BODIPY-based Fluorescent Probe for the Detection of Cysteine in Living Cells
Cysteine (Cys), as one of the important amino acids, plays a vital role in various physiological and pathological processes. Hence, it is meaningful to develop a convenient and sensitive detection method. Herein, a novel BODIPY-based fluorescent probe (BDP-DM) was developed, which had a higher selec...
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| Veröffentlicht in: | Analytical Sciences 2020/11/10, Vol.36(11), pp.1317-1322 |
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| container_title | Analytical Sciences |
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| creator | WANG, Nannan JI, Xin WANG, Han WANG, Xianhui TAO, Yanfang ZHAO, Weili ZHANG, Jian |
| description | Cysteine (Cys), as one of the important amino acids, plays a vital role in various physiological and pathological processes. Hence, it is meaningful to develop a convenient and sensitive detection method. Herein, a novel BODIPY-based fluorescent probe (BDP-DM) was developed, which had a higher selectivity for Cys than other amino acids, including homocysteine (Hcy) and glutathione (GSH). Ultimately, we concluded that the BDP-DM probe could be used to successfully detected intracellular Cys in living HeLa cells. |
| doi_str_mv | 10.2116/analsci.20P134 |
| format | Article |
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Hence, it is meaningful to develop a convenient and sensitive detection method. Herein, a novel BODIPY-based fluorescent probe (BDP-DM) was developed, which had a higher selectivity for Cys than other amino acids, including homocysteine (Hcy) and glutathione (GSH). Ultimately, we concluded that the BDP-DM probe could be used to successfully detected intracellular Cys in living HeLa cells.</description><identifier>ISSN: 0910-6340</identifier><identifier>EISSN: 1348-2246</identifier><identifier>DOI: 10.2116/analsci.20P134</identifier><identifier>PMID: 32536622</identifier><language>eng</language><publisher>Singapore: The Japan Society for Analytical Chemistry</publisher><subject>Amino acids ; Analytical Chemistry ; bioimaging ; BODIPY ; Boron Compounds - chemistry ; Cell Survival ; Chemistry ; Cysteine ; Cysteine - analysis ; Cysteine - chemistry ; Fluorescent Dyes - chemistry ; Fluorescent indicators ; fluorescent probe ; Glutathione ; HeLa Cells ; Homocysteine ; Humans ; Limit of Detection ; Selectivity</subject><ispartof>Analytical Sciences, 2020/11/10, Vol.36(11), pp.1317-1322</ispartof><rights>2020 by The Japan Society for Analytical Chemistry</rights><rights>The Japan Society for Analytical Chemistry 2020</rights><rights>Copyright Japan Science and Technology Agency 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c683t-3f1c7ebea7a675c229e6ba6499fba572e0e02f87314b007dfa95678302a3647a3</citedby><cites>FETCH-LOGICAL-c683t-3f1c7ebea7a675c229e6ba6499fba572e0e02f87314b007dfa95678302a3647a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.2116/analsci.20P134$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.2116/analsci.20P134$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,1881,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32536622$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WANG, Nannan</creatorcontrib><creatorcontrib>JI, Xin</creatorcontrib><creatorcontrib>WANG, Han</creatorcontrib><creatorcontrib>WANG, Xianhui</creatorcontrib><creatorcontrib>TAO, Yanfang</creatorcontrib><creatorcontrib>ZHAO, Weili</creatorcontrib><creatorcontrib>ZHANG, Jian</creatorcontrib><title>BODIPY-based Fluorescent Probe for the Detection of Cysteine in Living Cells</title><title>Analytical Sciences</title><addtitle>ANAL. 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Ultimately, we concluded that the BDP-DM probe could be used to successfully detected intracellular Cys in living HeLa cells.</description><subject>Amino acids</subject><subject>Analytical Chemistry</subject><subject>bioimaging</subject><subject>BODIPY</subject><subject>Boron Compounds - chemistry</subject><subject>Cell Survival</subject><subject>Chemistry</subject><subject>Cysteine</subject><subject>Cysteine - analysis</subject><subject>Cysteine - chemistry</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent indicators</subject><subject>fluorescent probe</subject><subject>Glutathione</subject><subject>HeLa Cells</subject><subject>Homocysteine</subject><subject>Humans</subject><subject>Limit of Detection</subject><subject>Selectivity</subject><issn>0910-6340</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEFrGzEQRkVpadw01x6LoJde1pFGa-3q2DpJEzDEh_aQk9DKs47MWkolbSD_vjLrOlDoZXSY930aHiGfOJsD5_LSeDMk6-bA1lzUb8iszLYCqOVbMmOKs0qKmp2RDyntGOPQArwnZwIWQkqAGVl9v7-6Wz9UnUm4oTfDGCImiz7TdQwd0j5Emh-RXmFGm13wNPR0-ZIyOo_Uebpyz85v6RKHIX0k7_pyDl4c33Py6-b65_K2Wt3_uFt-W1VWtiJXoue2wQ5NY2SzsAAKZWdkrVTfmUUDyJBB3zaC1x1jzaY3aiGbVjAwQtaNEefk69T7FMPvEVPWe1eOHgbjMYxJQ82FUgpaVtAv_6C7MMaDtEJJySRTrC3UfKJsDClF7PVTdHsTXzRn-uBZHz3ryXMJfD7Wjt0eNyf8r9gCXE5AKiu_xfj6738rr6fELmWzxVOlidnZAU-4kJrzw5xyp719NFGjF38AP36gzg</recordid><startdate>20201110</startdate><enddate>20201110</enddate><creator>WANG, Nannan</creator><creator>JI, Xin</creator><creator>WANG, Han</creator><creator>WANG, Xianhui</creator><creator>TAO, Yanfang</creator><creator>ZHAO, Weili</creator><creator>ZHANG, Jian</creator><general>The Japan Society for Analytical Chemistry</general><general>Springer Nature Singapore</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20201110</creationdate><title>BODIPY-based Fluorescent Probe for the Detection of Cysteine in Living Cells</title><author>WANG, Nannan ; 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| subjects | Amino acids Analytical Chemistry bioimaging BODIPY Boron Compounds - chemistry Cell Survival Chemistry Cysteine Cysteine - analysis Cysteine - chemistry Fluorescent Dyes - chemistry Fluorescent indicators fluorescent probe Glutathione HeLa Cells Homocysteine Humans Limit of Detection Selectivity |
| title | BODIPY-based Fluorescent Probe for the Detection of Cysteine in Living Cells |
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