Comparison of serologic status of Toxoplasma gondii infection in pre‐ and post‐heart transplantation in a pediatric population: A preliminary study
Background Toxoplasmosis is an important opportunistic infection in immunocompromised children, especially in heart transplant recipients. This study aimed to investigate pre‐ and post‐transplant serology for toxoplasmosis along with post‐transplant PCR in pediatric heart transplant patients. Method...
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| Veröffentlicht in: | Transplant infectious disease 2020-08, Vol.22 (4), p.e13339-n/a |
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| creator | Orang, Elahe Sayyahfar, Shirin Mahdavi, Mohammad Khanaliha, Khadijeh Amiri, Mehri |
| description | Background
Toxoplasmosis is an important opportunistic infection in immunocompromised children, especially in heart transplant recipients. This study aimed to investigate pre‐ and post‐transplant serology for toxoplasmosis along with post‐transplant PCR in pediatric heart transplant patients.
Methods
This cross‐sectional study was performed on 38 heart transplant recipients aged 1‐17 years, by the end of 2018. Pre‐ and post‐transplant IgM and IgG titrations were measured using ELISA method. Nested PCR of B1 gene was performed to identify Toxoplasma gondii (T gondii) infection after transplant.
Results
Totally, 11.4% of patients had positive IgG and 91.4% had negative IgM for toxoplasmosis before heart transplantation. The mean of pre‐transplant IgG titration for seropositive and seronegative patients was 22.32 ± 15.30 IU/mL and 1.49 ± 1.15 IU/mL, respectively (P 12 months (1.07 ± 1.27 IU/mL; P = .004) time periods. The result of PCR for B1 gene in all cases was negative.
Conclusions
Chemoprophylaxis with TMP/SMX seems to be effective in prevention of T gondii infection or reactivation among pediatric heart transplantation population. Anti‐T. gondii‐IgG level alone may not be sensitive enough for evaluation of the infection at least after 6 months post‐transplantation. |
| doi_str_mv | 10.1111/tid.13339 |
| format | Article |
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Toxoplasmosis is an important opportunistic infection in immunocompromised children, especially in heart transplant recipients. This study aimed to investigate pre‐ and post‐transplant serology for toxoplasmosis along with post‐transplant PCR in pediatric heart transplant patients.
Methods
This cross‐sectional study was performed on 38 heart transplant recipients aged 1‐17 years, by the end of 2018. Pre‐ and post‐transplant IgM and IgG titrations were measured using ELISA method. Nested PCR of B1 gene was performed to identify Toxoplasma gondii (T gondii) infection after transplant.
Results
Totally, 11.4% of patients had positive IgG and 91.4% had negative IgM for toxoplasmosis before heart transplantation. The mean of pre‐transplant IgG titration for seropositive and seronegative patients was 22.32 ± 15.30 IU/mL and 1.49 ± 1.15 IU/mL, respectively (P < .001). All cases were on chemoprophylaxis with trimethoprim‐sulfamethoxazole (TMP/SMX). The mean of post‐transplant IgG titration was 1.62 ± 1.87 IU/mL, which was negative for all cases. Investigating pre‐transplant, IgM titration, 5.7% were positive, 91.4% were negative, and 2.9% were borderline. All cases were post‐transplant IgM negative. The mean of post‐transplant IgG titrations was significantly higher in the first 6 months (3.26 ± 2.68 IU/mL) compared to 6‐12 (1.30 ± 1.34 IU/mL; P = .039) and > 12 months (1.07 ± 1.27 IU/mL; P = .004) time periods. The result of PCR for B1 gene in all cases was negative.
Conclusions
Chemoprophylaxis with TMP/SMX seems to be effective in prevention of T gondii infection or reactivation among pediatric heart transplantation population. Anti‐T. gondii‐IgG level alone may not be sensitive enough for evaluation of the infection at least after 6 months post‐transplantation.</description><identifier>ISSN: 1398-2273</identifier><identifier>EISSN: 1399-3062</identifier><identifier>DOI: 10.1111/tid.13339</identifier><identifier>PMID: 32445414</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Activation ; B1 gene ; Enzyme-linked immunosorbent assay ; heart transplant ; Heart transplantation ; Heart transplants ; Immunoglobulin G ; Immunoglobulin M ; Infections ; Opportunist infection ; Pediatrics ; Population studies ; post‐transplant ; Sensitivity analysis ; Serology ; Sulfamethoxazole ; Titration ; Toxoplasma gondii ; Toxoplasmosis ; Transplantation ; Trimethoprim ; Vitamin E</subject><ispartof>Transplant infectious disease, 2020-08, Vol.22 (4), p.e13339-n/a</ispartof><rights>2020 Wiley Periodicals LLC</rights><rights>This article is protected by copyright. All rights reserved.</rights><rights>2020 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2689-32929176615f3afebe12b9814d627bd75bb940a2ccca1e860f98c5f0279805023</citedby><cites>FETCH-LOGICAL-c2689-32929176615f3afebe12b9814d627bd75bb940a2ccca1e860f98c5f0279805023</cites><orcidid>0000-0002-2883-2736 ; 0000-0003-3264-8496 ; 0000-0001-7087-0288 ; 0000-0002-7772-8353 ; 0000-0001-5437-7661</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Ftid.13339$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Ftid.13339$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32445414$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Orang, Elahe</creatorcontrib><creatorcontrib>Sayyahfar, Shirin</creatorcontrib><creatorcontrib>Mahdavi, Mohammad</creatorcontrib><creatorcontrib>Khanaliha, Khadijeh</creatorcontrib><creatorcontrib>Amiri, Mehri</creatorcontrib><title>Comparison of serologic status of Toxoplasma gondii infection in pre‐ and post‐heart transplantation in a pediatric population: A preliminary study</title><title>Transplant infectious disease</title><addtitle>Transpl Infect Dis</addtitle><description>Background
Toxoplasmosis is an important opportunistic infection in immunocompromised children, especially in heart transplant recipients. This study aimed to investigate pre‐ and post‐transplant serology for toxoplasmosis along with post‐transplant PCR in pediatric heart transplant patients.
Methods
This cross‐sectional study was performed on 38 heart transplant recipients aged 1‐17 years, by the end of 2018. Pre‐ and post‐transplant IgM and IgG titrations were measured using ELISA method. Nested PCR of B1 gene was performed to identify Toxoplasma gondii (T gondii) infection after transplant.
Results
Totally, 11.4% of patients had positive IgG and 91.4% had negative IgM for toxoplasmosis before heart transplantation. The mean of pre‐transplant IgG titration for seropositive and seronegative patients was 22.32 ± 15.30 IU/mL and 1.49 ± 1.15 IU/mL, respectively (P < .001). All cases were on chemoprophylaxis with trimethoprim‐sulfamethoxazole (TMP/SMX). The mean of post‐transplant IgG titration was 1.62 ± 1.87 IU/mL, which was negative for all cases. Investigating pre‐transplant, IgM titration, 5.7% were positive, 91.4% were negative, and 2.9% were borderline. All cases were post‐transplant IgM negative. The mean of post‐transplant IgG titrations was significantly higher in the first 6 months (3.26 ± 2.68 IU/mL) compared to 6‐12 (1.30 ± 1.34 IU/mL; P = .039) and > 12 months (1.07 ± 1.27 IU/mL; P = .004) time periods. The result of PCR for B1 gene in all cases was negative.
Conclusions
Chemoprophylaxis with TMP/SMX seems to be effective in prevention of T gondii infection or reactivation among pediatric heart transplantation population. Anti‐T. gondii‐IgG level alone may not be sensitive enough for evaluation of the infection at least after 6 months post‐transplantation.</description><subject>Activation</subject><subject>B1 gene</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>heart transplant</subject><subject>Heart transplantation</subject><subject>Heart transplants</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin M</subject><subject>Infections</subject><subject>Opportunist infection</subject><subject>Pediatrics</subject><subject>Population studies</subject><subject>post‐transplant</subject><subject>Sensitivity analysis</subject><subject>Serology</subject><subject>Sulfamethoxazole</subject><subject>Titration</subject><subject>Toxoplasma gondii</subject><subject>Toxoplasmosis</subject><subject>Transplantation</subject><subject>Trimethoprim</subject><subject>Vitamin E</subject><issn>1398-2273</issn><issn>1399-3062</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp1kbtOBCEUhonRuOul8AUMiY0Wq1zmht1mvSab2Kz1hGEYZTMDCEx0Ox_BzvfzSWQvWphIA4HvfJyTH4AjjM5xXBdB1eeYUsq2wBBTxkYUZWR7dS5GhOR0APa8nyOEc5awXTCgJEnSBCdD8DkxneVOeaOhaaCXzrTmSQnoAw-9X97NzJuxLfcdh09G10pBpRspgoolSkPr5Nf7B-S6htb4EM_PkrsAg-PaxzodRRuUQytrxYOLfmts365eLuF4KWlVpzR3i_hzXy8OwE7DWy8PN_s-eLy5nk3uRtOH2_vJeDoSJCvioIQRhvMsw2lDeSMriUnFCpzUGcmrOk-riiWIEyEEx7LIUMMKkTaI5KxAKSJ0H5yuvdaZl176UHbKC9nGvqXpfUkSlFGU4myJnvxB56Z3OnYXKYpYihFGkTpbU8IZ751sSutUF-cqMSqXaZUxrXKVVmSPN8a-6mT9S_7EE4GLNfCqWrn431TO7q_Wym8alaI9</recordid><startdate>202008</startdate><enddate>202008</enddate><creator>Orang, Elahe</creator><creator>Sayyahfar, Shirin</creator><creator>Mahdavi, Mohammad</creator><creator>Khanaliha, Khadijeh</creator><creator>Amiri, Mehri</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2883-2736</orcidid><orcidid>https://orcid.org/0000-0003-3264-8496</orcidid><orcidid>https://orcid.org/0000-0001-7087-0288</orcidid><orcidid>https://orcid.org/0000-0002-7772-8353</orcidid><orcidid>https://orcid.org/0000-0001-5437-7661</orcidid></search><sort><creationdate>202008</creationdate><title>Comparison of serologic status of Toxoplasma gondii infection in pre‐ and post‐heart transplantation in a pediatric population: A preliminary study</title><author>Orang, Elahe ; Sayyahfar, Shirin ; Mahdavi, Mohammad ; Khanaliha, Khadijeh ; Amiri, Mehri</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2689-32929176615f3afebe12b9814d627bd75bb940a2ccca1e860f98c5f0279805023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Activation</topic><topic>B1 gene</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>heart transplant</topic><topic>Heart transplantation</topic><topic>Heart transplants</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulin M</topic><topic>Infections</topic><topic>Opportunist infection</topic><topic>Pediatrics</topic><topic>Population studies</topic><topic>post‐transplant</topic><topic>Sensitivity analysis</topic><topic>Serology</topic><topic>Sulfamethoxazole</topic><topic>Titration</topic><topic>Toxoplasma gondii</topic><topic>Toxoplasmosis</topic><topic>Transplantation</topic><topic>Trimethoprim</topic><topic>Vitamin E</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Orang, Elahe</creatorcontrib><creatorcontrib>Sayyahfar, Shirin</creatorcontrib><creatorcontrib>Mahdavi, Mohammad</creatorcontrib><creatorcontrib>Khanaliha, Khadijeh</creatorcontrib><creatorcontrib>Amiri, Mehri</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transplant infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Orang, Elahe</au><au>Sayyahfar, Shirin</au><au>Mahdavi, Mohammad</au><au>Khanaliha, Khadijeh</au><au>Amiri, Mehri</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of serologic status of Toxoplasma gondii infection in pre‐ and post‐heart transplantation in a pediatric population: A preliminary study</atitle><jtitle>Transplant infectious disease</jtitle><addtitle>Transpl Infect Dis</addtitle><date>2020-08</date><risdate>2020</risdate><volume>22</volume><issue>4</issue><spage>e13339</spage><epage>n/a</epage><pages>e13339-n/a</pages><issn>1398-2273</issn><eissn>1399-3062</eissn><abstract>Background
Toxoplasmosis is an important opportunistic infection in immunocompromised children, especially in heart transplant recipients. This study aimed to investigate pre‐ and post‐transplant serology for toxoplasmosis along with post‐transplant PCR in pediatric heart transplant patients.
Methods
This cross‐sectional study was performed on 38 heart transplant recipients aged 1‐17 years, by the end of 2018. Pre‐ and post‐transplant IgM and IgG titrations were measured using ELISA method. Nested PCR of B1 gene was performed to identify Toxoplasma gondii (T gondii) infection after transplant.
Results
Totally, 11.4% of patients had positive IgG and 91.4% had negative IgM for toxoplasmosis before heart transplantation. The mean of pre‐transplant IgG titration for seropositive and seronegative patients was 22.32 ± 15.30 IU/mL and 1.49 ± 1.15 IU/mL, respectively (P < .001). All cases were on chemoprophylaxis with trimethoprim‐sulfamethoxazole (TMP/SMX). The mean of post‐transplant IgG titration was 1.62 ± 1.87 IU/mL, which was negative for all cases. Investigating pre‐transplant, IgM titration, 5.7% were positive, 91.4% were negative, and 2.9% were borderline. All cases were post‐transplant IgM negative. The mean of post‐transplant IgG titrations was significantly higher in the first 6 months (3.26 ± 2.68 IU/mL) compared to 6‐12 (1.30 ± 1.34 IU/mL; P = .039) and > 12 months (1.07 ± 1.27 IU/mL; P = .004) time periods. The result of PCR for B1 gene in all cases was negative.
Conclusions
Chemoprophylaxis with TMP/SMX seems to be effective in prevention of T gondii infection or reactivation among pediatric heart transplantation population. Anti‐T. gondii‐IgG level alone may not be sensitive enough for evaluation of the infection at least after 6 months post‐transplantation.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>32445414</pmid><doi>10.1111/tid.13339</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-2883-2736</orcidid><orcidid>https://orcid.org/0000-0003-3264-8496</orcidid><orcidid>https://orcid.org/0000-0001-7087-0288</orcidid><orcidid>https://orcid.org/0000-0002-7772-8353</orcidid><orcidid>https://orcid.org/0000-0001-5437-7661</orcidid></addata></record> |
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| subjects | Activation B1 gene Enzyme-linked immunosorbent assay heart transplant Heart transplantation Heart transplants Immunoglobulin G Immunoglobulin M Infections Opportunist infection Pediatrics Population studies post‐transplant Sensitivity analysis Serology Sulfamethoxazole Titration Toxoplasma gondii Toxoplasmosis Transplantation Trimethoprim Vitamin E |
| title | Comparison of serologic status of Toxoplasma gondii infection in pre‐ and post‐heart transplantation in a pediatric population: A preliminary study |
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