Unambiguous determination of farnesol and tyrosol in vaginal fluid using fast and sensitive UHPLC-MS/MS method
The new ultra-high performance liquid chromatography method with tandem mass spectrometry detection (UHPLC-MS/MS) has been optimized to allow fast, selective, and high-throughput analysis of two Candida albicans quorum sensing molecules (QSM), farnesol and tyrosol. The problem of the presence of the...
Gespeichert in:
| Veröffentlicht in: | Analytical and bioanalytical chemistry 2020-09, Vol.412 (24), p.6529-6541 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 6541 |
|---|---|
| container_issue | 24 |
| container_start_page | 6529 |
| container_title | Analytical and bioanalytical chemistry |
| container_volume | 412 |
| creator | Pilařová, Veronika Kočová Vlčková, Hana Jung, Ondřej Protti, Michele Buchta, Vladimír Mercolini, Laura Svec, Frantisek Nováková, Lucie |
| description | The new ultra-high performance liquid chromatography method with tandem mass spectrometry detection (UHPLC-MS/MS) has been optimized to allow fast, selective, and high-throughput analysis of two
Candida albicans
quorum sensing molecules (QSM), farnesol and tyrosol. The problem of the presence of the interference in the samples and system was successfully solved by careful optimization of chromatographic conditions. Charged hybrid stationary phase modified with pentafluorophenyl group and optimized gradient elution provided adequate separation selectivity and peak shapes. The impurity was identified as dibutyl phthalate and had the same
m/z
ions as farnesol leading to an important interference on selected reaction monitoring channel. Two different types of biological matrices originating from vaginal fluid, supernatant and sediment, were analysed. Micro-solid phase extraction in pipette tips was optimized for the selective isolation of QSM from the supernatant. The insufficient retention of farnesol on the extraction sorbent was improved when 1% of organic solvent was added prior to extraction, while the retention of tyrosol was only possible when using combined C8 and polymer sorbent type. Strong retention of farnesol had to be solved by increasing elution solvent strength and volume up to 600 μL. However, this approach did not allow the pretreatment of sediment samples due to the sorbent clogging. Therefore, our previously developed protein precipitation method was modified and validated to analyse the sediments. New developed UHPLC-MS/MS method provided suitable accuracy and precision for the determination of QSM in vaginal fluid while using only 50 μL sample volume and two different sample preparation methods. |
| doi_str_mv | 10.1007/s00216-020-02699-1 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2408207586</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A633245183</galeid><sourcerecordid>A633245183</sourcerecordid><originalsourceid>FETCH-LOGICAL-c545t-67e0caa00e8ae39325f9f905dd66f817135c6f524949083fc366d1df5afe05613</originalsourceid><addsrcrecordid>eNp9kV1rFTEQhoMo9kP_gBcS8MabbSfJJrt7WQ7aCqco1HMd0s1kTdlNarJb6L8321NbFJEQMkyed5iZl5B3DE4YQHOaAThTFXAoV3VdxV6QQ6ZYW3El4eVTXPMDcpTzDQCTLVOvyYHgtWp50x2SsAtmuvbDEpdMLc6YJh_M7GOg0VFnUsAcR2qCpfN9imvsA70zQ6FG6sbFW7pkH4bC5vmByxiyn_0d0t3Ft-2murw6vbyiE84_on1DXjkzZnz7-B6T3edP3zcX1fbr-ZfN2bbqZS3nSjUIvTEA2BoUneDSda4Daa1SrmUNE7JXTvK6qztoheuFUpZZJ41DkIqJY_JxX_c2xZ8L5llPPvc4jiZgmVTzGloOjWxVQT_8hd7EJZXpVkrITjSyZs_UYEbUPrg4J9OvRfWZEmWfkrWiUCf_oMqxOPk-BnS-5P8Q8L2gL7vNCZ2-TX4y6V4z0KvJem-yLibrB5P12sv7x46X6wntk-S3qwUQeyCXrzBgeh7pP2V_ATawr7A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2435937541</pqid></control><display><type>article</type><title>Unambiguous determination of farnesol and tyrosol in vaginal fluid using fast and sensitive UHPLC-MS/MS method</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Pilařová, Veronika ; Kočová Vlčková, Hana ; Jung, Ondřej ; Protti, Michele ; Buchta, Vladimír ; Mercolini, Laura ; Svec, Frantisek ; Nováková, Lucie</creator><creatorcontrib>Pilařová, Veronika ; Kočová Vlčková, Hana ; Jung, Ondřej ; Protti, Michele ; Buchta, Vladimír ; Mercolini, Laura ; Svec, Frantisek ; Nováková, Lucie</creatorcontrib><description>The new ultra-high performance liquid chromatography method with tandem mass spectrometry detection (UHPLC-MS/MS) has been optimized to allow fast, selective, and high-throughput analysis of two
Candida albicans
quorum sensing molecules (QSM), farnesol and tyrosol. The problem of the presence of the interference in the samples and system was successfully solved by careful optimization of chromatographic conditions. Charged hybrid stationary phase modified with pentafluorophenyl group and optimized gradient elution provided adequate separation selectivity and peak shapes. The impurity was identified as dibutyl phthalate and had the same
m/z
ions as farnesol leading to an important interference on selected reaction monitoring channel. Two different types of biological matrices originating from vaginal fluid, supernatant and sediment, were analysed. Micro-solid phase extraction in pipette tips was optimized for the selective isolation of QSM from the supernatant. The insufficient retention of farnesol on the extraction sorbent was improved when 1% of organic solvent was added prior to extraction, while the retention of tyrosol was only possible when using combined C8 and polymer sorbent type. Strong retention of farnesol had to be solved by increasing elution solvent strength and volume up to 600 μL. However, this approach did not allow the pretreatment of sediment samples due to the sorbent clogging. Therefore, our previously developed protein precipitation method was modified and validated to analyse the sediments. New developed UHPLC-MS/MS method provided suitable accuracy and precision for the determination of QSM in vaginal fluid while using only 50 μL sample volume and two different sample preparation methods.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-020-02699-1</identifier><identifier>PMID: 32468279</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adult ; Alcohols ; Analysis ; Analytical Chemistry ; Biochemistry ; Body fluids ; Candida albicans - isolation & purification ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chromatography, High Pressure Liquid - methods ; Composition ; Dibutyl phthalate ; Elution ; Farnesol ; Farnesol - analysis ; Female ; Female Role Models in Analytical Chemistry ; Food Science ; High performance liquid chromatography ; Humans ; Identification and classification ; Interference ; Ions ; Laboratory Medicine ; Limit of Detection ; Liquid chromatography ; Mass spectrometry ; Mass spectroscopy ; Middle Aged ; Monitoring/Environmental Analysis ; Phenylethyl Alcohol - analogs & derivatives ; Phenylethyl Alcohol - analysis ; Polymers ; Pretreatment ; Quorum sensing ; Research Paper ; Retention ; Sample preparation ; Sediment samplers ; Sediments ; Selectivity ; Sesquiterpenes ; Shape optimization ; Solid phases ; Solvents ; Sorbents ; Stationary phase ; Tandem Mass Spectrometry - methods ; Tyrosol ; Vagina ; Vagina - chemistry ; Vagina - microbiology ; Young Adult</subject><ispartof>Analytical and bioanalytical chemistry, 2020-09, Vol.412 (24), p.6529-6541</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>COPYRIGHT 2020 Springer</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c545t-67e0caa00e8ae39325f9f905dd66f817135c6f524949083fc366d1df5afe05613</citedby><cites>FETCH-LOGICAL-c545t-67e0caa00e8ae39325f9f905dd66f817135c6f524949083fc366d1df5afe05613</cites><orcidid>0000-0003-3570-5871</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-020-02699-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-020-02699-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32468279$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pilařová, Veronika</creatorcontrib><creatorcontrib>Kočová Vlčková, Hana</creatorcontrib><creatorcontrib>Jung, Ondřej</creatorcontrib><creatorcontrib>Protti, Michele</creatorcontrib><creatorcontrib>Buchta, Vladimír</creatorcontrib><creatorcontrib>Mercolini, Laura</creatorcontrib><creatorcontrib>Svec, Frantisek</creatorcontrib><creatorcontrib>Nováková, Lucie</creatorcontrib><title>Unambiguous determination of farnesol and tyrosol in vaginal fluid using fast and sensitive UHPLC-MS/MS method</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>The new ultra-high performance liquid chromatography method with tandem mass spectrometry detection (UHPLC-MS/MS) has been optimized to allow fast, selective, and high-throughput analysis of two
Candida albicans
quorum sensing molecules (QSM), farnesol and tyrosol. The problem of the presence of the interference in the samples and system was successfully solved by careful optimization of chromatographic conditions. Charged hybrid stationary phase modified with pentafluorophenyl group and optimized gradient elution provided adequate separation selectivity and peak shapes. The impurity was identified as dibutyl phthalate and had the same
m/z
ions as farnesol leading to an important interference on selected reaction monitoring channel. Two different types of biological matrices originating from vaginal fluid, supernatant and sediment, were analysed. Micro-solid phase extraction in pipette tips was optimized for the selective isolation of QSM from the supernatant. The insufficient retention of farnesol on the extraction sorbent was improved when 1% of organic solvent was added prior to extraction, while the retention of tyrosol was only possible when using combined C8 and polymer sorbent type. Strong retention of farnesol had to be solved by increasing elution solvent strength and volume up to 600 μL. However, this approach did not allow the pretreatment of sediment samples due to the sorbent clogging. Therefore, our previously developed protein precipitation method was modified and validated to analyse the sediments. New developed UHPLC-MS/MS method provided suitable accuracy and precision for the determination of QSM in vaginal fluid while using only 50 μL sample volume and two different sample preparation methods.</description><subject>Adult</subject><subject>Alcohols</subject><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Biochemistry</subject><subject>Body fluids</subject><subject>Candida albicans - isolation & purification</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Composition</subject><subject>Dibutyl phthalate</subject><subject>Elution</subject><subject>Farnesol</subject><subject>Farnesol - analysis</subject><subject>Female</subject><subject>Female Role Models in Analytical Chemistry</subject><subject>Food Science</subject><subject>High performance liquid chromatography</subject><subject>Humans</subject><subject>Identification and classification</subject><subject>Interference</subject><subject>Ions</subject><subject>Laboratory Medicine</subject><subject>Limit of Detection</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Middle Aged</subject><subject>Monitoring/Environmental Analysis</subject><subject>Phenylethyl Alcohol - analogs & derivatives</subject><subject>Phenylethyl Alcohol - analysis</subject><subject>Polymers</subject><subject>Pretreatment</subject><subject>Quorum sensing</subject><subject>Research Paper</subject><subject>Retention</subject><subject>Sample preparation</subject><subject>Sediment samplers</subject><subject>Sediments</subject><subject>Selectivity</subject><subject>Sesquiterpenes</subject><subject>Shape optimization</subject><subject>Solid phases</subject><subject>Solvents</subject><subject>Sorbents</subject><subject>Stationary phase</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Tyrosol</subject><subject>Vagina</subject><subject>Vagina - chemistry</subject><subject>Vagina - microbiology</subject><subject>Young Adult</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kV1rFTEQhoMo9kP_gBcS8MabbSfJJrt7WQ7aCqco1HMd0s1kTdlNarJb6L8321NbFJEQMkyed5iZl5B3DE4YQHOaAThTFXAoV3VdxV6QQ6ZYW3El4eVTXPMDcpTzDQCTLVOvyYHgtWp50x2SsAtmuvbDEpdMLc6YJh_M7GOg0VFnUsAcR2qCpfN9imvsA70zQ6FG6sbFW7pkH4bC5vmByxiyn_0d0t3Ft-2murw6vbyiE84_on1DXjkzZnz7-B6T3edP3zcX1fbr-ZfN2bbqZS3nSjUIvTEA2BoUneDSda4Daa1SrmUNE7JXTvK6qztoheuFUpZZJ41DkIqJY_JxX_c2xZ8L5llPPvc4jiZgmVTzGloOjWxVQT_8hd7EJZXpVkrITjSyZs_UYEbUPrg4J9OvRfWZEmWfkrWiUCf_oMqxOPk-BnS-5P8Q8L2gL7vNCZ2-TX4y6V4z0KvJem-yLibrB5P12sv7x46X6wntk-S3qwUQeyCXrzBgeh7pP2V_ATawr7A</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Pilařová, Veronika</creator><creator>Kočová Vlčková, Hana</creator><creator>Jung, Ondřej</creator><creator>Protti, Michele</creator><creator>Buchta, Vladimír</creator><creator>Mercolini, Laura</creator><creator>Svec, Frantisek</creator><creator>Nováková, Lucie</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3570-5871</orcidid></search><sort><creationdate>20200901</creationdate><title>Unambiguous determination of farnesol and tyrosol in vaginal fluid using fast and sensitive UHPLC-MS/MS method</title><author>Pilařová, Veronika ; Kočová Vlčková, Hana ; Jung, Ondřej ; Protti, Michele ; Buchta, Vladimír ; Mercolini, Laura ; Svec, Frantisek ; Nováková, Lucie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c545t-67e0caa00e8ae39325f9f905dd66f817135c6f524949083fc366d1df5afe05613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adult</topic><topic>Alcohols</topic><topic>Analysis</topic><topic>Analytical Chemistry</topic><topic>Biochemistry</topic><topic>Body fluids</topic><topic>Candida albicans - isolation & purification</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Composition</topic><topic>Dibutyl phthalate</topic><topic>Elution</topic><topic>Farnesol</topic><topic>Farnesol - analysis</topic><topic>Female</topic><topic>Female Role Models in Analytical Chemistry</topic><topic>Food Science</topic><topic>High performance liquid chromatography</topic><topic>Humans</topic><topic>Identification and classification</topic><topic>Interference</topic><topic>Ions</topic><topic>Laboratory Medicine</topic><topic>Limit of Detection</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Middle Aged</topic><topic>Monitoring/Environmental Analysis</topic><topic>Phenylethyl Alcohol - analogs & derivatives</topic><topic>Phenylethyl Alcohol - analysis</topic><topic>Polymers</topic><topic>Pretreatment</topic><topic>Quorum sensing</topic><topic>Research Paper</topic><topic>Retention</topic><topic>Sample preparation</topic><topic>Sediment samplers</topic><topic>Sediments</topic><topic>Selectivity</topic><topic>Sesquiterpenes</topic><topic>Shape optimization</topic><topic>Solid phases</topic><topic>Solvents</topic><topic>Sorbents</topic><topic>Stationary phase</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Tyrosol</topic><topic>Vagina</topic><topic>Vagina - chemistry</topic><topic>Vagina - microbiology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pilařová, Veronika</creatorcontrib><creatorcontrib>Kočová Vlčková, Hana</creatorcontrib><creatorcontrib>Jung, Ondřej</creatorcontrib><creatorcontrib>Protti, Michele</creatorcontrib><creatorcontrib>Buchta, Vladimír</creatorcontrib><creatorcontrib>Mercolini, Laura</creatorcontrib><creatorcontrib>Svec, Frantisek</creatorcontrib><creatorcontrib>Nováková, Lucie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pilařová, Veronika</au><au>Kočová Vlčková, Hana</au><au>Jung, Ondřej</au><au>Protti, Michele</au><au>Buchta, Vladimír</au><au>Mercolini, Laura</au><au>Svec, Frantisek</au><au>Nováková, Lucie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Unambiguous determination of farnesol and tyrosol in vaginal fluid using fast and sensitive UHPLC-MS/MS method</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2020-09-01</date><risdate>2020</risdate><volume>412</volume><issue>24</issue><spage>6529</spage><epage>6541</epage><pages>6529-6541</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>The new ultra-high performance liquid chromatography method with tandem mass spectrometry detection (UHPLC-MS/MS) has been optimized to allow fast, selective, and high-throughput analysis of two
Candida albicans
quorum sensing molecules (QSM), farnesol and tyrosol. The problem of the presence of the interference in the samples and system was successfully solved by careful optimization of chromatographic conditions. Charged hybrid stationary phase modified with pentafluorophenyl group and optimized gradient elution provided adequate separation selectivity and peak shapes. The impurity was identified as dibutyl phthalate and had the same
m/z
ions as farnesol leading to an important interference on selected reaction monitoring channel. Two different types of biological matrices originating from vaginal fluid, supernatant and sediment, were analysed. Micro-solid phase extraction in pipette tips was optimized for the selective isolation of QSM from the supernatant. The insufficient retention of farnesol on the extraction sorbent was improved when 1% of organic solvent was added prior to extraction, while the retention of tyrosol was only possible when using combined C8 and polymer sorbent type. Strong retention of farnesol had to be solved by increasing elution solvent strength and volume up to 600 μL. However, this approach did not allow the pretreatment of sediment samples due to the sorbent clogging. Therefore, our previously developed protein precipitation method was modified and validated to analyse the sediments. New developed UHPLC-MS/MS method provided suitable accuracy and precision for the determination of QSM in vaginal fluid while using only 50 μL sample volume and two different sample preparation methods.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32468279</pmid><doi>10.1007/s00216-020-02699-1</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-3570-5871</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1618-2642 |
| ispartof | Analytical and bioanalytical chemistry, 2020-09, Vol.412 (24), p.6529-6541 |
| issn | 1618-2642 1618-2650 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2408207586 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Adult Alcohols Analysis Analytical Chemistry Biochemistry Body fluids Candida albicans - isolation & purification Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Chromatography, High Pressure Liquid - methods Composition Dibutyl phthalate Elution Farnesol Farnesol - analysis Female Female Role Models in Analytical Chemistry Food Science High performance liquid chromatography Humans Identification and classification Interference Ions Laboratory Medicine Limit of Detection Liquid chromatography Mass spectrometry Mass spectroscopy Middle Aged Monitoring/Environmental Analysis Phenylethyl Alcohol - analogs & derivatives Phenylethyl Alcohol - analysis Polymers Pretreatment Quorum sensing Research Paper Retention Sample preparation Sediment samplers Sediments Selectivity Sesquiterpenes Shape optimization Solid phases Solvents Sorbents Stationary phase Tandem Mass Spectrometry - methods Tyrosol Vagina Vagina - chemistry Vagina - microbiology Young Adult |
| title | Unambiguous determination of farnesol and tyrosol in vaginal fluid using fast and sensitive UHPLC-MS/MS method |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T17%3A03%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Unambiguous%20determination%20of%20farnesol%20and%20tyrosol%20in%20vaginal%20fluid%20using%20fast%20and%20sensitive%20UHPLC-MS/MS%20method&rft.jtitle=Analytical%20and%20bioanalytical%20chemistry&rft.au=Pila%C5%99ov%C3%A1,%20Veronika&rft.date=2020-09-01&rft.volume=412&rft.issue=24&rft.spage=6529&rft.epage=6541&rft.pages=6529-6541&rft.issn=1618-2642&rft.eissn=1618-2650&rft_id=info:doi/10.1007/s00216-020-02699-1&rft_dat=%3Cgale_proqu%3EA633245183%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2435937541&rft_id=info:pmid/32468279&rft_galeid=A633245183&rfr_iscdi=true |