Optimized low pH formulation of niacinamide enhances induction of autophagy marker ATG5 gene expression and protein levels in human epidermal keratinocytes

Background Macromolecules in skin cells are damaged when exposed to environmental stressors, leading to disrupted cellular function and homeostasis. While epidermal turnover can eliminate some of this damage, autophagy can rapidly remove these defective components. Niacinamide (Nam) is known to indu...

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Veröffentlicht in:Journal of the European Academy of Dermatology and Venereology 2020-06, Vol.34 (S3), p.3-11
Hauptverfasser: Oblong, J.E., DeAngelis, Y.M., Jarrold, B.B., Bierman, J.C., Rovito, H.A., Vires, L., Fang, B., Laughlin, T., Zhao, W., Hartman, S.M., Kainkaryam, R., Adams, R., Sherrill, J.D., Hakozaki, T.
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container_end_page 11
container_issue S3
container_start_page 3
container_title Journal of the European Academy of Dermatology and Venereology
container_volume 34
creator Oblong, J.E.
DeAngelis, Y.M.
Jarrold, B.B.
Bierman, J.C.
Rovito, H.A.
Vires, L.
Fang, B.
Laughlin, T.
Zhao, W.
Hartman, S.M.
Kainkaryam, R.
Adams, R.
Sherrill, J.D.
Hakozaki, T.
description Background Macromolecules in skin cells are damaged when exposed to environmental stressors, leading to disrupted cellular function and homeostasis. While epidermal turnover can eliminate some of this damage, autophagy can rapidly remove these defective components. Niacinamide (Nam) is known to induce autophagy and optimizing formulations to maximize this response could provide improved homeostasis in stressed skin. Objective To determine (i) whether Nam can induce autophagy related 5 (ATG5), an autophagy marker, in human keratinocytes and (ii) whether optimized low pH Nam formulations can enhance the response in 3D skin models. Methods Human keratinocytes treated with Nam were evaluated for autophagosome accumulation and induction of ATG5 by gene expression, immunoblotting and immune‐fluorescence microscopy. 3D skin equivalents were topically treated with Nam formulations at pH 5.8 and 3.8. Gene expression profiling and immunoblot analysis of ATG5 were performed. Results Nam treatment of keratinocytes led to an accumulation of autophagosomes with a maximal signal at 48 h. Gene expression of ATG5 was induced by Nam, and immunoblots stained for ATG5 showed a significant increase after 6 h of treatment. Gene expression profiling of 3D epidermal skin equivalents treated with Nam at pH 3.8 showed stronger induction of autophagy‐related genes, including ATG5, compared with pH 5.8 formulas. Enrichment for gene ontology terms on autophagy showed an increased linkage with Nam formulas at pH 3.8. Conclusions We found that Nam induces autophagosome accumulation and ATG5 levels in keratinocytes. We also discovered that a Nam formulation at pH 3.8 can further increase levels of ATG5 in 3D skin models when compared to Nam at pH 5.8. These data support that Nam can induce autophagy in keratinocytes and formulations at pH 3.8 can enhance the impact. We hypothesize that optimized formulations at pH 3.8 can improve skin ageing appearance via autophagy induction.
doi_str_mv 10.1111/jdv.16582
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While epidermal turnover can eliminate some of this damage, autophagy can rapidly remove these defective components. Niacinamide (Nam) is known to induce autophagy and optimizing formulations to maximize this response could provide improved homeostasis in stressed skin. Objective To determine (i) whether Nam can induce autophagy related 5 (ATG5), an autophagy marker, in human keratinocytes and (ii) whether optimized low pH Nam formulations can enhance the response in 3D skin models. Methods Human keratinocytes treated with Nam were evaluated for autophagosome accumulation and induction of ATG5 by gene expression, immunoblotting and immune‐fluorescence microscopy. 3D skin equivalents were topically treated with Nam formulations at pH 5.8 and 3.8. Gene expression profiling and immunoblot analysis of ATG5 were performed. Results Nam treatment of keratinocytes led to an accumulation of autophagosomes with a maximal signal at 48 h. Gene expression of ATG5 was induced by Nam, and immunoblots stained for ATG5 showed a significant increase after 6 h of treatment. Gene expression profiling of 3D epidermal skin equivalents treated with Nam at pH 3.8 showed stronger induction of autophagy‐related genes, including ATG5, compared with pH 5.8 formulas. Enrichment for gene ontology terms on autophagy showed an increased linkage with Nam formulas at pH 3.8. Conclusions We found that Nam induces autophagosome accumulation and ATG5 levels in keratinocytes. We also discovered that a Nam formulation at pH 3.8 can further increase levels of ATG5 in 3D skin models when compared to Nam at pH 5.8. These data support that Nam can induce autophagy in keratinocytes and formulations at pH 3.8 can enhance the impact. 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While epidermal turnover can eliminate some of this damage, autophagy can rapidly remove these defective components. Niacinamide (Nam) is known to induce autophagy and optimizing formulations to maximize this response could provide improved homeostasis in stressed skin. Objective To determine (i) whether Nam can induce autophagy related 5 (ATG5), an autophagy marker, in human keratinocytes and (ii) whether optimized low pH Nam formulations can enhance the response in 3D skin models. Methods Human keratinocytes treated with Nam were evaluated for autophagosome accumulation and induction of ATG5 by gene expression, immunoblotting and immune‐fluorescence microscopy. 3D skin equivalents were topically treated with Nam formulations at pH 5.8 and 3.8. Gene expression profiling and immunoblot analysis of ATG5 were performed. Results Nam treatment of keratinocytes led to an accumulation of autophagosomes with a maximal signal at 48 h. Gene expression of ATG5 was induced by Nam, and immunoblots stained for ATG5 showed a significant increase after 6 h of treatment. Gene expression profiling of 3D epidermal skin equivalents treated with Nam at pH 3.8 showed stronger induction of autophagy‐related genes, including ATG5, compared with pH 5.8 formulas. Enrichment for gene ontology terms on autophagy showed an increased linkage with Nam formulas at pH 3.8. Conclusions We found that Nam induces autophagosome accumulation and ATG5 levels in keratinocytes. We also discovered that a Nam formulation at pH 3.8 can further increase levels of ATG5 in 3D skin models when compared to Nam at pH 5.8. These data support that Nam can induce autophagy in keratinocytes and formulations at pH 3.8 can enhance the impact. We hypothesize that optimized formulations at pH 3.8 can improve skin ageing appearance via autophagy induction.</description><subject>Autophagy</subject><subject>Autophagy-Related Protein 5 - genetics</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Keratinocytes - metabolism</subject><subject>Niacinamide</subject><issn>0926-9959</issn><issn>1468-3083</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kbtSxCAUhhlHR9dL4Qs4lFpEuSQsKR3vjjM2apsh5MRFCURI1PVVfFlZV-2koeA7H-ecH6FdSg5pOkdPzeshFYVkK2hCcyEzTiRfRRNSMpGVZVFuoM0YnwghlBZyHW1wVhRTScQEfd72g-nMBzTY-jfcX-LWh260ajDeYd9iZ5Q2TnWmAQxuppyGiI1rRv1LqHHw_Uw9znGnwjMEfHx3UeBHcKngvQ8Q4wJUrsF98AMYhy28gl1Y8GzslMPQJ3volMWpPP3svJ4PELfRWqtshJ2fewvdn5_dnVxmN7cXVyfHN5nmgrBMMwl0qoXKmWZ1Xbc1pJF5yVtGARRLjxx404oyB0ql0FJKUjdyWmuaEwF8C-0vvam_lxHiUHUmarBWOfBjrFhOC1aWLCcJPViiOvgYA7RVH0wae15RUi2yqFIW1XcWid370Y51B80f-bv8BBwtgTdjYf6_qbo-fVgqvwBe35cZ</recordid><startdate>202006</startdate><enddate>202006</enddate><creator>Oblong, J.E.</creator><creator>DeAngelis, Y.M.</creator><creator>Jarrold, B.B.</creator><creator>Bierman, J.C.</creator><creator>Rovito, H.A.</creator><creator>Vires, L.</creator><creator>Fang, B.</creator><creator>Laughlin, T.</creator><creator>Zhao, W.</creator><creator>Hartman, S.M.</creator><creator>Kainkaryam, R.</creator><creator>Adams, R.</creator><creator>Sherrill, J.D.</creator><creator>Hakozaki, T.</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202006</creationdate><title>Optimized low pH formulation of niacinamide enhances induction of autophagy marker ATG5 gene expression and protein levels in human epidermal keratinocytes</title><author>Oblong, J.E. ; DeAngelis, Y.M. ; Jarrold, B.B. ; Bierman, J.C. ; Rovito, H.A. ; Vires, L. ; Fang, B. ; Laughlin, T. ; Zhao, W. ; Hartman, S.M. ; Kainkaryam, R. ; Adams, R. ; Sherrill, J.D. ; Hakozaki, T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3602-c28e17c6a42c2bbbfbe959393f21eea2e173e3df694e1186c8880bd87bc1406e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Autophagy</topic><topic>Autophagy-Related Protein 5 - genetics</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Keratinocytes - metabolism</topic><topic>Niacinamide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oblong, J.E.</creatorcontrib><creatorcontrib>DeAngelis, Y.M.</creatorcontrib><creatorcontrib>Jarrold, B.B.</creatorcontrib><creatorcontrib>Bierman, J.C.</creatorcontrib><creatorcontrib>Rovito, H.A.</creatorcontrib><creatorcontrib>Vires, L.</creatorcontrib><creatorcontrib>Fang, B.</creatorcontrib><creatorcontrib>Laughlin, T.</creatorcontrib><creatorcontrib>Zhao, W.</creatorcontrib><creatorcontrib>Hartman, S.M.</creatorcontrib><creatorcontrib>Kainkaryam, R.</creatorcontrib><creatorcontrib>Adams, R.</creatorcontrib><creatorcontrib>Sherrill, J.D.</creatorcontrib><creatorcontrib>Hakozaki, T.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the European Academy of Dermatology and Venereology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oblong, J.E.</au><au>DeAngelis, Y.M.</au><au>Jarrold, B.B.</au><au>Bierman, J.C.</au><au>Rovito, H.A.</au><au>Vires, L.</au><au>Fang, B.</au><au>Laughlin, T.</au><au>Zhao, W.</au><au>Hartman, S.M.</au><au>Kainkaryam, R.</au><au>Adams, R.</au><au>Sherrill, J.D.</au><au>Hakozaki, T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimized low pH formulation of niacinamide enhances induction of autophagy marker ATG5 gene expression and protein levels in human epidermal keratinocytes</atitle><jtitle>Journal of the European Academy of Dermatology and Venereology</jtitle><addtitle>J Eur Acad Dermatol Venereol</addtitle><date>2020-06</date><risdate>2020</risdate><volume>34</volume><issue>S3</issue><spage>3</spage><epage>11</epage><pages>3-11</pages><issn>0926-9959</issn><eissn>1468-3083</eissn><abstract>Background Macromolecules in skin cells are damaged when exposed to environmental stressors, leading to disrupted cellular function and homeostasis. While epidermal turnover can eliminate some of this damage, autophagy can rapidly remove these defective components. Niacinamide (Nam) is known to induce autophagy and optimizing formulations to maximize this response could provide improved homeostasis in stressed skin. Objective To determine (i) whether Nam can induce autophagy related 5 (ATG5), an autophagy marker, in human keratinocytes and (ii) whether optimized low pH Nam formulations can enhance the response in 3D skin models. Methods Human keratinocytes treated with Nam were evaluated for autophagosome accumulation and induction of ATG5 by gene expression, immunoblotting and immune‐fluorescence microscopy. 3D skin equivalents were topically treated with Nam formulations at pH 5.8 and 3.8. Gene expression profiling and immunoblot analysis of ATG5 were performed. Results Nam treatment of keratinocytes led to an accumulation of autophagosomes with a maximal signal at 48 h. Gene expression of ATG5 was induced by Nam, and immunoblots stained for ATG5 showed a significant increase after 6 h of treatment. Gene expression profiling of 3D epidermal skin equivalents treated with Nam at pH 3.8 showed stronger induction of autophagy‐related genes, including ATG5, compared with pH 5.8 formulas. Enrichment for gene ontology terms on autophagy showed an increased linkage with Nam formulas at pH 3.8. Conclusions We found that Nam induces autophagosome accumulation and ATG5 levels in keratinocytes. We also discovered that a Nam formulation at pH 3.8 can further increase levels of ATG5 in 3D skin models when compared to Nam at pH 5.8. These data support that Nam can induce autophagy in keratinocytes and formulations at pH 3.8 can enhance the impact. We hypothesize that optimized formulations at pH 3.8 can improve skin ageing appearance via autophagy induction.</abstract><cop>England</cop><pmid>32557806</pmid><doi>10.1111/jdv.16582</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record>
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subjects Autophagy
Autophagy-Related Protein 5 - genetics
Gene Expression
Humans
Hydrogen-Ion Concentration
Keratinocytes - metabolism
Niacinamide
title Optimized low pH formulation of niacinamide enhances induction of autophagy marker ATG5 gene expression and protein levels in human epidermal keratinocytes
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