Adaptation of rat fast‐twitch muscle to endurance activity is underpinned by changes to protein degradation as well as protein synthesis
Muscle adaptations to exercise are underpinned by alterations to the abundance of individual proteins, which may occur through a change either to the synthesis or degradation of each protein. We used deuterium oxide (2H2O) labeling and chronic low‐frequency stimulation (CLFS) in vivo to investigate...
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| Veröffentlicht in: | The FASEB journal 2020-08, Vol.34 (8), p.10398-10417 |
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| creator | Hesketh, Stuart J. Sutherland, Hazel Lisboa, Paulo J. Jarvis, Jonathan C. Burniston, Jatin G. |
| description | Muscle adaptations to exercise are underpinned by alterations to the abundance of individual proteins, which may occur through a change either to the synthesis or degradation of each protein. We used deuterium oxide (2H2O) labeling and chronic low‐frequency stimulation (CLFS) in vivo to investigate the synthesis, abundance, and degradation of individual proteins during exercise‐induced muscle adaptation. Independent groups of rats received CLFS (10 Hz, 24 h/d) and 2H2O for 0, 10, 20, or 30 days. The extensor digitorum longus (EDL) was isolated from stimulated (Stim) and contralateral non‐stimulated (Ctrl) legs. Proteomic analysis encompassed 38 myofibrillar and 46 soluble proteins and the rates of change in abundance, synthesis, and degradation were reported in absolute (ng/d) units. Overall, synthesis and degradation made equal contributions to the adaptation of the proteome, including instances where a decrease in protein‐specific degradation primarily accounted for the increase in abundance of the protein. |
| doi_str_mv | 10.1096/fj.202000668RR |
| format | Article |
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We used deuterium oxide (2H2O) labeling and chronic low‐frequency stimulation (CLFS) in vivo to investigate the synthesis, abundance, and degradation of individual proteins during exercise‐induced muscle adaptation. Independent groups of rats received CLFS (10 Hz, 24 h/d) and 2H2O for 0, 10, 20, or 30 days. The extensor digitorum longus (EDL) was isolated from stimulated (Stim) and contralateral non‐stimulated (Ctrl) legs. Proteomic analysis encompassed 38 myofibrillar and 46 soluble proteins and the rates of change in abundance, synthesis, and degradation were reported in absolute (ng/d) units. 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| subjects | Adaptation, Physiological - physiology Animals biosynthetic labeling chronic stimulation deuterium oxide Electric Stimulation - methods Hindlimb - metabolism Hindlimb - physiology Male Muscle Fibers, Fast-Twitch - metabolism Muscle Fibers, Fast-Twitch - physiology Muscle, Skeletal - metabolism Muscle, Skeletal - physiology Physical Conditioning, Animal - physiology Protein Biosynthesis - physiology protein degradation protein synthesis Proteolysis Proteome - metabolism Proteomics - methods Rats Rats, Wistar |
| title | Adaptation of rat fast‐twitch muscle to endurance activity is underpinned by changes to protein degradation as well as protein synthesis |
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