A novel method to evaluate prethawing viability of cryopreserved CD34+ hematopoietic stem cells for autologous transplantation
BACKGROUND Cryopreservation of CD34+ hematopoietic stem cells (HSCs) is associated with variable loss of viability. Although postfreezing CD34+ cell viability can be assessed on the sampling tube (bag tail) directly connected to the main bag (mother bag), results often underestimate the actual viabi...
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| Veröffentlicht in: | Transfusion (Philadelphia, Pa.) Pa.), 2020-07, Vol.60 (7), p.1529-1535 |
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| creator | Marinelli Busilacchi, Elena Costantini, Andrea Mancini, Giorgia Bencivenga, Rossella Olivieri, Jacopo Battaglini, Giovanna Velletri, Laura Viola, Nadia Butini, Luca Capelli, Debora Poloni, Antonella Olivieri, Attilio |
| description | BACKGROUND
Cryopreservation of CD34+ hematopoietic stem cells (HSCs) is associated with variable loss of viability. Although postfreezing CD34+ cell viability can be assessed on the sampling tube (bag tail) directly connected to the main bag (mother bag), results often underestimate the actual viability observed when the mother bag is thawed and reinfused. We assessed a novel method to measure postfreezing CD34+ cell viability, based on small bag (minibag) samples; results were compared with those obtained on the corresponding mother bags and bag tails.
STUDY DESIGN AND METHODS
Sixty‐one apheresis procedures of 42 patients undergoing autologous HSC transplant were analyzed. Viable CD34+ cells were quantified with flow cytometry before controlled rate freezing (ICE‐CUBE14M system, SY‐LAB‐ IceCube, SIAD), after 10 days of storage (mini‐bag and bag tail), and before reinfusion (aliquot from a thawed mother bag). Results were compared using Studentʼs t test and Spearmanʼs rho correlation test.
RESULTS
The mean CD34+ cell viability before cryopreservation was 99.3% (confidence interval [CI], 98.94‐99.65%); the mean amount of CD34+ cells, white blood cells and neutrophils in the mother bag was 0.8 ± 1.1 × 109/L, 63.4 ± 23.5 × 109/L, and 25.7 ± 15.5 × 109/L, respectively. Mother bags postthawing CD34+ cell viability was 72.3% (CI, 67.74‐76.85%; p |
| doi_str_mv | 10.1111/trf.15825 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2409193677</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2409193677</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3305-7f0761be80cf55a4c48fcac3ff4359eccd5ff7ede5f7fe4d9383f6d5f665406a3</originalsourceid><addsrcrecordid>eNp10ctKxDAUBuAgCo6jC98g4EaRjklz6XQp4xUEQXRdYnqiGdKmJukMs_HZjY4rwWwC53wJP_wIHVMyo_lcpGBmVMxLsYMmVLCqKOta7KIJIZwWlLJyHx3EuCSElDWhE_R5iXu_Aoc7SO--xcljWCk3qgR4CHmm1rZ_wyurXq2zaYO9wTpsfN5FCCto8eKK8XP8Dp1KfvAWktU4JuiwBuciNj5gNSbv_JsfI05B9XFwqk8qWd8foj2jXISj33uKXm6unxd3xcPj7f3i8qHQjBFRVIZUkr7CnGgjhOKaz41WmhnDmahB61YYU0ELwlQGeFuzOTMyD6UUnEjFpuh0--8Q_McIMTWdjd8BVQ85VlNyUtOayarK9OQPXfox9DldViWXkspSZnW2VTr4GAOYZgi2U2HTUNJ8N9HkJpqfJrK92Nq1dbD5HzbPTzfbF1-B441f</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2424661626</pqid></control><display><type>article</type><title>A novel method to evaluate prethawing viability of cryopreserved CD34+ hematopoietic stem cells for autologous transplantation</title><source>Wiley Journals</source><creator>Marinelli Busilacchi, Elena ; Costantini, Andrea ; Mancini, Giorgia ; Bencivenga, Rossella ; Olivieri, Jacopo ; Battaglini, Giovanna ; Velletri, Laura ; Viola, Nadia ; Butini, Luca ; Capelli, Debora ; Poloni, Antonella ; Olivieri, Attilio</creator><creatorcontrib>Marinelli Busilacchi, Elena ; Costantini, Andrea ; Mancini, Giorgia ; Bencivenga, Rossella ; Olivieri, Jacopo ; Battaglini, Giovanna ; Velletri, Laura ; Viola, Nadia ; Butini, Luca ; Capelli, Debora ; Poloni, Antonella ; Olivieri, Attilio</creatorcontrib><description>BACKGROUND
Cryopreservation of CD34+ hematopoietic stem cells (HSCs) is associated with variable loss of viability. Although postfreezing CD34+ cell viability can be assessed on the sampling tube (bag tail) directly connected to the main bag (mother bag), results often underestimate the actual viability observed when the mother bag is thawed and reinfused. We assessed a novel method to measure postfreezing CD34+ cell viability, based on small bag (minibag) samples; results were compared with those obtained on the corresponding mother bags and bag tails.
STUDY DESIGN AND METHODS
Sixty‐one apheresis procedures of 42 patients undergoing autologous HSC transplant were analyzed. Viable CD34+ cells were quantified with flow cytometry before controlled rate freezing (ICE‐CUBE14M system, SY‐LAB‐ IceCube, SIAD), after 10 days of storage (mini‐bag and bag tail), and before reinfusion (aliquot from a thawed mother bag). Results were compared using Studentʼs t test and Spearmanʼs rho correlation test.
RESULTS
The mean CD34+ cell viability before cryopreservation was 99.3% (confidence interval [CI], 98.94‐99.65%); the mean amount of CD34+ cells, white blood cells and neutrophils in the mother bag was 0.8 ± 1.1 × 109/L, 63.4 ± 23.5 × 109/L, and 25.7 ± 15.5 × 109/L, respectively. Mother bags postthawing CD34+ cell viability was 72.3% (CI, 67.74‐76.85%; p < 0.01 compared to prefreezing); no difference was observed with respect to minibags (73.7%; CI, 69.80‐77.59%; p = NS), whereas significantly lower values were found for bag tails (58.6%; CI, 54.19‐63.00%; p < 0.01 vs. both mini‐ and mother bags).
CONCLUSION
Compared to bag tails, minibags represent a more accurate tool to measure the CD34+ cell viability of the apheresis mother bag prior to reinfusion; in addition, minibags may could be of help for case‐by‐case calculation of the amount of apheresis to be infused to patients undergoing autologous HSC transplant.</description><identifier>ISSN: 0041-1132</identifier><identifier>EISSN: 1537-2995</identifier><identifier>DOI: 10.1111/trf.15825</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Apheresis ; Autografts ; CD34 antigen ; Cell viability ; Confidence intervals ; Cryopreservation ; Flow cytometry ; Freezing ; Hematopoietic stem cells ; Leukocytes ; Leukocytes (neutrophilic) ; Stem cells ; Tails ; Transplantation ; Transplants & implants</subject><ispartof>Transfusion (Philadelphia, Pa.), 2020-07, Vol.60 (7), p.1529-1535</ispartof><rights>2020 AABB</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3305-7f0761be80cf55a4c48fcac3ff4359eccd5ff7ede5f7fe4d9383f6d5f665406a3</citedby><cites>FETCH-LOGICAL-c3305-7f0761be80cf55a4c48fcac3ff4359eccd5ff7ede5f7fe4d9383f6d5f665406a3</cites><orcidid>0000-0002-0693-3376</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Ftrf.15825$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Ftrf.15825$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Marinelli Busilacchi, Elena</creatorcontrib><creatorcontrib>Costantini, Andrea</creatorcontrib><creatorcontrib>Mancini, Giorgia</creatorcontrib><creatorcontrib>Bencivenga, Rossella</creatorcontrib><creatorcontrib>Olivieri, Jacopo</creatorcontrib><creatorcontrib>Battaglini, Giovanna</creatorcontrib><creatorcontrib>Velletri, Laura</creatorcontrib><creatorcontrib>Viola, Nadia</creatorcontrib><creatorcontrib>Butini, Luca</creatorcontrib><creatorcontrib>Capelli, Debora</creatorcontrib><creatorcontrib>Poloni, Antonella</creatorcontrib><creatorcontrib>Olivieri, Attilio</creatorcontrib><title>A novel method to evaluate prethawing viability of cryopreserved CD34+ hematopoietic stem cells for autologous transplantation</title><title>Transfusion (Philadelphia, Pa.)</title><description>BACKGROUND
Cryopreservation of CD34+ hematopoietic stem cells (HSCs) is associated with variable loss of viability. Although postfreezing CD34+ cell viability can be assessed on the sampling tube (bag tail) directly connected to the main bag (mother bag), results often underestimate the actual viability observed when the mother bag is thawed and reinfused. We assessed a novel method to measure postfreezing CD34+ cell viability, based on small bag (minibag) samples; results were compared with those obtained on the corresponding mother bags and bag tails.
STUDY DESIGN AND METHODS
Sixty‐one apheresis procedures of 42 patients undergoing autologous HSC transplant were analyzed. Viable CD34+ cells were quantified with flow cytometry before controlled rate freezing (ICE‐CUBE14M system, SY‐LAB‐ IceCube, SIAD), after 10 days of storage (mini‐bag and bag tail), and before reinfusion (aliquot from a thawed mother bag). Results were compared using Studentʼs t test and Spearmanʼs rho correlation test.
RESULTS
The mean CD34+ cell viability before cryopreservation was 99.3% (confidence interval [CI], 98.94‐99.65%); the mean amount of CD34+ cells, white blood cells and neutrophils in the mother bag was 0.8 ± 1.1 × 109/L, 63.4 ± 23.5 × 109/L, and 25.7 ± 15.5 × 109/L, respectively. Mother bags postthawing CD34+ cell viability was 72.3% (CI, 67.74‐76.85%; p < 0.01 compared to prefreezing); no difference was observed with respect to minibags (73.7%; CI, 69.80‐77.59%; p = NS), whereas significantly lower values were found for bag tails (58.6%; CI, 54.19‐63.00%; p < 0.01 vs. both mini‐ and mother bags).
CONCLUSION
Compared to bag tails, minibags represent a more accurate tool to measure the CD34+ cell viability of the apheresis mother bag prior to reinfusion; in addition, minibags may could be of help for case‐by‐case calculation of the amount of apheresis to be infused to patients undergoing autologous HSC transplant.</description><subject>Apheresis</subject><subject>Autografts</subject><subject>CD34 antigen</subject><subject>Cell viability</subject><subject>Confidence intervals</subject><subject>Cryopreservation</subject><subject>Flow cytometry</subject><subject>Freezing</subject><subject>Hematopoietic stem cells</subject><subject>Leukocytes</subject><subject>Leukocytes (neutrophilic)</subject><subject>Stem cells</subject><subject>Tails</subject><subject>Transplantation</subject><subject>Transplants & implants</subject><issn>0041-1132</issn><issn>1537-2995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp10ctKxDAUBuAgCo6jC98g4EaRjklz6XQp4xUEQXRdYnqiGdKmJukMs_HZjY4rwWwC53wJP_wIHVMyo_lcpGBmVMxLsYMmVLCqKOta7KIJIZwWlLJyHx3EuCSElDWhE_R5iXu_Aoc7SO--xcljWCk3qgR4CHmm1rZ_wyurXq2zaYO9wTpsfN5FCCto8eKK8XP8Dp1KfvAWktU4JuiwBuciNj5gNSbv_JsfI05B9XFwqk8qWd8foj2jXISj33uKXm6unxd3xcPj7f3i8qHQjBFRVIZUkr7CnGgjhOKaz41WmhnDmahB61YYU0ELwlQGeFuzOTMyD6UUnEjFpuh0--8Q_McIMTWdjd8BVQ85VlNyUtOayarK9OQPXfox9DldViWXkspSZnW2VTr4GAOYZgi2U2HTUNJ8N9HkJpqfJrK92Nq1dbD5HzbPTzfbF1-B441f</recordid><startdate>202007</startdate><enddate>202007</enddate><creator>Marinelli Busilacchi, Elena</creator><creator>Costantini, Andrea</creator><creator>Mancini, Giorgia</creator><creator>Bencivenga, Rossella</creator><creator>Olivieri, Jacopo</creator><creator>Battaglini, Giovanna</creator><creator>Velletri, Laura</creator><creator>Viola, Nadia</creator><creator>Butini, Luca</creator><creator>Capelli, Debora</creator><creator>Poloni, Antonella</creator><creator>Olivieri, Attilio</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0693-3376</orcidid></search><sort><creationdate>202007</creationdate><title>A novel method to evaluate prethawing viability of cryopreserved CD34+ hematopoietic stem cells for autologous transplantation</title><author>Marinelli Busilacchi, Elena ; Costantini, Andrea ; Mancini, Giorgia ; Bencivenga, Rossella ; Olivieri, Jacopo ; Battaglini, Giovanna ; Velletri, Laura ; Viola, Nadia ; Butini, Luca ; Capelli, Debora ; Poloni, Antonella ; Olivieri, Attilio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3305-7f0761be80cf55a4c48fcac3ff4359eccd5ff7ede5f7fe4d9383f6d5f665406a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Apheresis</topic><topic>Autografts</topic><topic>CD34 antigen</topic><topic>Cell viability</topic><topic>Confidence intervals</topic><topic>Cryopreservation</topic><topic>Flow cytometry</topic><topic>Freezing</topic><topic>Hematopoietic stem cells</topic><topic>Leukocytes</topic><topic>Leukocytes (neutrophilic)</topic><topic>Stem cells</topic><topic>Tails</topic><topic>Transplantation</topic><topic>Transplants & implants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marinelli Busilacchi, Elena</creatorcontrib><creatorcontrib>Costantini, Andrea</creatorcontrib><creatorcontrib>Mancini, Giorgia</creatorcontrib><creatorcontrib>Bencivenga, Rossella</creatorcontrib><creatorcontrib>Olivieri, Jacopo</creatorcontrib><creatorcontrib>Battaglini, Giovanna</creatorcontrib><creatorcontrib>Velletri, Laura</creatorcontrib><creatorcontrib>Viola, Nadia</creatorcontrib><creatorcontrib>Butini, Luca</creatorcontrib><creatorcontrib>Capelli, Debora</creatorcontrib><creatorcontrib>Poloni, Antonella</creatorcontrib><creatorcontrib>Olivieri, Attilio</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transfusion (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marinelli Busilacchi, Elena</au><au>Costantini, Andrea</au><au>Mancini, Giorgia</au><au>Bencivenga, Rossella</au><au>Olivieri, Jacopo</au><au>Battaglini, Giovanna</au><au>Velletri, Laura</au><au>Viola, Nadia</au><au>Butini, Luca</au><au>Capelli, Debora</au><au>Poloni, Antonella</au><au>Olivieri, Attilio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel method to evaluate prethawing viability of cryopreserved CD34+ hematopoietic stem cells for autologous transplantation</atitle><jtitle>Transfusion (Philadelphia, Pa.)</jtitle><date>2020-07</date><risdate>2020</risdate><volume>60</volume><issue>7</issue><spage>1529</spage><epage>1535</epage><pages>1529-1535</pages><issn>0041-1132</issn><eissn>1537-2995</eissn><abstract>BACKGROUND
Cryopreservation of CD34+ hematopoietic stem cells (HSCs) is associated with variable loss of viability. Although postfreezing CD34+ cell viability can be assessed on the sampling tube (bag tail) directly connected to the main bag (mother bag), results often underestimate the actual viability observed when the mother bag is thawed and reinfused. We assessed a novel method to measure postfreezing CD34+ cell viability, based on small bag (minibag) samples; results were compared with those obtained on the corresponding mother bags and bag tails.
STUDY DESIGN AND METHODS
Sixty‐one apheresis procedures of 42 patients undergoing autologous HSC transplant were analyzed. Viable CD34+ cells were quantified with flow cytometry before controlled rate freezing (ICE‐CUBE14M system, SY‐LAB‐ IceCube, SIAD), after 10 days of storage (mini‐bag and bag tail), and before reinfusion (aliquot from a thawed mother bag). Results were compared using Studentʼs t test and Spearmanʼs rho correlation test.
RESULTS
The mean CD34+ cell viability before cryopreservation was 99.3% (confidence interval [CI], 98.94‐99.65%); the mean amount of CD34+ cells, white blood cells and neutrophils in the mother bag was 0.8 ± 1.1 × 109/L, 63.4 ± 23.5 × 109/L, and 25.7 ± 15.5 × 109/L, respectively. Mother bags postthawing CD34+ cell viability was 72.3% (CI, 67.74‐76.85%; p < 0.01 compared to prefreezing); no difference was observed with respect to minibags (73.7%; CI, 69.80‐77.59%; p = NS), whereas significantly lower values were found for bag tails (58.6%; CI, 54.19‐63.00%; p < 0.01 vs. both mini‐ and mother bags).
CONCLUSION
Compared to bag tails, minibags represent a more accurate tool to measure the CD34+ cell viability of the apheresis mother bag prior to reinfusion; in addition, minibags may could be of help for case‐by‐case calculation of the amount of apheresis to be infused to patients undergoing autologous HSC transplant.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><doi>10.1111/trf.15825</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-0693-3376</orcidid></addata></record> |
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| ispartof | Transfusion (Philadelphia, Pa.), 2020-07, Vol.60 (7), p.1529-1535 |
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| language | eng |
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| source | Wiley Journals |
| subjects | Apheresis Autografts CD34 antigen Cell viability Confidence intervals Cryopreservation Flow cytometry Freezing Hematopoietic stem cells Leukocytes Leukocytes (neutrophilic) Stem cells Tails Transplantation Transplants & implants |
| title | A novel method to evaluate prethawing viability of cryopreserved CD34+ hematopoietic stem cells for autologous transplantation |
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