Restoration of β-Adrenergic Signaling and Activity of Akt-Kinase and AMP-Activated Protein Kinase with Metformin in the Myocardium of Diabetic Rats
We studied the effect of metformin (100 and 200 mg/kg/day, 4 weeks) on the adenylyl cyclasestimulating effects of β-agonists and relaxin in the myocardial membranes and on activities of Akt-kinase, an effector component of insulin signaling, and AMP-activated protein kinase (AMPK), a cellular energy...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2020-05, Vol.169 (1), p.24-28 |
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| container_title | Bulletin of experimental biology and medicine |
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| creator | Derkach, K. V. Bondareva, V. M. Kornyushin, O. V. Galagudza, M. M. Shpakov, A. O. |
| description | We studied the effect of metformin (100 and 200 mg/kg/day, 4 weeks) on the adenylyl cyclasestimulating effects of β-agonists and relaxin in the myocardial membranes and on activities of Akt-kinase, an effector component of insulin signaling, and AMP-activated protein kinase (AMPK), a cellular energy sensor, in the myocardium of rats with type 2 diabetes mellitus induced by high-fat diet and streptozotocin. Metformin normalized the ratio of adenylyl cyclase effects of β
1/2
- and β
3
-agonists in the myocardial membranes, that is reduced in DM2, and restored phosphorylation of Akt-kinase by Ser473 and AMPK by Thr172 in the myocardium of diabetic rats. The effect of metformin in a dose of 200 mg/kg/day was more pronounced. Thus, the cardioprotective effect of metformin is due to its ability to restore the adrenergic and insulin regulation in cardiomyocytes and their energy status. |
| doi_str_mv | 10.1007/s10517-020-04816-7 |
| format | Article |
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1/2
- and β
3
-agonists in the myocardial membranes, that is reduced in DM2, and restored phosphorylation of Akt-kinase by Ser473 and AMPK by Thr172 in the myocardium of diabetic rats. The effect of metformin in a dose of 200 mg/kg/day was more pronounced. Thus, the cardioprotective effect of metformin is due to its ability to restore the adrenergic and insulin regulation in cardiomyocytes and their energy status.</description><identifier>ISSN: 0007-4888</identifier><identifier>EISSN: 1573-8221</identifier><identifier>DOI: 10.1007/s10517-020-04816-7</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adenylate cyclase ; Agonists ; AKT protein ; AMP ; AMP-activated protein kinase ; Antidiabetics ; Biomedical and Life Sciences ; Biomedicine ; Cardiomyocytes ; Cell Biology ; Diabetes ; Diabetes mellitus (non-insulin dependent) ; Energy balance ; High fat diet ; Insulin ; Internal Medicine ; Kinases ; Laboratory Medicine ; Metformin ; Myocardium ; Pathology ; Phosphates ; Phosphorylation ; Relaxin ; Streptozocin</subject><ispartof>Bulletin of experimental biology and medicine, 2020-05, Vol.169 (1), p.24-28</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-9f7d2a183f777b687fefb705f794723cfde3ef95c07a3ced456991ba191110033</citedby><cites>FETCH-LOGICAL-c352t-9f7d2a183f777b687fefb705f794723cfde3ef95c07a3ced456991ba191110033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10517-020-04816-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10517-020-04816-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Derkach, K. V.</creatorcontrib><creatorcontrib>Bondareva, V. M.</creatorcontrib><creatorcontrib>Kornyushin, O. V.</creatorcontrib><creatorcontrib>Galagudza, M. M.</creatorcontrib><creatorcontrib>Shpakov, A. O.</creatorcontrib><title>Restoration of β-Adrenergic Signaling and Activity of Akt-Kinase and AMP-Activated Protein Kinase with Metformin in the Myocardium of Diabetic Rats</title><title>Bulletin of experimental biology and medicine</title><addtitle>Bull Exp Biol Med</addtitle><description>We studied the effect of metformin (100 and 200 mg/kg/day, 4 weeks) on the adenylyl cyclasestimulating effects of β-agonists and relaxin in the myocardial membranes and on activities of Akt-kinase, an effector component of insulin signaling, and AMP-activated protein kinase (AMPK), a cellular energy sensor, in the myocardium of rats with type 2 diabetes mellitus induced by high-fat diet and streptozotocin. Metformin normalized the ratio of adenylyl cyclase effects of β
1/2
- and β
3
-agonists in the myocardial membranes, that is reduced in DM2, and restored phosphorylation of Akt-kinase by Ser473 and AMPK by Thr172 in the myocardium of diabetic rats. The effect of metformin in a dose of 200 mg/kg/day was more pronounced. Thus, the cardioprotective effect of metformin is due to its ability to restore the adrenergic and insulin regulation in cardiomyocytes and their energy status.</description><subject>Adenylate cyclase</subject><subject>Agonists</subject><subject>AKT protein</subject><subject>AMP</subject><subject>AMP-activated protein kinase</subject><subject>Antidiabetics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cardiomyocytes</subject><subject>Cell Biology</subject><subject>Diabetes</subject><subject>Diabetes mellitus (non-insulin dependent)</subject><subject>Energy balance</subject><subject>High fat diet</subject><subject>Insulin</subject><subject>Internal Medicine</subject><subject>Kinases</subject><subject>Laboratory Medicine</subject><subject>Metformin</subject><subject>Myocardium</subject><subject>Pathology</subject><subject>Phosphates</subject><subject>Phosphorylation</subject><subject>Relaxin</subject><subject>Streptozocin</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kcFOGzEQhi1UJNLQF-jJEpdeDPZ6d717jNIWEESg0J4txzsOhsRObaco78GT8CA8E14WCYkDkjWWZ775Lf0_Qt8ZPWaUipPIaMUEoQUltGxYTcQeGrFKcNIUBfuCRjRTpGya5gB9jfGuf9KajdDjHGLyQSXrHfYGPz-RSRfAQVhajW_s0qmVdUusXIcnOtn_Nu16bnKfyIV1KsIwml2T17FK0OHr4BNYh9-AB5tu8QyS8WGdu_mkW8CzndcqdHa77vV-WrWAlL-cqxQP0b5Rqwjf3u4x-vv715_pGbm8Oj2fTi6J5lWRSGtEVyjWcCOEWNSNMGAWglZGtKUouDYdcDBtpalQXENXVnXbsoViLWPZNc7H6Meguwn-3zYbIdc2alitlAO_jbIoaVuXvCxFRo8-oHd-G7I5PcV693PNVDFQOvgYAxi5CXatwk4yKvug5BCUzEHJ16BkL82HpZhht4TwLv3J1gtFYpbt</recordid><startdate>20200501</startdate><enddate>20200501</enddate><creator>Derkach, K. 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Metformin normalized the ratio of adenylyl cyclase effects of β
1/2
- and β
3
-agonists in the myocardial membranes, that is reduced in DM2, and restored phosphorylation of Akt-kinase by Ser473 and AMPK by Thr172 in the myocardium of diabetic rats. The effect of metformin in a dose of 200 mg/kg/day was more pronounced. Thus, the cardioprotective effect of metformin is due to its ability to restore the adrenergic and insulin regulation in cardiomyocytes and their energy status.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s10517-020-04816-7</doi><tpages>5</tpages></addata></record> |
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| subjects | Adenylate cyclase Agonists AKT protein AMP AMP-activated protein kinase Antidiabetics Biomedical and Life Sciences Biomedicine Cardiomyocytes Cell Biology Diabetes Diabetes mellitus (non-insulin dependent) Energy balance High fat diet Insulin Internal Medicine Kinases Laboratory Medicine Metformin Myocardium Pathology Phosphates Phosphorylation Relaxin Streptozocin |
| title | Restoration of β-Adrenergic Signaling and Activity of Akt-Kinase and AMP-Activated Protein Kinase with Metformin in the Myocardium of Diabetic Rats |
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