Recent progress in metabolic engineering of microbial formate assimilation
Formate can be efficiently produced via electrochemical or photochemical catalytic conversion of CO 2 , and it can be directly used as an organic carbon source by microorganisms. In theory, formate can be used as the sole carbon source for the microbial production of high-value-added chemicals. Cons...
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creator | Mao, Wen Yuan, Qianqian Qi, Hongge Wang, Zhiwen Ma, Hongwu Chen, Tao |
description | Formate can be efficiently produced via electrochemical or photochemical catalytic conversion of CO
2
, and it can be directly used as an organic carbon source by microorganisms. In theory, formate can be used as the sole carbon source for the microbial production of high-value-added chemicals. Consequently, the construction of efficient formate-assimilation pathways in microorganisms is essential for the utilization of cheap, renewable one-carbon compounds. This paper summarizes new methods of formate synthesis, as well as the natural formate utilization pathways of microorganisms with their advantages and disadvantages. Furthermore, it reviews recent progress in the design of utilization pathways for formate in microbial cells through metabolic engineering and synthetic biology. Besides, we also use the pathway-prediction algorithm comb-FBA to rationally design completely new one-carbon compounds utilization pathways. The pathway with the highest efficiency, named GAA, was corroborated by the in vitro experiments showing a carbon molar yield up to 88%. Finally, it discusses the main problems and challenges presently existing in the pathway design and strain improvement for microbial utilization of formate.
Key points
•
Natural and artificial design pathways of formate-assimilation was summarized.
•
Recent progresses in different hosts and approaches of using one-carbon compounds was reviewed.
•
Metabolic engineering and synthetic biology methods to improve formate utilization were discussed. |
doi_str_mv | 10.1007/s00253-020-10725-6 |
format | Article |
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2
, and it can be directly used as an organic carbon source by microorganisms. In theory, formate can be used as the sole carbon source for the microbial production of high-value-added chemicals. Consequently, the construction of efficient formate-assimilation pathways in microorganisms is essential for the utilization of cheap, renewable one-carbon compounds. This paper summarizes new methods of formate synthesis, as well as the natural formate utilization pathways of microorganisms with their advantages and disadvantages. Furthermore, it reviews recent progress in the design of utilization pathways for formate in microbial cells through metabolic engineering and synthetic biology. Besides, we also use the pathway-prediction algorithm comb-FBA to rationally design completely new one-carbon compounds utilization pathways. The pathway with the highest efficiency, named GAA, was corroborated by the in vitro experiments showing a carbon molar yield up to 88%. Finally, it discusses the main problems and challenges presently existing in the pathway design and strain improvement for microbial utilization of formate.
Key points
•
Natural and artificial design pathways of formate-assimilation was summarized.
•
Recent progresses in different hosts and approaches of using one-carbon compounds was reviewed.
•
Metabolic engineering and synthetic biology methods to improve formate utilization were discussed.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-020-10725-6</identifier><identifier>PMID: 32566995</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Algorithms ; Assimilation ; Bacteria - metabolism ; Biology ; Biomedical and Life Sciences ; Biotechnology ; Carbon ; Carbon - metabolism ; Carbon compounds ; Carbon dioxide ; Carbon Dioxide - metabolism ; Carbon sources ; Catalytic converters ; Design ; Electrochemistry ; Formates - metabolism ; Life Sciences ; Metabolic engineering ; Metabolic Engineering - methods ; Metabolic Engineering - trends ; Metabolism ; Microbial Genetics and Genomics ; Microbiology ; Microorganisms ; Mini-Review ; Organic carbon ; Photochemicals ; Synthetic Biology ; Utilization</subject><ispartof>Applied microbiology and biotechnology, 2020-08, Vol.104 (16), p.6905-6917</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>COPYRIGHT 2020 Springer</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-d813a542bf1e137b7f1bc3177ab7a28629991791d34ed1abea7a586902b4decf3</citedby><cites>FETCH-LOGICAL-c513t-d813a542bf1e137b7f1bc3177ab7a28629991791d34ed1abea7a586902b4decf3</cites><orcidid>0000-0001-9588-1821</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-020-10725-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-020-10725-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32566995$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mao, Wen</creatorcontrib><creatorcontrib>Yuan, Qianqian</creatorcontrib><creatorcontrib>Qi, Hongge</creatorcontrib><creatorcontrib>Wang, Zhiwen</creatorcontrib><creatorcontrib>Ma, Hongwu</creatorcontrib><creatorcontrib>Chen, Tao</creatorcontrib><title>Recent progress in metabolic engineering of microbial formate assimilation</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>Formate can be efficiently produced via electrochemical or photochemical catalytic conversion of CO
2
, and it can be directly used as an organic carbon source by microorganisms. In theory, formate can be used as the sole carbon source for the microbial production of high-value-added chemicals. Consequently, the construction of efficient formate-assimilation pathways in microorganisms is essential for the utilization of cheap, renewable one-carbon compounds. This paper summarizes new methods of formate synthesis, as well as the natural formate utilization pathways of microorganisms with their advantages and disadvantages. Furthermore, it reviews recent progress in the design of utilization pathways for formate in microbial cells through metabolic engineering and synthetic biology. Besides, we also use the pathway-prediction algorithm comb-FBA to rationally design completely new one-carbon compounds utilization pathways. The pathway with the highest efficiency, named GAA, was corroborated by the in vitro experiments showing a carbon molar yield up to 88%. Finally, it discusses the main problems and challenges presently existing in the pathway design and strain improvement for microbial utilization of formate.
Key points
•
Natural and artificial design pathways of formate-assimilation was summarized.
•
Recent progresses in different hosts and approaches of using one-carbon compounds was reviewed.
•
Metabolic engineering and synthetic biology methods to improve formate utilization were discussed.</description><subject>Algorithms</subject><subject>Assimilation</subject><subject>Bacteria - metabolism</subject><subject>Biology</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Carbon</subject><subject>Carbon - metabolism</subject><subject>Carbon compounds</subject><subject>Carbon dioxide</subject><subject>Carbon Dioxide - metabolism</subject><subject>Carbon sources</subject><subject>Catalytic converters</subject><subject>Design</subject><subject>Electrochemistry</subject><subject>Formates - metabolism</subject><subject>Life Sciences</subject><subject>Metabolic engineering</subject><subject>Metabolic Engineering - methods</subject><subject>Metabolic Engineering - trends</subject><subject>Metabolism</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Mini-Review</subject><subject>Organic carbon</subject><subject>Photochemicals</subject><subject>Synthetic Biology</subject><subject>Utilization</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUFrFTEUhYMo9ln9Ay5kwE1dTJubTJKZZSlWK4VC1XVIZm6GlJnkmcyA_nvz-qrlSZEsLoTvHO49h5C3QE-BUnWWKWWC15TRGqhiopbPyAYazmoqoXlONhSUqJXo2iPyKuc7SoG1Ur4kR5wJKbtObMiXW-wxLNU2xTFhzpUP1YyLsXHyfYVh9AEx-TBW0VWz71O03kyVi2k2C1YmZz_7ySw-htfkhTNTxjcP85h8v_z47eJzfX3z6eri_LruBfClHlrgRjTMOkDgyioHtueglLHKlP1Y13WgOhh4gwMYi0YZ0cqOMtsM2Dt-TE72vmXnHyvmRc8-9zhNJmBcs2YNiJYrStuCvv8HvYtrCmW7QjGhmFJSPVKjmVD74OKSTL8z1eeSl3gBKBTq9AmqvAFLLjGg8-X_QPDhQFCYBX8uo1lz1ldfbw9ZtmdLwDkndHqb_GzSLw1U78rW-7J1Eej7srUsoncP1612xuGv5E-7BeB7IG93FWJ6PP8_tr8BxJexXg</recordid><startdate>20200801</startdate><enddate>20200801</enddate><creator>Mao, Wen</creator><creator>Yuan, Qianqian</creator><creator>Qi, Hongge</creator><creator>Wang, Zhiwen</creator><creator>Ma, Hongwu</creator><creator>Chen, Tao</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9588-1821</orcidid></search><sort><creationdate>20200801</creationdate><title>Recent progress in metabolic engineering of microbial formate assimilation</title><author>Mao, Wen ; Yuan, Qianqian ; Qi, Hongge ; Wang, Zhiwen ; Ma, Hongwu ; Chen, Tao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-d813a542bf1e137b7f1bc3177ab7a28629991791d34ed1abea7a586902b4decf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Algorithms</topic><topic>Assimilation</topic><topic>Bacteria - 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Academic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mao, Wen</au><au>Yuan, Qianqian</au><au>Qi, Hongge</au><au>Wang, Zhiwen</au><au>Ma, Hongwu</au><au>Chen, Tao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recent progress in metabolic engineering of microbial formate assimilation</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2020-08-01</date><risdate>2020</risdate><volume>104</volume><issue>16</issue><spage>6905</spage><epage>6917</epage><pages>6905-6917</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>Formate can be efficiently produced via electrochemical or photochemical catalytic conversion of CO
2
, and it can be directly used as an organic carbon source by microorganisms. In theory, formate can be used as the sole carbon source for the microbial production of high-value-added chemicals. Consequently, the construction of efficient formate-assimilation pathways in microorganisms is essential for the utilization of cheap, renewable one-carbon compounds. This paper summarizes new methods of formate synthesis, as well as the natural formate utilization pathways of microorganisms with their advantages and disadvantages. Furthermore, it reviews recent progress in the design of utilization pathways for formate in microbial cells through metabolic engineering and synthetic biology. Besides, we also use the pathway-prediction algorithm comb-FBA to rationally design completely new one-carbon compounds utilization pathways. The pathway with the highest efficiency, named GAA, was corroborated by the in vitro experiments showing a carbon molar yield up to 88%. Finally, it discusses the main problems and challenges presently existing in the pathway design and strain improvement for microbial utilization of formate.
Key points
•
Natural and artificial design pathways of formate-assimilation was summarized.
•
Recent progresses in different hosts and approaches of using one-carbon compounds was reviewed.
•
Metabolic engineering and synthetic biology methods to improve formate utilization were discussed.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32566995</pmid><doi>10.1007/s00253-020-10725-6</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-9588-1821</orcidid></addata></record> |
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subjects | Algorithms Assimilation Bacteria - metabolism Biology Biomedical and Life Sciences Biotechnology Carbon Carbon - metabolism Carbon compounds Carbon dioxide Carbon Dioxide - metabolism Carbon sources Catalytic converters Design Electrochemistry Formates - metabolism Life Sciences Metabolic engineering Metabolic Engineering - methods Metabolic Engineering - trends Metabolism Microbial Genetics and Genomics Microbiology Microorganisms Mini-Review Organic carbon Photochemicals Synthetic Biology Utilization |
title | Recent progress in metabolic engineering of microbial formate assimilation |
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