Teaching old indicators even more tricks: binding affinity measurements with the guest-displacement assay (GDA)

Teaching old indicators even more tricks: binding affinity measurements with the guest-displacement assay (GDA)

A simple change has important consequences: the guest-displacement assay (GDA) is introduced which allows for binding affinity determinations of supramolecular complexes with spectroscopically silent hosts and guests. GDA is complementary to indicator-displacement assay for affinity measurements wit...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2020-06, Vol.56 (49), p.662-6623
Hauptverfasser: Sinn, Stephan, Krämer, Joana, Biedermann, Frank
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Sprache:eng
Schlagworte:
Affinity
Assaying
Binding
Displacement
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container_title Chemical communications (Cambridge, England)
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creator Sinn, Stephan
Krämer, Joana
Biedermann, Frank
description A simple change has important consequences: the guest-displacement assay (GDA) is introduced which allows for binding affinity determinations of supramolecular complexes with spectroscopically silent hosts and guests. GDA is complementary to indicator-displacement assay for affinity measurements with soluble components, but is superior for insoluble or for weakly binding guests. A simple change has important consequences: the guest-displacement assay (GDA) is introduced which allows for binding affinity determinations of supramolecular complexes with spectroscopically silent hosts and guests.
doi_str_mv 10.1039/d0cc01841d
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source Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection
subjects Affinity
Assaying
Binding
Displacement
title Teaching old indicators even more tricks: binding affinity measurements with the guest-displacement assay (GDA)
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