Simultaneous calcium recordings of hippocampal CA1 and primary motor cortex M1 and their relations to behavioral activities in freely moving epileptic mice
Epilepsy is a common neurological disorder characterized by recurrent epileptic seizures. The cause of most cases of epilepsy is unknown. Although changes of calcium events in a single brain region during seizures have been reported before, there have been few studies on relations between calcium ev...
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| Veröffentlicht in: | Experimental brain research 2020-06, Vol.238 (6), p.1479-1488 |
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| creator | Dong, Xi Zhang, Xin Wang, Feifei Liu, Nannan Liu, Aili Li, Yuanyuan Wei, Liangpeng Chen, Feng Yuan, Shiyang Zhang, Kai Hou, Shaowei Jiao, Qingyan Hu, Qi Guo, Cunle Wu, Tongrui Wei, Sheng Shen, Hui |
| description | Epilepsy is a common neurological disorder characterized by recurrent epileptic seizures. The cause of most cases of epilepsy is unknown. Although changes of calcium events in a single brain region during seizures have been reported before, there have been few studies on relations between calcium events of two different brain regions and epileptic behaviors in freely moving mice. To analyze calcium events simultaneously recorded in hippocampal CA1 (CA1) and primary motor cortex M1 (M1), and to explore their relations to various epileptic behaviors in freely moving epileptic models. Epileptic models were induced by Kainic acid (KA), a direct agonist of glutamatergic receptor, on adult male C57/BL6J mice. Calcium events of neurons and glia in CA1 and M1 labeled by a calcium indicator dye were recorded simultaneously with a multi-channel fiber photometry system. Three typical types of calcium events associated with KA-induced seizures were observed, including calcium baseline-rising, cortical spreading depression (CSD) and calcium flashing with a steady rate. Our results showed that the calcium baseline-rising occurred in CA1 was synchronized with that in M1, but the CSD waves were not. However, synchronization of calcium flashing in the two areas was uncertain, because it was only detected in CA1. We also observed that different calcium events happened with different epileptic behaviors. Baseline-rising events were accompanied by clonus of forelimbs or trembling, CSD waves were closely related to head movements (15 out of 18, 6 mice). Calcium flashing occurred definitely with drastic convulsive motor seizures (CMS, 6 mice). The results prove that the synchronization of calcium event exists in CA1 and M1, and different calcium events are related with different seizure behaviors. Our results suggest that calcium events involve in the synchronization of neural network and behaviors in epilepsy. |
| doi_str_mv | 10.1007/s00221-020-05815-w |
| format | Article |
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The cause of most cases of epilepsy is unknown. Although changes of calcium events in a single brain region during seizures have been reported before, there have been few studies on relations between calcium events of two different brain regions and epileptic behaviors in freely moving mice. To analyze calcium events simultaneously recorded in hippocampal CA1 (CA1) and primary motor cortex M1 (M1), and to explore their relations to various epileptic behaviors in freely moving epileptic models. Epileptic models were induced by Kainic acid (KA), a direct agonist of glutamatergic receptor, on adult male C57/BL6J mice. Calcium events of neurons and glia in CA1 and M1 labeled by a calcium indicator dye were recorded simultaneously with a multi-channel fiber photometry system. Three typical types of calcium events associated with KA-induced seizures were observed, including calcium baseline-rising, cortical spreading depression (CSD) and calcium flashing with a steady rate. Our results showed that the calcium baseline-rising occurred in CA1 was synchronized with that in M1, but the CSD waves were not. However, synchronization of calcium flashing in the two areas was uncertain, because it was only detected in CA1. We also observed that different calcium events happened with different epileptic behaviors. Baseline-rising events were accompanied by clonus of forelimbs or trembling, CSD waves were closely related to head movements (15 out of 18, 6 mice). Calcium flashing occurred definitely with drastic convulsive motor seizures (CMS, 6 mice). The results prove that the synchronization of calcium event exists in CA1 and M1, and different calcium events are related with different seizure behaviors. Our results suggest that calcium events involve in the synchronization of neural network and behaviors in epilepsy.</description><identifier>ISSN: 0014-4819</identifier><identifier>EISSN: 1432-1106</identifier><identifier>DOI: 10.1007/s00221-020-05815-w</identifier><identifier>PMID: 32424694</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Behavior, Animal - physiology ; Biomedical and Life Sciences ; Biomedicine ; CA1 Region, Hippocampal - metabolism ; CA1 Region, Hippocampal - physiopathology ; Calcium - metabolism ; Calcium signalling ; Convulsions & seizures ; Cortex (motor) ; Cortical spreading depression ; Cortical Spreading Depression - physiology ; Disease Models, Animal ; Epilepsy ; Epilepsy - metabolism ; Epilepsy - physiopathology ; Glutamatergic transmission ; Hippocampus ; Kainic acid ; Male ; Mice ; Mice, Inbred C57BL ; Motor Cortex - metabolism ; Motor Cortex - physiopathology ; Nerve Net - metabolism ; Nerve Net - physiopathology ; Nervous system diseases ; Neural networks ; Neurology ; Neuronal-glial interactions ; Neurons ; Neurophysiology ; Neurosciences ; Photometry ; Research Article ; Seizures ; Seizures (Medicine) ; Synchronization</subject><ispartof>Experimental brain research, 2020-06, Vol.238 (6), p.1479-1488</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>COPYRIGHT 2020 Springer</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c507t-402ece78df4ae7251df14b1d89b87cdd96744c0ae765a5ea59062387a357f63</citedby><cites>FETCH-LOGICAL-c507t-402ece78df4ae7251df14b1d89b87cdd96744c0ae765a5ea59062387a357f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00221-020-05815-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00221-020-05815-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32424694$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dong, Xi</creatorcontrib><creatorcontrib>Zhang, Xin</creatorcontrib><creatorcontrib>Wang, Feifei</creatorcontrib><creatorcontrib>Liu, Nannan</creatorcontrib><creatorcontrib>Liu, Aili</creatorcontrib><creatorcontrib>Li, Yuanyuan</creatorcontrib><creatorcontrib>Wei, Liangpeng</creatorcontrib><creatorcontrib>Chen, Feng</creatorcontrib><creatorcontrib>Yuan, Shiyang</creatorcontrib><creatorcontrib>Zhang, Kai</creatorcontrib><creatorcontrib>Hou, Shaowei</creatorcontrib><creatorcontrib>Jiao, Qingyan</creatorcontrib><creatorcontrib>Hu, Qi</creatorcontrib><creatorcontrib>Guo, Cunle</creatorcontrib><creatorcontrib>Wu, Tongrui</creatorcontrib><creatorcontrib>Wei, Sheng</creatorcontrib><creatorcontrib>Shen, Hui</creatorcontrib><title>Simultaneous calcium recordings of hippocampal CA1 and primary motor cortex M1 and their relations to behavioral activities in freely moving epileptic mice</title><title>Experimental brain research</title><addtitle>Exp Brain Res</addtitle><addtitle>Exp Brain Res</addtitle><description>Epilepsy is a common neurological disorder characterized by recurrent epileptic seizures. The cause of most cases of epilepsy is unknown. Although changes of calcium events in a single brain region during seizures have been reported before, there have been few studies on relations between calcium events of two different brain regions and epileptic behaviors in freely moving mice. To analyze calcium events simultaneously recorded in hippocampal CA1 (CA1) and primary motor cortex M1 (M1), and to explore their relations to various epileptic behaviors in freely moving epileptic models. Epileptic models were induced by Kainic acid (KA), a direct agonist of glutamatergic receptor, on adult male C57/BL6J mice. Calcium events of neurons and glia in CA1 and M1 labeled by a calcium indicator dye were recorded simultaneously with a multi-channel fiber photometry system. Three typical types of calcium events associated with KA-induced seizures were observed, including calcium baseline-rising, cortical spreading depression (CSD) and calcium flashing with a steady rate. Our results showed that the calcium baseline-rising occurred in CA1 was synchronized with that in M1, but the CSD waves were not. However, synchronization of calcium flashing in the two areas was uncertain, because it was only detected in CA1. We also observed that different calcium events happened with different epileptic behaviors. Baseline-rising events were accompanied by clonus of forelimbs or trembling, CSD waves were closely related to head movements (15 out of 18, 6 mice). Calcium flashing occurred definitely with drastic convulsive motor seizures (CMS, 6 mice). The results prove that the synchronization of calcium event exists in CA1 and M1, and different calcium events are related with different seizure behaviors. Our results suggest that calcium events involve in the synchronization of neural network and behaviors in epilepsy.</description><subject>Animals</subject><subject>Behavior, Animal - physiology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>CA1 Region, Hippocampal - metabolism</subject><subject>CA1 Region, Hippocampal - physiopathology</subject><subject>Calcium - metabolism</subject><subject>Calcium signalling</subject><subject>Convulsions & seizures</subject><subject>Cortex (motor)</subject><subject>Cortical spreading depression</subject><subject>Cortical Spreading Depression - physiology</subject><subject>Disease Models, Animal</subject><subject>Epilepsy</subject><subject>Epilepsy - metabolism</subject><subject>Epilepsy - physiopathology</subject><subject>Glutamatergic transmission</subject><subject>Hippocampus</subject><subject>Kainic acid</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Motor Cortex - metabolism</subject><subject>Motor Cortex - physiopathology</subject><subject>Nerve Net - metabolism</subject><subject>Nerve Net - physiopathology</subject><subject>Nervous system diseases</subject><subject>Neural networks</subject><subject>Neurology</subject><subject>Neuronal-glial interactions</subject><subject>Neurons</subject><subject>Neurophysiology</subject><subject>Neurosciences</subject><subject>Photometry</subject><subject>Research Article</subject><subject>Seizures</subject><subject>Seizures (Medicine)</subject><subject>Synchronization</subject><issn>0014-4819</issn><issn>1432-1106</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9ks1u1DAUhS1ERYfCC7BAlpAqWKTYjhMny9GIn0qtkBj2lse5mbhy4mA7U3gWXhZPM7QMqpAXlnW_e2yfexB6RckFJUS8D4QwRjPCSEaKihbZ7RO0oDxnGaWkfIoWhFCe8YrWp-h5CDf7Yy7IM3SaM854WfMF-rU2_WSjGsBNAWtltZl67EE735hhG7BrcWfG0WnVj8ri1ZJiNTR49KZX_ifuXXQeJzrCD3w912IHxicNq6JxQ8DR4Q10amecTwpKR7Mz0UDAZsCtB7B7mV26DcNoLIzRaNwbDS_QSatsgJeH_QytP374tvqcXX35dLlaXmW6ICJmnDDQIKqm5QoEK2jTUr6hTVVvKqGbpi4F55qkWlmoAlRRk5LllVB5IdoyP0NvZ9XRu-8ThCh7EzRYO3siGSe85LxiIqFv_kFv3OSH9LZE0WRuXnPyQG2VBWmG1kWv9F5ULktW5pwLViXq4hEqrQbS390AbbLiuOHdUUNikudxq6YQ5OX66zF7_hfbgbKxC85Od_M4BtkMau9C8NDKw2AlJXIfMjmHTKaQybuQydvU9Ppgw7Tpoblv-ZOqBOQzEFJp2IJ_8Ok_sr8Bi6rcJw</recordid><startdate>20200601</startdate><enddate>20200601</enddate><creator>Dong, Xi</creator><creator>Zhang, Xin</creator><creator>Wang, Feifei</creator><creator>Liu, Nannan</creator><creator>Liu, Aili</creator><creator>Li, Yuanyuan</creator><creator>Wei, Liangpeng</creator><creator>Chen, Feng</creator><creator>Yuan, Shiyang</creator><creator>Zhang, Kai</creator><creator>Hou, Shaowei</creator><creator>Jiao, Qingyan</creator><creator>Hu, Qi</creator><creator>Guo, Cunle</creator><creator>Wu, Tongrui</creator><creator>Wei, Sheng</creator><creator>Shen, Hui</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>0-V</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>88J</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ALSLI</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M2R</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20200601</creationdate><title>Simultaneous calcium recordings of hippocampal CA1 and primary motor cortex M1 and their relations to behavioral activities in freely moving epileptic mice</title><author>Dong, Xi ; Zhang, Xin ; Wang, Feifei ; Liu, Nannan ; Liu, Aili ; Li, Yuanyuan ; Wei, Liangpeng ; Chen, Feng ; Yuan, Shiyang ; Zhang, Kai ; Hou, Shaowei ; Jiao, Qingyan ; Hu, Qi ; Guo, Cunle ; Wu, Tongrui ; Wei, Sheng ; Shen, Hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507t-402ece78df4ae7251df14b1d89b87cdd96744c0ae765a5ea59062387a357f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Behavior, Animal - 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Academic</collection><jtitle>Experimental brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dong, Xi</au><au>Zhang, Xin</au><au>Wang, Feifei</au><au>Liu, Nannan</au><au>Liu, Aili</au><au>Li, Yuanyuan</au><au>Wei, Liangpeng</au><au>Chen, Feng</au><au>Yuan, Shiyang</au><au>Zhang, Kai</au><au>Hou, Shaowei</au><au>Jiao, Qingyan</au><au>Hu, Qi</au><au>Guo, Cunle</au><au>Wu, Tongrui</au><au>Wei, Sheng</au><au>Shen, Hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous calcium recordings of hippocampal CA1 and primary motor cortex M1 and their relations to behavioral activities in freely moving epileptic mice</atitle><jtitle>Experimental brain research</jtitle><stitle>Exp Brain Res</stitle><addtitle>Exp Brain Res</addtitle><date>2020-06-01</date><risdate>2020</risdate><volume>238</volume><issue>6</issue><spage>1479</spage><epage>1488</epage><pages>1479-1488</pages><issn>0014-4819</issn><eissn>1432-1106</eissn><abstract>Epilepsy is a common neurological disorder characterized by recurrent epileptic seizures. The cause of most cases of epilepsy is unknown. Although changes of calcium events in a single brain region during seizures have been reported before, there have been few studies on relations between calcium events of two different brain regions and epileptic behaviors in freely moving mice. To analyze calcium events simultaneously recorded in hippocampal CA1 (CA1) and primary motor cortex M1 (M1), and to explore their relations to various epileptic behaviors in freely moving epileptic models. Epileptic models were induced by Kainic acid (KA), a direct agonist of glutamatergic receptor, on adult male C57/BL6J mice. Calcium events of neurons and glia in CA1 and M1 labeled by a calcium indicator dye were recorded simultaneously with a multi-channel fiber photometry system. Three typical types of calcium events associated with KA-induced seizures were observed, including calcium baseline-rising, cortical spreading depression (CSD) and calcium flashing with a steady rate. Our results showed that the calcium baseline-rising occurred in CA1 was synchronized with that in M1, but the CSD waves were not. However, synchronization of calcium flashing in the two areas was uncertain, because it was only detected in CA1. We also observed that different calcium events happened with different epileptic behaviors. Baseline-rising events were accompanied by clonus of forelimbs or trembling, CSD waves were closely related to head movements (15 out of 18, 6 mice). Calcium flashing occurred definitely with drastic convulsive motor seizures (CMS, 6 mice). The results prove that the synchronization of calcium event exists in CA1 and M1, and different calcium events are related with different seizure behaviors. Our results suggest that calcium events involve in the synchronization of neural network and behaviors in epilepsy.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32424694</pmid><doi>10.1007/s00221-020-05815-w</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Behavior, Animal - physiology Biomedical and Life Sciences Biomedicine CA1 Region, Hippocampal - metabolism CA1 Region, Hippocampal - physiopathology Calcium - metabolism Calcium signalling Convulsions & seizures Cortex (motor) Cortical spreading depression Cortical Spreading Depression - physiology Disease Models, Animal Epilepsy Epilepsy - metabolism Epilepsy - physiopathology Glutamatergic transmission Hippocampus Kainic acid Male Mice Mice, Inbred C57BL Motor Cortex - metabolism Motor Cortex - physiopathology Nerve Net - metabolism Nerve Net - physiopathology Nervous system diseases Neural networks Neurology Neuronal-glial interactions Neurons Neurophysiology Neurosciences Photometry Research Article Seizures Seizures (Medicine) Synchronization |
| title | Simultaneous calcium recordings of hippocampal CA1 and primary motor cortex M1 and their relations to behavioral activities in freely moving epileptic mice |
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