HIV-DNA undetectability during chronic HIV infection: frequency and predictive factors
Abstract Background HIV-DNA is a marker of HIV reservoirs. Objectives of the study were to determine prevalence of HIV-DNA
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| Veröffentlicht in: | Journal of antimicrobial chemotherapy 2020-10, Vol.75 (10), p.2994-2997 |
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| container_title | Journal of antimicrobial chemotherapy |
| container_volume | 75 |
| creator | Nozza, Silvia Galli, Laura Gianotti, Nicola Parisi, Mariarita Poli, Andrea Cinque, Paola Spagnuolo, Vincenzo Bruzzesi, Elena Mastrangelo, Andrea Castagna, Antonella |
| description | Abstract
Background
HIV-DNA is a marker of HIV reservoirs. Objectives of the study were to determine prevalence of HIV-DNA |
| doi_str_mv | 10.1093/jac/dkaa235 |
| format | Article |
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Background
HIV-DNA is a marker of HIV reservoirs. Objectives of the study were to determine prevalence of HIV-DNA < 100 copies/106 PBMCs in blood and to identify factors associated with this in a cohort of HIV‐1-infected subjects treated with ART and with undetectable viral load (VL).
Methods
This was a cross‐sectional study on chronic HIV‐1-infected people living with HIV (PLWH) followed up at the Department of Infectious Diseases of San Raffaele Scientific Institute on current ART without change for 12 months, with available pre-ART HIV-RNA and with undetectable VL for ≥12 months. HIV-DNA was amplified and quantified by real‐time PCR (ABI Prism 7900); limit of detectability was 100 copies/106 PBMCs. Logistic regression was used to identify predictive factors for HIV-DNA < 100 copies/106 PBMCs.
Results
Four hundred and sixty-eight PLWH were considered in the analyses, 119 (25%) with HIV-DNA < 100 copies/106 PBMCs. At multivariate analysis, we found that PLWH with lower zenith HIV-RNA, higher nadir CD4 and a shorter time between HIV diagnosis and ART start were more likely to have HIV-DNA < 100 copies/106 PBMCs, after adjustment for age, gender, calendar year of ART start, type of current ART regimen, percentage time spent with undetectable VL since ART start, current CD4 and CD4/CD8 ratio.
Conclusions
In our chronic PLWH on virological suppression for 4 years, the prevalence of HIV-DNA < 100 copies/106 PBMCs was found to be 25%. Lower zenith HIV-RNA, shorter time between HIV diagnosis and starting ART and higher CD4 nadir were independently associated with low HIV-DNA.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dkaa235</identifier><identifier>PMID: 32585684</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><ispartof>Journal of antimicrobial chemotherapy, 2020-10, Vol.75 (10), p.2994-2997</ispartof><rights>The Author(s) 2020. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please email: journals.permissions@oup.com. 2020</rights><rights>The Author(s) 2020. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please email: journals.permissions@oup.com.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-ddef09be8f2a59aa555c28b72e5598bd573df0404e6b3e1390cfac2bfec962943</citedby><cites>FETCH-LOGICAL-c427t-ddef09be8f2a59aa555c28b72e5598bd573df0404e6b3e1390cfac2bfec962943</cites><orcidid>0000-0002-2673-2731 ; 0000-0002-9656-7217</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32585684$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nozza, Silvia</creatorcontrib><creatorcontrib>Galli, Laura</creatorcontrib><creatorcontrib>Gianotti, Nicola</creatorcontrib><creatorcontrib>Parisi, Mariarita</creatorcontrib><creatorcontrib>Poli, Andrea</creatorcontrib><creatorcontrib>Cinque, Paola</creatorcontrib><creatorcontrib>Spagnuolo, Vincenzo</creatorcontrib><creatorcontrib>Bruzzesi, Elena</creatorcontrib><creatorcontrib>Mastrangelo, Andrea</creatorcontrib><creatorcontrib>Castagna, Antonella</creatorcontrib><title>HIV-DNA undetectability during chronic HIV infection: frequency and predictive factors</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>Abstract
Background
HIV-DNA is a marker of HIV reservoirs. Objectives of the study were to determine prevalence of HIV-DNA < 100 copies/106 PBMCs in blood and to identify factors associated with this in a cohort of HIV‐1-infected subjects treated with ART and with undetectable viral load (VL).
Methods
This was a cross‐sectional study on chronic HIV‐1-infected people living with HIV (PLWH) followed up at the Department of Infectious Diseases of San Raffaele Scientific Institute on current ART without change for 12 months, with available pre-ART HIV-RNA and with undetectable VL for ≥12 months. HIV-DNA was amplified and quantified by real‐time PCR (ABI Prism 7900); limit of detectability was 100 copies/106 PBMCs. Logistic regression was used to identify predictive factors for HIV-DNA < 100 copies/106 PBMCs.
Results
Four hundred and sixty-eight PLWH were considered in the analyses, 119 (25%) with HIV-DNA < 100 copies/106 PBMCs. At multivariate analysis, we found that PLWH with lower zenith HIV-RNA, higher nadir CD4 and a shorter time between HIV diagnosis and ART start were more likely to have HIV-DNA < 100 copies/106 PBMCs, after adjustment for age, gender, calendar year of ART start, type of current ART regimen, percentage time spent with undetectable VL since ART start, current CD4 and CD4/CD8 ratio.
Conclusions
In our chronic PLWH on virological suppression for 4 years, the prevalence of HIV-DNA < 100 copies/106 PBMCs was found to be 25%. Lower zenith HIV-RNA, shorter time between HIV diagnosis and starting ART and higher CD4 nadir were independently associated with low HIV-DNA.</description><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kEtLAzEURoMotlZX7iUrEWRsntOJu1IfLRTdaLdDJg9NnWbGZEbovzfS6tLVvXAP3_04AJxjdIORoOO1VGP9ISWh_AAMMctRRpDAh2CIKOLZhHE6ACcxrhFCOc-LYzCghBdpY0Owmi9W2d3TFPZem86oTlaudt0W6j44_wbVe2i8UzBh0HmbANf4W2iD-eyNV1sovYZtMNqly5eBVqquCfEUHFlZR3O2nyPw-nD_Mptny-fHxWy6zBQjky7T2lgkKlNYIrmQknOuSFFNiOFcFJXmE6otYoiZvKIGU4FUekCq1EPkRDA6Ale73DY0qVDsyo2LytS19KbpY0kYLjBFBcUJvd6hKjQxBmPLNriNDNsSo_JHZJlElnuRib7YB_fVxug_9tdcAi53QNO3_yZ9A6MRfP4</recordid><startdate>20201001</startdate><enddate>20201001</enddate><creator>Nozza, Silvia</creator><creator>Galli, Laura</creator><creator>Gianotti, Nicola</creator><creator>Parisi, Mariarita</creator><creator>Poli, Andrea</creator><creator>Cinque, Paola</creator><creator>Spagnuolo, Vincenzo</creator><creator>Bruzzesi, Elena</creator><creator>Mastrangelo, Andrea</creator><creator>Castagna, Antonella</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2673-2731</orcidid><orcidid>https://orcid.org/0000-0002-9656-7217</orcidid></search><sort><creationdate>20201001</creationdate><title>HIV-DNA undetectability during chronic HIV infection: frequency and predictive factors</title><author>Nozza, Silvia ; Galli, Laura ; Gianotti, Nicola ; Parisi, Mariarita ; Poli, Andrea ; Cinque, Paola ; Spagnuolo, Vincenzo ; Bruzzesi, Elena ; Mastrangelo, Andrea ; Castagna, Antonella</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-ddef09be8f2a59aa555c28b72e5598bd573df0404e6b3e1390cfac2bfec962943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nozza, Silvia</creatorcontrib><creatorcontrib>Galli, Laura</creatorcontrib><creatorcontrib>Gianotti, Nicola</creatorcontrib><creatorcontrib>Parisi, Mariarita</creatorcontrib><creatorcontrib>Poli, Andrea</creatorcontrib><creatorcontrib>Cinque, Paola</creatorcontrib><creatorcontrib>Spagnuolo, Vincenzo</creatorcontrib><creatorcontrib>Bruzzesi, Elena</creatorcontrib><creatorcontrib>Mastrangelo, Andrea</creatorcontrib><creatorcontrib>Castagna, Antonella</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nozza, Silvia</au><au>Galli, Laura</au><au>Gianotti, Nicola</au><au>Parisi, Mariarita</au><au>Poli, Andrea</au><au>Cinque, Paola</au><au>Spagnuolo, Vincenzo</au><au>Bruzzesi, Elena</au><au>Mastrangelo, Andrea</au><au>Castagna, Antonella</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HIV-DNA undetectability during chronic HIV infection: frequency and predictive factors</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>2020-10-01</date><risdate>2020</risdate><volume>75</volume><issue>10</issue><spage>2994</spage><epage>2997</epage><pages>2994-2997</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><abstract>Abstract
Background
HIV-DNA is a marker of HIV reservoirs. Objectives of the study were to determine prevalence of HIV-DNA < 100 copies/106 PBMCs in blood and to identify factors associated with this in a cohort of HIV‐1-infected subjects treated with ART and with undetectable viral load (VL).
Methods
This was a cross‐sectional study on chronic HIV‐1-infected people living with HIV (PLWH) followed up at the Department of Infectious Diseases of San Raffaele Scientific Institute on current ART without change for 12 months, with available pre-ART HIV-RNA and with undetectable VL for ≥12 months. HIV-DNA was amplified and quantified by real‐time PCR (ABI Prism 7900); limit of detectability was 100 copies/106 PBMCs. Logistic regression was used to identify predictive factors for HIV-DNA < 100 copies/106 PBMCs.
Results
Four hundred and sixty-eight PLWH were considered in the analyses, 119 (25%) with HIV-DNA < 100 copies/106 PBMCs. At multivariate analysis, we found that PLWH with lower zenith HIV-RNA, higher nadir CD4 and a shorter time between HIV diagnosis and ART start were more likely to have HIV-DNA < 100 copies/106 PBMCs, after adjustment for age, gender, calendar year of ART start, type of current ART regimen, percentage time spent with undetectable VL since ART start, current CD4 and CD4/CD8 ratio.
Conclusions
In our chronic PLWH on virological suppression for 4 years, the prevalence of HIV-DNA < 100 copies/106 PBMCs was found to be 25%. Lower zenith HIV-RNA, shorter time between HIV diagnosis and starting ART and higher CD4 nadir were independently associated with low HIV-DNA.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>32585684</pmid><doi>10.1093/jac/dkaa235</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0002-2673-2731</orcidid><orcidid>https://orcid.org/0000-0002-9656-7217</orcidid><oa>free_for_read</oa></addata></record> |
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| title | HIV-DNA undetectability during chronic HIV infection: frequency and predictive factors |
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