DC-targeted gold nanoparticles as an efficient and biocompatible carrier for modulating allergic responses in sublingual immunotherapy
•SLIT is an efficient and non-invasive alternative for SCIT.•AuNPs are biocompatible carriers for diagnostic and therapeutic approaches.•DC-specific aptamers could be applied for targeted drug delivery. Sublingual immunotherapy (SLIT) was introduced to deliver allergens in an effective and non-invas...
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| Veröffentlicht in: | International immunopharmacology 2020-09, Vol.86, p.106690-106690, Article 106690 |
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| container_title | International immunopharmacology |
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| creator | Sadeghi, Mahvash Koushki, Khadijeh Mashayekhi, Kazem Ayati, Seyed Hasan Keshavarz Shahbaz, Sanaz Moghadam, Maliheh Sankian, Mojtaba |
| description | •SLIT is an efficient and non-invasive alternative for SCIT.•AuNPs are biocompatible carriers for diagnostic and therapeutic approaches.•DC-specific aptamers could be applied for targeted drug delivery.
Sublingual immunotherapy (SLIT) was introduced to deliver allergens in an effective and non-invasive route, which can be considered as an alternative for allergen-specific subcutaneous immunotherapy (SCIT). On the other hand, the use of gold nanoparticles (AuNPs) in allergen delivery has beneficial effects on sublingual immunotherapy. In addition, the molecular targeting agents like aptamers (Apt), have been widely applied for targeted drug delivery. Therefore, the current study aimed to evaluate the effects of dendritic cells (DCs)-specific Aptamer-modified AuNPs coated with ovalbumin (OVA) on the improvement of the SLIT outcome in the mouse model of allergy.
AuNPs with approximately 15 nm diameter were prepared by citrate reduction of HAuCl4. Afterward, Apt-modified AuNP complex was prepared and OVA was then loaded onto this complex. Following sensitization of Balb/c mice to OVA, SLIT was performed with Apt-AuNPs containing 5 µg OVA twice a week for a 2-month period. Allergen-specific IgE in serum, as well as cytokines secretion of spleen cells, were analyzed using ELISA. Also, nasopharyngeal lavage Fluid (NALF) was collected for total and eosinophil counts. Moreover, the lungs were removed for histopathological examination.
SLIT with Apt-modified AuNPs complex containing 5 μg OVA, decreased the IgE levels compared to the other groups. Also, IL-4 production has significantly decreased in spleen cells, while TGF-β and IFN-γ have significantly increased. The assessment of NALF in the group treated by this complex showed a decrease in total cell as well as in eosinophil count. Also, the examination of lung tissues revealed that, in the group treated by this complex, inflammation and perivascular infiltration were lesser than the other groups, which were observed in only one vessel of tissue.
It was shown that, Sublingual immunotherapy with DC specific Apt-modified AuNPs containing 5 μg OVA can improve the Th1 and Treg immunomodulatory responses. |
| doi_str_mv | 10.1016/j.intimp.2020.106690 |
| format | Article |
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Sublingual immunotherapy (SLIT) was introduced to deliver allergens in an effective and non-invasive route, which can be considered as an alternative for allergen-specific subcutaneous immunotherapy (SCIT). On the other hand, the use of gold nanoparticles (AuNPs) in allergen delivery has beneficial effects on sublingual immunotherapy. In addition, the molecular targeting agents like aptamers (Apt), have been widely applied for targeted drug delivery. Therefore, the current study aimed to evaluate the effects of dendritic cells (DCs)-specific Aptamer-modified AuNPs coated with ovalbumin (OVA) on the improvement of the SLIT outcome in the mouse model of allergy.
AuNPs with approximately 15 nm diameter were prepared by citrate reduction of HAuCl4. Afterward, Apt-modified AuNP complex was prepared and OVA was then loaded onto this complex. Following sensitization of Balb/c mice to OVA, SLIT was performed with Apt-AuNPs containing 5 µg OVA twice a week for a 2-month period. Allergen-specific IgE in serum, as well as cytokines secretion of spleen cells, were analyzed using ELISA. Also, nasopharyngeal lavage Fluid (NALF) was collected for total and eosinophil counts. Moreover, the lungs were removed for histopathological examination.
SLIT with Apt-modified AuNPs complex containing 5 μg OVA, decreased the IgE levels compared to the other groups. Also, IL-4 production has significantly decreased in spleen cells, while TGF-β and IFN-γ have significantly increased. The assessment of NALF in the group treated by this complex showed a decrease in total cell as well as in eosinophil count. Also, the examination of lung tissues revealed that, in the group treated by this complex, inflammation and perivascular infiltration were lesser than the other groups, which were observed in only one vessel of tissue.
It was shown that, Sublingual immunotherapy with DC specific Apt-modified AuNPs containing 5 μg OVA can improve the Th1 and Treg immunomodulatory responses.</description><identifier>ISSN: 1567-5769</identifier><identifier>EISSN: 1878-1705</identifier><identifier>DOI: 10.1016/j.intimp.2020.106690</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Allergens ; Allergies ; Approximation ; Aptamers ; Biocompatibility ; Citric acid ; Cytokines ; DC-specific aptamer ; Dendritic cells ; Drug delivery ; Drug delivery systems ; Enzyme-linked immunosorbent assay ; Gold ; Gold nanoparticles (AuNPs) ; IgE ; Immunoglobulin E ; Immunomodulation ; Immunotherapy ; Interleukin 4 ; Leukocytes (eosinophilic) ; Lungs ; Lymphocytes T ; Nanoparticles ; Oral administration ; Ovalbumin ; Ovalbumin (OVA) ; Reagents ; Spleen ; Sublingual immunotherapy (SLIT) ; γ-Interferon</subject><ispartof>International immunopharmacology, 2020-09, Vol.86, p.106690-106690, Article 106690</ispartof><rights>2020 Elsevier B.V.</rights><rights>Copyright Elsevier BV Sep 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-d140e9727b15b8de75e474ba3d2fa0af7c7d5751ba4e4e5fcfbdfc90eca76d43</citedby><cites>FETCH-LOGICAL-c367t-d140e9727b15b8de75e474ba3d2fa0af7c7d5751ba4e4e5fcfbdfc90eca76d43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.intimp.2020.106690$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids></links><search><creatorcontrib>Sadeghi, Mahvash</creatorcontrib><creatorcontrib>Koushki, Khadijeh</creatorcontrib><creatorcontrib>Mashayekhi, Kazem</creatorcontrib><creatorcontrib>Ayati, Seyed Hasan</creatorcontrib><creatorcontrib>Keshavarz Shahbaz, Sanaz</creatorcontrib><creatorcontrib>Moghadam, Maliheh</creatorcontrib><creatorcontrib>Sankian, Mojtaba</creatorcontrib><title>DC-targeted gold nanoparticles as an efficient and biocompatible carrier for modulating allergic responses in sublingual immunotherapy</title><title>International immunopharmacology</title><description>•SLIT is an efficient and non-invasive alternative for SCIT.•AuNPs are biocompatible carriers for diagnostic and therapeutic approaches.•DC-specific aptamers could be applied for targeted drug delivery.
Sublingual immunotherapy (SLIT) was introduced to deliver allergens in an effective and non-invasive route, which can be considered as an alternative for allergen-specific subcutaneous immunotherapy (SCIT). On the other hand, the use of gold nanoparticles (AuNPs) in allergen delivery has beneficial effects on sublingual immunotherapy. In addition, the molecular targeting agents like aptamers (Apt), have been widely applied for targeted drug delivery. Therefore, the current study aimed to evaluate the effects of dendritic cells (DCs)-specific Aptamer-modified AuNPs coated with ovalbumin (OVA) on the improvement of the SLIT outcome in the mouse model of allergy.
AuNPs with approximately 15 nm diameter were prepared by citrate reduction of HAuCl4. Afterward, Apt-modified AuNP complex was prepared and OVA was then loaded onto this complex. Following sensitization of Balb/c mice to OVA, SLIT was performed with Apt-AuNPs containing 5 µg OVA twice a week for a 2-month period. Allergen-specific IgE in serum, as well as cytokines secretion of spleen cells, were analyzed using ELISA. Also, nasopharyngeal lavage Fluid (NALF) was collected for total and eosinophil counts. Moreover, the lungs were removed for histopathological examination.
SLIT with Apt-modified AuNPs complex containing 5 μg OVA, decreased the IgE levels compared to the other groups. Also, IL-4 production has significantly decreased in spleen cells, while TGF-β and IFN-γ have significantly increased. The assessment of NALF in the group treated by this complex showed a decrease in total cell as well as in eosinophil count. Also, the examination of lung tissues revealed that, in the group treated by this complex, inflammation and perivascular infiltration were lesser than the other groups, which were observed in only one vessel of tissue.
It was shown that, Sublingual immunotherapy with DC specific Apt-modified AuNPs containing 5 μg OVA can improve the Th1 and Treg immunomodulatory responses.</description><subject>Allergens</subject><subject>Allergies</subject><subject>Approximation</subject><subject>Aptamers</subject><subject>Biocompatibility</subject><subject>Citric acid</subject><subject>Cytokines</subject><subject>DC-specific aptamer</subject><subject>Dendritic cells</subject><subject>Drug delivery</subject><subject>Drug delivery systems</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Gold</subject><subject>Gold nanoparticles (AuNPs)</subject><subject>IgE</subject><subject>Immunoglobulin E</subject><subject>Immunomodulation</subject><subject>Immunotherapy</subject><subject>Interleukin 4</subject><subject>Leukocytes (eosinophilic)</subject><subject>Lungs</subject><subject>Lymphocytes T</subject><subject>Nanoparticles</subject><subject>Oral administration</subject><subject>Ovalbumin</subject><subject>Ovalbumin (OVA)</subject><subject>Reagents</subject><subject>Spleen</subject><subject>Sublingual immunotherapy (SLIT)</subject><subject>γ-Interferon</subject><issn>1567-5769</issn><issn>1878-1705</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9UcFq3DAQNaWFpmn-IAdBL7l4I9mStXsJhE2bFAK95C7G0mirRZZcSQ7kB_Ld1eKccggIpHl685h5r2kuGd0wyobr48aF4qZ509HuBA3Djn5qzthWblsmqfhc32KQrZDD7mvzLecjpRXn7Kx5vdu3BdIBCxpyiN6QACHOkIrTHjOBegJBa512GEotDBld1HGaobjRI9GQksNEbExkimbxFQ8HAt5jOjhNEuY5hly1XCB5GX39XcATN01LiOUvJphfvjdfLPiMF2_3efP06-fT_qF9_HP_e3_72Op-kKU1jFPcyU6OTIxbg1Igl3yE3nQWKFippRFSsBE4chRW29FYvaOoQQ6G9-fN1So7p_hvwVzU5LJG7yFgXLLqONuyngnKKvXHO-oxLinU4SpL9F1XbaaVxVeWTjHnhFbNyU2QXhSj6pSNOqo1G3XKRq3Z1LabtQ3rrs_VPZVP9mo0LqEuykT3scB_Xsadlg</recordid><startdate>202009</startdate><enddate>202009</enddate><creator>Sadeghi, Mahvash</creator><creator>Koushki, Khadijeh</creator><creator>Mashayekhi, Kazem</creator><creator>Ayati, Seyed Hasan</creator><creator>Keshavarz Shahbaz, Sanaz</creator><creator>Moghadam, Maliheh</creator><creator>Sankian, Mojtaba</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>202009</creationdate><title>DC-targeted gold nanoparticles as an efficient and biocompatible carrier for modulating allergic responses in sublingual immunotherapy</title><author>Sadeghi, Mahvash ; Koushki, Khadijeh ; Mashayekhi, Kazem ; Ayati, Seyed Hasan ; Keshavarz Shahbaz, Sanaz ; Moghadam, Maliheh ; Sankian, Mojtaba</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-d140e9727b15b8de75e474ba3d2fa0af7c7d5751ba4e4e5fcfbdfc90eca76d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Allergens</topic><topic>Allergies</topic><topic>Approximation</topic><topic>Aptamers</topic><topic>Biocompatibility</topic><topic>Citric acid</topic><topic>Cytokines</topic><topic>DC-specific aptamer</topic><topic>Dendritic cells</topic><topic>Drug delivery</topic><topic>Drug delivery systems</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Gold</topic><topic>Gold nanoparticles (AuNPs)</topic><topic>IgE</topic><topic>Immunoglobulin E</topic><topic>Immunomodulation</topic><topic>Immunotherapy</topic><topic>Interleukin 4</topic><topic>Leukocytes (eosinophilic)</topic><topic>Lungs</topic><topic>Lymphocytes T</topic><topic>Nanoparticles</topic><topic>Oral administration</topic><topic>Ovalbumin</topic><topic>Ovalbumin (OVA)</topic><topic>Reagents</topic><topic>Spleen</topic><topic>Sublingual immunotherapy (SLIT)</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sadeghi, Mahvash</creatorcontrib><creatorcontrib>Koushki, Khadijeh</creatorcontrib><creatorcontrib>Mashayekhi, Kazem</creatorcontrib><creatorcontrib>Ayati, Seyed Hasan</creatorcontrib><creatorcontrib>Keshavarz Shahbaz, Sanaz</creatorcontrib><creatorcontrib>Moghadam, Maliheh</creatorcontrib><creatorcontrib>Sankian, Mojtaba</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International immunopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sadeghi, Mahvash</au><au>Koushki, Khadijeh</au><au>Mashayekhi, Kazem</au><au>Ayati, Seyed Hasan</au><au>Keshavarz Shahbaz, Sanaz</au><au>Moghadam, Maliheh</au><au>Sankian, Mojtaba</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DC-targeted gold nanoparticles as an efficient and biocompatible carrier for modulating allergic responses in sublingual immunotherapy</atitle><jtitle>International immunopharmacology</jtitle><date>2020-09</date><risdate>2020</risdate><volume>86</volume><spage>106690</spage><epage>106690</epage><pages>106690-106690</pages><artnum>106690</artnum><issn>1567-5769</issn><eissn>1878-1705</eissn><abstract>•SLIT is an efficient and non-invasive alternative for SCIT.•AuNPs are biocompatible carriers for diagnostic and therapeutic approaches.•DC-specific aptamers could be applied for targeted drug delivery.
Sublingual immunotherapy (SLIT) was introduced to deliver allergens in an effective and non-invasive route, which can be considered as an alternative for allergen-specific subcutaneous immunotherapy (SCIT). On the other hand, the use of gold nanoparticles (AuNPs) in allergen delivery has beneficial effects on sublingual immunotherapy. In addition, the molecular targeting agents like aptamers (Apt), have been widely applied for targeted drug delivery. Therefore, the current study aimed to evaluate the effects of dendritic cells (DCs)-specific Aptamer-modified AuNPs coated with ovalbumin (OVA) on the improvement of the SLIT outcome in the mouse model of allergy.
AuNPs with approximately 15 nm diameter were prepared by citrate reduction of HAuCl4. Afterward, Apt-modified AuNP complex was prepared and OVA was then loaded onto this complex. Following sensitization of Balb/c mice to OVA, SLIT was performed with Apt-AuNPs containing 5 µg OVA twice a week for a 2-month period. Allergen-specific IgE in serum, as well as cytokines secretion of spleen cells, were analyzed using ELISA. Also, nasopharyngeal lavage Fluid (NALF) was collected for total and eosinophil counts. Moreover, the lungs were removed for histopathological examination.
SLIT with Apt-modified AuNPs complex containing 5 μg OVA, decreased the IgE levels compared to the other groups. Also, IL-4 production has significantly decreased in spleen cells, while TGF-β and IFN-γ have significantly increased. The assessment of NALF in the group treated by this complex showed a decrease in total cell as well as in eosinophil count. Also, the examination of lung tissues revealed that, in the group treated by this complex, inflammation and perivascular infiltration were lesser than the other groups, which were observed in only one vessel of tissue.
It was shown that, Sublingual immunotherapy with DC specific Apt-modified AuNPs containing 5 μg OVA can improve the Th1 and Treg immunomodulatory responses.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.intimp.2020.106690</doi><tpages>1</tpages></addata></record> |
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| subjects | Allergens Allergies Approximation Aptamers Biocompatibility Citric acid Cytokines DC-specific aptamer Dendritic cells Drug delivery Drug delivery systems Enzyme-linked immunosorbent assay Gold Gold nanoparticles (AuNPs) IgE Immunoglobulin E Immunomodulation Immunotherapy Interleukin 4 Leukocytes (eosinophilic) Lungs Lymphocytes T Nanoparticles Oral administration Ovalbumin Ovalbumin (OVA) Reagents Spleen Sublingual immunotherapy (SLIT) γ-Interferon |
| title | DC-targeted gold nanoparticles as an efficient and biocompatible carrier for modulating allergic responses in sublingual immunotherapy |
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