Novel pit and fissure sealant containing nano-CaF2 and dimethylaminohexadecyl methacrylate with double benefits of fluoride release and antibacterial function
Pit and fissure sealants with antibacterial and remineralization properties have broad application prospects in caries prevention. The objectives of this study were to: (1) develop a novel pit and fissure sealant containing CaF2 nanoparticles (nCaF2) and dimethylaminohexadecyl methacrylate (DMAHDM);...
Gespeichert in:
| Veröffentlicht in: | Dental materials 2020-09, Vol.36 (9), p.1241-1253 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1253 |
|---|---|
| container_issue | 9 |
| container_start_page | 1241 |
| container_title | Dental materials |
| container_volume | 36 |
| creator | Fei, Xiuzhi Li, Yuncong Weir, Michael D. Baras, Bashayer H. Wang, Haohao Wang, Suping Sun, Jirun Melo, Mary A.S. Ruan, Jianping Xu, Hockin H.K. |
| description | Pit and fissure sealants with antibacterial and remineralization properties have broad application prospects in caries prevention. The objectives of this study were to: (1) develop a novel pit and fissure sealant containing CaF2 nanoparticles (nCaF2) and dimethylaminohexadecyl methacrylate (DMAHDM); and (2) investigate the effects of nCaF2 and DMAHDM on biofilm response and fluoride (F) ion release for the first time.
Helioseal F was used as a control. Bioactive sealants were formulated with DMAHDM and nCaF2. Flow properties, enamel shear bond strength, hardness and F ion releases were measured. Streptococcus mutans (S. mutans) biofilms were grown on sealants. Biofilm metabolic activity, lactic acid production, colony-forming units (CFU), and pH of biofilm culture medium were measured.
Adding 5% DMAHDM and 20% nCaF2 did not reduce the paste flow and enamel bond strength, compared to control (p < 0.05). Hardness of sealants with 20% nCaF2 and DMAHDM was higher than control (p < 0.05). The F ion release from 20% nCaF2 was much higher than that of commercial control (p < 0.05). The sealant with DMAHDM reduced the S. mutans biofilm CFU by 4 logs. The pH in biofilm medium of the new bioactive sealant was much higher (pH 6.8) than that of commercial sealant (pH 4.66) (p < 0.05).
The new bioactive pit and fissure sealant with nCaF2 and DMAHDM achieved high fluoride release and strong antibacterial performance. This novel fluoride-releasing and antibacterial sealant is promising to inhibit caries and promote the remineralizaton of enamel and dentin. |
| doi_str_mv | 10.1016/j.dental.2020.05.010 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2416261391</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0109564120301639</els_id><sourcerecordid>2447305268</sourcerecordid><originalsourceid>FETCH-LOGICAL-c367t-c8914f36f810e1aa96299c8d64b80e883cf94b824dd1d5c0b2bee739177e2adb3</originalsourceid><addsrcrecordid>eNp9kc2OFCEUhYnRxHZm3sAFiRs3VQL1y8bEdBw1mehmZk0ouNi3Q0ML1Gi_jM8qPe3KhStuTr5zgHsIec1Zyxkf3-1bC6Fo3womWMuGlnH2jGz4PMmGMTk9J5uqyGYYe_6SvMp5zxjrheQb8vtrfARPj1ioDpY6zHlNQDNor0OhJtZcDBi-06BDbLb6VjyBFg9QdievDxjiDn5pC-bk6VnUJlW9AP2JZUdtXBcPdIEADkum0VHn15jQAk3gQWd4Cqy34aJNgYTaU7cGUzCGa_LCaZ_h5u95RR5uP95vPzd33z592X64a0w3TqUxs-S960Y3cwZcazkKKc1sx36ZGcxzZ5yso-it5XYwbBELwNRJPk0gtF26K_L2kntM8ccKuagDZgO-LgHimpXo-ShGXh0VffMPuo9rCvV1leqnjg1inCvVXyiTYs4JnDomPOh0Upypc2lqry6lqXNpig2qVlRt7y82qJ99REgqG4RgwGICU5SN-P-AP562pXE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2447305268</pqid></control><display><type>article</type><title>Novel pit and fissure sealant containing nano-CaF2 and dimethylaminohexadecyl methacrylate with double benefits of fluoride release and antibacterial function</title><source>Elsevier ScienceDirect Journals</source><creator>Fei, Xiuzhi ; Li, Yuncong ; Weir, Michael D. ; Baras, Bashayer H. ; Wang, Haohao ; Wang, Suping ; Sun, Jirun ; Melo, Mary A.S. ; Ruan, Jianping ; Xu, Hockin H.K.</creator><creatorcontrib>Fei, Xiuzhi ; Li, Yuncong ; Weir, Michael D. ; Baras, Bashayer H. ; Wang, Haohao ; Wang, Suping ; Sun, Jirun ; Melo, Mary A.S. ; Ruan, Jianping ; Xu, Hockin H.K.</creatorcontrib><description>Pit and fissure sealants with antibacterial and remineralization properties have broad application prospects in caries prevention. The objectives of this study were to: (1) develop a novel pit and fissure sealant containing CaF2 nanoparticles (nCaF2) and dimethylaminohexadecyl methacrylate (DMAHDM); and (2) investigate the effects of nCaF2 and DMAHDM on biofilm response and fluoride (F) ion release for the first time.
Helioseal F was used as a control. Bioactive sealants were formulated with DMAHDM and nCaF2. Flow properties, enamel shear bond strength, hardness and F ion releases were measured. Streptococcus mutans (S. mutans) biofilms were grown on sealants. Biofilm metabolic activity, lactic acid production, colony-forming units (CFU), and pH of biofilm culture medium were measured.
Adding 5% DMAHDM and 20% nCaF2 did not reduce the paste flow and enamel bond strength, compared to control (p < 0.05). Hardness of sealants with 20% nCaF2 and DMAHDM was higher than control (p < 0.05). The F ion release from 20% nCaF2 was much higher than that of commercial control (p < 0.05). The sealant with DMAHDM reduced the S. mutans biofilm CFU by 4 logs. The pH in biofilm medium of the new bioactive sealant was much higher (pH 6.8) than that of commercial sealant (pH 4.66) (p < 0.05).
The new bioactive pit and fissure sealant with nCaF2 and DMAHDM achieved high fluoride release and strong antibacterial performance. This novel fluoride-releasing and antibacterial sealant is promising to inhibit caries and promote the remineralizaton of enamel and dentin.</description><identifier>ISSN: 0109-5641</identifier><identifier>EISSN: 1879-0097</identifier><identifier>DOI: 10.1016/j.dental.2020.05.010</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Acid production ; Antibacterial activity ; Antimicrobial agents ; Biofilms ; Biological activity ; Bonding strength ; Calcium fluoride ; Calcium fluoride nanoparticles ; Caries inhibition ; Dental caries ; Dental enamel ; Dentin ; Dentistry ; Fluorides ; Hardness ; Lactic acid ; Nanoparticles ; pH effects ; Pit and fissure sealant ; Quaternary ammonium ; Remineralization ; Sealants ; Sealing compounds ; Streptococcus infections ; Streptococcus mutans</subject><ispartof>Dental materials, 2020-09, Vol.36 (9), p.1241-1253</ispartof><rights>2020 The Academy of Dental Materials</rights><rights>Copyright Elsevier BV Sep 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-c8914f36f810e1aa96299c8d64b80e883cf94b824dd1d5c0b2bee739177e2adb3</citedby><cites>FETCH-LOGICAL-c367t-c8914f36f810e1aa96299c8d64b80e883cf94b824dd1d5c0b2bee739177e2adb3</cites><orcidid>0000-0002-7961-3787 ; 0000-0002-0007-2966</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0109564120301639$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids></links><search><creatorcontrib>Fei, Xiuzhi</creatorcontrib><creatorcontrib>Li, Yuncong</creatorcontrib><creatorcontrib>Weir, Michael D.</creatorcontrib><creatorcontrib>Baras, Bashayer H.</creatorcontrib><creatorcontrib>Wang, Haohao</creatorcontrib><creatorcontrib>Wang, Suping</creatorcontrib><creatorcontrib>Sun, Jirun</creatorcontrib><creatorcontrib>Melo, Mary A.S.</creatorcontrib><creatorcontrib>Ruan, Jianping</creatorcontrib><creatorcontrib>Xu, Hockin H.K.</creatorcontrib><title>Novel pit and fissure sealant containing nano-CaF2 and dimethylaminohexadecyl methacrylate with double benefits of fluoride release and antibacterial function</title><title>Dental materials</title><description>Pit and fissure sealants with antibacterial and remineralization properties have broad application prospects in caries prevention. The objectives of this study were to: (1) develop a novel pit and fissure sealant containing CaF2 nanoparticles (nCaF2) and dimethylaminohexadecyl methacrylate (DMAHDM); and (2) investigate the effects of nCaF2 and DMAHDM on biofilm response and fluoride (F) ion release for the first time.
Helioseal F was used as a control. Bioactive sealants were formulated with DMAHDM and nCaF2. Flow properties, enamel shear bond strength, hardness and F ion releases were measured. Streptococcus mutans (S. mutans) biofilms were grown on sealants. Biofilm metabolic activity, lactic acid production, colony-forming units (CFU), and pH of biofilm culture medium were measured.
Adding 5% DMAHDM and 20% nCaF2 did not reduce the paste flow and enamel bond strength, compared to control (p < 0.05). Hardness of sealants with 20% nCaF2 and DMAHDM was higher than control (p < 0.05). The F ion release from 20% nCaF2 was much higher than that of commercial control (p < 0.05). The sealant with DMAHDM reduced the S. mutans biofilm CFU by 4 logs. The pH in biofilm medium of the new bioactive sealant was much higher (pH 6.8) than that of commercial sealant (pH 4.66) (p < 0.05).
The new bioactive pit and fissure sealant with nCaF2 and DMAHDM achieved high fluoride release and strong antibacterial performance. This novel fluoride-releasing and antibacterial sealant is promising to inhibit caries and promote the remineralizaton of enamel and dentin.</description><subject>Acid production</subject><subject>Antibacterial activity</subject><subject>Antimicrobial agents</subject><subject>Biofilms</subject><subject>Biological activity</subject><subject>Bonding strength</subject><subject>Calcium fluoride</subject><subject>Calcium fluoride nanoparticles</subject><subject>Caries inhibition</subject><subject>Dental caries</subject><subject>Dental enamel</subject><subject>Dentin</subject><subject>Dentistry</subject><subject>Fluorides</subject><subject>Hardness</subject><subject>Lactic acid</subject><subject>Nanoparticles</subject><subject>pH effects</subject><subject>Pit and fissure sealant</subject><subject>Quaternary ammonium</subject><subject>Remineralization</subject><subject>Sealants</subject><subject>Sealing compounds</subject><subject>Streptococcus infections</subject><subject>Streptococcus mutans</subject><issn>0109-5641</issn><issn>1879-0097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kc2OFCEUhYnRxHZm3sAFiRs3VQL1y8bEdBw1mehmZk0ouNi3Q0ML1Gi_jM8qPe3KhStuTr5zgHsIec1Zyxkf3-1bC6Fo3womWMuGlnH2jGz4PMmGMTk9J5uqyGYYe_6SvMp5zxjrheQb8vtrfARPj1ioDpY6zHlNQDNor0OhJtZcDBi-06BDbLb6VjyBFg9QdievDxjiDn5pC-bk6VnUJlW9AP2JZUdtXBcPdIEADkum0VHn15jQAk3gQWd4Cqy34aJNgYTaU7cGUzCGa_LCaZ_h5u95RR5uP95vPzd33z592X64a0w3TqUxs-S960Y3cwZcazkKKc1sx36ZGcxzZ5yso-it5XYwbBELwNRJPk0gtF26K_L2kntM8ccKuagDZgO-LgHimpXo-ShGXh0VffMPuo9rCvV1leqnjg1inCvVXyiTYs4JnDomPOh0Upypc2lqry6lqXNpig2qVlRt7y82qJ99REgqG4RgwGICU5SN-P-AP562pXE</recordid><startdate>202009</startdate><enddate>202009</enddate><creator>Fei, Xiuzhi</creator><creator>Li, Yuncong</creator><creator>Weir, Michael D.</creator><creator>Baras, Bashayer H.</creator><creator>Wang, Haohao</creator><creator>Wang, Suping</creator><creator>Sun, Jirun</creator><creator>Melo, Mary A.S.</creator><creator>Ruan, Jianping</creator><creator>Xu, Hockin H.K.</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7961-3787</orcidid><orcidid>https://orcid.org/0000-0002-0007-2966</orcidid></search><sort><creationdate>202009</creationdate><title>Novel pit and fissure sealant containing nano-CaF2 and dimethylaminohexadecyl methacrylate with double benefits of fluoride release and antibacterial function</title><author>Fei, Xiuzhi ; Li, Yuncong ; Weir, Michael D. ; Baras, Bashayer H. ; Wang, Haohao ; Wang, Suping ; Sun, Jirun ; Melo, Mary A.S. ; Ruan, Jianping ; Xu, Hockin H.K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-c8914f36f810e1aa96299c8d64b80e883cf94b824dd1d5c0b2bee739177e2adb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acid production</topic><topic>Antibacterial activity</topic><topic>Antimicrobial agents</topic><topic>Biofilms</topic><topic>Biological activity</topic><topic>Bonding strength</topic><topic>Calcium fluoride</topic><topic>Calcium fluoride nanoparticles</topic><topic>Caries inhibition</topic><topic>Dental caries</topic><topic>Dental enamel</topic><topic>Dentin</topic><topic>Dentistry</topic><topic>Fluorides</topic><topic>Hardness</topic><topic>Lactic acid</topic><topic>Nanoparticles</topic><topic>pH effects</topic><topic>Pit and fissure sealant</topic><topic>Quaternary ammonium</topic><topic>Remineralization</topic><topic>Sealants</topic><topic>Sealing compounds</topic><topic>Streptococcus infections</topic><topic>Streptococcus mutans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fei, Xiuzhi</creatorcontrib><creatorcontrib>Li, Yuncong</creatorcontrib><creatorcontrib>Weir, Michael D.</creatorcontrib><creatorcontrib>Baras, Bashayer H.</creatorcontrib><creatorcontrib>Wang, Haohao</creatorcontrib><creatorcontrib>Wang, Suping</creatorcontrib><creatorcontrib>Sun, Jirun</creatorcontrib><creatorcontrib>Melo, Mary A.S.</creatorcontrib><creatorcontrib>Ruan, Jianping</creatorcontrib><creatorcontrib>Xu, Hockin H.K.</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Dental materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fei, Xiuzhi</au><au>Li, Yuncong</au><au>Weir, Michael D.</au><au>Baras, Bashayer H.</au><au>Wang, Haohao</au><au>Wang, Suping</au><au>Sun, Jirun</au><au>Melo, Mary A.S.</au><au>Ruan, Jianping</au><au>Xu, Hockin H.K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel pit and fissure sealant containing nano-CaF2 and dimethylaminohexadecyl methacrylate with double benefits of fluoride release and antibacterial function</atitle><jtitle>Dental materials</jtitle><date>2020-09</date><risdate>2020</risdate><volume>36</volume><issue>9</issue><spage>1241</spage><epage>1253</epage><pages>1241-1253</pages><issn>0109-5641</issn><eissn>1879-0097</eissn><abstract>Pit and fissure sealants with antibacterial and remineralization properties have broad application prospects in caries prevention. The objectives of this study were to: (1) develop a novel pit and fissure sealant containing CaF2 nanoparticles (nCaF2) and dimethylaminohexadecyl methacrylate (DMAHDM); and (2) investigate the effects of nCaF2 and DMAHDM on biofilm response and fluoride (F) ion release for the first time.
Helioseal F was used as a control. Bioactive sealants were formulated with DMAHDM and nCaF2. Flow properties, enamel shear bond strength, hardness and F ion releases were measured. Streptococcus mutans (S. mutans) biofilms were grown on sealants. Biofilm metabolic activity, lactic acid production, colony-forming units (CFU), and pH of biofilm culture medium were measured.
Adding 5% DMAHDM and 20% nCaF2 did not reduce the paste flow and enamel bond strength, compared to control (p < 0.05). Hardness of sealants with 20% nCaF2 and DMAHDM was higher than control (p < 0.05). The F ion release from 20% nCaF2 was much higher than that of commercial control (p < 0.05). The sealant with DMAHDM reduced the S. mutans biofilm CFU by 4 logs. The pH in biofilm medium of the new bioactive sealant was much higher (pH 6.8) than that of commercial sealant (pH 4.66) (p < 0.05).
The new bioactive pit and fissure sealant with nCaF2 and DMAHDM achieved high fluoride release and strong antibacterial performance. This novel fluoride-releasing and antibacterial sealant is promising to inhibit caries and promote the remineralizaton of enamel and dentin.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><doi>10.1016/j.dental.2020.05.010</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-7961-3787</orcidid><orcidid>https://orcid.org/0000-0002-0007-2966</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0109-5641 |
| ispartof | Dental materials, 2020-09, Vol.36 (9), p.1241-1253 |
| issn | 0109-5641 1879-0097 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2416261391 |
| source | Elsevier ScienceDirect Journals |
| subjects | Acid production Antibacterial activity Antimicrobial agents Biofilms Biological activity Bonding strength Calcium fluoride Calcium fluoride nanoparticles Caries inhibition Dental caries Dental enamel Dentin Dentistry Fluorides Hardness Lactic acid Nanoparticles pH effects Pit and fissure sealant Quaternary ammonium Remineralization Sealants Sealing compounds Streptococcus infections Streptococcus mutans |
| title | Novel pit and fissure sealant containing nano-CaF2 and dimethylaminohexadecyl methacrylate with double benefits of fluoride release and antibacterial function |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T07%3A13%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Novel%20pit%20and%20fissure%20sealant%20containing%20nano-CaF2%20and%20dimethylaminohexadecyl%20methacrylate%20with%20double%20benefits%20of%20fluoride%20release%20and%20antibacterial%20function&rft.jtitle=Dental%20materials&rft.au=Fei,%20Xiuzhi&rft.date=2020-09&rft.volume=36&rft.issue=9&rft.spage=1241&rft.epage=1253&rft.pages=1241-1253&rft.issn=0109-5641&rft.eissn=1879-0097&rft_id=info:doi/10.1016/j.dental.2020.05.010&rft_dat=%3Cproquest_cross%3E2447305268%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2447305268&rft_id=info:pmid/&rft_els_id=S0109564120301639&rfr_iscdi=true |