Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum
The purpose of this study was to study polysaccharides isolated from Polygonatum sibiricum to establish the structure-activity relationships of the active substances and to discover the optimal fraction for further development and application. Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP...
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Veröffentlicht in: | International journal of biological macromolecules 2020-10, Vol.160, p.688-694 |
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creator | Wang, Yongjie Liu, Na Xue, Xia Li, Qiang Sun, Deqing Zhao, Zhongxi |
description | The purpose of this study was to study polysaccharides isolated from Polygonatum sibiricum to establish the structure-activity relationships of the active substances and to discover the optimal fraction for further development and application. Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography. Acid hydrolysis and FT-IR spectral and NMR spectral analyses were employed for structural analysis. Our results illustrated that PSP with different chemical structure and monosaccharide composition showed different abilities to activate phagocytic activity in vitro. According to the preliminary screening results in vitro, the newly identified water-soluble polysaccharides of PSP3 were selected for further evaluation in vivo. The results demonstrated that PSP3 possessed an immunomodulatory function and could be regarded as a promising candidate as an immunomodulator. |
doi_str_mv | 10.1016/j.ijbiomac.2020.05.245 |
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Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography. Acid hydrolysis and FT-IR spectral and NMR spectral analyses were employed for structural analysis. Our results illustrated that PSP with different chemical structure and monosaccharide composition showed different abilities to activate phagocytic activity in vitro. According to the preliminary screening results in vitro, the newly identified water-soluble polysaccharides of PSP3 were selected for further evaluation in vivo. The results demonstrated that PSP3 possessed an immunomodulatory function and could be regarded as a promising candidate as an immunomodulator.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2020.05.245</identifier><identifier>PMID: 32479947</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Active substances ; Ethanolamines - chemistry ; Immunologic Factors - chemistry ; Immunostimulatory activity ; Magnetic Resonance Spectroscopy ; Polygonatum - chemistry ; Polygonatum sibiricum polysaccharides ; Polysaccharides - chemistry ; Sepharose - chemistry ; Spectroscopy, Fourier Transform Infrared</subject><ispartof>International journal of biological macromolecules, 2020-10, Vol.160, p.688-694</ispartof><rights>2020</rights><rights>Copyright © 2020. 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The results demonstrated that PSP3 possessed an immunomodulatory function and could be regarded as a promising candidate as an immunomodulator.</description><subject>Active substances</subject><subject>Ethanolamines - chemistry</subject><subject>Immunologic Factors - chemistry</subject><subject>Immunostimulatory activity</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Polygonatum - chemistry</subject><subject>Polygonatum sibiricum polysaccharides</subject><subject>Polysaccharides - chemistry</subject><subject>Sepharose - chemistry</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1TAQhS0EoreFv1B5yYIEv-I4O1DFS6pEF7C2HNspcxXHFz8i3a754eRyW7asRjrznRnNHISuKWkpofLdvoX9CDEY2zLCSEu6lonuGdpR1Q8NIYQ_RztCBW0U5eQCXea831TZUfUSXXAm-mEQ_Q79vqsJJrCmQFze4lxStaUmM2P70yRji0_w8LeJzeIwLHiFNWIIoS4x-fs6mxLTEW8krFCOOE7Y4CWufsaHOB-zsadB4DyeUgz4btPu42JKDTjDCAlsDa_Qi8nM2b9-rFfox6eP32--NLffPn-9-XDbWC5VaVQnGWfOjpyMopfMOMUpF_0oFB24YL2ljPSEeTMNlpPJSdqP3AlJRy8FV_wKvTnPPaT4q_pcdIBs_TybxceaNRNEKSYEO6HyjNoUc05-0ocEwaSjpkSfEtB7_ZSAPiWgSbf5u814_bijjsG7f7anl2_A-zPgt0tX8ElnC36x3kHytmgX4X87_gAhep3F</recordid><startdate>20201001</startdate><enddate>20201001</enddate><creator>Wang, Yongjie</creator><creator>Liu, Na</creator><creator>Xue, Xia</creator><creator>Li, Qiang</creator><creator>Sun, Deqing</creator><creator>Zhao, Zhongxi</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20201001</creationdate><title>Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum</title><author>Wang, Yongjie ; Liu, Na ; Xue, Xia ; Li, Qiang ; Sun, Deqing ; Zhao, Zhongxi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-856232dcb30b4762ad831347b48193427c120702eaf9c30fd617b3d461be64383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Active substances</topic><topic>Ethanolamines - chemistry</topic><topic>Immunologic Factors - chemistry</topic><topic>Immunostimulatory activity</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Polygonatum - chemistry</topic><topic>Polygonatum sibiricum polysaccharides</topic><topic>Polysaccharides - chemistry</topic><topic>Sepharose - chemistry</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yongjie</creatorcontrib><creatorcontrib>Liu, Na</creatorcontrib><creatorcontrib>Xue, Xia</creatorcontrib><creatorcontrib>Li, Qiang</creatorcontrib><creatorcontrib>Sun, Deqing</creatorcontrib><creatorcontrib>Zhao, Zhongxi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yongjie</au><au>Liu, Na</au><au>Xue, Xia</au><au>Li, Qiang</au><au>Sun, Deqing</au><au>Zhao, Zhongxi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2020-10-01</date><risdate>2020</risdate><volume>160</volume><spage>688</spage><epage>694</epage><pages>688-694</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>The purpose of this study was to study polysaccharides isolated from Polygonatum sibiricum to establish the structure-activity relationships of the active substances and to discover the optimal fraction for further development and application. Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography. Acid hydrolysis and FT-IR spectral and NMR spectral analyses were employed for structural analysis. Our results illustrated that PSP with different chemical structure and monosaccharide composition showed different abilities to activate phagocytic activity in vitro. According to the preliminary screening results in vitro, the newly identified water-soluble polysaccharides of PSP3 were selected for further evaluation in vivo. The results demonstrated that PSP3 possessed an immunomodulatory function and could be regarded as a promising candidate as an immunomodulator.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>32479947</pmid><doi>10.1016/j.ijbiomac.2020.05.245</doi><tpages>7</tpages></addata></record> |
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subjects | Active substances Ethanolamines - chemistry Immunologic Factors - chemistry Immunostimulatory activity Magnetic Resonance Spectroscopy Polygonatum - chemistry Polygonatum sibiricum polysaccharides Polysaccharides - chemistry Sepharose - chemistry Spectroscopy, Fourier Transform Infrared |
title | Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum |
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