Investigation of promoter methylation of MCPH1 gene in circulating cell-free DNA of brain tumor patients
Introduction Despite advanced diagnostic and therapeutic techniques, many brain tumors are still diagnosed at high grades and, therefore finding novel molecular markers may assist in early detection and reducing brain tumors-related mortality rate. Owing to the previous reports on the importance of...
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| Veröffentlicht in: | Experimental brain research 2020-09, Vol.238 (9), p.1903-1909 |
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| description | Introduction
Despite advanced diagnostic and therapeutic techniques, many brain tumors are still diagnosed at high grades and, therefore finding novel molecular markers may assist in early detection and reducing brain tumors-related mortality rate. Owing to the previous reports on the importance of
MCPH1
gene in tumorigenesis, the present study was aimed to study the promoter methylation of
MCPH1
gene in paired circulating cell-free DNA (cfDNA) and tumor tissues of brain tumor patients.
Materials and methods
Fourteen fresh paired serum and tumor tissue samples in addition to 18 isolated serum samples were collected from patients affected by different grades of brain tumor. Genomic DNA and cfDNA was isolated from tissue and serum samples using QIAamp DNA Mini Kit Norgen Bioteck Kit, respectively. Methylation DNA immunoprecipitation Real-time polymerization chain reaction (MeDIP-Real-time PCR) was performed on isolated DNA samples using EpiQuik MeDIP Ultra Kit and specific primer pairs. cfDNA quantity was determined through Real-time PCR analysis using specific primer pairs designed for
GAPDH
gene.
Results
MCPH1
was methylated in 54% of cfDNA samples which was significantly associated with tumor grade, as well (
P
-value = 0.02). The methylation rate of
MCPH1
was found as 78% in the tissue samples which was meaningfully associated with tumor grade (
P
-value = 0.03). Moreover, methylation of the
MCPH1
gene was consistent in 57% of the same cfDNA and tissue samples. Methylation of
MCPH1
gene in neither tumor tissues nor cfDNA was not correlated with age and sex of the patients.
Discussion and conclusion
Due to the conformity of methylation of
MCPH1
gene in cfDNA and tissue samples in more than half of the enrolled patients, especially in higher grades of tumors, it seems that
MCPH1
promoter methylation could be a potential epimarker in not only detection of brain tumors but also in response to chemo- and radiotherapy which warranted further assessment. |
| doi_str_mv | 10.1007/s00221-020-05848-1 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2415291460</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A633014987</galeid><sourcerecordid>A633014987</sourcerecordid><originalsourceid>FETCH-LOGICAL-c507t-bc7933dd2c1b723c28cdbfb8012ad71317130ab1ef8cc79a03b5f01ae473db2b3</originalsourceid><addsrcrecordid>eNp9kttu1DAQhi0EoqXwAlygSEgILlJm7GSTvVwth65UDuJwbdnOJJsqsbe2g-jb43RLyyKELMvy-PtHM-OfsacIpwhQvQ4AnGMOHHIo66LO8R47xkLwHBEW99kxABZ5UePyiD0K4WK-igoesiPBy3JR8OqYbTf2B4XYdyr2zmauzXbejS6Sz0aK26vhNv5h_fkMs44sZb3NTO_NND_aLjM0DHnribI3H1czqr1KSJxG57NdYsjG8Jg9aNUQ6MnNecK-v3v7bX2Wn396v1mvznNTQhVzbaqlEE3DDeqKC8Nr0-hW14BcNRUKTBuURmprk1AFQpctoKKiEo3mWpywl_u8qY_LKbUmxz7MFSpLbgqSF1jyJRYLSOjzv9ALN3mbqkuUKIUQRV3dUZ0aSPa2ddErMyeVq4UQaabLa-r0H1RaDY29cZbaPsUPBK8OBImJ9DN2agpBbr5-OWRf_MFuSQ1xG9wwzV8TDkG-B413IXhq5c73o_JXEkHOnpF7z8jkGXntGYlJ9OxmDJMeqbmV_DZJAsQeCOnJduTv5vSftL8Aw_rIfg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2435333487</pqid></control><display><type>article</type><title>Investigation of promoter methylation of MCPH1 gene in circulating cell-free DNA of brain tumor patients</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Ghodsi, Marjan ; Shahmohammadi, Mohammadreza ; Modarressi, Mohammad Hossein ; Karami, Fatemeh</creator><creatorcontrib>Ghodsi, Marjan ; Shahmohammadi, Mohammadreza ; Modarressi, Mohammad Hossein ; Karami, Fatemeh</creatorcontrib><description>Introduction
Despite advanced diagnostic and therapeutic techniques, many brain tumors are still diagnosed at high grades and, therefore finding novel molecular markers may assist in early detection and reducing brain tumors-related mortality rate. Owing to the previous reports on the importance of
MCPH1
gene in tumorigenesis, the present study was aimed to study the promoter methylation of
MCPH1
gene in paired circulating cell-free DNA (cfDNA) and tumor tissues of brain tumor patients.
Materials and methods
Fourteen fresh paired serum and tumor tissue samples in addition to 18 isolated serum samples were collected from patients affected by different grades of brain tumor. Genomic DNA and cfDNA was isolated from tissue and serum samples using QIAamp DNA Mini Kit Norgen Bioteck Kit, respectively. Methylation DNA immunoprecipitation Real-time polymerization chain reaction (MeDIP-Real-time PCR) was performed on isolated DNA samples using EpiQuik MeDIP Ultra Kit and specific primer pairs. cfDNA quantity was determined through Real-time PCR analysis using specific primer pairs designed for
GAPDH
gene.
Results
MCPH1
was methylated in 54% of cfDNA samples which was significantly associated with tumor grade, as well (
P
-value = 0.02). The methylation rate of
MCPH1
was found as 78% in the tissue samples which was meaningfully associated with tumor grade (
P
-value = 0.03). Moreover, methylation of the
MCPH1
gene was consistent in 57% of the same cfDNA and tissue samples. Methylation of
MCPH1
gene in neither tumor tissues nor cfDNA was not correlated with age and sex of the patients.
Discussion and conclusion
Due to the conformity of methylation of
MCPH1
gene in cfDNA and tissue samples in more than half of the enrolled patients, especially in higher grades of tumors, it seems that
MCPH1
promoter methylation could be a potential epimarker in not only detection of brain tumors but also in response to chemo- and radiotherapy which warranted further assessment.</description><identifier>ISSN: 0014-4819</identifier><identifier>EISSN: 1432-1106</identifier><identifier>DOI: 10.1007/s00221-020-05848-1</identifier><identifier>PMID: 32556427</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analysis ; Biomarkers, Tumor ; Biomedical and Life Sciences ; Biomedicine ; Brain cancer ; Brain Neoplasms - genetics ; Brain tumors ; Cell Cycle Proteins ; Cell-Free Nucleic Acids - genetics ; Cytoskeletal Proteins - genetics ; Deoxyribonucleic acid ; DNA ; DNA Methylation ; DNA, Neoplasm ; Ethylenediaminetetraacetic acid ; Genes ; Genetic research ; Genomics ; Glyceraldehyde-3-phosphate dehydrogenase ; Health aspects ; Humans ; Immunoprecipitation ; Iran ; Methylation ; Mortality ; Neurology ; Neurosciences ; Polymerase chain reaction ; Polymerization ; Promoter Regions, Genetic ; Radiation therapy ; Research Article ; Tumorigenesis ; Tumors</subject><ispartof>Experimental brain research, 2020-09, Vol.238 (9), p.1903-1909</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>COPYRIGHT 2020 Springer</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c507t-bc7933dd2c1b723c28cdbfb8012ad71317130ab1ef8cc79a03b5f01ae473db2b3</citedby><cites>FETCH-LOGICAL-c507t-bc7933dd2c1b723c28cdbfb8012ad71317130ab1ef8cc79a03b5f01ae473db2b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00221-020-05848-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00221-020-05848-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32556427$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ghodsi, Marjan</creatorcontrib><creatorcontrib>Shahmohammadi, Mohammadreza</creatorcontrib><creatorcontrib>Modarressi, Mohammad Hossein</creatorcontrib><creatorcontrib>Karami, Fatemeh</creatorcontrib><title>Investigation of promoter methylation of MCPH1 gene in circulating cell-free DNA of brain tumor patients</title><title>Experimental brain research</title><addtitle>Exp Brain Res</addtitle><addtitle>Exp Brain Res</addtitle><description>Introduction
Despite advanced diagnostic and therapeutic techniques, many brain tumors are still diagnosed at high grades and, therefore finding novel molecular markers may assist in early detection and reducing brain tumors-related mortality rate. Owing to the previous reports on the importance of
MCPH1
gene in tumorigenesis, the present study was aimed to study the promoter methylation of
MCPH1
gene in paired circulating cell-free DNA (cfDNA) and tumor tissues of brain tumor patients.
Materials and methods
Fourteen fresh paired serum and tumor tissue samples in addition to 18 isolated serum samples were collected from patients affected by different grades of brain tumor. Genomic DNA and cfDNA was isolated from tissue and serum samples using QIAamp DNA Mini Kit Norgen Bioteck Kit, respectively. Methylation DNA immunoprecipitation Real-time polymerization chain reaction (MeDIP-Real-time PCR) was performed on isolated DNA samples using EpiQuik MeDIP Ultra Kit and specific primer pairs. cfDNA quantity was determined through Real-time PCR analysis using specific primer pairs designed for
GAPDH
gene.
Results
MCPH1
was methylated in 54% of cfDNA samples which was significantly associated with tumor grade, as well (
P
-value = 0.02). The methylation rate of
MCPH1
was found as 78% in the tissue samples which was meaningfully associated with tumor grade (
P
-value = 0.03). Moreover, methylation of the
MCPH1
gene was consistent in 57% of the same cfDNA and tissue samples. Methylation of
MCPH1
gene in neither tumor tissues nor cfDNA was not correlated with age and sex of the patients.
Discussion and conclusion
Due to the conformity of methylation of
MCPH1
gene in cfDNA and tissue samples in more than half of the enrolled patients, especially in higher grades of tumors, it seems that
MCPH1
promoter methylation could be a potential epimarker in not only detection of brain tumors but also in response to chemo- and radiotherapy which warranted further assessment.</description><subject>Analysis</subject><subject>Biomarkers, Tumor</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Brain cancer</subject><subject>Brain Neoplasms - genetics</subject><subject>Brain tumors</subject><subject>Cell Cycle Proteins</subject><subject>Cell-Free Nucleic Acids - genetics</subject><subject>Cytoskeletal Proteins - genetics</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Methylation</subject><subject>DNA, Neoplasm</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Genes</subject><subject>Genetic research</subject><subject>Genomics</subject><subject>Glyceraldehyde-3-phosphate dehydrogenase</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Immunoprecipitation</subject><subject>Iran</subject><subject>Methylation</subject><subject>Mortality</subject><subject>Neurology</subject><subject>Neurosciences</subject><subject>Polymerase chain reaction</subject><subject>Polymerization</subject><subject>Promoter Regions, Genetic</subject><subject>Radiation therapy</subject><subject>Research Article</subject><subject>Tumorigenesis</subject><subject>Tumors</subject><issn>0014-4819</issn><issn>1432-1106</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kttu1DAQhi0EoqXwAlygSEgILlJm7GSTvVwth65UDuJwbdnOJJsqsbe2g-jb43RLyyKELMvy-PtHM-OfsacIpwhQvQ4AnGMOHHIo66LO8R47xkLwHBEW99kxABZ5UePyiD0K4WK-igoesiPBy3JR8OqYbTf2B4XYdyr2zmauzXbejS6Sz0aK26vhNv5h_fkMs44sZb3NTO_NND_aLjM0DHnribI3H1czqr1KSJxG57NdYsjG8Jg9aNUQ6MnNecK-v3v7bX2Wn396v1mvznNTQhVzbaqlEE3DDeqKC8Nr0-hW14BcNRUKTBuURmprk1AFQpctoKKiEo3mWpywl_u8qY_LKbUmxz7MFSpLbgqSF1jyJRYLSOjzv9ALN3mbqkuUKIUQRV3dUZ0aSPa2ddErMyeVq4UQaabLa-r0H1RaDY29cZbaPsUPBK8OBImJ9DN2agpBbr5-OWRf_MFuSQ1xG9wwzV8TDkG-B413IXhq5c73o_JXEkHOnpF7z8jkGXntGYlJ9OxmDJMeqbmV_DZJAsQeCOnJduTv5vSftL8Aw_rIfg</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Ghodsi, Marjan</creator><creator>Shahmohammadi, Mohammadreza</creator><creator>Modarressi, Mohammad Hossein</creator><creator>Karami, Fatemeh</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>0-V</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>88J</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ALSLI</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M2R</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20200901</creationdate><title>Investigation of promoter methylation of MCPH1 gene in circulating cell-free DNA of brain tumor patients</title><author>Ghodsi, Marjan ; Shahmohammadi, Mohammadreza ; Modarressi, Mohammad Hossein ; Karami, Fatemeh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507t-bc7933dd2c1b723c28cdbfb8012ad71317130ab1ef8cc79a03b5f01ae473db2b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Analysis</topic><topic>Biomarkers, Tumor</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Brain cancer</topic><topic>Brain Neoplasms - genetics</topic><topic>Brain tumors</topic><topic>Cell Cycle Proteins</topic><topic>Cell-Free Nucleic Acids - genetics</topic><topic>Cytoskeletal Proteins - genetics</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Methylation</topic><topic>DNA, Neoplasm</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Genes</topic><topic>Genetic research</topic><topic>Genomics</topic><topic>Glyceraldehyde-3-phosphate dehydrogenase</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Immunoprecipitation</topic><topic>Iran</topic><topic>Methylation</topic><topic>Mortality</topic><topic>Neurology</topic><topic>Neurosciences</topic><topic>Polymerase chain reaction</topic><topic>Polymerization</topic><topic>Promoter Regions, Genetic</topic><topic>Radiation therapy</topic><topic>Research Article</topic><topic>Tumorigenesis</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ghodsi, Marjan</creatorcontrib><creatorcontrib>Shahmohammadi, Mohammadreza</creatorcontrib><creatorcontrib>Modarressi, Mohammad Hossein</creatorcontrib><creatorcontrib>Karami, Fatemeh</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Social Sciences Premium Collection</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>Social Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Social Science Premium Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Psychology Database</collection><collection>Social Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ghodsi, Marjan</au><au>Shahmohammadi, Mohammadreza</au><au>Modarressi, Mohammad Hossein</au><au>Karami, Fatemeh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of promoter methylation of MCPH1 gene in circulating cell-free DNA of brain tumor patients</atitle><jtitle>Experimental brain research</jtitle><stitle>Exp Brain Res</stitle><addtitle>Exp Brain Res</addtitle><date>2020-09-01</date><risdate>2020</risdate><volume>238</volume><issue>9</issue><spage>1903</spage><epage>1909</epage><pages>1903-1909</pages><issn>0014-4819</issn><eissn>1432-1106</eissn><abstract>Introduction
Despite advanced diagnostic and therapeutic techniques, many brain tumors are still diagnosed at high grades and, therefore finding novel molecular markers may assist in early detection and reducing brain tumors-related mortality rate. Owing to the previous reports on the importance of
MCPH1
gene in tumorigenesis, the present study was aimed to study the promoter methylation of
MCPH1
gene in paired circulating cell-free DNA (cfDNA) and tumor tissues of brain tumor patients.
Materials and methods
Fourteen fresh paired serum and tumor tissue samples in addition to 18 isolated serum samples were collected from patients affected by different grades of brain tumor. Genomic DNA and cfDNA was isolated from tissue and serum samples using QIAamp DNA Mini Kit Norgen Bioteck Kit, respectively. Methylation DNA immunoprecipitation Real-time polymerization chain reaction (MeDIP-Real-time PCR) was performed on isolated DNA samples using EpiQuik MeDIP Ultra Kit and specific primer pairs. cfDNA quantity was determined through Real-time PCR analysis using specific primer pairs designed for
GAPDH
gene.
Results
MCPH1
was methylated in 54% of cfDNA samples which was significantly associated with tumor grade, as well (
P
-value = 0.02). The methylation rate of
MCPH1
was found as 78% in the tissue samples which was meaningfully associated with tumor grade (
P
-value = 0.03). Moreover, methylation of the
MCPH1
gene was consistent in 57% of the same cfDNA and tissue samples. Methylation of
MCPH1
gene in neither tumor tissues nor cfDNA was not correlated with age and sex of the patients.
Discussion and conclusion
Due to the conformity of methylation of
MCPH1
gene in cfDNA and tissue samples in more than half of the enrolled patients, especially in higher grades of tumors, it seems that
MCPH1
promoter methylation could be a potential epimarker in not only detection of brain tumors but also in response to chemo- and radiotherapy which warranted further assessment.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32556427</pmid><doi>10.1007/s00221-020-05848-1</doi><tpages>7</tpages></addata></record> |
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| ispartof | Experimental brain research, 2020-09, Vol.238 (9), p.1903-1909 |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Analysis Biomarkers, Tumor Biomedical and Life Sciences Biomedicine Brain cancer Brain Neoplasms - genetics Brain tumors Cell Cycle Proteins Cell-Free Nucleic Acids - genetics Cytoskeletal Proteins - genetics Deoxyribonucleic acid DNA DNA Methylation DNA, Neoplasm Ethylenediaminetetraacetic acid Genes Genetic research Genomics Glyceraldehyde-3-phosphate dehydrogenase Health aspects Humans Immunoprecipitation Iran Methylation Mortality Neurology Neurosciences Polymerase chain reaction Polymerization Promoter Regions, Genetic Radiation therapy Research Article Tumorigenesis Tumors |
| title | Investigation of promoter methylation of MCPH1 gene in circulating cell-free DNA of brain tumor patients |
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