Distinct effects of different adjuvants in the mouse model of allergic airway inflammation
Allergic asthma was typically considered as an inflammatory disease mediated by type 2 immunity. However, recent studies revealed that asthma is a complex disease displaying a variety of phenotypes and endotypes. We examined cellular phenotypes in the mouse model of allergic asthma sensitized with d...
Gespeichert in:
| Veröffentlicht in: | Asian Pacific journal of allergy and immunology 2022-06, Vol.40 (2), p.111-120 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 120 |
|---|---|
| container_issue | 2 |
| container_start_page | 111 |
| container_title | Asian Pacific journal of allergy and immunology |
| container_volume | 40 |
| creator | Shin, Hyun Soo Chun, Hye Rin Na, Hye Young Sohn, Moah Ryu, Seul Hye Choi, Wanho In, BaoqingHyunju Park, Ji Soo Park, Sejung Park, Chae Gyu |
| description | Allergic asthma was typically considered as an inflammatory disease mediated by type 2 immunity. However, recent studies revealed that asthma is a complex disease displaying a variety of phenotypes and endotypes.
We examined cellular phenotypes in the mouse model of allergic asthma sensitized with different adjuvants. The aim of our study was to determine immunologic cellular characteristics in mouse asthma models induced by ovalbumin (OVA) and a variety of adjuvants.
Mice were sensitized intraperitoneally with the admixture of OVA and various adjuvants such as Alhydrogel (alum), papain, lipopolysaccharide (LPS), or CpG, and subsequently challenged with OVA intranasally. The cells in bronchoalveolar lavage (BAL) fluid, lung, and mediastinal lymph node (mLN) were examined by flow cytometric analyses.
In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group. Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node. In the lung, the number of T-bet+ ILCs was highest in the papain group whereas the number of IFN-γ+ Th cells was highest in the CpG group.
Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants. |
| doi_str_mv | 10.12932/AP-301219-0729 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2415836516</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2672062163</sourcerecordid><originalsourceid>FETCH-LOGICAL-c436t-ad2bf54732d4649fe7358d5e93b15de7f1e7dd34646408cfccf9a31b9923cc733</originalsourceid><addsrcrecordid>eNpd0MtOAyEUBmBiNLapXbszk7hxM3aAAYZlU69JE7vQxLghDBelmUsFRtO3l1p1IQsgnI-Tkx-AU1hcQsQxms1XOS4ggjwvGOIHYIwQqvKK8vIQjFOB5BVjzyMwDWFdpEUhrEh5DEYYEYqTHYOXKxei61TMjLVGxZD1NtMu3b3pYib1eviQXXp2XRbfTNb2Q9jt2jQ7KZvG-FenMun8p9wmZRvZtjK6vjsBR1Y2wUx_zgl4url-XNzly4fb-8V8masS05hLjWpLSoaRLmnJrWGYVJoYjmtItGEWGqY1TjVaFpWySlkuMaw5R1gphvEEXOz7bnz_PpgQReuCMk0jO5OmFaiEpMKUQJro-T-67gffpekEogwVFCWU1GyvlO9D8MaKjXet9FsBC_GdvJivxD55sUs-_Tj76TvUrdF__jdn_AVj3H3V</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2672062163</pqid></control><display><type>article</type><title>Distinct effects of different adjuvants in the mouse model of allergic airway inflammation</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Shin, Hyun Soo ; Chun, Hye Rin ; Na, Hye Young ; Sohn, Moah ; Ryu, Seul Hye ; Choi, Wanho ; In, BaoqingHyunju ; Park, Ji Soo ; Park, Sejung ; Park, Chae Gyu</creator><creatorcontrib>Shin, Hyun Soo ; Chun, Hye Rin ; Na, Hye Young ; Sohn, Moah ; Ryu, Seul Hye ; Choi, Wanho ; In, BaoqingHyunju ; Park, Ji Soo ; Park, Sejung ; Park, Chae Gyu</creatorcontrib><description>Allergic asthma was typically considered as an inflammatory disease mediated by type 2 immunity. However, recent studies revealed that asthma is a complex disease displaying a variety of phenotypes and endotypes.
We examined cellular phenotypes in the mouse model of allergic asthma sensitized with different adjuvants. The aim of our study was to determine immunologic cellular characteristics in mouse asthma models induced by ovalbumin (OVA) and a variety of adjuvants.
Mice were sensitized intraperitoneally with the admixture of OVA and various adjuvants such as Alhydrogel (alum), papain, lipopolysaccharide (LPS), or CpG, and subsequently challenged with OVA intranasally. The cells in bronchoalveolar lavage (BAL) fluid, lung, and mediastinal lymph node (mLN) were examined by flow cytometric analyses.
In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group. Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node. In the lung, the number of T-bet+ ILCs was highest in the papain group whereas the number of IFN-γ+ Th cells was highest in the CpG group.
Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.</description><identifier>ISSN: 0125-877X</identifier><identifier>EISSN: 2228-8694</identifier><identifier>DOI: 10.12932/AP-301219-0729</identifier><identifier>PMID: 32563228</identifier><language>eng</language><publisher>Thailand: The Allergy and Immunology Society</publisher><subject>Adjuvants ; Adjuvants, Immunologic ; Animal models ; Animals ; Asthma ; Asthma - etiology ; Bronchoalveolar Lavage Fluid ; Bronchus ; Cytokines ; Disease Models, Animal ; Flow cytometry ; Genotype & phenotype ; Helper cells ; Humans ; Immunity, Innate ; Inflammation ; Inflammatory diseases ; Lavage ; Leukocytes (eosinophilic) ; Leukocytes (neutrophilic) ; Lipopolysaccharides ; Lung - pathology ; Lymph nodes ; Lymphocytes - metabolism ; Lymphocytes T ; Lymphoid cells ; Mice ; Mice, Inbred BALB C ; Ovalbumin ; Papain ; Papain - metabolism ; Phenotypes ; Respiratory tract diseases ; γ-Interferon</subject><ispartof>Asian Pacific journal of allergy and immunology, 2022-06, Vol.40 (2), p.111-120</ispartof><rights>Copyright The Allergy and Immunology Society Jun 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-ad2bf54732d4649fe7358d5e93b15de7f1e7dd34646408cfccf9a31b9923cc733</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32563228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shin, Hyun Soo</creatorcontrib><creatorcontrib>Chun, Hye Rin</creatorcontrib><creatorcontrib>Na, Hye Young</creatorcontrib><creatorcontrib>Sohn, Moah</creatorcontrib><creatorcontrib>Ryu, Seul Hye</creatorcontrib><creatorcontrib>Choi, Wanho</creatorcontrib><creatorcontrib>In, BaoqingHyunju</creatorcontrib><creatorcontrib>Park, Ji Soo</creatorcontrib><creatorcontrib>Park, Sejung</creatorcontrib><creatorcontrib>Park, Chae Gyu</creatorcontrib><title>Distinct effects of different adjuvants in the mouse model of allergic airway inflammation</title><title>Asian Pacific journal of allergy and immunology</title><addtitle>Asian Pac J Allergy Immunol</addtitle><description>Allergic asthma was typically considered as an inflammatory disease mediated by type 2 immunity. However, recent studies revealed that asthma is a complex disease displaying a variety of phenotypes and endotypes.
We examined cellular phenotypes in the mouse model of allergic asthma sensitized with different adjuvants. The aim of our study was to determine immunologic cellular characteristics in mouse asthma models induced by ovalbumin (OVA) and a variety of adjuvants.
Mice were sensitized intraperitoneally with the admixture of OVA and various adjuvants such as Alhydrogel (alum), papain, lipopolysaccharide (LPS), or CpG, and subsequently challenged with OVA intranasally. The cells in bronchoalveolar lavage (BAL) fluid, lung, and mediastinal lymph node (mLN) were examined by flow cytometric analyses.
In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group. Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node. In the lung, the number of T-bet+ ILCs was highest in the papain group whereas the number of IFN-γ+ Th cells was highest in the CpG group.
Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.</description><subject>Adjuvants</subject><subject>Adjuvants, Immunologic</subject><subject>Animal models</subject><subject>Animals</subject><subject>Asthma</subject><subject>Asthma - etiology</subject><subject>Bronchoalveolar Lavage Fluid</subject><subject>Bronchus</subject><subject>Cytokines</subject><subject>Disease Models, Animal</subject><subject>Flow cytometry</subject><subject>Genotype & phenotype</subject><subject>Helper cells</subject><subject>Humans</subject><subject>Immunity, Innate</subject><subject>Inflammation</subject><subject>Inflammatory diseases</subject><subject>Lavage</subject><subject>Leukocytes (eosinophilic)</subject><subject>Leukocytes (neutrophilic)</subject><subject>Lipopolysaccharides</subject><subject>Lung - pathology</subject><subject>Lymph nodes</subject><subject>Lymphocytes - metabolism</subject><subject>Lymphocytes T</subject><subject>Lymphoid cells</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Ovalbumin</subject><subject>Papain</subject><subject>Papain - metabolism</subject><subject>Phenotypes</subject><subject>Respiratory tract diseases</subject><subject>γ-Interferon</subject><issn>0125-877X</issn><issn>2228-8694</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpd0MtOAyEUBmBiNLapXbszk7hxM3aAAYZlU69JE7vQxLghDBelmUsFRtO3l1p1IQsgnI-Tkx-AU1hcQsQxms1XOS4ggjwvGOIHYIwQqvKK8vIQjFOB5BVjzyMwDWFdpEUhrEh5DEYYEYqTHYOXKxei61TMjLVGxZD1NtMu3b3pYib1eviQXXp2XRbfTNb2Q9jt2jQ7KZvG-FenMun8p9wmZRvZtjK6vjsBR1Y2wUx_zgl4url-XNzly4fb-8V8masS05hLjWpLSoaRLmnJrWGYVJoYjmtItGEWGqY1TjVaFpWySlkuMaw5R1gphvEEXOz7bnz_PpgQReuCMk0jO5OmFaiEpMKUQJro-T-67gffpekEogwVFCWU1GyvlO9D8MaKjXet9FsBC_GdvJivxD55sUs-_Tj76TvUrdF__jdn_AVj3H3V</recordid><startdate>20220601</startdate><enddate>20220601</enddate><creator>Shin, Hyun Soo</creator><creator>Chun, Hye Rin</creator><creator>Na, Hye Young</creator><creator>Sohn, Moah</creator><creator>Ryu, Seul Hye</creator><creator>Choi, Wanho</creator><creator>In, BaoqingHyunju</creator><creator>Park, Ji Soo</creator><creator>Park, Sejung</creator><creator>Park, Chae Gyu</creator><general>The Allergy and Immunology Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BVBZV</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20220601</creationdate><title>Distinct effects of different adjuvants in the mouse model of allergic airway inflammation</title><author>Shin, Hyun Soo ; Chun, Hye Rin ; Na, Hye Young ; Sohn, Moah ; Ryu, Seul Hye ; Choi, Wanho ; In, BaoqingHyunju ; Park, Ji Soo ; Park, Sejung ; Park, Chae Gyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-ad2bf54732d4649fe7358d5e93b15de7f1e7dd34646408cfccf9a31b9923cc733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adjuvants</topic><topic>Adjuvants, Immunologic</topic><topic>Animal models</topic><topic>Animals</topic><topic>Asthma</topic><topic>Asthma - etiology</topic><topic>Bronchoalveolar Lavage Fluid</topic><topic>Bronchus</topic><topic>Cytokines</topic><topic>Disease Models, Animal</topic><topic>Flow cytometry</topic><topic>Genotype & phenotype</topic><topic>Helper cells</topic><topic>Humans</topic><topic>Immunity, Innate</topic><topic>Inflammation</topic><topic>Inflammatory diseases</topic><topic>Lavage</topic><topic>Leukocytes (eosinophilic)</topic><topic>Leukocytes (neutrophilic)</topic><topic>Lipopolysaccharides</topic><topic>Lung - pathology</topic><topic>Lymph nodes</topic><topic>Lymphocytes - metabolism</topic><topic>Lymphocytes T</topic><topic>Lymphoid cells</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Ovalbumin</topic><topic>Papain</topic><topic>Papain - metabolism</topic><topic>Phenotypes</topic><topic>Respiratory tract diseases</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shin, Hyun Soo</creatorcontrib><creatorcontrib>Chun, Hye Rin</creatorcontrib><creatorcontrib>Na, Hye Young</creatorcontrib><creatorcontrib>Sohn, Moah</creatorcontrib><creatorcontrib>Ryu, Seul Hye</creatorcontrib><creatorcontrib>Choi, Wanho</creatorcontrib><creatorcontrib>In, BaoqingHyunju</creatorcontrib><creatorcontrib>Park, Ji Soo</creatorcontrib><creatorcontrib>Park, Sejung</creatorcontrib><creatorcontrib>Park, Chae Gyu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>East & South Asia Database</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Asian Pacific journal of allergy and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shin, Hyun Soo</au><au>Chun, Hye Rin</au><au>Na, Hye Young</au><au>Sohn, Moah</au><au>Ryu, Seul Hye</au><au>Choi, Wanho</au><au>In, BaoqingHyunju</au><au>Park, Ji Soo</au><au>Park, Sejung</au><au>Park, Chae Gyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distinct effects of different adjuvants in the mouse model of allergic airway inflammation</atitle><jtitle>Asian Pacific journal of allergy and immunology</jtitle><addtitle>Asian Pac J Allergy Immunol</addtitle><date>2022-06-01</date><risdate>2022</risdate><volume>40</volume><issue>2</issue><spage>111</spage><epage>120</epage><pages>111-120</pages><issn>0125-877X</issn><eissn>2228-8694</eissn><abstract>Allergic asthma was typically considered as an inflammatory disease mediated by type 2 immunity. However, recent studies revealed that asthma is a complex disease displaying a variety of phenotypes and endotypes.
We examined cellular phenotypes in the mouse model of allergic asthma sensitized with different adjuvants. The aim of our study was to determine immunologic cellular characteristics in mouse asthma models induced by ovalbumin (OVA) and a variety of adjuvants.
Mice were sensitized intraperitoneally with the admixture of OVA and various adjuvants such as Alhydrogel (alum), papain, lipopolysaccharide (LPS), or CpG, and subsequently challenged with OVA intranasally. The cells in bronchoalveolar lavage (BAL) fluid, lung, and mediastinal lymph node (mLN) were examined by flow cytometric analyses.
In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group. Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node. In the lung, the number of T-bet+ ILCs was highest in the papain group whereas the number of IFN-γ+ Th cells was highest in the CpG group.
Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.</abstract><cop>Thailand</cop><pub>The Allergy and Immunology Society</pub><pmid>32563228</pmid><doi>10.12932/AP-301219-0729</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0125-877X |
| ispartof | Asian Pacific journal of allergy and immunology, 2022-06, Vol.40 (2), p.111-120 |
| issn | 0125-877X 2228-8694 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2415836516 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adjuvants Adjuvants, Immunologic Animal models Animals Asthma Asthma - etiology Bronchoalveolar Lavage Fluid Bronchus Cytokines Disease Models, Animal Flow cytometry Genotype & phenotype Helper cells Humans Immunity, Innate Inflammation Inflammatory diseases Lavage Leukocytes (eosinophilic) Leukocytes (neutrophilic) Lipopolysaccharides Lung - pathology Lymph nodes Lymphocytes - metabolism Lymphocytes T Lymphoid cells Mice Mice, Inbred BALB C Ovalbumin Papain Papain - metabolism Phenotypes Respiratory tract diseases γ-Interferon |
| title | Distinct effects of different adjuvants in the mouse model of allergic airway inflammation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-12T11%3A22%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Distinct%20effects%20of%20different%20adjuvants%20in%20the%20mouse%20model%20of%20allergic%20airway%20inflammation&rft.jtitle=Asian%20Pacific%20journal%20of%20allergy%20and%20immunology&rft.au=Shin,%20Hyun%20Soo&rft.date=2022-06-01&rft.volume=40&rft.issue=2&rft.spage=111&rft.epage=120&rft.pages=111-120&rft.issn=0125-877X&rft.eissn=2228-8694&rft_id=info:doi/10.12932/AP-301219-0729&rft_dat=%3Cproquest_cross%3E2672062163%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2672062163&rft_id=info:pmid/32563228&rfr_iscdi=true |