Investigation of On-Farm Transmission Routes for Contamination of Dairy Cows with Top 7 Escherichia coli O-Serogroups
Shiga toxin-producing Escherichia coli (STEC) are foodborne bacterial pathogens, with cattle a significant reservoir for human infection. This study evaluated environmental reservoirs, intermediate hosts and key pathways that could drive the presence of Top 7 STEC (O157:H7, O26, O45, O103, O111, O12...
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| Veröffentlicht in: | Microbial ecology 2021, Vol.81 (1), p.67-77 |
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| creator | Rapp, D. Ross, C. M. Maclean, P. Cave, V. M. Brightwell, G. |
| description | Shiga toxin-producing
Escherichia coli
(STEC) are foodborne bacterial pathogens, with cattle a significant reservoir for human infection. This study evaluated environmental reservoirs, intermediate hosts and key pathways that could drive the presence of Top 7 STEC (O157:H7, O26, O45, O103, O111, O121 and O145) on pasture-based dairy herds, using molecular and culture-based methods. A total of 235 composite environmental samples (including soil, bedding, pasture, stock drinking water, bird droppings and flies and faecal samples of dairy animals) were collected from two dairy farms, with four sampling events on each farm. Molecular detection revealed O26, O45, O103 and O121 as the most common O-serogroups, with the greatest occurrence in dairy animal faeces (> 91%), environments freshly contaminated with faeces (> 73%) and birds and flies (> 71%). STEC (79 isolates) were a minor population within the target O-serogroups in all sample types but were widespread in the farm environment in the summer samplings. Phylogenetic analysis of whole genome sequence data targeting single nucleotide polymorphisms revealed the presence of several clonal strains on a farm; a single STEC clonal strain could be found in several sample types concurrently, indicating the existence of more than one possible route for transmission to dairy animals and a high rate of transmission of STEC between dairy animals and wildlife. Overall, the findings improved the understanding of the ecology of the Top 7 STEC in open farm environments, which is required to develop on-farm intervention strategies controlling these zoonoses. |
| doi_str_mv | 10.1007/s00248-020-01542-5 |
| format | Article |
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Escherichia coli
(STEC) are foodborne bacterial pathogens, with cattle a significant reservoir for human infection. This study evaluated environmental reservoirs, intermediate hosts and key pathways that could drive the presence of Top 7 STEC (O157:H7, O26, O45, O103, O111, O121 and O145) on pasture-based dairy herds, using molecular and culture-based methods. A total of 235 composite environmental samples (including soil, bedding, pasture, stock drinking water, bird droppings and flies and faecal samples of dairy animals) were collected from two dairy farms, with four sampling events on each farm. Molecular detection revealed O26, O45, O103 and O121 as the most common O-serogroups, with the greatest occurrence in dairy animal faeces (> 91%), environments freshly contaminated with faeces (> 73%) and birds and flies (> 71%). STEC (79 isolates) were a minor population within the target O-serogroups in all sample types but were widespread in the farm environment in the summer samplings. Phylogenetic analysis of whole genome sequence data targeting single nucleotide polymorphisms revealed the presence of several clonal strains on a farm; a single STEC clonal strain could be found in several sample types concurrently, indicating the existence of more than one possible route for transmission to dairy animals and a high rate of transmission of STEC between dairy animals and wildlife. Overall, the findings improved the understanding of the ecology of the Top 7 STEC in open farm environments, which is required to develop on-farm intervention strategies controlling these zoonoses.</description><identifier>ISSN: 0095-3628</identifier><identifier>EISSN: 1432-184X</identifier><identifier>DOI: 10.1007/s00248-020-01542-5</identifier><identifier>PMID: 32561945</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Bedding ; Biomedical and Life Sciences ; Birds ; Contamination ; Dairy cattle ; Dairy farming ; Dairy farms ; Drinking water ; E coli ; Ecology ; Environmental Microbiology ; Escherichia coli ; Faeces ; Farms ; Feces ; Genomes ; Geoecology/Natural Processes ; Hosts ; Life Sciences ; Microbial Ecology ; Microbiological strains ; Microbiology ; Nature Conservation ; Nucleotide sequence ; Nucleotides ; Pasture ; Pathogens ; Phylogeny ; Shiga toxin ; Single-nucleotide polymorphism ; Soil ; Soil contamination ; Toxins ; Water Quality/Water Pollution ; Wildlife</subject><ispartof>Microbial ecology, 2021, Vol.81 (1), p.67-77</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-d8ccf04fcec19f5e02d4dd3fe63cd8f6085aca776285376aa82f6ff2972139783</citedby><cites>FETCH-LOGICAL-c375t-d8ccf04fcec19f5e02d4dd3fe63cd8f6085aca776285376aa82f6ff2972139783</cites><orcidid>0000-0001-6763-4292 ; 0000-0002-1020-9014 ; 0000-0002-3980-4108 ; 0000-0001-8898-8276</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00248-020-01542-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00248-020-01542-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32561945$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rapp, D.</creatorcontrib><creatorcontrib>Ross, C. M.</creatorcontrib><creatorcontrib>Maclean, P.</creatorcontrib><creatorcontrib>Cave, V. M.</creatorcontrib><creatorcontrib>Brightwell, G.</creatorcontrib><title>Investigation of On-Farm Transmission Routes for Contamination of Dairy Cows with Top 7 Escherichia coli O-Serogroups</title><title>Microbial ecology</title><addtitle>Microb Ecol</addtitle><addtitle>Microb Ecol</addtitle><description>Shiga toxin-producing
Escherichia coli
(STEC) are foodborne bacterial pathogens, with cattle a significant reservoir for human infection. This study evaluated environmental reservoirs, intermediate hosts and key pathways that could drive the presence of Top 7 STEC (O157:H7, O26, O45, O103, O111, O121 and O145) on pasture-based dairy herds, using molecular and culture-based methods. A total of 235 composite environmental samples (including soil, bedding, pasture, stock drinking water, bird droppings and flies and faecal samples of dairy animals) were collected from two dairy farms, with four sampling events on each farm. Molecular detection revealed O26, O45, O103 and O121 as the most common O-serogroups, with the greatest occurrence in dairy animal faeces (> 91%), environments freshly contaminated with faeces (> 73%) and birds and flies (> 71%). STEC (79 isolates) were a minor population within the target O-serogroups in all sample types but were widespread in the farm environment in the summer samplings. Phylogenetic analysis of whole genome sequence data targeting single nucleotide polymorphisms revealed the presence of several clonal strains on a farm; a single STEC clonal strain could be found in several sample types concurrently, indicating the existence of more than one possible route for transmission to dairy animals and a high rate of transmission of STEC between dairy animals and wildlife. Overall, the findings improved the understanding of the ecology of the Top 7 STEC in open farm environments, which is required to develop on-farm intervention strategies controlling these zoonoses.</description><subject>Animals</subject><subject>Bedding</subject><subject>Biomedical and Life Sciences</subject><subject>Birds</subject><subject>Contamination</subject><subject>Dairy cattle</subject><subject>Dairy farming</subject><subject>Dairy farms</subject><subject>Drinking water</subject><subject>E coli</subject><subject>Ecology</subject><subject>Environmental Microbiology</subject><subject>Escherichia coli</subject><subject>Faeces</subject><subject>Farms</subject><subject>Feces</subject><subject>Genomes</subject><subject>Geoecology/Natural Processes</subject><subject>Hosts</subject><subject>Life Sciences</subject><subject>Microbial Ecology</subject><subject>Microbiological strains</subject><subject>Microbiology</subject><subject>Nature Conservation</subject><subject>Nucleotide sequence</subject><subject>Nucleotides</subject><subject>Pasture</subject><subject>Pathogens</subject><subject>Phylogeny</subject><subject>Shiga toxin</subject><subject>Single-nucleotide polymorphism</subject><subject>Soil</subject><subject>Soil contamination</subject><subject>Toxins</subject><subject>Water Quality/Water Pollution</subject><subject>Wildlife</subject><issn>0095-3628</issn><issn>1432-184X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU9LXDEUxYO06FT7BVxIoJtu0uZ_8pYy1SoIAzqCu5DmJTORecmYvFfx2zc6aqGLrgI3v3PuPRwAjgn-RjBW3yvGlGuEKUaYCE6R2AMzwhlFRPO7D2CGcScQk1QfgE-13mNMlKRsHxwwKiTpuJiB6TL99nWMKzvGnGAOcJHQuS0DXBab6hBrfZ5f52n0FYZc4Dyn0Q4xvQt-2Fie2vixwsc4ruEyb6GCZ9WtfYluHS10eRPhAt34klclT9t6BD4Gu6n-8-t7CG7Pz5bzC3S1-Hk5P71Cjikxol47FzAPzjvSBeEx7Xnfs-Alc70OEmthnVUtlBZMSWs1DTIE2ilKWKc0OwRfd77bkh-mltO0QM5vNjb5PFVDOREMC8pVQ7_8g97nqaR2XaPaBsFkJxtFd5Qrudbig9mWONjyZAg2z6WYXSmmlWJeSjGiiU5eradfg-_fJW8tNIDtgNq-0sqXv7v_Y_sHvyaXyA</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Rapp, D.</creator><creator>Ross, C. 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M.</au><au>Maclean, P.</au><au>Cave, V. M.</au><au>Brightwell, G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of On-Farm Transmission Routes for Contamination of Dairy Cows with Top 7 Escherichia coli O-Serogroups</atitle><jtitle>Microbial ecology</jtitle><stitle>Microb Ecol</stitle><addtitle>Microb Ecol</addtitle><date>2021</date><risdate>2021</risdate><volume>81</volume><issue>1</issue><spage>67</spage><epage>77</epage><pages>67-77</pages><issn>0095-3628</issn><eissn>1432-184X</eissn><abstract>Shiga toxin-producing
Escherichia coli
(STEC) are foodborne bacterial pathogens, with cattle a significant reservoir for human infection. This study evaluated environmental reservoirs, intermediate hosts and key pathways that could drive the presence of Top 7 STEC (O157:H7, O26, O45, O103, O111, O121 and O145) on pasture-based dairy herds, using molecular and culture-based methods. A total of 235 composite environmental samples (including soil, bedding, pasture, stock drinking water, bird droppings and flies and faecal samples of dairy animals) were collected from two dairy farms, with four sampling events on each farm. Molecular detection revealed O26, O45, O103 and O121 as the most common O-serogroups, with the greatest occurrence in dairy animal faeces (> 91%), environments freshly contaminated with faeces (> 73%) and birds and flies (> 71%). STEC (79 isolates) were a minor population within the target O-serogroups in all sample types but were widespread in the farm environment in the summer samplings. Phylogenetic analysis of whole genome sequence data targeting single nucleotide polymorphisms revealed the presence of several clonal strains on a farm; a single STEC clonal strain could be found in several sample types concurrently, indicating the existence of more than one possible route for transmission to dairy animals and a high rate of transmission of STEC between dairy animals and wildlife. Overall, the findings improved the understanding of the ecology of the Top 7 STEC in open farm environments, which is required to develop on-farm intervention strategies controlling these zoonoses.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>32561945</pmid><doi>10.1007/s00248-020-01542-5</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-6763-4292</orcidid><orcidid>https://orcid.org/0000-0002-1020-9014</orcidid><orcidid>https://orcid.org/0000-0002-3980-4108</orcidid><orcidid>https://orcid.org/0000-0001-8898-8276</orcidid></addata></record> |
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| ispartof | Microbial ecology, 2021, Vol.81 (1), p.67-77 |
| issn | 0095-3628 1432-184X |
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| source | SpringerNature Journals |
| subjects | Animals Bedding Biomedical and Life Sciences Birds Contamination Dairy cattle Dairy farming Dairy farms Drinking water E coli Ecology Environmental Microbiology Escherichia coli Faeces Farms Feces Genomes Geoecology/Natural Processes Hosts Life Sciences Microbial Ecology Microbiological strains Microbiology Nature Conservation Nucleotide sequence Nucleotides Pasture Pathogens Phylogeny Shiga toxin Single-nucleotide polymorphism Soil Soil contamination Toxins Water Quality/Water Pollution Wildlife |
| title | Investigation of On-Farm Transmission Routes for Contamination of Dairy Cows with Top 7 Escherichia coli O-Serogroups |
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