Detection of zoonotic human pathogens from Ixodes scapularis in Wisconsin
Extracted nucleic acids were also tested using real-time fluorescent resonance energy transfer (FRET) PCR assays for B. miyamotoi (GlpQ: Forward primer 5'-TCCAGAACATACCTTAGAAGC- 3'; reverse primer 5'-ATCAAATCTTTCACTGAGACTTA-3'; fluorescein-labeled probe 5'-GACAATGTTCCTATTATA...
Gespeichert in:
| Veröffentlicht in: | Journal of vector ecology 2020-06, Vol.45 (1), p.142-144 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 144 |
|---|---|
| container_issue | 1 |
| container_start_page | 142 |
| container_title | Journal of vector ecology |
| container_volume | 45 |
| creator | Stauffer, Maxwell T Mandli, Jordan Pritt, Bobbi S Stauffer, William Sloan, Lynne M Zembsch, Tela Lee, Xia Paskewitz, Susan M |
| description | Extracted nucleic acids were also tested using real-time fluorescent resonance energy transfer (FRET) PCR assays for B. miyamotoi (GlpQ: Forward primer 5'-TCCAGAACATACCTTAGAAGC- 3'; reverse primer 5'-ATCAAATCTTTCACTGAGACTTA-3'; fluorescein-labeled probe 5'-GACAATGTTCCTATTATAATGCACGACCC-fl-3'; 5'-LC640-GAAATTGACACAACCACAAATGTTGCAC-3'), and Powassan virus (NS5: forward primer 5'-ACTAGAATGGCCATGACAGAC-3'; reverse primer 5'-TCATCTCTGGTGCACATCC-3'; fluorescein-labeled probe 5'-CACAAAGGCCCAGGAACCACAGC-fl-3'; red labeled probe 5'-LC640-GGCACCAGIGTGATCATGAGAGCIGT-3'); (WSH site only because ticks were stored in ethanol at WAL). [...]a relatively small number of ticks were analyzed and only nymphs were present in large numbers at the southeastern location (WAL). [...]inconsistent tick storage limited our ability to detect the full spectrum of pathogens. [...]this study demonstrates pathogen prevalence and coinfections in I. scapularis ticks among regions of the upper midwestern United States. |
| doi_str_mv | 10.1111/jvec.12384 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2409645849</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2424471480</sourcerecordid><originalsourceid>FETCH-LOGICAL-b4264-c0cc273dd871b24680e270e928ab0fa2c0fc9808ce55574513330bedc21475223</originalsourceid><addsrcrecordid>eNp90U9PwyAYBnBiNG5OL34AQ-LFmHQCpS09mjl1ZokX_xwbSt86lhZmadX56WVu8-BhXODwy_PCA0KnlAypX1fzD1BDykLB91CfplwECQ35vj8TQQOaUNJDR87NCfFExIeoFzKeMpaEfTS5gRZUq63BtsTf1hrbaoVnXS0NXsh2Zt_AOFw2tsaTL1uAw07JRVfJRjusDX7VTlnjtDlGB6WsHJxs9gF6vh0_je6D6ePdZHQ9DXLOYh4oopSfXBQioTnjsSDAEgIpEzInpWSKlCoVRCiIoijhEQ3DkORQKEZ5EjEWDtDFOnfR2PcOXJvV_gpQVdKA7VzGOEljHgmeenr-j85t1xh_O68Y5wnlgnh1uVaqsc41UGaLRteyWWaUZKuCs1XB2W_BHp9tIru8huKPbhv1gK7Bp65guSMqe3gZj7ahmyfl2n8A7Jr_A-VWkWs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2424471480</pqid></control><display><type>article</type><title>Detection of zoonotic human pathogens from Ixodes scapularis in Wisconsin</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Stauffer, Maxwell T ; Mandli, Jordan ; Pritt, Bobbi S ; Stauffer, William ; Sloan, Lynne M ; Zembsch, Tela ; Lee, Xia ; Paskewitz, Susan M</creator><creatorcontrib>Stauffer, Maxwell T ; Mandli, Jordan ; Pritt, Bobbi S ; Stauffer, William ; Sloan, Lynne M ; Zembsch, Tela ; Lee, Xia ; Paskewitz, Susan M</creatorcontrib><description>Extracted nucleic acids were also tested using real-time fluorescent resonance energy transfer (FRET) PCR assays for B. miyamotoi (GlpQ: Forward primer 5'-TCCAGAACATACCTTAGAAGC- 3'; reverse primer 5'-ATCAAATCTTTCACTGAGACTTA-3'; fluorescein-labeled probe 5'-GACAATGTTCCTATTATAATGCACGACCC-fl-3'; 5'-LC640-GAAATTGACACAACCACAAATGTTGCAC-3'), and Powassan virus (NS5: forward primer 5'-ACTAGAATGGCCATGACAGAC-3'; reverse primer 5'-TCATCTCTGGTGCACATCC-3'; fluorescein-labeled probe 5'-CACAAAGGCCCAGGAACCACAGC-fl-3'; red labeled probe 5'-LC640-GGCACCAGIGTGATCATGAGAGCIGT-3'); (WSH site only because ticks were stored in ethanol at WAL). [...]a relatively small number of ticks were analyzed and only nymphs were present in large numbers at the southeastern location (WAL). [...]inconsistent tick storage limited our ability to detect the full spectrum of pathogens. [...]this study demonstrates pathogen prevalence and coinfections in I. scapularis ticks among regions of the upper midwestern United States.</description><identifier>ISSN: 1081-1710</identifier><identifier>EISSN: 1948-7134</identifier><identifier>DOI: 10.1111/jvec.12384</identifier><identifier>PMID: 32492273</identifier><language>eng</language><publisher>United States: Society for Vector Ecology</publisher><subject>Adults ; Arachnids ; Energy transfer ; Ethanol ; Fluorescein ; Fluorescence ; Fluorescence resonance energy transfer ; Infections ; Nucleic acids ; Pathogens ; SCIENTIFIC NOTES ; Ticks ; Viruses</subject><ispartof>Journal of vector ecology, 2020-06, Vol.45 (1), p.142-144</ispartof><rights>2020 The Society for Vector Ecology</rights><rights>Copyright Society for Vector Ecology Jun 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b4264-c0cc273dd871b24680e270e928ab0fa2c0fc9808ce55574513330bedc21475223</citedby><cites>FETCH-LOGICAL-b4264-c0cc273dd871b24680e270e928ab0fa2c0fc9808ce55574513330bedc21475223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32492273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stauffer, Maxwell T</creatorcontrib><creatorcontrib>Mandli, Jordan</creatorcontrib><creatorcontrib>Pritt, Bobbi S</creatorcontrib><creatorcontrib>Stauffer, William</creatorcontrib><creatorcontrib>Sloan, Lynne M</creatorcontrib><creatorcontrib>Zembsch, Tela</creatorcontrib><creatorcontrib>Lee, Xia</creatorcontrib><creatorcontrib>Paskewitz, Susan M</creatorcontrib><title>Detection of zoonotic human pathogens from Ixodes scapularis in Wisconsin</title><title>Journal of vector ecology</title><addtitle>J Vector Ecol</addtitle><description>Extracted nucleic acids were also tested using real-time fluorescent resonance energy transfer (FRET) PCR assays for B. miyamotoi (GlpQ: Forward primer 5'-TCCAGAACATACCTTAGAAGC- 3'; reverse primer 5'-ATCAAATCTTTCACTGAGACTTA-3'; fluorescein-labeled probe 5'-GACAATGTTCCTATTATAATGCACGACCC-fl-3'; 5'-LC640-GAAATTGACACAACCACAAATGTTGCAC-3'), and Powassan virus (NS5: forward primer 5'-ACTAGAATGGCCATGACAGAC-3'; reverse primer 5'-TCATCTCTGGTGCACATCC-3'; fluorescein-labeled probe 5'-CACAAAGGCCCAGGAACCACAGC-fl-3'; red labeled probe 5'-LC640-GGCACCAGIGTGATCATGAGAGCIGT-3'); (WSH site only because ticks were stored in ethanol at WAL). [...]a relatively small number of ticks were analyzed and only nymphs were present in large numbers at the southeastern location (WAL). [...]inconsistent tick storage limited our ability to detect the full spectrum of pathogens. [...]this study demonstrates pathogen prevalence and coinfections in I. scapularis ticks among regions of the upper midwestern United States.</description><subject>Adults</subject><subject>Arachnids</subject><subject>Energy transfer</subject><subject>Ethanol</subject><subject>Fluorescein</subject><subject>Fluorescence</subject><subject>Fluorescence resonance energy transfer</subject><subject>Infections</subject><subject>Nucleic acids</subject><subject>Pathogens</subject><subject>SCIENTIFIC NOTES</subject><subject>Ticks</subject><subject>Viruses</subject><issn>1081-1710</issn><issn>1948-7134</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp90U9PwyAYBnBiNG5OL34AQ-LFmHQCpS09mjl1ZokX_xwbSt86lhZmadX56WVu8-BhXODwy_PCA0KnlAypX1fzD1BDykLB91CfplwECQ35vj8TQQOaUNJDR87NCfFExIeoFzKeMpaEfTS5gRZUq63BtsTf1hrbaoVnXS0NXsh2Zt_AOFw2tsaTL1uAw07JRVfJRjusDX7VTlnjtDlGB6WsHJxs9gF6vh0_je6D6ePdZHQ9DXLOYh4oopSfXBQioTnjsSDAEgIpEzInpWSKlCoVRCiIoijhEQ3DkORQKEZ5EjEWDtDFOnfR2PcOXJvV_gpQVdKA7VzGOEljHgmeenr-j85t1xh_O68Y5wnlgnh1uVaqsc41UGaLRteyWWaUZKuCs1XB2W_BHp9tIru8huKPbhv1gK7Bp65guSMqe3gZj7ahmyfl2n8A7Jr_A-VWkWs</recordid><startdate>202006</startdate><enddate>202006</enddate><creator>Stauffer, Maxwell T</creator><creator>Mandli, Jordan</creator><creator>Pritt, Bobbi S</creator><creator>Stauffer, William</creator><creator>Sloan, Lynne M</creator><creator>Zembsch, Tela</creator><creator>Lee, Xia</creator><creator>Paskewitz, Susan M</creator><general>Society for Vector Ecology</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PATMY</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope><scope>7X8</scope></search><sort><creationdate>202006</creationdate><title>Detection of zoonotic human pathogens from Ixodes scapularis in Wisconsin</title><author>Stauffer, Maxwell T ; Mandli, Jordan ; Pritt, Bobbi S ; Stauffer, William ; Sloan, Lynne M ; Zembsch, Tela ; Lee, Xia ; Paskewitz, Susan M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b4264-c0cc273dd871b24680e270e928ab0fa2c0fc9808ce55574513330bedc21475223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adults</topic><topic>Arachnids</topic><topic>Energy transfer</topic><topic>Ethanol</topic><topic>Fluorescein</topic><topic>Fluorescence</topic><topic>Fluorescence resonance energy transfer</topic><topic>Infections</topic><topic>Nucleic acids</topic><topic>Pathogens</topic><topic>SCIENTIFIC NOTES</topic><topic>Ticks</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stauffer, Maxwell T</creatorcontrib><creatorcontrib>Mandli, Jordan</creatorcontrib><creatorcontrib>Pritt, Bobbi S</creatorcontrib><creatorcontrib>Stauffer, William</creatorcontrib><creatorcontrib>Sloan, Lynne M</creatorcontrib><creatorcontrib>Zembsch, Tela</creatorcontrib><creatorcontrib>Lee, Xia</creatorcontrib><creatorcontrib>Paskewitz, Susan M</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Environmental Science Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Environmental Science Collection</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of vector ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stauffer, Maxwell T</au><au>Mandli, Jordan</au><au>Pritt, Bobbi S</au><au>Stauffer, William</au><au>Sloan, Lynne M</au><au>Zembsch, Tela</au><au>Lee, Xia</au><au>Paskewitz, Susan M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of zoonotic human pathogens from Ixodes scapularis in Wisconsin</atitle><jtitle>Journal of vector ecology</jtitle><addtitle>J Vector Ecol</addtitle><date>2020-06</date><risdate>2020</risdate><volume>45</volume><issue>1</issue><spage>142</spage><epage>144</epage><pages>142-144</pages><issn>1081-1710</issn><eissn>1948-7134</eissn><abstract>Extracted nucleic acids were also tested using real-time fluorescent resonance energy transfer (FRET) PCR assays for B. miyamotoi (GlpQ: Forward primer 5'-TCCAGAACATACCTTAGAAGC- 3'; reverse primer 5'-ATCAAATCTTTCACTGAGACTTA-3'; fluorescein-labeled probe 5'-GACAATGTTCCTATTATAATGCACGACCC-fl-3'; 5'-LC640-GAAATTGACACAACCACAAATGTTGCAC-3'), and Powassan virus (NS5: forward primer 5'-ACTAGAATGGCCATGACAGAC-3'; reverse primer 5'-TCATCTCTGGTGCACATCC-3'; fluorescein-labeled probe 5'-CACAAAGGCCCAGGAACCACAGC-fl-3'; red labeled probe 5'-LC640-GGCACCAGIGTGATCATGAGAGCIGT-3'); (WSH site only because ticks were stored in ethanol at WAL). [...]a relatively small number of ticks were analyzed and only nymphs were present in large numbers at the southeastern location (WAL). [...]inconsistent tick storage limited our ability to detect the full spectrum of pathogens. [...]this study demonstrates pathogen prevalence and coinfections in I. scapularis ticks among regions of the upper midwestern United States.</abstract><cop>United States</cop><pub>Society for Vector Ecology</pub><pmid>32492273</pmid><doi>10.1111/jvec.12384</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1081-1710 |
| ispartof | Journal of vector ecology, 2020-06, Vol.45 (1), p.142-144 |
| issn | 1081-1710 1948-7134 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2409645849 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adults Arachnids Energy transfer Ethanol Fluorescein Fluorescence Fluorescence resonance energy transfer Infections Nucleic acids Pathogens SCIENTIFIC NOTES Ticks Viruses |
| title | Detection of zoonotic human pathogens from Ixodes scapularis in Wisconsin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-20T16%3A26%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Detection%20of%20zoonotic%20human%20pathogens%20from%20Ixodes%20scapularis%20in%20Wisconsin&rft.jtitle=Journal%20of%20vector%20ecology&rft.au=Stauffer,%20Maxwell%20T&rft.date=2020-06&rft.volume=45&rft.issue=1&rft.spage=142&rft.epage=144&rft.pages=142-144&rft.issn=1081-1710&rft.eissn=1948-7134&rft_id=info:doi/10.1111/jvec.12384&rft_dat=%3Cproquest_cross%3E2424471480%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2424471480&rft_id=info:pmid/32492273&rfr_iscdi=true |