Absence of damaging effects of stem cell donation in unrelated donors assessed by FISH and gene variance screening
Astract Granulocyte–Colony-Stimulating factor (G-CSF) is currently the standard mobilising agent for peripheral blood stem cell (PBSC) donation. Concerns that it may trigger chromosome aberrations similar to those observed in leukaemia patients were refuted but long-term effects of G-CSF mobilisatio...
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| Veröffentlicht in: | Bone marrow transplantation (Basingstoke) 2020-07, Vol.55 (7), p.1290-1296 |
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| creator | Nacheva, E. Ahyee, T. Addada, J. Navarette, C. Pamphilon, D. Regan, F. Pawson, R. Szydlo, R. Goldman, J. Mackinnon, S. Shaw, B. E. Madrigal, A. |
| description | Astract
Granulocyte–Colony-Stimulating factor (G-CSF) is currently the standard mobilising agent for peripheral blood stem cell (PBSC) donation. Concerns that it may trigger chromosome aberrations similar to those observed in leukaemia patients were refuted but long-term effects of G-CSF mobilisation on genome integrity remains unclear. In the setting of a multi-centre clinical trial we screened blood samples from 50 PBSC donors at cellular and gene level for aberrations common in haematological malignancies using fluorescence in situ hybridisation (FISH) and next generation sequencing (NGS) assays. Analysis of samples collected before, on the day of donation, 90 and 180 days after G-CSF admission confirmed the absence of short-term effects in PBSC donors on both quiescent and dividing cells. This data did not differ from the results of 50 individuals tested 3–5 years after bone marrow donation and 50 healthy persons. NGS using a panel targeting 54 genes recurrently affected in myeloid disorders (
TruSight Myeloid panel, Illumina
) showed that the gene profiles of samples from 48 PBSC donors remained stable throughout the study period. These data strongly indicate absence of detrimental effects on the genome integrity caused by PBSC donation. |
| doi_str_mv | 10.1038/s41409-020-0945-y |
| format | Article |
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Granulocyte–Colony-Stimulating factor (G-CSF) is currently the standard mobilising agent for peripheral blood stem cell (PBSC) donation. Concerns that it may trigger chromosome aberrations similar to those observed in leukaemia patients were refuted but long-term effects of G-CSF mobilisation on genome integrity remains unclear. In the setting of a multi-centre clinical trial we screened blood samples from 50 PBSC donors at cellular and gene level for aberrations common in haematological malignancies using fluorescence in situ hybridisation (FISH) and next generation sequencing (NGS) assays. Analysis of samples collected before, on the day of donation, 90 and 180 days after G-CSF admission confirmed the absence of short-term effects in PBSC donors on both quiescent and dividing cells. This data did not differ from the results of 50 individuals tested 3–5 years after bone marrow donation and 50 healthy persons. NGS using a panel targeting 54 genes recurrently affected in myeloid disorders (
TruSight Myeloid panel, Illumina
) showed that the gene profiles of samples from 48 PBSC donors remained stable throughout the study period. These data strongly indicate absence of detrimental effects on the genome integrity caused by PBSC donation.</description><identifier>ISSN: 0268-3369</identifier><identifier>EISSN: 1476-5365</identifier><identifier>DOI: 10.1038/s41409-020-0945-y</identifier><identifier>PMID: 32440014</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>14/32 ; 45/23 ; 631/208/212 ; 631/532 ; Blood ; Blood cancer ; Bone marrow ; Cell Biology ; Chromosome aberrations ; Chromosomes ; Colony-stimulating factor ; Fluorescence ; Fluorescence in situ hybridization ; Genomes ; Granulocyte colony-stimulating factor ; Hematology ; Hematopoietic stem cells ; Integrity ; Internal Medicine ; Leukemia ; Leukocytes (granulocytic) ; Long-term effects ; Medicine ; Medicine & Public Health ; Next-generation sequencing ; Peripheral blood ; Public Health ; Stem cell transplantation ; Stem Cells</subject><ispartof>Bone marrow transplantation (Basingstoke), 2020-07, Vol.55 (7), p.1290-1296</ispartof><rights>The Author(s), under exclusive licence to Springer Nature Limited 2020</rights><rights>The Author(s), under exclusive licence to Springer Nature Limited 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c352t-96d3d49373e12337d8aef6ef181d940920fcf75501a04b7217f9f42dd25627b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/s41409-020-0945-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/s41409-020-0945-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32440014$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nacheva, E.</creatorcontrib><creatorcontrib>Ahyee, T.</creatorcontrib><creatorcontrib>Addada, J.</creatorcontrib><creatorcontrib>Navarette, C.</creatorcontrib><creatorcontrib>Pamphilon, D.</creatorcontrib><creatorcontrib>Regan, F.</creatorcontrib><creatorcontrib>Pawson, R.</creatorcontrib><creatorcontrib>Szydlo, R.</creatorcontrib><creatorcontrib>Goldman, J.</creatorcontrib><creatorcontrib>Mackinnon, S.</creatorcontrib><creatorcontrib>Shaw, B. E.</creatorcontrib><creatorcontrib>Madrigal, A.</creatorcontrib><title>Absence of damaging effects of stem cell donation in unrelated donors assessed by FISH and gene variance screening</title><title>Bone marrow transplantation (Basingstoke)</title><addtitle>Bone Marrow Transplant</addtitle><addtitle>Bone Marrow Transplant</addtitle><description>Astract
Granulocyte–Colony-Stimulating factor (G-CSF) is currently the standard mobilising agent for peripheral blood stem cell (PBSC) donation. Concerns that it may trigger chromosome aberrations similar to those observed in leukaemia patients were refuted but long-term effects of G-CSF mobilisation on genome integrity remains unclear. In the setting of a multi-centre clinical trial we screened blood samples from 50 PBSC donors at cellular and gene level for aberrations common in haematological malignancies using fluorescence in situ hybridisation (FISH) and next generation sequencing (NGS) assays. Analysis of samples collected before, on the day of donation, 90 and 180 days after G-CSF admission confirmed the absence of short-term effects in PBSC donors on both quiescent and dividing cells. This data did not differ from the results of 50 individuals tested 3–5 years after bone marrow donation and 50 healthy persons. NGS using a panel targeting 54 genes recurrently affected in myeloid disorders (
TruSight Myeloid panel, Illumina
) showed that the gene profiles of samples from 48 PBSC donors remained stable throughout the study period. These data strongly indicate absence of detrimental effects on the genome integrity caused by PBSC donation.</description><subject>14/32</subject><subject>45/23</subject><subject>631/208/212</subject><subject>631/532</subject><subject>Blood</subject><subject>Blood cancer</subject><subject>Bone marrow</subject><subject>Cell Biology</subject><subject>Chromosome aberrations</subject><subject>Chromosomes</subject><subject>Colony-stimulating factor</subject><subject>Fluorescence</subject><subject>Fluorescence in situ hybridization</subject><subject>Genomes</subject><subject>Granulocyte colony-stimulating factor</subject><subject>Hematology</subject><subject>Hematopoietic stem cells</subject><subject>Integrity</subject><subject>Internal Medicine</subject><subject>Leukemia</subject><subject>Leukocytes (granulocytic)</subject><subject>Long-term effects</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Next-generation sequencing</subject><subject>Peripheral blood</subject><subject>Public Health</subject><subject>Stem cell transplantation</subject><subject>Stem Cells</subject><issn>0268-3369</issn><issn>1476-5365</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kV1rFTEQhoMo9rT6A7yRgDferE6-dy9LsbZQ8MLeh-xmcthyNlszu8L592Y5VaGgEEhm8sw7M7yMvRPwSYBqP5MWGroGJDTQadMcX7Cd0M42Rlnzku1A2rZRynZn7JzoAUBoDeY1O1OyPmq0Y-WyJ8wD8jnxGKawH_OeY0o4LLTlaMGJD3g48DjnsIxz5mPmay54CAvGLTsX4oEI64m8P_Lr2-83POTI95iR_wxlDFsDGgpirvJv2KsUDoRvn-4Ldn_95f7qprn79vX26vKuGZSRS9PZqKLulFMopFIutgGTxSRaEbu6toQ0JGcMiAC6d1K41CUtY5TGSterC_bxJPtY5h8r0uKnkbZNQsZ5JS81WAWVbSv64Rn6MK8l1-Eq5QxobTX8nxJ1HiOdrZQ4UUOZiQom_1jGKZSjF-A31_zJNV9d85tr_lhr3j8pr_2E8U_Fb5sqIE8A1a-8x_K39b9VfwGOPKDc</recordid><startdate>20200701</startdate><enddate>20200701</enddate><creator>Nacheva, E.</creator><creator>Ahyee, T.</creator><creator>Addada, J.</creator><creator>Navarette, C.</creator><creator>Pamphilon, D.</creator><creator>Regan, F.</creator><creator>Pawson, R.</creator><creator>Szydlo, R.</creator><creator>Goldman, J.</creator><creator>Mackinnon, S.</creator><creator>Shaw, B. 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E.</au><au>Madrigal, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Absence of damaging effects of stem cell donation in unrelated donors assessed by FISH and gene variance screening</atitle><jtitle>Bone marrow transplantation (Basingstoke)</jtitle><stitle>Bone Marrow Transplant</stitle><addtitle>Bone Marrow Transplant</addtitle><date>2020-07-01</date><risdate>2020</risdate><volume>55</volume><issue>7</issue><spage>1290</spage><epage>1296</epage><pages>1290-1296</pages><issn>0268-3369</issn><eissn>1476-5365</eissn><abstract>Astract
Granulocyte–Colony-Stimulating factor (G-CSF) is currently the standard mobilising agent for peripheral blood stem cell (PBSC) donation. Concerns that it may trigger chromosome aberrations similar to those observed in leukaemia patients were refuted but long-term effects of G-CSF mobilisation on genome integrity remains unclear. In the setting of a multi-centre clinical trial we screened blood samples from 50 PBSC donors at cellular and gene level for aberrations common in haematological malignancies using fluorescence in situ hybridisation (FISH) and next generation sequencing (NGS) assays. Analysis of samples collected before, on the day of donation, 90 and 180 days after G-CSF admission confirmed the absence of short-term effects in PBSC donors on both quiescent and dividing cells. This data did not differ from the results of 50 individuals tested 3–5 years after bone marrow donation and 50 healthy persons. NGS using a panel targeting 54 genes recurrently affected in myeloid disorders (
TruSight Myeloid panel, Illumina
) showed that the gene profiles of samples from 48 PBSC donors remained stable throughout the study period. These data strongly indicate absence of detrimental effects on the genome integrity caused by PBSC donation.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>32440014</pmid><doi>10.1038/s41409-020-0945-y</doi><tpages>7</tpages></addata></record> |
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| ispartof | Bone marrow transplantation (Basingstoke), 2020-07, Vol.55 (7), p.1290-1296 |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SpringerLink Journals - AutoHoldings |
| subjects | 14/32 45/23 631/208/212 631/532 Blood Blood cancer Bone marrow Cell Biology Chromosome aberrations Chromosomes Colony-stimulating factor Fluorescence Fluorescence in situ hybridization Genomes Granulocyte colony-stimulating factor Hematology Hematopoietic stem cells Integrity Internal Medicine Leukemia Leukocytes (granulocytic) Long-term effects Medicine Medicine & Public Health Next-generation sequencing Peripheral blood Public Health Stem cell transplantation Stem Cells |
| title | Absence of damaging effects of stem cell donation in unrelated donors assessed by FISH and gene variance screening |
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