Towards On-site Determination of Secretory IgA in Artificial Saliva with Gold-Linked Electrochemical Immunoassay (GLEIA) Using Portable Potentiostat and Disposable Printed Electrode
Mental stress is closely connected with our physical and mental wellness. Therefore, stress measurement can contribute to assess our lifestyle and increase our quality of life. In this paper, we detect the secretory immunoglobulin A (sIgA), which is the candidate of salivary stress markers, with ori...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2021-05, Vol.193 (5), p.1311-1320 |
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| creator | Osaki, Shuto Wakida, Shin-ichi Saito, Masato Tamiya, Eiichi |
| description | Mental stress is closely connected with our physical and mental wellness. Therefore, stress measurement can contribute to assess our lifestyle and increase our quality of life. In this paper, we detect the secretory immunoglobulin A (sIgA), which is the candidate of salivary stress markers, with original electrochemical immunoassay: gold-linked electrochemical immunoassay (GLEIA). This biosensor is based on a sandwich-type immunosensor and adopts the electrochemical method to detect the reduction peak from Au nanoparticles linked to the secondary antibody. GLEIA is convenient and cost-effective that only requires a low sample volume (10 μL). In addition, the GLEIA show high sensitivity and selectivity. We obtained the linear response to relate the concentration of sIgA (10–300 ng/mL) in D-PBS buffer with the artificial saliva which includes salivary inorganic salt and typically glycoprotein (mucin). Furthermore, we obtained acceptable selectivity in the various solution with salivary proteins such as α-amylase, human serum albumin, immunoglobulin G (IgG), lysozyme, and mucin. In the future, we try to detect the sIgA in real saliva for on-site stress measurement using GLEIA and to integrate the various immunosensors for stress markers in saliva. |
| doi_str_mv | 10.1007/s12010-020-03332-8 |
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Therefore, stress measurement can contribute to assess our lifestyle and increase our quality of life. In this paper, we detect the secretory immunoglobulin A (sIgA), which is the candidate of salivary stress markers, with original electrochemical immunoassay: gold-linked electrochemical immunoassay (GLEIA). This biosensor is based on a sandwich-type immunosensor and adopts the electrochemical method to detect the reduction peak from Au nanoparticles linked to the secondary antibody. GLEIA is convenient and cost-effective that only requires a low sample volume (10 μL). In addition, the GLEIA show high sensitivity and selectivity. We obtained the linear response to relate the concentration of sIgA (10–300 ng/mL) in D-PBS buffer with the artificial saliva which includes salivary inorganic salt and typically glycoprotein (mucin). Furthermore, we obtained acceptable selectivity in the various solution with salivary proteins such as α-amylase, human serum albumin, immunoglobulin G (IgG), lysozyme, and mucin. In the future, we try to detect the sIgA in real saliva for on-site stress measurement using GLEIA and to integrate the various immunosensors for stress markers in saliva.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-020-03332-8</identifier><identifier>PMID: 32535815</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biochemistry ; Biosensors ; Biotechnology ; Chemistry ; Chemistry and Materials Science ; Electrochemistry ; Glycoproteins ; Gold ; Human serum albumin ; IgG antibody ; Immunoassay ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulins ; Immunosensors ; Inorganic salts ; Lysozyme ; Markers ; Mucin ; Nanoparticles ; Onsite ; Psychological stress ; Quality of life ; Saliva ; Selectivity ; Serum albumin ; α-Amylase</subject><ispartof>Applied biochemistry and biotechnology, 2021-05, Vol.193 (5), p.1311-1320</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-73fa060d2ee034c64186b5cc14f713bce7aca6440dc3e4e06aa2313202c4ba433</citedby><cites>FETCH-LOGICAL-c412t-73fa060d2ee034c64186b5cc14f713bce7aca6440dc3e4e06aa2313202c4ba433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-020-03332-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-020-03332-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32535815$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Osaki, Shuto</creatorcontrib><creatorcontrib>Wakida, Shin-ichi</creatorcontrib><creatorcontrib>Saito, Masato</creatorcontrib><creatorcontrib>Tamiya, Eiichi</creatorcontrib><title>Towards On-site Determination of Secretory IgA in Artificial Saliva with Gold-Linked Electrochemical Immunoassay (GLEIA) Using Portable Potentiostat and Disposable Printed Electrode</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Mental stress is closely connected with our physical and mental wellness. Therefore, stress measurement can contribute to assess our lifestyle and increase our quality of life. In this paper, we detect the secretory immunoglobulin A (sIgA), which is the candidate of salivary stress markers, with original electrochemical immunoassay: gold-linked electrochemical immunoassay (GLEIA). This biosensor is based on a sandwich-type immunosensor and adopts the electrochemical method to detect the reduction peak from Au nanoparticles linked to the secondary antibody. GLEIA is convenient and cost-effective that only requires a low sample volume (10 μL). In addition, the GLEIA show high sensitivity and selectivity. We obtained the linear response to relate the concentration of sIgA (10–300 ng/mL) in D-PBS buffer with the artificial saliva which includes salivary inorganic salt and typically glycoprotein (mucin). Furthermore, we obtained acceptable selectivity in the various solution with salivary proteins such as α-amylase, human serum albumin, immunoglobulin G (IgG), lysozyme, and mucin. In the future, we try to detect the sIgA in real saliva for on-site stress measurement using GLEIA and to integrate the various immunosensors for stress markers in saliva.</description><subject>Biochemistry</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Electrochemistry</subject><subject>Glycoproteins</subject><subject>Gold</subject><subject>Human serum albumin</subject><subject>IgG antibody</subject><subject>Immunoassay</subject><subject>Immunoglobulin A</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulins</subject><subject>Immunosensors</subject><subject>Inorganic salts</subject><subject>Lysozyme</subject><subject>Markers</subject><subject>Mucin</subject><subject>Nanoparticles</subject><subject>Onsite</subject><subject>Psychological stress</subject><subject>Quality of life</subject><subject>Saliva</subject><subject>Selectivity</subject><subject>Serum albumin</subject><subject>α-Amylase</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc9uEzEQhy0EoqHwAhyQJS7lsOCxvX9yjNoQIkUqUtuz5fXOpi67drC9rfJgvB-GLVTiwMGyNfPNbyx9hLwF9hEYqz9F4AxYwXg-QgheNM_IAspymUtLeE4WjNei4LxZnpBXMd4xBrwp65fkRPBSlA2UC_Lj2j_o0EV66YpoE9ILTBhG63Sy3lHf0ys0AZMPR7rdr6h1dBWS7a2xeqBXerD3mj7YdEs3fuiKnXXfsKPrAU0K3tziaE3mtuM4Oa9j1Ed6ttmtt6sP9CZat6dffUi6HTA_Erq8MyadqHYdvbDx4OPcC9alp9gOX5MXvR4ivnm8T8nN5_X1-Zdid7nZnq92hZHAU1GLXrOKdRyRCWkqCU3VlsaA7GsQrcFaG11JyTojUCKrtOYCBGfcyFZLIU7J2Zx7CP77hDGp0UaDw6Ad-ikqLkGyrGJZZfT9P-idn4LLv1O8hGVZQwOQKT5TJvgYA_bqEOyow1EBU7-kqlmqylLVb6mqyUPvHqOndsTu78gfixkQMxBzy-0xPO3-T-xPhdauVQ</recordid><startdate>20210501</startdate><enddate>20210501</enddate><creator>Osaki, Shuto</creator><creator>Wakida, Shin-ichi</creator><creator>Saito, Masato</creator><creator>Tamiya, Eiichi</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>20210501</creationdate><title>Towards On-site Determination of Secretory IgA in Artificial Saliva with Gold-Linked Electrochemical Immunoassay (GLEIA) Using Portable Potentiostat and Disposable Printed Electrode</title><author>Osaki, Shuto ; Wakida, Shin-ichi ; Saito, Masato ; Tamiya, Eiichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-73fa060d2ee034c64186b5cc14f713bce7aca6440dc3e4e06aa2313202c4ba433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biochemistry</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Electrochemistry</topic><topic>Glycoproteins</topic><topic>Gold</topic><topic>Human serum albumin</topic><topic>IgG antibody</topic><topic>Immunoassay</topic><topic>Immunoglobulin A</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulins</topic><topic>Immunosensors</topic><topic>Inorganic salts</topic><topic>Lysozyme</topic><topic>Markers</topic><topic>Mucin</topic><topic>Nanoparticles</topic><topic>Onsite</topic><topic>Psychological stress</topic><topic>Quality of life</topic><topic>Saliva</topic><topic>Selectivity</topic><topic>Serum albumin</topic><topic>α-Amylase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Osaki, Shuto</creatorcontrib><creatorcontrib>Wakida, Shin-ichi</creatorcontrib><creatorcontrib>Saito, Masato</creatorcontrib><creatorcontrib>Tamiya, Eiichi</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Osaki, Shuto</au><au>Wakida, Shin-ichi</au><au>Saito, Masato</au><au>Tamiya, Eiichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Towards On-site Determination of Secretory IgA in Artificial Saliva with Gold-Linked Electrochemical Immunoassay (GLEIA) Using Portable Potentiostat and Disposable Printed Electrode</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2021-05-01</date><risdate>2021</risdate><volume>193</volume><issue>5</issue><spage>1311</spage><epage>1320</epage><pages>1311-1320</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>Mental stress is closely connected with our physical and mental wellness. Therefore, stress measurement can contribute to assess our lifestyle and increase our quality of life. In this paper, we detect the secretory immunoglobulin A (sIgA), which is the candidate of salivary stress markers, with original electrochemical immunoassay: gold-linked electrochemical immunoassay (GLEIA). This biosensor is based on a sandwich-type immunosensor and adopts the electrochemical method to detect the reduction peak from Au nanoparticles linked to the secondary antibody. GLEIA is convenient and cost-effective that only requires a low sample volume (10 μL). In addition, the GLEIA show high sensitivity and selectivity. We obtained the linear response to relate the concentration of sIgA (10–300 ng/mL) in D-PBS buffer with the artificial saliva which includes salivary inorganic salt and typically glycoprotein (mucin). Furthermore, we obtained acceptable selectivity in the various solution with salivary proteins such as α-amylase, human serum albumin, immunoglobulin G (IgG), lysozyme, and mucin. In the future, we try to detect the sIgA in real saliva for on-site stress measurement using GLEIA and to integrate the various immunosensors for stress markers in saliva.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>32535815</pmid><doi>10.1007/s12010-020-03332-8</doi><tpages>10</tpages></addata></record> |
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| subjects | Biochemistry Biosensors Biotechnology Chemistry Chemistry and Materials Science Electrochemistry Glycoproteins Gold Human serum albumin IgG antibody Immunoassay Immunoglobulin A Immunoglobulin G Immunoglobulins Immunosensors Inorganic salts Lysozyme Markers Mucin Nanoparticles Onsite Psychological stress Quality of life Saliva Selectivity Serum albumin α-Amylase |
| title | Towards On-site Determination of Secretory IgA in Artificial Saliva with Gold-Linked Electrochemical Immunoassay (GLEIA) Using Portable Potentiostat and Disposable Printed Electrode |
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