Production of polyhydroxyalkanoate from acetate by metabolically engineered Aeromonas hydrophilia

Aeromonas hydrophila 4AK4 normally produces the copolymer of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx) using lauric acid as the carbon source. In this study we reported the metabolic engineering of A. hydrophila 4AK4 for the production of polyhydroxyalkanoate (PHA) using acetate as a main ca...

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Veröffentlicht in:Journal of bioscience and bioengineering 2020-09, Vol.130 (3), p.290-294
Hauptverfasser: Shi, Li-Long, Da, Yang-Yang, Zheng, Wei-Tao, Chen, Guo-Qiang, Li, Zheng-Jun
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container_end_page 294
container_issue 3
container_start_page 290
container_title Journal of bioscience and bioengineering
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creator Shi, Li-Long
Da, Yang-Yang
Zheng, Wei-Tao
Chen, Guo-Qiang
Li, Zheng-Jun
description Aeromonas hydrophila 4AK4 normally produces the copolymer of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx) using lauric acid as the carbon source. In this study we reported the metabolic engineering of A. hydrophila 4AK4 for the production of polyhydroxyalkanoate (PHA) using acetate as a main carbon source. Recombinant A. hydrophila overexpressing β-ketothiolase and acetoacetyl-CoA reductase could accumulate poly-3-hydroxybutyrate (PHB) from acetate with a polymer content of 1.39 wt%. Further overexpression of acetate kinase/phosphotransacetylase and acetyl-CoA synthetase improved PHB content to 8.75 wt% and 19.82 wt%, respectively. When acetate and propionate were simultaneously supplied as carbon sources, the engineered A. hydrophila overexpressing β-ketothiolase, acetoacetyl-CoA reductase, and acetyl-CoA synthetase was found able to produce the copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate (PHBV). The recombinant grew to 3.79 g/L cell dry weight (CDW) containing 15.02 wt% PHBV. Our proposed metabolic engineering strategies illustrate the feasibility for producing PHA from acetate by A. hydrophila.
doi_str_mv 10.1016/j.jbiosc.2020.05.003
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subjects Acetate
Acetyl-CoA synthetase
Aeromonas hydrophila
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
Poly-3-hydroxybutyrate
title Production of polyhydroxyalkanoate from acetate by metabolically engineered Aeromonas hydrophilia
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