Protein-based polyelectrolyte multilayers

The immobilization of proteins to impart specific functions to surfaces is topical for chemical engineering, healthcare and diagnosis. Layer-by-Layer (LbL) self-assembly is one of the most used method to immobilize macromolecules on surfaces. It consists in the alternate adsorption of oppositely charged species, resulting in the formation of a multilayer. This method in principle allows any charged object to be immobilized on any surface, from aqueous solutions. However, when it comes to proteins, the promises of versatility, simplicity and universality that the LbL approach holds are unmet due to the heterogeneity of protein properties. In this review, the literature is analyzed to make a generic approach emerge, with a view to facilitate the LbL assembly of proteins with polyelectrolytes (PEs). In particular, this review aims at guiding the choice of the PE and the building conditions that lead to the successful growth of protein-based multilayered self-assemblies. [Display omitted] •This review formalizes three generic approaches that facilitate the use of the LbL method for protein immobilization.•Find a protein structural analog that was already successfully immobilized in our database.•Predict the conditions that are necessary to grow a film based on the mechanisms of protein-PE LbL assembly.•Use unconventional LbL assemblies