Global priority multidrug-resistant pathogens do not resist photodynamic therapy
Microbial drug-resistance demands immediate implementation of novel therapeutic strategies. Antimicrobial photodynamic therapy (aPDT) combines the administration of a photosensitizer (PS) compound with low-irradiance light to induce photochemical reactions that yield reactive oxygen species (ROS). S...
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| Veröffentlicht in: | Journal of photochemistry and photobiology. B, Biology Biology, 2020-07, Vol.208, p.111893-111893, Article 111893 |
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| container_title | Journal of photochemistry and photobiology. B, Biology |
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| creator | Sabino, Caetano Padial Wainwright, Mark Ribeiro, Martha Simões Sellera, Fábio Parra dos Anjos, Carolina Baptista, Mauricio da Silva Lincopan, Nilton |
| description | Microbial drug-resistance demands immediate implementation of novel therapeutic strategies. Antimicrobial photodynamic therapy (aPDT) combines the administration of a photosensitizer (PS) compound with low-irradiance light to induce photochemical reactions that yield reactive oxygen species (ROS). Since ROS react with nearly all biomolecules, aPDT offers a powerful multitarget method to avoid selection of drug-resistant strains. In this study, we assayed photodynamic inactivation under a standardized method, combining methylene blue (MB) as PS and red light, against global priority pathogens. The species tested include Acinetobacter baumannii, Klebsiella aerogenes, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus, Candida albicans and Cryptococcus neoformans. Our strain collection presents resistance to all tested antimicrobials (>50). All drug-resistant strains were compared to their drug-sensitive counterparts. Regardless of resistance phenotype, MB-aPDT presented species-specific dose-response kinetics. More than 5log10 reduction was observed within less than 75 s of illumination for A. baumannii, E. coli, E. faecium, E. faecalis and S. aureus and within less than 7 min for K. aerogenes, K. pneumoniae, P. aeruginosa, C. albicans and C. neoformans. No signs of correlations in between drug-resistance profiles and aPDT sensitivity were observed. Therefore, MB-aPDT can provide effective therapeutic protocols for a very broad spectrum of pathogens. Hence, we believe that this study represents a very important step to bring aPDT closer to implementation into mainstream medical practices.
•APDT was assayed against the top global priority drug-resistant pathogens•We tested 27 strains from 10 pathogen species under a standard method•Our strain collection presented resistance to more than 50 antimicrobials•No signs of cross-resistance in between APDT and chemotherapy was observed•We inactivated more than 99.999% of any strain within less than 7 min |
| doi_str_mv | 10.1016/j.jphotobiol.2020.111893 |
| format | Article |
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•APDT was assayed against the top global priority drug-resistant pathogens•We tested 27 strains from 10 pathogen species under a standard method•Our strain collection presented resistance to more than 50 antimicrobials•No signs of cross-resistance in between APDT and chemotherapy was observed•We inactivated more than 99.999% of any strain within less than 7 min</description><identifier>ISSN: 1011-1344</identifier><identifier>EISSN: 1873-2682</identifier><identifier>DOI: 10.1016/j.jphotobiol.2020.111893</identifier><identifier>PMID: 32446039</identifier><language>eng</language><publisher>Switzerland: Elsevier B.V</publisher><subject>Aerogenes ; Antiinfectives and antibacterials ; Antimicrobial agents ; Bacteria ; Biomolecules ; Candida albicans - drug effects ; Cryptococcus neoformans ; Deactivation ; Drug resistance ; Drug Resistance, Microbial - drug effects ; E coli ; ESKAPE ; Fungi ; Gram-Negative Bacteria - drug effects ; Gram-Positive Bacteria - drug effects ; Inactivation ; Irradiance ; Kinetics ; Klebsiella ; Klebsiella pneumoniae ; Light ; Methylene blue ; Methylene Blue - pharmacology ; Microbial Sensitivity Tests ; Microorganisms ; Multidrug resistance ; Pathogens ; Phenotypes ; Photochemical reactions ; Photochemicals ; Photodynamic therapy ; Photoinactivation ; Photosensitizing Agents - pharmacology ; Pseudomonas aeruginosa ; Reaction kinetics ; Reactive oxygen species</subject><ispartof>Journal of photochemistry and photobiology. B, Biology, 2020-07, Vol.208, p.111893-111893, Article 111893</ispartof><rights>2020 Elsevier B.V.</rights><rights>Copyright © 2020 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV Jul 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-b08b93aafbc2d05d56c8104f76e306a8dd49c226d14c449a8ec064a5201784153</citedby><cites>FETCH-LOGICAL-c452t-b08b93aafbc2d05d56c8104f76e306a8dd49c226d14c449a8ec064a5201784153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jphotobiol.2020.111893$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32446039$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sabino, Caetano Padial</creatorcontrib><creatorcontrib>Wainwright, Mark</creatorcontrib><creatorcontrib>Ribeiro, Martha Simões</creatorcontrib><creatorcontrib>Sellera, Fábio Parra</creatorcontrib><creatorcontrib>dos Anjos, Carolina</creatorcontrib><creatorcontrib>Baptista, Mauricio da Silva</creatorcontrib><creatorcontrib>Lincopan, Nilton</creatorcontrib><title>Global priority multidrug-resistant pathogens do not resist photodynamic therapy</title><title>Journal of photochemistry and photobiology. B, Biology</title><addtitle>J Photochem Photobiol B</addtitle><description>Microbial drug-resistance demands immediate implementation of novel therapeutic strategies. Antimicrobial photodynamic therapy (aPDT) combines the administration of a photosensitizer (PS) compound with low-irradiance light to induce photochemical reactions that yield reactive oxygen species (ROS). Since ROS react with nearly all biomolecules, aPDT offers a powerful multitarget method to avoid selection of drug-resistant strains. In this study, we assayed photodynamic inactivation under a standardized method, combining methylene blue (MB) as PS and red light, against global priority pathogens. The species tested include Acinetobacter baumannii, Klebsiella aerogenes, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus, Candida albicans and Cryptococcus neoformans. Our strain collection presents resistance to all tested antimicrobials (>50). All drug-resistant strains were compared to their drug-sensitive counterparts. Regardless of resistance phenotype, MB-aPDT presented species-specific dose-response kinetics. More than 5log10 reduction was observed within less than 75 s of illumination for A. baumannii, E. coli, E. faecium, E. faecalis and S. aureus and within less than 7 min for K. aerogenes, K. pneumoniae, P. aeruginosa, C. albicans and C. neoformans. No signs of correlations in between drug-resistance profiles and aPDT sensitivity were observed. Therefore, MB-aPDT can provide effective therapeutic protocols for a very broad spectrum of pathogens. Hence, we believe that this study represents a very important step to bring aPDT closer to implementation into mainstream medical practices.
•APDT was assayed against the top global priority drug-resistant pathogens•We tested 27 strains from 10 pathogen species under a standard method•Our strain collection presented resistance to more than 50 antimicrobials•No signs of cross-resistance in between APDT and chemotherapy was observed•We inactivated more than 99.999% of any strain within less than 7 min</description><subject>Aerogenes</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Biomolecules</subject><subject>Candida albicans - drug effects</subject><subject>Cryptococcus neoformans</subject><subject>Deactivation</subject><subject>Drug resistance</subject><subject>Drug Resistance, Microbial - drug effects</subject><subject>E coli</subject><subject>ESKAPE</subject><subject>Fungi</subject><subject>Gram-Negative Bacteria - drug effects</subject><subject>Gram-Positive Bacteria - drug effects</subject><subject>Inactivation</subject><subject>Irradiance</subject><subject>Kinetics</subject><subject>Klebsiella</subject><subject>Klebsiella pneumoniae</subject><subject>Light</subject><subject>Methylene blue</subject><subject>Methylene Blue - pharmacology</subject><subject>Microbial Sensitivity Tests</subject><subject>Microorganisms</subject><subject>Multidrug resistance</subject><subject>Pathogens</subject><subject>Phenotypes</subject><subject>Photochemical reactions</subject><subject>Photochemicals</subject><subject>Photodynamic therapy</subject><subject>Photoinactivation</subject><subject>Photosensitizing Agents - pharmacology</subject><subject>Pseudomonas aeruginosa</subject><subject>Reaction kinetics</subject><subject>Reactive oxygen species</subject><issn>1011-1344</issn><issn>1873-2682</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOxCAUhonReH8FQ-LGTUdupXSpxltiogtdEwqMQ9OWCtRk3l7GekncyAZy-M75Tz4AIEYLjDA_bxftuPLJN853C4JILmMsaroF9rGoaEG4INv5jTAuMGVsDxzE2KJ8Sl7tgj1KGOOI1vvg6bbzjergGJwPLq1hP3XJmTC9FsFGF5MaEhxVWvlXO0RoPBx8gvMX_NzBrAfVOw3TygY1ro_AzlJ10R5_3Yfg5eb6-equeHi8vb-6eCg0K0kqGiSamiq1bDQxqDQl1wIjtqy4pYgrYQyrNSHcYKYZq5WwGnGmSoJwJRgu6SE4m-eOwb9NNibZu6ht16nB-ilKwhCnqBKEZ_T0D9r6KQx5u0wxwnFNyw0lZkoHH2OwS5md9CqsJUZyY1228te63FiXs_XcevIVMDW9NT-N35ozcDkDNht5dzbIqJ0dtDUuWJ2k8e7_lA_fZ5in</recordid><startdate>202007</startdate><enddate>202007</enddate><creator>Sabino, Caetano Padial</creator><creator>Wainwright, Mark</creator><creator>Ribeiro, Martha Simões</creator><creator>Sellera, Fábio Parra</creator><creator>dos Anjos, Carolina</creator><creator>Baptista, Mauricio da Silva</creator><creator>Lincopan, Nilton</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>202007</creationdate><title>Global priority multidrug-resistant pathogens do not resist photodynamic therapy</title><author>Sabino, Caetano Padial ; 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B, Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sabino, Caetano Padial</au><au>Wainwright, Mark</au><au>Ribeiro, Martha Simões</au><au>Sellera, Fábio Parra</au><au>dos Anjos, Carolina</au><au>Baptista, Mauricio da Silva</au><au>Lincopan, Nilton</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Global priority multidrug-resistant pathogens do not resist photodynamic therapy</atitle><jtitle>Journal of photochemistry and photobiology. B, Biology</jtitle><addtitle>J Photochem Photobiol B</addtitle><date>2020-07</date><risdate>2020</risdate><volume>208</volume><spage>111893</spage><epage>111893</epage><pages>111893-111893</pages><artnum>111893</artnum><issn>1011-1344</issn><eissn>1873-2682</eissn><abstract>Microbial drug-resistance demands immediate implementation of novel therapeutic strategies. Antimicrobial photodynamic therapy (aPDT) combines the administration of a photosensitizer (PS) compound with low-irradiance light to induce photochemical reactions that yield reactive oxygen species (ROS). Since ROS react with nearly all biomolecules, aPDT offers a powerful multitarget method to avoid selection of drug-resistant strains. In this study, we assayed photodynamic inactivation under a standardized method, combining methylene blue (MB) as PS and red light, against global priority pathogens. The species tested include Acinetobacter baumannii, Klebsiella aerogenes, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus, Candida albicans and Cryptococcus neoformans. Our strain collection presents resistance to all tested antimicrobials (>50). All drug-resistant strains were compared to their drug-sensitive counterparts. Regardless of resistance phenotype, MB-aPDT presented species-specific dose-response kinetics. More than 5log10 reduction was observed within less than 75 s of illumination for A. baumannii, E. coli, E. faecium, E. faecalis and S. aureus and within less than 7 min for K. aerogenes, K. pneumoniae, P. aeruginosa, C. albicans and C. neoformans. No signs of correlations in between drug-resistance profiles and aPDT sensitivity were observed. Therefore, MB-aPDT can provide effective therapeutic protocols for a very broad spectrum of pathogens. Hence, we believe that this study represents a very important step to bring aPDT closer to implementation into mainstream medical practices.
•APDT was assayed against the top global priority drug-resistant pathogens•We tested 27 strains from 10 pathogen species under a standard method•Our strain collection presented resistance to more than 50 antimicrobials•No signs of cross-resistance in between APDT and chemotherapy was observed•We inactivated more than 99.999% of any strain within less than 7 min</abstract><cop>Switzerland</cop><pub>Elsevier B.V</pub><pmid>32446039</pmid><doi>10.1016/j.jphotobiol.2020.111893</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aerogenes Antiinfectives and antibacterials Antimicrobial agents Bacteria Biomolecules Candida albicans - drug effects Cryptococcus neoformans Deactivation Drug resistance Drug Resistance, Microbial - drug effects E coli ESKAPE Fungi Gram-Negative Bacteria - drug effects Gram-Positive Bacteria - drug effects Inactivation Irradiance Kinetics Klebsiella Klebsiella pneumoniae Light Methylene blue Methylene Blue - pharmacology Microbial Sensitivity Tests Microorganisms Multidrug resistance Pathogens Phenotypes Photochemical reactions Photochemicals Photodynamic therapy Photoinactivation Photosensitizing Agents - pharmacology Pseudomonas aeruginosa Reaction kinetics Reactive oxygen species |
| title | Global priority multidrug-resistant pathogens do not resist photodynamic therapy |
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