Effects of Hysteroscopic and Uterine Body Insemination on the Presence of Selected Immune Cells in the Equine Endometrium
The effects of standard uterine body and hysteroscopic insemination on endometrial health were investigated. For this purpose, 33 mares were assigned to five different protocols: control (no insemination; n = 7), sham AI (sham uterine body insemination; n = 6), sham HysAI (sham hysteroscopic insemin...
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| Veröffentlicht in: | Journal of equine veterinary science 2020-07, Vol.90, p.103023-103023, Article 103023 |
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| creator | Köhne, Martin Mönnig, Franziska Papin, Johanna Schöniger, Sandra Tönissen, Anna Rohn, Karl Martinsson, Gunilla Schoon, Heinz-Adolf Sieme, Harald |
| description | The effects of standard uterine body and hysteroscopic insemination on endometrial health were investigated. For this purpose, 33 mares were assigned to five different protocols: control (no insemination; n = 7), sham AI (sham uterine body insemination; n = 6), sham HysAI (sham hysteroscopic insemination; n = 7), standard AI (standard uterine body insemination, 300 × 106 progressively motile sperms (PMS); n = 7) and HysAI (hysteroscopic insemination, 100 × 106 PMS; n = 6). Sampling included uterine swabbing for microbiological examination, cytology for determination of polymorphonuclear neutrophils (PMNs) in the uterus, and endometrial biopsy collection for histology and characterization of endometrial immune cells on day 18 after ovulation (B1) as well as 8–10 hours (B2, day 20) and 72 hours after insemination (B3, day 23). Microbial contamination increased throughout the experiment in the sham insemination groups. Significant effects (P < .05) over time were detected for PMNs (cytology: sham HysAI, standard AI, and HysAI; histology: standard AI and HysAI), macrophages (immunohistochemistry: standard AI and HysAI) and T cells (immunohistochemistry: standard AI), showing an increase at B2 and a subsequent decrease toward baseline levels at B3. At B2, significant differences (P < .05) existed for PMNs (mean ± SEM) between control (1.3 ± 1.9%) and sham AI (2.2 ± 2.7%) versus standard AI (12.2 ± 4.7%) and for macrophages between control (4.1 ± 3.5%) and sham AI (2.5 ± 1.3%) versus standard AI (25.4 ± 15.8%). Thus, the cellular immune response of the endometrium depends on sperm deposition in the uterus and does not differ between hysteroscopic and standard uterine body insemination.
•Effects of standard uterine body and hysteroscopic insemination on endometrial health were investigated by cytology, histology, immunohistochemistry, and microbiology.•Histological and immunohistochemical examination of endometrial biopsies revealed a pronounced cellular immune response only after deposition of sperm but not after sham insemination.•The number of endometrial immune cells did not differ between hysteroscopic and standard uterine insemination. |
| doi_str_mv | 10.1016/j.jevs.2020.103023 |
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•Effects of standard uterine body and hysteroscopic insemination on endometrial health were investigated by cytology, histology, immunohistochemistry, and microbiology.•Histological and immunohistochemical examination of endometrial biopsies revealed a pronounced cellular immune response only after deposition of sperm but not after sham insemination.•The number of endometrial immune cells did not differ between hysteroscopic and standard uterine insemination.</description><identifier>ISSN: 0737-0806</identifier><identifier>EISSN: 1542-7412</identifier><identifier>DOI: 10.1016/j.jevs.2020.103023</identifier><identifier>PMID: 32534786</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Endometrial biopsy ; Endometrium ; Female ; Horses ; Hysteroscopic insemination ; Immunohistochemistry ; Insemination technique ; Insemination, Artificial - veterinary ; Male ; Ovulation ; Spermatozoa ; Uterus</subject><ispartof>Journal of equine veterinary science, 2020-07, Vol.90, p.103023-103023, Article 103023</ispartof><rights>2020 Elsevier Inc.</rights><rights>Copyright © 2020 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-c13c783cde1bd4d891a06336a06668fc1216b5644eed9ae539dabb28011227663</citedby><cites>FETCH-LOGICAL-c356t-c13c783cde1bd4d891a06336a06668fc1216b5644eed9ae539dabb28011227663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jevs.2020.103023$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32534786$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Köhne, Martin</creatorcontrib><creatorcontrib>Mönnig, Franziska</creatorcontrib><creatorcontrib>Papin, Johanna</creatorcontrib><creatorcontrib>Schöniger, Sandra</creatorcontrib><creatorcontrib>Tönissen, Anna</creatorcontrib><creatorcontrib>Rohn, Karl</creatorcontrib><creatorcontrib>Martinsson, Gunilla</creatorcontrib><creatorcontrib>Schoon, Heinz-Adolf</creatorcontrib><creatorcontrib>Sieme, Harald</creatorcontrib><title>Effects of Hysteroscopic and Uterine Body Insemination on the Presence of Selected Immune Cells in the Equine Endometrium</title><title>Journal of equine veterinary science</title><addtitle>J Equine Vet Sci</addtitle><description>The effects of standard uterine body and hysteroscopic insemination on endometrial health were investigated. For this purpose, 33 mares were assigned to five different protocols: control (no insemination; n = 7), sham AI (sham uterine body insemination; n = 6), sham HysAI (sham hysteroscopic insemination; n = 7), standard AI (standard uterine body insemination, 300 × 106 progressively motile sperms (PMS); n = 7) and HysAI (hysteroscopic insemination, 100 × 106 PMS; n = 6). Sampling included uterine swabbing for microbiological examination, cytology for determination of polymorphonuclear neutrophils (PMNs) in the uterus, and endometrial biopsy collection for histology and characterization of endometrial immune cells on day 18 after ovulation (B1) as well as 8–10 hours (B2, day 20) and 72 hours after insemination (B3, day 23). Microbial contamination increased throughout the experiment in the sham insemination groups. Significant effects (P < .05) over time were detected for PMNs (cytology: sham HysAI, standard AI, and HysAI; histology: standard AI and HysAI), macrophages (immunohistochemistry: standard AI and HysAI) and T cells (immunohistochemistry: standard AI), showing an increase at B2 and a subsequent decrease toward baseline levels at B3. At B2, significant differences (P < .05) existed for PMNs (mean ± SEM) between control (1.3 ± 1.9%) and sham AI (2.2 ± 2.7%) versus standard AI (12.2 ± 4.7%) and for macrophages between control (4.1 ± 3.5%) and sham AI (2.5 ± 1.3%) versus standard AI (25.4 ± 15.8%). Thus, the cellular immune response of the endometrium depends on sperm deposition in the uterus and does not differ between hysteroscopic and standard uterine body insemination.
•Effects of standard uterine body and hysteroscopic insemination on endometrial health were investigated by cytology, histology, immunohistochemistry, and microbiology.•Histological and immunohistochemical examination of endometrial biopsies revealed a pronounced cellular immune response only after deposition of sperm but not after sham insemination.•The number of endometrial immune cells did not differ between hysteroscopic and standard uterine insemination.</description><subject>Animals</subject><subject>Endometrial biopsy</subject><subject>Endometrium</subject><subject>Female</subject><subject>Horses</subject><subject>Hysteroscopic insemination</subject><subject>Immunohistochemistry</subject><subject>Insemination technique</subject><subject>Insemination, Artificial - veterinary</subject><subject>Male</subject><subject>Ovulation</subject><subject>Spermatozoa</subject><subject>Uterus</subject><issn>0737-0806</issn><issn>1542-7412</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LwzAYh4Mobk6_gAfJ0Utn_jXtwIuOqYOBgu4c2uQtZqzNlrSDfXtTOj0KISHh93t48yB0S8mUEiofNtMNHMKUEdY_cML4GRrTVLAkE5SdozHJeJaQnMgRugphQwhLqeCXaMRZykWWyzE6LqoKdBuwq_DbMbTgXdBuZzUuGoPX8W4bwM_OHPGyCVDbpmita3Bc7TfgDw8BGg19_RO2kQQGL-u6i6U5bLcB2yG42Hc9aNEYV0PrbVdfo4uq2Aa4OZ0TtH5ZfM3fktX763L-tEo0T2WbaMp1lnNtgJZGmHxGCyI5l3GXMq80ZVSWqRQCwMwKSPnMFGXJckIpY5mUfILuB-7Ou30HoVW1DTrOVjTguqCYoIJQTqLBCWJDVEcLwUOldt7WhT8qSlSvXG1Ur1z1ytWgPJbuTvyurMH8VX4dx8DjEID4y4MFr4K2vTRjfRSmjLP_8X8ARD-Sdw</recordid><startdate>202007</startdate><enddate>202007</enddate><creator>Köhne, Martin</creator><creator>Mönnig, Franziska</creator><creator>Papin, Johanna</creator><creator>Schöniger, Sandra</creator><creator>Tönissen, Anna</creator><creator>Rohn, Karl</creator><creator>Martinsson, Gunilla</creator><creator>Schoon, Heinz-Adolf</creator><creator>Sieme, Harald</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202007</creationdate><title>Effects of Hysteroscopic and Uterine Body Insemination on the Presence of Selected Immune Cells in the Equine Endometrium</title><author>Köhne, Martin ; Mönnig, Franziska ; Papin, Johanna ; Schöniger, Sandra ; Tönissen, Anna ; Rohn, Karl ; Martinsson, Gunilla ; Schoon, Heinz-Adolf ; Sieme, Harald</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-c13c783cde1bd4d891a06336a06668fc1216b5644eed9ae539dabb28011227663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Endometrial biopsy</topic><topic>Endometrium</topic><topic>Female</topic><topic>Horses</topic><topic>Hysteroscopic insemination</topic><topic>Immunohistochemistry</topic><topic>Insemination technique</topic><topic>Insemination, Artificial - veterinary</topic><topic>Male</topic><topic>Ovulation</topic><topic>Spermatozoa</topic><topic>Uterus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Köhne, Martin</creatorcontrib><creatorcontrib>Mönnig, Franziska</creatorcontrib><creatorcontrib>Papin, Johanna</creatorcontrib><creatorcontrib>Schöniger, Sandra</creatorcontrib><creatorcontrib>Tönissen, Anna</creatorcontrib><creatorcontrib>Rohn, Karl</creatorcontrib><creatorcontrib>Martinsson, Gunilla</creatorcontrib><creatorcontrib>Schoon, Heinz-Adolf</creatorcontrib><creatorcontrib>Sieme, Harald</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of equine veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Köhne, Martin</au><au>Mönnig, Franziska</au><au>Papin, Johanna</au><au>Schöniger, Sandra</au><au>Tönissen, Anna</au><au>Rohn, Karl</au><au>Martinsson, Gunilla</au><au>Schoon, Heinz-Adolf</au><au>Sieme, Harald</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Hysteroscopic and Uterine Body Insemination on the Presence of Selected Immune Cells in the Equine Endometrium</atitle><jtitle>Journal of equine veterinary science</jtitle><addtitle>J Equine Vet Sci</addtitle><date>2020-07</date><risdate>2020</risdate><volume>90</volume><spage>103023</spage><epage>103023</epage><pages>103023-103023</pages><artnum>103023</artnum><issn>0737-0806</issn><eissn>1542-7412</eissn><abstract>The effects of standard uterine body and hysteroscopic insemination on endometrial health were investigated. For this purpose, 33 mares were assigned to five different protocols: control (no insemination; n = 7), sham AI (sham uterine body insemination; n = 6), sham HysAI (sham hysteroscopic insemination; n = 7), standard AI (standard uterine body insemination, 300 × 106 progressively motile sperms (PMS); n = 7) and HysAI (hysteroscopic insemination, 100 × 106 PMS; n = 6). Sampling included uterine swabbing for microbiological examination, cytology for determination of polymorphonuclear neutrophils (PMNs) in the uterus, and endometrial biopsy collection for histology and characterization of endometrial immune cells on day 18 after ovulation (B1) as well as 8–10 hours (B2, day 20) and 72 hours after insemination (B3, day 23). Microbial contamination increased throughout the experiment in the sham insemination groups. Significant effects (P < .05) over time were detected for PMNs (cytology: sham HysAI, standard AI, and HysAI; histology: standard AI and HysAI), macrophages (immunohistochemistry: standard AI and HysAI) and T cells (immunohistochemistry: standard AI), showing an increase at B2 and a subsequent decrease toward baseline levels at B3. At B2, significant differences (P < .05) existed for PMNs (mean ± SEM) between control (1.3 ± 1.9%) and sham AI (2.2 ± 2.7%) versus standard AI (12.2 ± 4.7%) and for macrophages between control (4.1 ± 3.5%) and sham AI (2.5 ± 1.3%) versus standard AI (25.4 ± 15.8%). Thus, the cellular immune response of the endometrium depends on sperm deposition in the uterus and does not differ between hysteroscopic and standard uterine body insemination.
•Effects of standard uterine body and hysteroscopic insemination on endometrial health were investigated by cytology, histology, immunohistochemistry, and microbiology.•Histological and immunohistochemical examination of endometrial biopsies revealed a pronounced cellular immune response only after deposition of sperm but not after sham insemination.•The number of endometrial immune cells did not differ between hysteroscopic and standard uterine insemination.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>32534786</pmid><doi>10.1016/j.jevs.2020.103023</doi><tpages>1</tpages></addata></record> |
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| subjects | Animals Endometrial biopsy Endometrium Female Horses Hysteroscopic insemination Immunohistochemistry Insemination technique Insemination, Artificial - veterinary Male Ovulation Spermatozoa Uterus |
| title | Effects of Hysteroscopic and Uterine Body Insemination on the Presence of Selected Immune Cells in the Equine Endometrium |
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