Aldolase C is a novel molecular marker for folliculo-stellate cells in rodent pituitary
The anterior pituitary gland is composed of five types of hormone-producing cells and folliculo-stellate cells. Folliculo-stellate cells do not produce anterior pituitary hormones but they are thought to play important roles as stem cells, phagocytes, or supporting cells of hormone-producing cells i...
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| Veröffentlicht in: | Cell and tissue research 2020-08, Vol.381 (2), p.273-284 |
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| creator | Fujiwara, Ken Tsukada, Takehiro Horiguchi, Kotaro Fujiwara, Yoko Takemoto, Konomi Nio-Kobayashi, Junko Ohno, Nobuhiko Inoue, Kinji |
| description | The anterior pituitary gland is composed of five types of hormone-producing cells and folliculo-stellate cells. Folliculo-stellate cells do not produce anterior pituitary hormones but they are thought to play important roles as stem cells, phagocytes, or supporting cells of hormone-producing cells in the anterior pituitary. S100β protein has been used as a folliculo-stellate cell marker in some animals, including rats. However, since no reliable molecular marker for folliculo-stellate cells has been reported in mice, genetic approaches for the investigation of folliculo-stellate cells in mice are not yet available. Aldolase C/Zebrin II is a brain-type isozyme and is a fructose-1,6-bisphosphate aldolase. In the present study, we first used immunohistochemistry to verify that aldolase C was produced in the anterior pituitary of rats. Moreover, using transgenic rats expressing green fluorescent protein under the control of the
S100β
gene promoter, we identified aldolase C-immunoreactive signals in folliculo-stellate cells and marginal cells located in the parenchyma of the anterior pituitary and around Rathke’s cleft, respectively. We also identified aldolase C-expressing cells in the mouse pituitary using immunohistochemistry and in situ hybridization. Aldolase C was not produced in any pituitary hormone-producing cells, while aldolase C-immunopositive signal co-localized with E-cadherin- and SOX2-positive cells. Using post-embedding immunoelectron microscopy, aldolase C-immunoreactive products were observed in the cytoplasm of marginal cells and folliculo-stellate cells of the mouse pituitary. Taken together, aldolase C is a common folliculo-stellate cell marker in the anterior pituitary gland of rodents. |
| doi_str_mv | 10.1007/s00441-020-03200-1 |
| format | Article |
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S100β
gene promoter, we identified aldolase C-immunoreactive signals in folliculo-stellate cells and marginal cells located in the parenchyma of the anterior pituitary and around Rathke’s cleft, respectively. We also identified aldolase C-expressing cells in the mouse pituitary using immunohistochemistry and in situ hybridization. Aldolase C was not produced in any pituitary hormone-producing cells, while aldolase C-immunopositive signal co-localized with E-cadherin- and SOX2-positive cells. Using post-embedding immunoelectron microscopy, aldolase C-immunoreactive products were observed in the cytoplasm of marginal cells and folliculo-stellate cells of the mouse pituitary. Taken together, aldolase C is a common folliculo-stellate cell marker in the anterior pituitary gland of rodents.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/s00441-020-03200-1</identifier><identifier>PMID: 32418131</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Cytoplasm ; Dogs ; E-cadherin ; Embedding ; Fluorescence ; Fructose ; Fructose-1,6-diphosphate ; Genetic engineering ; Green fluorescent protein ; Human Genetics ; Hybridization ; Immunoelectron microscopy ; Immunohistochemistry ; Molecular Medicine ; Parenchyma ; Phagocytes ; Pituitary (anterior) ; Pituitary gland ; Pituitary hormones ; Proteomics ; Regular Article ; Stellate cells ; Stem cell transplantation ; Stem cells ; Zebrin II</subject><ispartof>Cell and tissue research, 2020-08, Vol.381 (2), p.273-284</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>COPYRIGHT 2020 Springer</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-a412b545a6741f2f99f8054e441f37f7bb858b5f844d93c9887c5f66435c51083</citedby><cites>FETCH-LOGICAL-c473t-a412b545a6741f2f99f8054e441f37f7bb858b5f844d93c9887c5f66435c51083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00441-020-03200-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00441-020-03200-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27906,27907,41470,42539,51301</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32418131$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujiwara, Ken</creatorcontrib><creatorcontrib>Tsukada, Takehiro</creatorcontrib><creatorcontrib>Horiguchi, Kotaro</creatorcontrib><creatorcontrib>Fujiwara, Yoko</creatorcontrib><creatorcontrib>Takemoto, Konomi</creatorcontrib><creatorcontrib>Nio-Kobayashi, Junko</creatorcontrib><creatorcontrib>Ohno, Nobuhiko</creatorcontrib><creatorcontrib>Inoue, Kinji</creatorcontrib><title>Aldolase C is a novel molecular marker for folliculo-stellate cells in rodent pituitary</title><title>Cell and tissue research</title><addtitle>Cell Tissue Res</addtitle><addtitle>Cell Tissue Res</addtitle><description>The anterior pituitary gland is composed of five types of hormone-producing cells and folliculo-stellate cells. Folliculo-stellate cells do not produce anterior pituitary hormones but they are thought to play important roles as stem cells, phagocytes, or supporting cells of hormone-producing cells in the anterior pituitary. S100β protein has been used as a folliculo-stellate cell marker in some animals, including rats. However, since no reliable molecular marker for folliculo-stellate cells has been reported in mice, genetic approaches for the investigation of folliculo-stellate cells in mice are not yet available. Aldolase C/Zebrin II is a brain-type isozyme and is a fructose-1,6-bisphosphate aldolase. In the present study, we first used immunohistochemistry to verify that aldolase C was produced in the anterior pituitary of rats. Moreover, using transgenic rats expressing green fluorescent protein under the control of the
S100β
gene promoter, we identified aldolase C-immunoreactive signals in folliculo-stellate cells and marginal cells located in the parenchyma of the anterior pituitary and around Rathke’s cleft, respectively. We also identified aldolase C-expressing cells in the mouse pituitary using immunohistochemistry and in situ hybridization. Aldolase C was not produced in any pituitary hormone-producing cells, while aldolase C-immunopositive signal co-localized with E-cadherin- and SOX2-positive cells. Using post-embedding immunoelectron microscopy, aldolase C-immunoreactive products were observed in the cytoplasm of marginal cells and folliculo-stellate cells of the mouse pituitary. Taken together, aldolase C is a common folliculo-stellate cell marker in the anterior pituitary gland of rodents.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cytoplasm</subject><subject>Dogs</subject><subject>E-cadherin</subject><subject>Embedding</subject><subject>Fluorescence</subject><subject>Fructose</subject><subject>Fructose-1,6-diphosphate</subject><subject>Genetic engineering</subject><subject>Green fluorescent protein</subject><subject>Human Genetics</subject><subject>Hybridization</subject><subject>Immunoelectron microscopy</subject><subject>Immunohistochemistry</subject><subject>Molecular Medicine</subject><subject>Parenchyma</subject><subject>Phagocytes</subject><subject>Pituitary (anterior)</subject><subject>Pituitary gland</subject><subject>Pituitary hormones</subject><subject>Proteomics</subject><subject>Regular Article</subject><subject>Stellate cells</subject><subject>Stem cell 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Medicine</topic><topic>Parenchyma</topic><topic>Phagocytes</topic><topic>Pituitary (anterior)</topic><topic>Pituitary gland</topic><topic>Pituitary hormones</topic><topic>Proteomics</topic><topic>Regular Article</topic><topic>Stellate cells</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Zebrin II</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujiwara, Ken</creatorcontrib><creatorcontrib>Tsukada, Takehiro</creatorcontrib><creatorcontrib>Horiguchi, Kotaro</creatorcontrib><creatorcontrib>Fujiwara, Yoko</creatorcontrib><creatorcontrib>Takemoto, Konomi</creatorcontrib><creatorcontrib>Nio-Kobayashi, Junko</creatorcontrib><creatorcontrib>Ohno, Nobuhiko</creatorcontrib><creatorcontrib>Inoue, Kinji</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception 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Res</addtitle><date>2020-08-01</date><risdate>2020</risdate><volume>381</volume><issue>2</issue><spage>273</spage><epage>284</epage><pages>273-284</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><abstract>The anterior pituitary gland is composed of five types of hormone-producing cells and folliculo-stellate cells. Folliculo-stellate cells do not produce anterior pituitary hormones but they are thought to play important roles as stem cells, phagocytes, or supporting cells of hormone-producing cells in the anterior pituitary. S100β protein has been used as a folliculo-stellate cell marker in some animals, including rats. However, since no reliable molecular marker for folliculo-stellate cells has been reported in mice, genetic approaches for the investigation of folliculo-stellate cells in mice are not yet available. Aldolase C/Zebrin II is a brain-type isozyme and is a fructose-1,6-bisphosphate aldolase. In the present study, we first used immunohistochemistry to verify that aldolase C was produced in the anterior pituitary of rats. Moreover, using transgenic rats expressing green fluorescent protein under the control of the
S100β
gene promoter, we identified aldolase C-immunoreactive signals in folliculo-stellate cells and marginal cells located in the parenchyma of the anterior pituitary and around Rathke’s cleft, respectively. We also identified aldolase C-expressing cells in the mouse pituitary using immunohistochemistry and in situ hybridization. Aldolase C was not produced in any pituitary hormone-producing cells, while aldolase C-immunopositive signal co-localized with E-cadherin- and SOX2-positive cells. Using post-embedding immunoelectron microscopy, aldolase C-immunoreactive products were observed in the cytoplasm of marginal cells and folliculo-stellate cells of the mouse pituitary. Taken together, aldolase C is a common folliculo-stellate cell marker in the anterior pituitary gland of rodents.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32418131</pmid><doi>10.1007/s00441-020-03200-1</doi><tpages>12</tpages></addata></record> |
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| subjects | Biomedical and Life Sciences Biomedicine Cytoplasm Dogs E-cadherin Embedding Fluorescence Fructose Fructose-1,6-diphosphate Genetic engineering Green fluorescent protein Human Genetics Hybridization Immunoelectron microscopy Immunohistochemistry Molecular Medicine Parenchyma Phagocytes Pituitary (anterior) Pituitary gland Pituitary hormones Proteomics Regular Article Stellate cells Stem cell transplantation Stem cells Zebrin II |
| title | Aldolase C is a novel molecular marker for folliculo-stellate cells in rodent pituitary |
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