A suitable and effective stepwise oxidative refolding procedure for highly‐cationic tetrameric avidin in nucleic acid free conditions

Optimized conditions are needed to refold recombinant proteins from bacterial inclusion bodies into their biologically active conformations. In this study, we found two crucial requirements for efficient refolding of cationic tetrameric chicken avidin. The first step is to eliminate nucleic acid con...

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Veröffentlicht in:	Biotechnology progress 2020-09, Vol.36 (5), p.e3031-n/a
Hauptverfasser:	Kimura, Shuichiro, Imamura, Koreyoshi, Futami, Junichiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Avidin Biological activity Cations chemical modification Contaminants Dialysis Electrostatic properties Inclusion bodies Nucleic acids Poultry Protein folding Protein interaction Protein purification protein refolding Proteins
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