low‐grade glioma lines (P = 0.02). miR‐10b‐5p knockdown resulted in decreased invasion in NF1‐deficient LN229 high‐grade glioma line, whereas its overexpression in the NF1‐PA derived line (JHH‐NF1‐PA1) led to increased invasion. There was no change in cell growth (viability and proliferation).
Conclusions
These proof‐of‐concept experiments support a role for microRNA regulation in NF1‐glioma biology.</description><identifier>ISSN: 0305-1846</identifier><identifier>EISSN: 1365-2990</identifier><identifier>DOI: 10.1111/nan.12641</identifier><identifier>PMID: 32603552</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Astrocytoma ; Brain cancer ; Brain tumors ; Chromosome 17 ; Genetic disorders ; Glioma ; Glioma cells ; microRNA ; MicroRNAs ; miRNA ; miR‐10b ; Neurofibromatosis ; Neurological disorders ; NF1 ; Recklinghausen's disease ; Tumors</subject><ispartof>Neuropathology and applied neurobiology, 2021-02, Vol.47 (1), p.96-107</ispartof><rights>2020 British Neuropathological Society</rights><rights>2020 British Neuropathological Society.</rights><rights>Copyright © 2021 British Neuropathological Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3531-f1f0c5b98927a0b2e37b050d948bbbab344ed630fe04591c5aa1367ebd6c8ec43</citedby><cites>FETCH-LOGICAL-c3531-f1f0c5b98927a0b2e37b050d948bbbab344ed630fe04591c5aa1367ebd6c8ec43</cites><orcidid>0000-0001-8662-1219</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fnan.12641$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fnan.12641$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32603552$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nix, J. S.</creatorcontrib><creatorcontrib>Yuan, M.</creatorcontrib><creatorcontrib>Imada, E. L.</creatorcontrib><creatorcontrib>Ames, H.</creatorcontrib><creatorcontrib>Marchionni, L.</creatorcontrib><creatorcontrib>Gutmann, D. H.</creatorcontrib><creatorcontrib>Rodriguez, F. J.</creatorcontrib><title>Global microRNA profiling identified miR‐10b‐5p as a regulator of neurofibromatosis 1 (NF1)‐glioma migration</title><title>Neuropathology and applied neurobiology</title><addtitle>Neuropathol Appl Neurobiol</addtitle><description>Aims
Neurofibromatosis 1 (NF1) is an autosomal‐dominant cancer predisposition syndrome caused by loss of function alterations involving the NF1 locus on chromosome 17. The most common brain tumours encountered in affected patients are low‐grade gliomas (pilocytic astrocytomas), although high‐grade gliomas are also observed at increased frequency. While bi‐allelic NF1 loss characterizes these tumours, previous studies have suggested noncoding RNA molecules (microRNA, miR) may have important roles in dictating glioma biology.
Methods
To explore the contributions of miRs in NF1‐associated gliomas, we analysed five high‐grade gliomas (NF1‐HGG) and five PAs (NF1‐PA) using global microRNA profiling with NanoString‐based microarrays followed by functional experiments with glioma cell lines.
Results
miR‐10b‐5p, miR‐135b‐5p, miR‐196a‐5p, miR‐196b‐5p, miR‐1247‐5p and miR‐320a (adjusted P < 0.05) were increased> 3‐fold in NF1‐HGG relative to NF1‐PA tumours. In addition, miR‐378b and miR‐1305 were decreased 6.8‐ and 6‐fold, respectively, whereas miR‐451a was increased 2.7‐fold (adjusted P < 0.05) in NF1‐PAs compared to non‐neoplastic NF1 patient brain specimens (n = 2). As miR‐10b‐5p was the microRNA overexpressed the most in NF1‐high‐grade glioma compared to NF1‐low‐grade glioma (5.76 fold), we examined its levels in glioma cell lines. miR‐10b‐5p levels were highest in adult glioma cell lines and lowest in paediatric low‐grade glioma lines (P = 0.02). miR‐10b‐5p knockdown resulted in decreased invasion in NF1‐deficient LN229 high‐grade glioma line, whereas its overexpression in the NF1‐PA derived line (JHH‐NF1‐PA1) led to increased invasion. There was no change in cell growth (viability and proliferation).
Conclusions
These proof‐of‐concept experiments support a role for microRNA regulation in NF1‐glioma biology.</description><subject>Astrocytoma</subject><subject>Brain cancer</subject><subject>Brain tumors</subject><subject>Chromosome 17</subject><subject>Genetic disorders</subject><subject>Glioma</subject><subject>Glioma cells</subject><subject>microRNA</subject><subject>MicroRNAs</subject><subject>miRNA</subject><subject>miR‐10b</subject><subject>Neurofibromatosis</subject><subject>Neurological disorders</subject><subject>NF1</subject><subject>Recklinghausen's disease</subject><subject>Tumors</subject><issn>0305-1846</issn><issn>1365-2990</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kc1KxDAQx4Moun4cfAEJeNFDdzP5antcxC-QFUTPJWnTJZI2a7JF9uYj-Iw-iVlXPQjOIQOT3_yZmT9Cx0DGkGLSq34MVHLYQiNgUmS0LMk2GhFGRAYFl3toP8ZnQojIZbmL9hiVhAlBRyhcO6-Vw52tg3-YTfEi-NY628-xbUy_tK01Tfp9-Hh7B6LTKxZYRaxwMPPBqaUP2Le4N8O6TwffpVK0EQM-m13BeWqYO5uqSWMe1NL6_hDttMpFc_SdD9DT1eXjxU12d399ezG9y2omGGQttKQWuixKmiuiqWG5JoI0JS-01kozzk0jGWkN4aKEWiiVds-NbmRdmJqzA3S20U0rvQwmLqvOxto4p3rjh1hRDiUHoPkaPf2DPvsh9Gm6RBWUQc6kTNT5hkqnijGYtloE26mwqoBUayOqZET1ZURiT74VB92Z5pf8uXwCJhvg1Tqz-l-pmk1nG8lPeOOTvA</recordid><startdate>202102</startdate><enddate>202102</enddate><creator>Nix, J. S.</creator><creator>Yuan, M.</creator><creator>Imada, E. L.</creator><creator>Ames, H.</creator><creator>Marchionni, L.</creator><creator>Gutmann, D. H.</creator><creator>Rodriguez, F. J.</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8662-1219</orcidid></search><sort><creationdate>202102</creationdate><title>Global microRNA profiling identified miR‐10b‐5p as a regulator of neurofibromatosis 1 (NF1)‐glioma migration</title><author>Nix, J. S. ; Yuan, M. ; Imada, E. L. ; Ames, H. ; Marchionni, L. ; Gutmann, D. H. ; Rodriguez, F. J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3531-f1f0c5b98927a0b2e37b050d948bbbab344ed630fe04591c5aa1367ebd6c8ec43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Astrocytoma</topic><topic>Brain cancer</topic><topic>Brain tumors</topic><topic>Chromosome 17</topic><topic>Genetic disorders</topic><topic>Glioma</topic><topic>Glioma cells</topic><topic>microRNA</topic><topic>MicroRNAs</topic><topic>miRNA</topic><topic>miR‐10b</topic><topic>Neurofibromatosis</topic><topic>Neurological disorders</topic><topic>NF1</topic><topic>Recklinghausen's disease</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nix, J. S.</creatorcontrib><creatorcontrib>Yuan, M.</creatorcontrib><creatorcontrib>Imada, E. L.</creatorcontrib><creatorcontrib>Ames, H.</creatorcontrib><creatorcontrib>Marchionni, L.</creatorcontrib><creatorcontrib>Gutmann, D. H.</creatorcontrib><creatorcontrib>Rodriguez, F. J.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Neuropathology and applied neurobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nix, J. S.</au><au>Yuan, M.</au><au>Imada, E. L.</au><au>Ames, H.</au><au>Marchionni, L.</au><au>Gutmann, D. H.</au><au>Rodriguez, F. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Global microRNA profiling identified miR‐10b‐5p as a regulator of neurofibromatosis 1 (NF1)‐glioma migration</atitle><jtitle>Neuropathology and applied neurobiology</jtitle><addtitle>Neuropathol Appl Neurobiol</addtitle><date>2021-02</date><risdate>2021</risdate><volume>47</volume><issue>1</issue><spage>96</spage><epage>107</epage><pages>96-107</pages><issn>0305-1846</issn><eissn>1365-2990</eissn><abstract>Aims
Neurofibromatosis 1 (NF1) is an autosomal‐dominant cancer predisposition syndrome caused by loss of function alterations involving the NF1 locus on chromosome 17. The most common brain tumours encountered in affected patients are low‐grade gliomas (pilocytic astrocytomas), although high‐grade gliomas are also observed at increased frequency. While bi‐allelic NF1 loss characterizes these tumours, previous studies have suggested noncoding RNA molecules (microRNA, miR) may have important roles in dictating glioma biology.
Methods
To explore the contributions of miRs in NF1‐associated gliomas, we analysed five high‐grade gliomas (NF1‐HGG) and five PAs (NF1‐PA) using global microRNA profiling with NanoString‐based microarrays followed by functional experiments with glioma cell lines.
Results
miR‐10b‐5p, miR‐135b‐5p, miR‐196a‐5p, miR‐196b‐5p, miR‐1247‐5p and miR‐320a (adjusted P < 0.05) were increased> 3‐fold in NF1‐HGG relative to NF1‐PA tumours. In addition, miR‐378b and miR‐1305 were decreased 6.8‐ and 6‐fold, respectively, whereas miR‐451a was increased 2.7‐fold (adjusted P < 0.05) in NF1‐PAs compared to non‐neoplastic NF1 patient brain specimens (n = 2). As miR‐10b‐5p was the microRNA overexpressed the most in NF1‐high‐grade glioma compared to NF1‐low‐grade glioma (5.76 fold), we examined its levels in glioma cell lines. miR‐10b‐5p levels were highest in adult glioma cell lines and lowest in paediatric low‐grade glioma lines (P = 0.02). miR‐10b‐5p knockdown resulted in decreased invasion in NF1‐deficient LN229 high‐grade glioma line, whereas its overexpression in the NF1‐PA derived line (JHH‐NF1‐PA1) led to increased invasion. There was no change in cell growth (viability and proliferation).
Conclusions
These proof‐of‐concept experiments support a role for microRNA regulation in NF1‐glioma biology.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>32603552</pmid><doi>10.1111/nan.12641</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-8662-1219</orcidid></addata></record> |
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| subjects | Astrocytoma Brain cancer Brain tumors Chromosome 17 Genetic disorders Glioma Glioma cells microRNA MicroRNAs miRNA miR‐10b Neurofibromatosis Neurological disorders NF1 Recklinghausen's disease Tumors |
| title | Global microRNA profiling identified miR‐10b‐5p as a regulator of neurofibromatosis 1 (NF1)‐glioma migration |
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