MiR-204-5p/TFAP2A feedback loop positively regulates the proliferation, migration, invasion and EMT process in cervical cancer
MicroRNAs (MiRNAs) have been clarified as crucial regulators of the pathological processes in various carcinomas in the past years. Interestingly, existing evidence has manifested that microRNA-204-5p (miR-204-5p) is engaged in the initiation and progression of multiple carcinomas. However, the pote...
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| Veröffentlicht in: | Cancer biomarkers : section A of Disease markers 2020-01, Vol.28 (3), p.381-390 |
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| description | MicroRNAs (MiRNAs) have been clarified as crucial regulators of the pathological processes in various carcinomas in the past years. Interestingly, existing evidence has manifested that microRNA-204-5p (miR-204-5p) is engaged in the initiation and progression of multiple carcinomas. However, the potential of miR-204-5p in cervical cancer remains to be disentombed. This study focused on unraveling the detailed role of miR-204-5p in cervical cancer. MiR-204-5p exhibited a low level in cervical cancer cells. The functional assays demonstrated that miR-204-5p upregulation exerted suppressive impact on the functions of cervical cancer cells, including proliferation, invasion, migration and epithelial-mesenchymal transition (EMT) process. Moreover, transcription factor AP-2 alpha (TFAP2A) was screened to be the most affected target gene by miR-204-5p, and TFAP2A was discovered to transcriptionally repress miR-204-5p in cervical cancer. The mutual regulation between TFAP2A and miR-204-5p was testified through molecular mechanism assays. Final rescued-function assays demonstrated that overexpression of TFAP2A could recover the suppressed cellular process caused by miR-204-5p upregulation. In conclusion, miR-204-5p/TFAP2A feedback loop promoted the proliferative and motorial capacities of cervical cancer cells. This finding suggested a novel modulatory loop of miR-204-5p/TFAP2A in cervical cancer, offering promising biomarkers for cervical cancer therapy. |
| doi_str_mv | 10.3233/CBM-191064 |
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Interestingly, existing evidence has manifested that microRNA-204-5p (miR-204-5p) is engaged in the initiation and progression of multiple carcinomas. However, the potential of miR-204-5p in cervical cancer remains to be disentombed. This study focused on unraveling the detailed role of miR-204-5p in cervical cancer. MiR-204-5p exhibited a low level in cervical cancer cells. The functional assays demonstrated that miR-204-5p upregulation exerted suppressive impact on the functions of cervical cancer cells, including proliferation, invasion, migration and epithelial-mesenchymal transition (EMT) process. Moreover, transcription factor AP-2 alpha (TFAP2A) was screened to be the most affected target gene by miR-204-5p, and TFAP2A was discovered to transcriptionally repress miR-204-5p in cervical cancer. The mutual regulation between TFAP2A and miR-204-5p was testified through molecular mechanism assays. Final rescued-function assays demonstrated that overexpression of TFAP2A could recover the suppressed cellular process caused by miR-204-5p upregulation. In conclusion, miR-204-5p/TFAP2A feedback loop promoted the proliferative and motorial capacities of cervical cancer cells. This finding suggested a novel modulatory loop of miR-204-5p/TFAP2A in cervical cancer, offering promising biomarkers for cervical cancer therapy.</description><identifier>ISSN: 1574-0153</identifier><identifier>EISSN: 1875-8592</identifier><identifier>DOI: 10.3233/CBM-191064</identifier><identifier>PMID: 32474464</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>AP-2 protein ; Assaying ; Biomarkers ; Cancer ; Carcinoma ; Cell Line, Tumor ; Cell migration ; Cell Movement - genetics ; Cell proliferation ; Cell Proliferation - genetics ; Cervical cancer ; Cervix ; Chromosome 5 ; Epithelial-Mesenchymal Transition - genetics ; Feedback ; Feedback loops ; Feedback, Physiological ; Female ; Functionals ; Gain of Function Mutation ; Gene Expression Regulation, Neoplastic ; Humans ; Low level ; Mesenchyme ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miRNA ; Neoplasm Invasiveness - genetics ; Regulators ; Ribonucleic acid ; RNA ; Tetrahydroisoquinolines ; Transcription Factor AP-2 - genetics ; Transcription Factor AP-2 - metabolism ; Uterine Cervical Neoplasms - genetics ; Uterine Cervical Neoplasms - pathology</subject><ispartof>Cancer biomarkers : section A of Disease markers, 2020-01, Vol.28 (3), p.381-390</ispartof><rights>2020 – IOS Press and the authors. All rights reserved</rights><rights>Copyright IOS Press BV 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c347t-bb26960423a73b1278b14c984cb856774d4c0a33aaa69c8c1e5f3778c2c86e383</citedby><cites>FETCH-LOGICAL-c347t-bb26960423a73b1278b14c984cb856774d4c0a33aaa69c8c1e5f3778c2c86e383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.3233/CBM-191064$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.3233/CBM-191064$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21945,27830,27901,27902,44921,45309</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.3233/CBM-191064?utm_source=summon&utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32474464$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Pei</creatorcontrib><creatorcontrib>Hou, Qingxia</creatorcontrib><creatorcontrib>Yue, Qingfen</creatorcontrib><title>MiR-204-5p/TFAP2A feedback loop positively regulates the proliferation, migration, invasion and EMT process in cervical cancer</title><title>Cancer biomarkers : section A of Disease markers</title><addtitle>Cancer Biomark</addtitle><description>MicroRNAs (MiRNAs) have been clarified as crucial regulators of the pathological processes in various carcinomas in the past years. Interestingly, existing evidence has manifested that microRNA-204-5p (miR-204-5p) is engaged in the initiation and progression of multiple carcinomas. However, the potential of miR-204-5p in cervical cancer remains to be disentombed. This study focused on unraveling the detailed role of miR-204-5p in cervical cancer. MiR-204-5p exhibited a low level in cervical cancer cells. The functional assays demonstrated that miR-204-5p upregulation exerted suppressive impact on the functions of cervical cancer cells, including proliferation, invasion, migration and epithelial-mesenchymal transition (EMT) process. Moreover, transcription factor AP-2 alpha (TFAP2A) was screened to be the most affected target gene by miR-204-5p, and TFAP2A was discovered to transcriptionally repress miR-204-5p in cervical cancer. The mutual regulation between TFAP2A and miR-204-5p was testified through molecular mechanism assays. Final rescued-function assays demonstrated that overexpression of TFAP2A could recover the suppressed cellular process caused by miR-204-5p upregulation. In conclusion, miR-204-5p/TFAP2A feedback loop promoted the proliferative and motorial capacities of cervical cancer cells. This finding suggested a novel modulatory loop of miR-204-5p/TFAP2A in cervical cancer, offering promising biomarkers for cervical cancer therapy.</description><subject>AP-2 protein</subject><subject>Assaying</subject><subject>Biomarkers</subject><subject>Cancer</subject><subject>Carcinoma</subject><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Cell Movement - genetics</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - genetics</subject><subject>Cervical cancer</subject><subject>Cervix</subject><subject>Chromosome 5</subject><subject>Epithelial-Mesenchymal Transition - genetics</subject><subject>Feedback</subject><subject>Feedback loops</subject><subject>Feedback, Physiological</subject><subject>Female</subject><subject>Functionals</subject><subject>Gain of Function Mutation</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Low level</subject><subject>Mesenchyme</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>Neoplasm Invasiveness - genetics</subject><subject>Regulators</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Tetrahydroisoquinolines</subject><subject>Transcription Factor AP-2 - genetics</subject><subject>Transcription Factor AP-2 - metabolism</subject><subject>Uterine Cervical Neoplasms - genetics</subject><subject>Uterine Cervical Neoplasms - pathology</subject><issn>1574-0153</issn><issn>1875-8592</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0ctq3DAUBmBRGppLu-kDFEEXDSFudDmW5OVkmFxghoQwXRtZczxV6rFdyR7IJs8ehUlSKFnpB32cc-An5CtnP6WQ8mx6vsh4wZmCD-SAG51nJi_Ex5RzDRnjudwnhzHeMwaSi-IT2ZcCNICCA_K48HeZYJDl_dnyYnIrJrRGXFXW_aFN1_W076If_BabBxpwPTZ2wEiH30j70DW-xmAH37WndOPXr9G3WxtTorZd0dli-Uwdxpg-qMOw9c421Nk25c9kr7ZNxC8v7xH5dTFbTq-y-c3l9XQyz5wEPWRVJVShGAhptay40Kbi4AoDrjK50hpW4JiV0lqrCmccx7yWWhsnnFEojTwix7u56ZS_I8ah3PjosGlsi90YSwHM5MBNAYl-_4_ed2No03VJCaaF0lwldbJTLnQxBqzLPviNDQ8lZ-VzK2Vqpdy1kvC3l5FjtcHVG32tIYEfOxDtGv_te2fUE8AUkZk</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>Zhang, Pei</creator><creator>Hou, Qingxia</creator><creator>Yue, Qingfen</creator><general>SAGE Publications</general><general>IOS Press BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20200101</creationdate><title>MiR-204-5p/TFAP2A feedback loop positively regulates the proliferation, migration, invasion and EMT process in cervical cancer</title><author>Zhang, Pei ; Hou, Qingxia ; Yue, Qingfen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-bb26960423a73b1278b14c984cb856774d4c0a33aaa69c8c1e5f3778c2c86e383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>AP-2 protein</topic><topic>Assaying</topic><topic>Biomarkers</topic><topic>Cancer</topic><topic>Carcinoma</topic><topic>Cell Line, Tumor</topic><topic>Cell migration</topic><topic>Cell Movement - genetics</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - genetics</topic><topic>Cervical cancer</topic><topic>Cervix</topic><topic>Chromosome 5</topic><topic>Epithelial-Mesenchymal Transition - genetics</topic><topic>Feedback</topic><topic>Feedback loops</topic><topic>Feedback, Physiological</topic><topic>Female</topic><topic>Functionals</topic><topic>Gain of Function Mutation</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Low level</topic><topic>Mesenchyme</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miRNA</topic><topic>Neoplasm Invasiveness - genetics</topic><topic>Regulators</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Tetrahydroisoquinolines</topic><topic>Transcription Factor AP-2 - genetics</topic><topic>Transcription Factor AP-2 - metabolism</topic><topic>Uterine Cervical Neoplasms - genetics</topic><topic>Uterine Cervical Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Pei</creatorcontrib><creatorcontrib>Hou, Qingxia</creatorcontrib><creatorcontrib>Yue, Qingfen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer biomarkers : section A of Disease markers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Zhang, Pei</au><au>Hou, Qingxia</au><au>Yue, Qingfen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MiR-204-5p/TFAP2A feedback loop positively regulates the proliferation, migration, invasion and EMT process in cervical cancer</atitle><jtitle>Cancer biomarkers : section A of Disease markers</jtitle><addtitle>Cancer Biomark</addtitle><date>2020-01-01</date><risdate>2020</risdate><volume>28</volume><issue>3</issue><spage>381</spage><epage>390</epage><pages>381-390</pages><issn>1574-0153</issn><eissn>1875-8592</eissn><abstract>MicroRNAs (MiRNAs) have been clarified as crucial regulators of the pathological processes in various carcinomas in the past years. Interestingly, existing evidence has manifested that microRNA-204-5p (miR-204-5p) is engaged in the initiation and progression of multiple carcinomas. However, the potential of miR-204-5p in cervical cancer remains to be disentombed. This study focused on unraveling the detailed role of miR-204-5p in cervical cancer. MiR-204-5p exhibited a low level in cervical cancer cells. The functional assays demonstrated that miR-204-5p upregulation exerted suppressive impact on the functions of cervical cancer cells, including proliferation, invasion, migration and epithelial-mesenchymal transition (EMT) process. Moreover, transcription factor AP-2 alpha (TFAP2A) was screened to be the most affected target gene by miR-204-5p, and TFAP2A was discovered to transcriptionally repress miR-204-5p in cervical cancer. The mutual regulation between TFAP2A and miR-204-5p was testified through molecular mechanism assays. Final rescued-function assays demonstrated that overexpression of TFAP2A could recover the suppressed cellular process caused by miR-204-5p upregulation. In conclusion, miR-204-5p/TFAP2A feedback loop promoted the proliferative and motorial capacities of cervical cancer cells. This finding suggested a novel modulatory loop of miR-204-5p/TFAP2A in cervical cancer, offering promising biomarkers for cervical cancer therapy.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>32474464</pmid><doi>10.3233/CBM-191064</doi><tpages>10</tpages></addata></record> |
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| ispartof | Cancer biomarkers : section A of Disease markers, 2020-01, Vol.28 (3), p.381-390 |
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| source | Sage Journals GOLD Open Access 2024 |
| subjects | AP-2 protein Assaying Biomarkers Cancer Carcinoma Cell Line, Tumor Cell migration Cell Movement - genetics Cell proliferation Cell Proliferation - genetics Cervical cancer Cervix Chromosome 5 Epithelial-Mesenchymal Transition - genetics Feedback Feedback loops Feedback, Physiological Female Functionals Gain of Function Mutation Gene Expression Regulation, Neoplastic Humans Low level Mesenchyme MicroRNAs MicroRNAs - genetics MicroRNAs - metabolism miRNA Neoplasm Invasiveness - genetics Regulators Ribonucleic acid RNA Tetrahydroisoquinolines Transcription Factor AP-2 - genetics Transcription Factor AP-2 - metabolism Uterine Cervical Neoplasms - genetics Uterine Cervical Neoplasms - pathology |
| title | MiR-204-5p/TFAP2A feedback loop positively regulates the proliferation, migration, invasion and EMT process in cervical cancer |
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