Pullulan microneedle patches for the efficient transdermal administration of insulin envisioning diabetes treatment
[Display omitted] •Dissolvable pullulan microneedles were successfully prepared by solvent casting.•Pullulan-insulin microneedles successfully penetrate through the skin.•Storage at 4, 20 and 40 °C maintained the secondary structure of insulin.•Pullulan microneedles deliver 87% of insulin, 120 min a...
Gespeichert in:
| Veröffentlicht in: | Carbohydrate polymers 2020-08, Vol.241, p.116314-116314, Article 116314 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 116314 |
|---|---|
| container_issue | |
| container_start_page | 116314 |
| container_title | Carbohydrate polymers |
| container_volume | 241 |
| creator | Fonseca, Daniela F.S. Costa, Paulo C. Almeida, Isabel F. Dias-Pereira, Patrícia Correia-Sá, Inês Bastos, Verónica Oliveira, Helena Duarte-Araújo, Margarida Morato, Manuela Vilela, Carla Silvestre, Armando J.D. Freire, Carmen S.R. |
| description | [Display omitted]
•Dissolvable pullulan microneedles were successfully prepared by solvent casting.•Pullulan-insulin microneedles successfully penetrate through the skin.•Storage at 4, 20 and 40 °C maintained the secondary structure of insulin.•Pullulan microneedles deliver 87% of insulin, 120 min after insertion.•Pullulan-insulin microneedles are non-cytotoxic towards human keratinocyte cells.
The present study reports the fabrication of dissolvable microneedle (MN) patches using pullulan (PL), a water-soluble polysaccharide with excellent film-forming ability, for the transdermal administration of insulin, envisioning the non-invasive treatment of diabetes. PL MNs patches were successfully prepared by micromoulding and revealed good thermal stability (Tdmax = 294 °C) and mechanical properties (>0.15 N needle−1), penetrating skin up to 381 μm depth, as revealed by in vitro skin tests. After application into human abdominal skin in vitro, the MNs dissolved within 2 h releasing up to 87% of insulin. When stored at 4, 20 and 40 °C for 4 weeks, insulin was able to retain its secondary structure, as shown by circular dichroism spectropolarimetry. The prepared PL MNs were non-cytotoxic towards human keratinocytes, being suitable for skin application. These findings suggest that PL MNs have potential to deliver insulin transdermally, thus avoiding its subcutaneous administration. |
| doi_str_mv | 10.1016/j.carbpol.2020.116314 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2410731465</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0144861720304884</els_id><sourcerecordid>2410731465</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-849d917da8d1391f1528ef43d3164a7cbcd2acc2139c847e21df9abcc19bdb183</originalsourceid><addsrcrecordid>eNqFkMtKBDEQRYMoOj4-QcnSTY-p7kw_ViLiCwZ0oeuQTiqaIZ0ek7Tg35thRrfWpuDWrVvUIeQc2BwY1FeruZKhX49uXrIya1BXwPfIDNqmK6DifJ_MGHBetDU0R-Q4xhXLVQM7JEdVuWANdDAj8WVybnLS08GqMHpE7ZCuZVIfGKkZA00fSNEYqyz6RFOQPmoMg3RU6sF6G7OU7OjpaKj1cXLWU_RfNmbN-neqrewx5bAUUKYhh5ySAyNdxLNdPyFv93evt4_F8vnh6fZmWaiqg1S0vNMdNFq2GrJgYFG2aHilK6i5bFSvdCmVKvNQtbzBErTpZK8UdL3uoa1OyOU2dx3GzwljEoONCl3-FscpipIDazK1epGti601M4gxoBHrYAcZvgUwseEtVmLHW2x4iy3vvHexOzH1A-q_rV_A2XC9NWB-9MtiEHEDUqG2AVUSerT_nPgBKRaXLQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2410731465</pqid></control><display><type>article</type><title>Pullulan microneedle patches for the efficient transdermal administration of insulin envisioning diabetes treatment</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Fonseca, Daniela F.S. ; Costa, Paulo C. ; Almeida, Isabel F. ; Dias-Pereira, Patrícia ; Correia-Sá, Inês ; Bastos, Verónica ; Oliveira, Helena ; Duarte-Araújo, Margarida ; Morato, Manuela ; Vilela, Carla ; Silvestre, Armando J.D. ; Freire, Carmen S.R.</creator><creatorcontrib>Fonseca, Daniela F.S. ; Costa, Paulo C. ; Almeida, Isabel F. ; Dias-Pereira, Patrícia ; Correia-Sá, Inês ; Bastos, Verónica ; Oliveira, Helena ; Duarte-Araújo, Margarida ; Morato, Manuela ; Vilela, Carla ; Silvestre, Armando J.D. ; Freire, Carmen S.R.</creatorcontrib><description>[Display omitted]
•Dissolvable pullulan microneedles were successfully prepared by solvent casting.•Pullulan-insulin microneedles successfully penetrate through the skin.•Storage at 4, 20 and 40 °C maintained the secondary structure of insulin.•Pullulan microneedles deliver 87% of insulin, 120 min after insertion.•Pullulan-insulin microneedles are non-cytotoxic towards human keratinocyte cells.
The present study reports the fabrication of dissolvable microneedle (MN) patches using pullulan (PL), a water-soluble polysaccharide with excellent film-forming ability, for the transdermal administration of insulin, envisioning the non-invasive treatment of diabetes. PL MNs patches were successfully prepared by micromoulding and revealed good thermal stability (Tdmax = 294 °C) and mechanical properties (>0.15 N needle−1), penetrating skin up to 381 μm depth, as revealed by in vitro skin tests. After application into human abdominal skin in vitro, the MNs dissolved within 2 h releasing up to 87% of insulin. When stored at 4, 20 and 40 °C for 4 weeks, insulin was able to retain its secondary structure, as shown by circular dichroism spectropolarimetry. The prepared PL MNs were non-cytotoxic towards human keratinocytes, being suitable for skin application. These findings suggest that PL MNs have potential to deliver insulin transdermally, thus avoiding its subcutaneous administration.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2020.116314</identifier><identifier>PMID: 32507191</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Administration, Cutaneous ; Diabetes Mellitus - drug therapy ; Drug Delivery Systems - instrumentation ; Glucans - chemistry ; HaCaT Cells ; Humans ; Hypoglycemic Agents - administration & dosage ; Insulin ; Insulin - administration & dosage ; Microneedles ; Needles ; Polysaccharides ; Pullulan ; Transdermal drug delivery ; Transdermal Patch</subject><ispartof>Carbohydrate polymers, 2020-08, Vol.241, p.116314-116314, Article 116314</ispartof><rights>2020 Elsevier Ltd</rights><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-849d917da8d1391f1528ef43d3164a7cbcd2acc2139c847e21df9abcc19bdb183</citedby><cites>FETCH-LOGICAL-c391t-849d917da8d1391f1528ef43d3164a7cbcd2acc2139c847e21df9abcc19bdb183</cites><orcidid>0000-0003-4727-727X ; 0000-0002-1152-3398 ; 0000-0002-6320-4663 ; 0000-0001-5403-8416 ; 0000-0002-1498-048X ; 0000-0002-9212-2704</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0144861720304884$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32507191$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fonseca, Daniela F.S.</creatorcontrib><creatorcontrib>Costa, Paulo C.</creatorcontrib><creatorcontrib>Almeida, Isabel F.</creatorcontrib><creatorcontrib>Dias-Pereira, Patrícia</creatorcontrib><creatorcontrib>Correia-Sá, Inês</creatorcontrib><creatorcontrib>Bastos, Verónica</creatorcontrib><creatorcontrib>Oliveira, Helena</creatorcontrib><creatorcontrib>Duarte-Araújo, Margarida</creatorcontrib><creatorcontrib>Morato, Manuela</creatorcontrib><creatorcontrib>Vilela, Carla</creatorcontrib><creatorcontrib>Silvestre, Armando J.D.</creatorcontrib><creatorcontrib>Freire, Carmen S.R.</creatorcontrib><title>Pullulan microneedle patches for the efficient transdermal administration of insulin envisioning diabetes treatment</title><title>Carbohydrate polymers</title><addtitle>Carbohydr Polym</addtitle><description>[Display omitted]
•Dissolvable pullulan microneedles were successfully prepared by solvent casting.•Pullulan-insulin microneedles successfully penetrate through the skin.•Storage at 4, 20 and 40 °C maintained the secondary structure of insulin.•Pullulan microneedles deliver 87% of insulin, 120 min after insertion.•Pullulan-insulin microneedles are non-cytotoxic towards human keratinocyte cells.
The present study reports the fabrication of dissolvable microneedle (MN) patches using pullulan (PL), a water-soluble polysaccharide with excellent film-forming ability, for the transdermal administration of insulin, envisioning the non-invasive treatment of diabetes. PL MNs patches were successfully prepared by micromoulding and revealed good thermal stability (Tdmax = 294 °C) and mechanical properties (>0.15 N needle−1), penetrating skin up to 381 μm depth, as revealed by in vitro skin tests. After application into human abdominal skin in vitro, the MNs dissolved within 2 h releasing up to 87% of insulin. When stored at 4, 20 and 40 °C for 4 weeks, insulin was able to retain its secondary structure, as shown by circular dichroism spectropolarimetry. The prepared PL MNs were non-cytotoxic towards human keratinocytes, being suitable for skin application. These findings suggest that PL MNs have potential to deliver insulin transdermally, thus avoiding its subcutaneous administration.</description><subject>Administration, Cutaneous</subject><subject>Diabetes Mellitus - drug therapy</subject><subject>Drug Delivery Systems - instrumentation</subject><subject>Glucans - chemistry</subject><subject>HaCaT Cells</subject><subject>Humans</subject><subject>Hypoglycemic Agents - administration & dosage</subject><subject>Insulin</subject><subject>Insulin - administration & dosage</subject><subject>Microneedles</subject><subject>Needles</subject><subject>Polysaccharides</subject><subject>Pullulan</subject><subject>Transdermal drug delivery</subject><subject>Transdermal Patch</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKBDEQRYMoOj4-QcnSTY-p7kw_ViLiCwZ0oeuQTiqaIZ0ek7Tg35thRrfWpuDWrVvUIeQc2BwY1FeruZKhX49uXrIya1BXwPfIDNqmK6DifJ_MGHBetDU0R-Q4xhXLVQM7JEdVuWANdDAj8WVybnLS08GqMHpE7ZCuZVIfGKkZA00fSNEYqyz6RFOQPmoMg3RU6sF6G7OU7OjpaKj1cXLWU_RfNmbN-neqrewx5bAUUKYhh5ySAyNdxLNdPyFv93evt4_F8vnh6fZmWaiqg1S0vNMdNFq2GrJgYFG2aHilK6i5bFSvdCmVKvNQtbzBErTpZK8UdL3uoa1OyOU2dx3GzwljEoONCl3-FscpipIDazK1epGti601M4gxoBHrYAcZvgUwseEtVmLHW2x4iy3vvHexOzH1A-q_rV_A2XC9NWB-9MtiEHEDUqG2AVUSerT_nPgBKRaXLQ</recordid><startdate>20200801</startdate><enddate>20200801</enddate><creator>Fonseca, Daniela F.S.</creator><creator>Costa, Paulo C.</creator><creator>Almeida, Isabel F.</creator><creator>Dias-Pereira, Patrícia</creator><creator>Correia-Sá, Inês</creator><creator>Bastos, Verónica</creator><creator>Oliveira, Helena</creator><creator>Duarte-Araújo, Margarida</creator><creator>Morato, Manuela</creator><creator>Vilela, Carla</creator><creator>Silvestre, Armando J.D.</creator><creator>Freire, Carmen S.R.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4727-727X</orcidid><orcidid>https://orcid.org/0000-0002-1152-3398</orcidid><orcidid>https://orcid.org/0000-0002-6320-4663</orcidid><orcidid>https://orcid.org/0000-0001-5403-8416</orcidid><orcidid>https://orcid.org/0000-0002-1498-048X</orcidid><orcidid>https://orcid.org/0000-0002-9212-2704</orcidid></search><sort><creationdate>20200801</creationdate><title>Pullulan microneedle patches for the efficient transdermal administration of insulin envisioning diabetes treatment</title><author>Fonseca, Daniela F.S. ; Costa, Paulo C. ; Almeida, Isabel F. ; Dias-Pereira, Patrícia ; Correia-Sá, Inês ; Bastos, Verónica ; Oliveira, Helena ; Duarte-Araújo, Margarida ; Morato, Manuela ; Vilela, Carla ; Silvestre, Armando J.D. ; Freire, Carmen S.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-849d917da8d1391f1528ef43d3164a7cbcd2acc2139c847e21df9abcc19bdb183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Administration, Cutaneous</topic><topic>Diabetes Mellitus - drug therapy</topic><topic>Drug Delivery Systems - instrumentation</topic><topic>Glucans - chemistry</topic><topic>HaCaT Cells</topic><topic>Humans</topic><topic>Hypoglycemic Agents - administration & dosage</topic><topic>Insulin</topic><topic>Insulin - administration & dosage</topic><topic>Microneedles</topic><topic>Needles</topic><topic>Polysaccharides</topic><topic>Pullulan</topic><topic>Transdermal drug delivery</topic><topic>Transdermal Patch</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fonseca, Daniela F.S.</creatorcontrib><creatorcontrib>Costa, Paulo C.</creatorcontrib><creatorcontrib>Almeida, Isabel F.</creatorcontrib><creatorcontrib>Dias-Pereira, Patrícia</creatorcontrib><creatorcontrib>Correia-Sá, Inês</creatorcontrib><creatorcontrib>Bastos, Verónica</creatorcontrib><creatorcontrib>Oliveira, Helena</creatorcontrib><creatorcontrib>Duarte-Araújo, Margarida</creatorcontrib><creatorcontrib>Morato, Manuela</creatorcontrib><creatorcontrib>Vilela, Carla</creatorcontrib><creatorcontrib>Silvestre, Armando J.D.</creatorcontrib><creatorcontrib>Freire, Carmen S.R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fonseca, Daniela F.S.</au><au>Costa, Paulo C.</au><au>Almeida, Isabel F.</au><au>Dias-Pereira, Patrícia</au><au>Correia-Sá, Inês</au><au>Bastos, Verónica</au><au>Oliveira, Helena</au><au>Duarte-Araújo, Margarida</au><au>Morato, Manuela</au><au>Vilela, Carla</au><au>Silvestre, Armando J.D.</au><au>Freire, Carmen S.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pullulan microneedle patches for the efficient transdermal administration of insulin envisioning diabetes treatment</atitle><jtitle>Carbohydrate polymers</jtitle><addtitle>Carbohydr Polym</addtitle><date>2020-08-01</date><risdate>2020</risdate><volume>241</volume><spage>116314</spage><epage>116314</epage><pages>116314-116314</pages><artnum>116314</artnum><issn>0144-8617</issn><eissn>1879-1344</eissn><abstract>[Display omitted]
•Dissolvable pullulan microneedles were successfully prepared by solvent casting.•Pullulan-insulin microneedles successfully penetrate through the skin.•Storage at 4, 20 and 40 °C maintained the secondary structure of insulin.•Pullulan microneedles deliver 87% of insulin, 120 min after insertion.•Pullulan-insulin microneedles are non-cytotoxic towards human keratinocyte cells.
The present study reports the fabrication of dissolvable microneedle (MN) patches using pullulan (PL), a water-soluble polysaccharide with excellent film-forming ability, for the transdermal administration of insulin, envisioning the non-invasive treatment of diabetes. PL MNs patches were successfully prepared by micromoulding and revealed good thermal stability (Tdmax = 294 °C) and mechanical properties (>0.15 N needle−1), penetrating skin up to 381 μm depth, as revealed by in vitro skin tests. After application into human abdominal skin in vitro, the MNs dissolved within 2 h releasing up to 87% of insulin. When stored at 4, 20 and 40 °C for 4 weeks, insulin was able to retain its secondary structure, as shown by circular dichroism spectropolarimetry. The prepared PL MNs were non-cytotoxic towards human keratinocytes, being suitable for skin application. These findings suggest that PL MNs have potential to deliver insulin transdermally, thus avoiding its subcutaneous administration.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>32507191</pmid><doi>10.1016/j.carbpol.2020.116314</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-4727-727X</orcidid><orcidid>https://orcid.org/0000-0002-1152-3398</orcidid><orcidid>https://orcid.org/0000-0002-6320-4663</orcidid><orcidid>https://orcid.org/0000-0001-5403-8416</orcidid><orcidid>https://orcid.org/0000-0002-1498-048X</orcidid><orcidid>https://orcid.org/0000-0002-9212-2704</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0144-8617 |
| ispartof | Carbohydrate polymers, 2020-08, Vol.241, p.116314-116314, Article 116314 |
| issn | 0144-8617 1879-1344 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2410731465 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Administration, Cutaneous Diabetes Mellitus - drug therapy Drug Delivery Systems - instrumentation Glucans - chemistry HaCaT Cells Humans Hypoglycemic Agents - administration & dosage Insulin Insulin - administration & dosage Microneedles Needles Polysaccharides Pullulan Transdermal drug delivery Transdermal Patch |
| title | Pullulan microneedle patches for the efficient transdermal administration of insulin envisioning diabetes treatment |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T06%3A28%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pullulan%20microneedle%20patches%20for%20the%20efficient%20transdermal%20administration%20of%20insulin%20envisioning%20diabetes%20treatment&rft.jtitle=Carbohydrate%20polymers&rft.au=Fonseca,%20Daniela%20F.S.&rft.date=2020-08-01&rft.volume=241&rft.spage=116314&rft.epage=116314&rft.pages=116314-116314&rft.artnum=116314&rft.issn=0144-8617&rft.eissn=1879-1344&rft_id=info:doi/10.1016/j.carbpol.2020.116314&rft_dat=%3Cproquest_cross%3E2410731465%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2410731465&rft_id=info:pmid/32507191&rft_els_id=S0144861720304884&rfr_iscdi=true |