Long intergenic non-coding RNA-p21 is associated with poor prognosis in chronic lymphocytic leukemia
Background Long non-coding RNAs (LncRNAs) are RNA transcripts longer than 200 nucleotides. They are new players in transcriptional regulation and cancer research. LincRNA-p21 is a p53-regulated lncRNA involved in the p53 transcriptional network. It has an important role in regulating cellular prolif...
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| Veröffentlicht in: | Clinical & translational oncology 2021, Vol.23 (1), p.92-99 |
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| creator | Abo Elwafa, R. Abd Elrahman, A. Ghallab, O. |
| description | Background
Long non-coding RNAs (LncRNAs) are RNA transcripts longer than 200 nucleotides. They are new players in transcriptional regulation and cancer research. LincRNA-p21 is a p53-regulated lncRNA involved in the p53 transcriptional network. It has an important role in regulating cellular proliferation and apoptosis. Chronic lymphocytic leukemia is derived by a typical defect in apoptosis and characterized by clonal proliferation and accumulation of mature B cells. The aim of the present study was to assess the expression pattern of the lincRNA-p21 and investigate its potential role as a new prognostic marker in CLL.
Methods
The study was conducted on 80 newly diagnosed CLL patients and 80 age- and sex-matched controls. The analysis of LincRNA-p21 and the p53 downstream proapoptotic target genes (MDM2, PUMA, BAX, and NOXA) was performed by real-time PCR. The cytogenetic abrasions and expression of ZAP70 and CD38 were detected by FISH and Flow cytometry, respectively.
Results
LincRNA-p21 was significantly downregulated in CLL patients compared to controls. The downstream proapoptotic targets were significantly downregulated in CLL patients and positively correlated with lincRNA-p21. Low expression of lincRNA-p21 was associated with poor prognostic markers (advanced stages of CLL, del 17p13, ZAP70, and CD38 expression), failure of complete remission, shorter progression free survival, and overall survival. Low lincRNA-p21 expression was independently prognostic for shorter time to treatment.
Conclusion
Low expression of lincRNA-p21 demarcates a more aggressive form of CLL with poor prognosis. Therefore, it could be considered as a new prognostic marker to predict disease outcome in CLL. |
| doi_str_mv | 10.1007/s12094-020-02398-4 |
| format | Article |
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Long non-coding RNAs (LncRNAs) are RNA transcripts longer than 200 nucleotides. They are new players in transcriptional regulation and cancer research. LincRNA-p21 is a p53-regulated lncRNA involved in the p53 transcriptional network. It has an important role in regulating cellular proliferation and apoptosis. Chronic lymphocytic leukemia is derived by a typical defect in apoptosis and characterized by clonal proliferation and accumulation of mature B cells. The aim of the present study was to assess the expression pattern of the lincRNA-p21 and investigate its potential role as a new prognostic marker in CLL.
Methods
The study was conducted on 80 newly diagnosed CLL patients and 80 age- and sex-matched controls. The analysis of LincRNA-p21 and the p53 downstream proapoptotic target genes (MDM2, PUMA, BAX, and NOXA) was performed by real-time PCR. The cytogenetic abrasions and expression of ZAP70 and CD38 were detected by FISH and Flow cytometry, respectively.
Results
LincRNA-p21 was significantly downregulated in CLL patients compared to controls. The downstream proapoptotic targets were significantly downregulated in CLL patients and positively correlated with lincRNA-p21. Low expression of lincRNA-p21 was associated with poor prognostic markers (advanced stages of CLL, del 17p13, ZAP70, and CD38 expression), failure of complete remission, shorter progression free survival, and overall survival. Low lincRNA-p21 expression was independently prognostic for shorter time to treatment.
Conclusion
Low expression of lincRNA-p21 demarcates a more aggressive form of CLL with poor prognosis. Therefore, it could be considered as a new prognostic marker to predict disease outcome in CLL.</description><identifier>ISSN: 1699-048X</identifier><identifier>EISSN: 1699-3055</identifier><identifier>DOI: 10.1007/s12094-020-02398-4</identifier><identifier>PMID: 32468342</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Medicine ; Medicine & Public Health ; Oncology ; Research Article</subject><ispartof>Clinical & translational oncology, 2021, Vol.23 (1), p.92-99</ispartof><rights>Federación de Sociedades Españolas de Oncología (FESEO) 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c347t-7924bc6cbecdaf470f3430f73892c5f4555be7844612507ea657fa768a1b03e03</citedby><cites>FETCH-LOGICAL-c347t-7924bc6cbecdaf470f3430f73892c5f4555be7844612507ea657fa768a1b03e03</cites><orcidid>0000-0003-2113-3569</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12094-020-02398-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12094-020-02398-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32468342$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abo Elwafa, R.</creatorcontrib><creatorcontrib>Abd Elrahman, A.</creatorcontrib><creatorcontrib>Ghallab, O.</creatorcontrib><title>Long intergenic non-coding RNA-p21 is associated with poor prognosis in chronic lymphocytic leukemia</title><title>Clinical & translational oncology</title><addtitle>Clin Transl Oncol</addtitle><addtitle>Clin Transl Oncol</addtitle><description>Background
Long non-coding RNAs (LncRNAs) are RNA transcripts longer than 200 nucleotides. They are new players in transcriptional regulation and cancer research. LincRNA-p21 is a p53-regulated lncRNA involved in the p53 transcriptional network. It has an important role in regulating cellular proliferation and apoptosis. Chronic lymphocytic leukemia is derived by a typical defect in apoptosis and characterized by clonal proliferation and accumulation of mature B cells. The aim of the present study was to assess the expression pattern of the lincRNA-p21 and investigate its potential role as a new prognostic marker in CLL.
Methods
The study was conducted on 80 newly diagnosed CLL patients and 80 age- and sex-matched controls. The analysis of LincRNA-p21 and the p53 downstream proapoptotic target genes (MDM2, PUMA, BAX, and NOXA) was performed by real-time PCR. The cytogenetic abrasions and expression of ZAP70 and CD38 were detected by FISH and Flow cytometry, respectively.
Results
LincRNA-p21 was significantly downregulated in CLL patients compared to controls. The downstream proapoptotic targets were significantly downregulated in CLL patients and positively correlated with lincRNA-p21. Low expression of lincRNA-p21 was associated with poor prognostic markers (advanced stages of CLL, del 17p13, ZAP70, and CD38 expression), failure of complete remission, shorter progression free survival, and overall survival. Low lincRNA-p21 expression was independently prognostic for shorter time to treatment.
Conclusion
Low expression of lincRNA-p21 demarcates a more aggressive form of CLL with poor prognosis. Therefore, it could be considered as a new prognostic marker to predict disease outcome in CLL.</description><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Oncology</subject><subject>Research Article</subject><issn>1699-048X</issn><issn>1699-3055</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAQhoMorq7-AQ_So5fq5KtpjyJ-waIgCt5Cmqa7WbdJTVpk_71Zd_XoYZhh5n1fkgehMwyXGEBcRUygYjkQSEWrMmd76AgXVZVT4Hx_NwMr3yfoOMYlpG2B8SGaUMKKkjJyhJqZd_PMusGEuXFWZ867XPvGpu3L03XeE5zZmKkYvbZqME32ZYdF1nsfsj74ufMxna3L9CL4jX-17vqF1-thM5vxw3RWnaCDVq2iOd31KXq7u329echnz_ePN9ezXFMmhlxUhNW60LXRjWqZgJYyCq2gZUU0bxnnvDaiZKzAhIMwquCiVaIoFa6BGqBTdLHNTS_7HE0cZGejNquVcsaPURIGJQFOWZGkZCvVwccYTCv7YDsV1hKD3NCVW7oy0ZU_dCVLpvNd_lh3pvmz_OJMAroVxHRycxPk0o_BpT__F_sNvXyFfg</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Abo Elwafa, R.</creator><creator>Abd Elrahman, A.</creator><creator>Ghallab, O.</creator><general>Springer International Publishing</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2113-3569</orcidid></search><sort><creationdate>2021</creationdate><title>Long intergenic non-coding RNA-p21 is associated with poor prognosis in chronic lymphocytic leukemia</title><author>Abo Elwafa, R. ; Abd Elrahman, A. ; Ghallab, O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-7924bc6cbecdaf470f3430f73892c5f4555be7844612507ea657fa768a1b03e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Oncology</topic><topic>Research Article</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abo Elwafa, R.</creatorcontrib><creatorcontrib>Abd Elrahman, A.</creatorcontrib><creatorcontrib>Ghallab, O.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical & translational oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abo Elwafa, R.</au><au>Abd Elrahman, A.</au><au>Ghallab, O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Long intergenic non-coding RNA-p21 is associated with poor prognosis in chronic lymphocytic leukemia</atitle><jtitle>Clinical & translational oncology</jtitle><stitle>Clin Transl Oncol</stitle><addtitle>Clin Transl Oncol</addtitle><date>2021</date><risdate>2021</risdate><volume>23</volume><issue>1</issue><spage>92</spage><epage>99</epage><pages>92-99</pages><issn>1699-048X</issn><eissn>1699-3055</eissn><abstract>Background
Long non-coding RNAs (LncRNAs) are RNA transcripts longer than 200 nucleotides. They are new players in transcriptional regulation and cancer research. LincRNA-p21 is a p53-regulated lncRNA involved in the p53 transcriptional network. It has an important role in regulating cellular proliferation and apoptosis. Chronic lymphocytic leukemia is derived by a typical defect in apoptosis and characterized by clonal proliferation and accumulation of mature B cells. The aim of the present study was to assess the expression pattern of the lincRNA-p21 and investigate its potential role as a new prognostic marker in CLL.
Methods
The study was conducted on 80 newly diagnosed CLL patients and 80 age- and sex-matched controls. The analysis of LincRNA-p21 and the p53 downstream proapoptotic target genes (MDM2, PUMA, BAX, and NOXA) was performed by real-time PCR. The cytogenetic abrasions and expression of ZAP70 and CD38 were detected by FISH and Flow cytometry, respectively.
Results
LincRNA-p21 was significantly downregulated in CLL patients compared to controls. The downstream proapoptotic targets were significantly downregulated in CLL patients and positively correlated with lincRNA-p21. Low expression of lincRNA-p21 was associated with poor prognostic markers (advanced stages of CLL, del 17p13, ZAP70, and CD38 expression), failure of complete remission, shorter progression free survival, and overall survival. Low lincRNA-p21 expression was independently prognostic for shorter time to treatment.
Conclusion
Low expression of lincRNA-p21 demarcates a more aggressive form of CLL with poor prognosis. Therefore, it could be considered as a new prognostic marker to predict disease outcome in CLL.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>32468342</pmid><doi>10.1007/s12094-020-02398-4</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-2113-3569</orcidid></addata></record> |
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| source | SpringerNature Journals |
| subjects | Medicine Medicine & Public Health Oncology Research Article |
| title | Long intergenic non-coding RNA-p21 is associated with poor prognosis in chronic lymphocytic leukemia |
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